Forensic science international. Animals and environments最新文献

{"title":"Range-wide assessment of a SNP panel for individualization and geolocalization of bigleaf maple (Acer macrophyllum Pursh)","authors":"Richard C. Cronn ,&nbsp;Kristen N. Finch ,&nbsp;Laura L. Hauck ,&nbsp;Meaghan Parker-Forney ,&nbsp;Brook G. Milligan ,&nbsp;Jenélle Dowling ,&nbsp;Adventure Scientists","doi":"10.1016/j.fsiae.2021.100033","DOIUrl":"10.1016/j.fsiae.2021.100033","url":null,"abstract":"<div><p>Illegal logging is a worldwide problem that degrades ecosystems, and even low-risk markets like the United States report ~1000 significant hardwood timber theft cases per year. Due to its high value, bigleaf maple (<em>Acer macrophyllum</em> Pursh) is a common target of timber theft in western North America. Using samples from Washington, USA, a single nucleotide polymorphism [SNP] assay was recently developed to facilitate timber poaching investigations in this species based on the single base extension Agena MassARRAY® genotyping system and 133 loci. Our study expands earlier evaluations to include the full 2000 km latitudinal range of bigleaf maple, using 1142 samples collected from California, USA to British Columbia, CAN. Populations in this study are equivalent to level 3 ecoregions, with sample sizes ranging from 28 (California Coast) to 191 (Washington Coast). Wood-derived DNA concentrations between 0.24 and 6 ng/μL yielded SNP call rates &gt;92%, and DNA concentrations within this range did not influence genotyping call rates. DNA from different tissues showed a weak but significant difference in call rate, with DNA from wood showing a slightly higher call rate than leaves (99.4% vs 97.9%, respectively). Analysis of 108 sample replicates showed that missing genotypes occurred at 0.165% of all loci, and that allelic drop-outs and drop-ins each occurred at frequencies of 0.022% and 0.032%, respectively. Population-specific genotype profile probabilities for Pacific Northwest ecoregions were lower than 1.9×10⁻³³; profile probabilities are higher (less powerful) in Californian trees due to lower variability across these SNP loci. Conversely, the geographic origin of trees from Southern Californian ecoregions could be predicted with lower error (8.5–11.7%) than trees from the Pacific Northwest (36–80%), based on random forest classification. This study provides a rich database for forensic individualization of bigleaf maple across its complete range in western North America.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100033"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937421000329/pdfft?md5=bd9cef07688e7438ae1bd9a2301f72e1&pid=1-s2.0-S2666937421000329-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44624761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first case of forensic entomology applied to a roadkill dog carcass in the Colombian Andes","authors":"Eduardo Amat ,&nbsp;Andrea Bustca ,&nbsp;Juan Carlos Jaramillo-Fayad","doi":"10.1016/j.fsiae.2021.100013","DOIUrl":"https://doi.org/10.1016/j.fsiae.2021.100013","url":null,"abstract":"<div><p>An uncommon case of forensic entomology applied to a roadkill animal is detailed; the minimum period of insect activity (PIA<em>min</em>: 72 h) and the postmortem interval (PMI: ≤48 h) were calculated. Inferences and possible events pre- and postmortem are related and proposed, including the possible explanation of the occurrence of colonizer carrion flies on dry remains. The accelerating effect of vertebrate scavengers on the decomposition stages of the carcass was measured (7.4 time faster). This study is the first forensic entomology case applied to a roadkill animal in the neotropics.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100013"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72258415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporating animal forensics in routine meat inspection in the Philippines","authors":"Enrykie B. Fortajada ,&nbsp;Ian Kendrich C. Fontanilla ,&nbsp;Maria Corazon A. De Ungria","doi":"10.1016/j.fsiae.2021.100020","DOIUrl":"https://doi.org/10.1016/j.fsiae.2021.100020","url":null,"abstract":"<div><p>Robust species identification of unprocessed and processed meat is essential to ensure the safety and quality of food products. Meat adulteration results from the wrong identification of animal sources, contamination of different meats during processing, or intentional meat substitution using those from other species and non-meat products of lower economic value. This review discusses the potential applications of DNA barcoding in routine meat inspections in the Philippines. Developing mini-barcode primer sets to enhance the utility of conventional techniques is critical in adopting DNA barcoding technology as a robust tool for routine inspections of meat sold commercially, including those intended for the halal meat industry. Increasing the ability of the Philippine National Meat Inspection Service to document the number, types, and scope of meat fraud is a step forward in finally using animal forensic science as a valuable component of its regulatory functions for the protection of the meat-consuming public.