Magnetic Resonance Letters最新文献_第2页

Biophysical and NMR analysis reveals binding affinity between HAX1 and CLPB proteins 生物物理和核磁共振分析显示HAX1和CLPB蛋白之间的结合亲和力

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200141

Huiqin Zhang , Yong Liu , Yunyan Li , Maosen Ruan , Shu Zhou , Junfeng Wang , Jing Yang

{"title":"Biophysical and NMR analysis reveals binding affinity between HAX1 and CLPB proteins","authors":"Huiqin Zhang , Yong Liu , Yunyan Li , Maosen Ruan , Shu Zhou , Junfeng Wang , Jing Yang","doi":"10.1016/j.mrl.2024.200141","DOIUrl":"10.1016/j.mrl.2024.200141","url":null,"abstract":"<div><div>HCLS1-associated protein X-1 (HAX1) is a multifunctional mitochondrial protein involved in the regulation of apoptosis, a crucial process of programmed cell death, and mRNA processing. Despite its significance, limited structural data is available for HAX1, hindering a comprehensive understanding of its biological function. Notably, the caseinolytic mitochondrial matrix peptidase chaperone subunit B (CLPB) has been identified as an interacting partner of HAX1, yet the biophysical properties and binding affinity governing their interaction remain poorly defined. In this study, we present a thorough biophysical characterization of full-length human HAX1 and CLPB, accomplished through recombinant expression and purification. By employing size exclusion chromatography, dynamic light scattering, and circular dichroism spectroscopy, we successfully established their biophysical properties, revealing contrasting structural features, with CLPB displaying α-helical content and HAX1 exhibiting a disordered nature. Moreover, we employed solution-state nuclear magnetic resonance (NMR) spectroscopy to probe their binding affinity. Our findings demonstrate the formation of stable multimeric complexes between HAX1 and CLPB, and we quantified a dissociation constant in the low range of micro-molar for their high affinity interaction. These results lay the foundation for further in-depth investigations into the dynamics and energetics governing the HAX1-CLPB interaction, ultimately contributing to a comprehensive understanding of their functional mechanisms.</div></div>","PeriodicalId":93594,"journal":{"name":"Magnetic Resonance Letters","volume":"5 1","pages":"Article 200141"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloud-magnetic resonance imaging system: In the era of 6G and artificial intelligence 云磁共振成像系统：在6G和人工智能时代

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200138

Yirong Zhou , Yanhuang Wu , Yuhan Su , Jing Li , Jianyu Cai , Yongfu You , Jianjun Zhou , Di Guo , Xiaobo Qu

{"title":"Cloud-magnetic resonance imaging system: In the era of 6G and artificial intelligence","authors":"Yirong Zhou , Yanhuang Wu , Yuhan Su , Jing Li , Jianyu Cai , Yongfu You , Jianjun Zhou , Di Guo , Xiaobo Qu","doi":"10.1016/j.mrl.2024.200138","DOIUrl":"10.1016/j.mrl.2024.200138","url":null,"abstract":"<div><div>Magnetic resonance imaging (MRI) plays an important role in medical diagnosis, generating petabytes of image data annually in large hospitals. This voluminous data stream requires a significant amount of network bandwidth and extensive storage infrastructure. Additionally, local data processing demands substantial manpower and hardware investments. Data isolation across different healthcare institutions hinders cross-institutional collaboration in clinics and research. In this work, we anticipate an innovative MRI system and its four generations that integrate emerging distributed cloud computing, 6G bandwidth, edge computing, federated learning, and blockchain technology. This system is called Cloud-MRI, aiming at solving the problems of MRI data storage security, transmission speed, artificial intelligence (AI) algorithm maintenance, hardware upgrading, and collaborative work. The workflow commences with the transformation of <em>k</em>-space raw data into the standardized Imaging Society for Magnetic Resonance in Medicine Raw Data (ISMRMRD) format. Then, the data are uploaded to the cloud or edge nodes for fast image reconstruction, neural network training, and automatic analysis. Then, the outcomes are seamlessly transmitted to clinics or research institutes for diagnosis and other services. The Cloud-MRI system will save the raw imaging data, reduce the risk of data loss, facilitate inter-institutional medical collaboration, and finally improve diagnostic accuracy and work efficiency.</div></div>","PeriodicalId":93594,"journal":{"name":"Magnetic Resonance Letters","volume":"5 1","pages":"Article 200138"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid imaging of pulmonary ventilation function evaluation with 129Xe MRI 129Xe MRI快速成像评价肺通气功能

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200169

Li Fan, Shiyuan Liu

引用次数: 0

Numerical design of transverse gradient coil with transformed magnetic gradient field over an effective imaging area 有效成像区域上梯度磁场变换的横向梯度线圈的数值设计