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100020"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72258416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinguishing gamebird and waterfowl feathers from raptor feathers","authors":"Pepper W. Trail,&nbsp;Ariel M. Gaffney","doi":"10.1016/j.fsiae.2021.100009","DOIUrl":"10.1016/j.fsiae.2021.100009","url":null,"abstract":"","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100009"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"102472645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forensic investigations in a case of aggressive behavior of three dogs: Identification of dietary supplements contamination by metandienone and confirmation by hair tests","authors":"Laurie Gheddar ,&nbsp;Jean-Sébastien Raul ,&nbsp;Pascal Kintz","doi":"10.1016/j.fsiae.2021.100022","DOIUrl":"10.1016/j.fsiae.2021.100022","url":null,"abstract":"<div><p>After observing a modification in behavior of his three dogs, an owner consulted a laboratory to determine if a drug could be involved. Hair strands of each animal were collected from the neck area and sent to the laboratory for anabolic steroid screening. Initial screening by liquid chromatography coupled to high-resolution mass spectrometry identified the presence of metandienone, an anabolic androgenic steroid, in all three samples. In order to quantify metandienone in hair, a specific method was developed using a liquid chromatography to tandem mass spectrometry system. In addition, sixteen animal pharmaceutical products and food supplements that the dogs had received were tested for a possible source of contamination.</p><p>The hair specimens of the dogs tested positive for metandienone at 42, 13 and 32 pg/mg. Final interpretation of these concentrations was difficult as there is no data about metandienone detection in hair collected from dogs. Analysis of the animal pharmaceutical and food supplements demonstrated the presence of metandienone in two supplements, with amounts at 25 and 452 ng/g.</p><p>Simultaneous analyses of the hair of the dogs and their supplements enabled to make an association with the observed aggressive behavior in the dogs. Testing for anabolic steroids in the hair of the dog in case of aggressiveness can be useful to help to understand an unexpected behavior in dogs.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100022"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"96823669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reptile volatilome profiling optimisation: A pathway towards forensic applications","authors":"Amber O. Brown ,&nbsp;Greta J. Frankham ,&nbsp;Barbara H. Stuart ,&nbsp;Maiken Ueland","doi":"10.1016/j.fsiae.2021.100024","DOIUrl":"10.1016/j.fsiae.2021.100024","url":null,"abstract":"<div><p>Reptiles are the most trafficked live taxa in the illegal wildlife trade, in part due to their popularity as an exotic pet. Current methods used to detect these illegally trafficked animals are limited. This study optimised the collection and analysis parameters associated with volatilome collection that will set the foundation for targeted odourant detection methods. This study determined that the dual sorbent type (Tenax® TA and Carbograph 5DT) in combination with 20-min sampling times and 15-min sampling intervals collected the most reproducible reptile volatilome profiles. It was also determined that desorption methods with mid-range desorption flows (20 ml/min), trap temperatures (-15 °C), and mid-range trap desorption (25 ml/min) were most effective in retrieving collected reptile volatilomes. Two-dimensional gas chromatography coupled with time-of-flight mass spectrometry was used for analysis, where combined Rxi-624 Sil MS (mid-polar) first dimension and Stabilwax® (polar) second dimension column sets were selected as the most effective columns for analysing reptile volatilomes. The resultant data collected and analysed using these parameters demonstrated that individual volatilomes from three reptile species were distinct using principal component analysis. In addition, this work highlighted the need for more rigorous statistical methods to determine reptile biomarkers and which compounds most significantly influence volatilome profiles between species.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100024"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"93058836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Barcode as an effective tool in the identification of billfishes (Scombroidei, Teleostei) from exported specimens","authors":"Carlos E. Rodrigues Jr. ,&nbsp;Daniel Eduardo Visciano de Carvalho ,&nbsp;Yasmin Garcia ,&nbsp;Diogo T. Hashimoto ,&nbsp;Fausto Foresti ,&nbsp;Fábio Porto-Foresti","doi":"10.1016/j.fsiae.2021.100028","DOIUrl":"https://doi.org/10.1016/j.fsiae.2021.100028","url":null,"abstract":"<div><p>DNA Barcode were used to identify species Billfishes and swordfish <em>Xiphias gladius</em> appeared as the only billfish species exported in Brazil. This system has been used successfully in the identification of species and samples not characterized, or illegally traded, as well as products from threatened species. The DNA barcode technique was used in the identification of billfish products exported through the terminal of the International Airport of São Paulo. Samples of billfish tissues were collected during the procedures to export the products to the United States and England. DNA sequencing permitted to certify that 100 % of the sampled products inspected were correctly identified as <em>Xiphias gladius</em>, thus confirming the NCM information about the species declared by the exporter. The high index of similarity of the sequences obtained in the samples with the sequences deposited in the Bold Systems and GenBank which is an NCBI platform (National Center for Biotechnology Information) platform for this species allow the validation and adequacy of the technique used, with direct application mainly in the process of inspection of the commercialization and exportation of fishery products.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72257882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mission of Forensic Science International: Animals and Environments","authors":"Rob White","doi":"10.1016/j.fsiae.2020.100001","DOIUrl":"10.1016/j.fsiae.2020.100001","url":null,"abstract":"","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100001"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2020.100001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"111278370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological analysis: A powerful tool in wildlife forensic biology","authors":"Pepper W. Trail","doi":"10.1016/j.fsiae.2021.100025","DOIUrl":"10.1016/j.fsiae.2021.100025","url":null,"abstract":"<div><p>It is a truism that “structure is the first thing that we notice whenever looking at organisms” (Gans 1985), and the description and analysis of structure is the province of the science of morphology. Morphological analysis is a well-established and cost-effective technique for the taxonomic identification of wildlife remains. Despite this, it is under-utilized in wildlife crime investigations for reasons including a shortage of trained specialists, the challenge of accessing reference specimens, and the perceived greater rigor of DNA analysis. This paper reviews the methodology of morphological analysis, addresses perceived challenges, and demonstrates the effectiveness of the technique in wildlife forensic biology casework at the National Fish and Wildlife Forensic Laboratory of the U.S. Fish and Wildlife Service.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"102767185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Society for Wildlife Forensic Science standards and guidelines","authors":"M. Katherine Moore ,&nbsp;Barry W. Baker ,&nbsp;Tasha L. Bauman ,&nbsp;Mary K. Burnham-Curtis ,&nbsp;Edgard O. Espinoza ,&nbsp;Carolyn S. Ferrell ,&nbsp;Greta J. Frankham ,&nbsp;Kim Frazier ,&nbsp;Jenny L. Giles ,&nbsp;Deedra Hawk ,&nbsp;Jeffrine J. Rovie-Ryan ,&nbsp;Rebecca N. Johnson ,&nbsp;Trey Knott ,&nbsp;Irving L. Kornfield ,&nbsp;Christina Lindquist ,&nbsp;Wayne D. Lord ,&nbsp;Kelly L. Morgan ,&nbsp;R. Christopher O’Brien ,&nbsp;Rob Ogden ,&nbsp;Tracey-Leigh Prigge ,&nbsp;Lucy M.I. Webster","doi":"10.1016/j.fsiae.2021.100015","DOIUrl":"10.1016/j.fsiae.2021.100015","url":null,"abstract":"","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100015"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"98477125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}