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200139

Chaoqun Niu , Hongyi Qu

{"title":"Numerical design of transverse gradient coil with transformed magnetic gradient field over an effective imaging area","authors":"Chaoqun Niu , Hongyi Qu","doi":"10.1016/j.mrl.2024.200139","DOIUrl":"10.1016/j.mrl.2024.200139","url":null,"abstract":"<div><div>Gradient coil is an essential component of a magnetic resonance imaging (MRI) scanner. To achieve high spatial resolution and imaging speed, a high-efficiency gradient coil with high slew rate is required. In consideration of the safety and comfort of the patient, the mechanical stability, acoustic noise and peripheral nerve stimulation (PNS) are also need to be concerned for practical use. In our previous work, a high-efficiency whole-body gradient coil set with a hybrid cylindrical-planar structure has been presented, which offers significantly improved coil performances. In this work, we propose to design this transverse gradient coil system with transformed magnetic gradient fields. By shifting up the zero point of gradient fields, the designed new Y-gradient coil could provide enhanced electromagnetic performances. With more uniform coil winding arrangement, the net torque of the new coil is significantly reduced and the generated sound pressure level (SPL) is lower at most tested frequency bands. On the other hand, the new transverse gradient coil designed with rotated magnetic gradient fields produces considerably reduced electric field in the human body, which is important for the use of rapid MR sequences. It's demonstrated that a safer and patient-friendly design could be obtained by using transformed magnetic gradient fields, which is critical for practical use.</div></div>","PeriodicalId":93594,"journal":{"name":"Magnetic Resonance Letters","volume":"5 1","pages":"Article 200139"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OFC: Outside Front Cover OFC：外封面

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/S2772-5162(25)00006-3

引用次数: 0

In-cell dehydration of sodium manganese hexacyanoferrate cathode revealed by solid-state NMR 固态核磁共振揭示六氰合铁酸钠阴极的电池内脱水现象

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200135

Zonglin Li , Xiaobing Lou , Shinuo Kang , Dingming Liu , Fushan Geng , Ming Shen , Bingwen Hu

引用次数: 0

Light-induced nuclear spin hyperpolarization at high magnetic fields 强磁场下光诱导核自旋超极化

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200158

Yi Ji, Guangjin Hou

引用次数: 0

Distinct association of HRAS and KRAS with Mn2+ ion illustrated by paramagnetic NMR 顺磁核磁共振表明，HRAS和KRAS与Mn2+离子有明显的关联

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200168

Jia-Liang Chen , Xun-Cheng Su

{"title":"Distinct association of HRAS and KRAS with Mn2+ ion illustrated by paramagnetic NMR","authors":"Jia-Liang Chen , Xun-Cheng Su","doi":"10.1016/j.mrl.2024.200168","DOIUrl":"10.1016/j.mrl.2024.200168","url":null,"abstract":"<div><div>Rat sarcoma virus oncogene (RAS) proteins are of crucial oncogenic proteins and are involved in several essential intracellular processes. The RAS protein has an intrinsic metal binding site for Mg<sup>2+</sup>, which is important for the conformational stability of the active site. Recently, it was reported that a second metal ion binding site, located further from the active site in HRAS (Harvey RAS homolog), binds Ca<sup>2+</sup> with millimolar affinity. As one of the most abundant metal ions in cells, Mn<sup>2+</sup> is a potential candidate for the second metal ion binding site in RAS proteins. Here, we examined the interaction of Mn<sup>2+</sup> with HRAS and KRAS (Kirsten RAS homolog) using high resolution NMR spectroscopy. The NMR data showed that both the second metal ion binding site and the switch I and II regions bind Mn<sup>2+</sup> in the RAS proteins. Furthermore, our paramagnetic NMR results disclosed the conformational differences in helix α3 and the following loop between HRAS and KRAS, accompanied by the association with metal ion binding. These results provide new insights into the interaction of RAS proteins and Mn<sup>2+</sup> in the respective biological processes in cells.</div></div>","PeriodicalId":93594,"journal":{"name":"Magnetic Resonance Letters","volume":"5 1","pages":"Article 200168"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in solid-state NMR methods for studying RNA structures and dynamics 固体核磁共振方法研究RNA结构和动力学的进展

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2024.200133

Jinhan He, Xiaole Liu, Shenlin Wang

{"title":"Advances in solid-state NMR methods for studying RNA structures and dynamics","authors":"Jinhan He, Xiaole Liu, Shenlin Wang","doi":"10.1016/j.mrl.2024.200133","DOIUrl":"10.1016/j.mrl.2024.200133","url":null,"abstract":"<div><div>Ribonucleic acid (RNA) structures and dynamics play a crucial role in elucidating RNA functions and facilitating the design of drugs targeting RNA and RNA-protein complexes. However, obtaining RNA structures using conventional biophysical techniques, such as X-ray crystallography and solution nuclear magnetic resonance (NMR), presents challenges due to the inherent flexibility and susceptibility to degradation of RNA. In recent years, solid-state NMR (SSNMR) has rapidly emerged as a promising alternative technique for characterizing RNA structure and dynamics. SSNMR has several distinct advantages, including flexibility in sample states, the ability to capture dynamic features of RNA in solid form, and suitability to character RNAs in various sizes. Recent decade witnessed the growth of <sup>1</sup>H-detected SSNMR methods on RNA, which targeted elucidating RNA topology and base pair dynamics in solid state. They have been applied to determine the topology of RNA segment in human immunodeficiency virus (HIV) genome and the base pair dynamics of riboswitch RNA. These advancements have expanded the utility of SSNMR techniques within the RNA research field. This review provides a comprehensive discussion of recent progress in <sup>1</sup>H-detected SSNMR investigations into RNA structure and dynamics. We focus on the established <sup>1</sup>H-detected SSNMR methods, sample preparation protocols, and the implementation of rapid data acquisition approaches.</div></div>","PeriodicalId":93594,"journal":{"name":"Magnetic Resonance Letters","volume":"5 1","pages":"Article 200133"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Operando EPR imaging of lithium dendrites on the graphite in extreme-fast-charging full cells 极快充电电池中石墨上锂枝晶的操作EPR成像

Magnetic Resonance Letters Pub Date : 2025-02-01 DOI: 10.1016/j.mrl.2025.200177

Jianfeng Li

引用次数: 0