International journal of biochemistry and biophysics最新文献_第2页

Comparison of Calculated LDL-Cholesterol Using Various Formulae with Directly Measured LDL-Cholesterol: A Retrospective Study 不同公式计算ldl -胆固醇与直接测量ldl -胆固醇的比较:一项回顾性研究

International journal of biochemistry and biophysics Pub Date : 2020-12-01 DOI: 10.13189/IJBB.2020.080301

Kruthi B N, A. Nusrath

{"title":"Comparison of Calculated LDL-Cholesterol Using Various Formulae with Directly Measured LDL-Cholesterol: A Retrospective Study","authors":"Kruthi B N, A. Nusrath","doi":"10.13189/IJBB.2020.080301","DOIUrl":"https://doi.org/10.13189/IJBB.2020.080301","url":null,"abstract":"Background: Among the various risk factors for developing dyslipidemia and Cardiovascular Disease (CVD), elevated Low-Density Lipoprotein Cholesterol (LDL-C) has been recognized as an independent and widely accepted risk factor for CVD. Therefore the objective of the study was to calculate LDL-C using various formulae and to compare the various formulae to calculate LDL-C with direct LDL-C measurement. Materials and methods: It was a retrospective observational study and study was conducted for a period of 3 Months. During this period, 400 consecutive serum samples were included, out of which 37 had TG value more than 400 mg/dl and were excluded from the study. Finally 363 samples data were included for analysis and data were obtained from the investigation ledger of clinical Biochemistry section and same sample Triglyceride, Total Cholesterol and High Density Lipoprotein Cholesterol was used for calculating LDL-C using various formulae. Results: A total of 400 lipid profile reports were collected, of these, 37 reports had triglyceride level ≥ 400 mg/dl and hence 363 samples were included. There is no statistical difference between the direct LDL-C (131.64± 29.34mg/dl) and LDL-C level calculated by Friedewald formula (133.85±35.97mg/dl, p>0.05). There is a highly significant statistical difference between the direct LDL-C and the LDL-C level calculated using all other formulae (p=0.0001) Cordova and Cordova formula (88.06 ± 28.40mg/dl), Vujovic formula (80.44 ± 15.52mg/dl), Ahmadi formula (186.34 ± 63.26mg/dl), Anandaraja formula (92.46 ± 32.37mg/dl), Puavillai formula (88.54 ± 32.78mg/dl) and Hattori formula (77.45 ± 30.31mg/dl). Conclusion: The study established Friedewald formula as the most suitable method for calculating LDL-C in the absence of direct LDL-C measurement facilities. A large sample size and multicentric study is warranted to confirm which formula is most suitable for measuring the LDL-C in the absence of direct LDL-C measurement.","PeriodicalId":93301,"journal":{"name":"International journal of biochemistry and biophysics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47285778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Anti-inflammatory Activity of Crinum scillifolium Extracts in Wistar Rats 红花提取物对Wistar大鼠抗炎作用的评价

International journal of biochemistry and biophysics Pub Date : 2018-12-01 DOI: 10.13189/ijbb.2018.060401

Koua Kadio Brou Donald, I. Geneviève, Effo Kouakou Etienne, K. S. Landry, A. Djadji, Tetchi Achille Fabrice, Y. Félix

{"title":"Evaluation of Anti-inflammatory Activity of Crinum scillifolium Extracts in Wistar Rats","authors":"Koua Kadio Brou Donald, I. Geneviève, Effo Kouakou Etienne, K. S. Landry, A. Djadji, Tetchi Achille Fabrice, Y. Félix","doi":"10.13189/ijbb.2018.060401","DOIUrl":"https://doi.org/10.13189/ijbb.2018.060401","url":null,"abstract":"Aim of study: Inflammation was associated with many diseases in humans. Crinum species have a considerable medicinal reputation as potent folkloric remedies. The main objective of the study was to evaluate the anti-inflammatory activity of aqueous and hydroethanolic extracts of Crinum scillifolium bulbs in in vivo models. Materials and methods: The anti-inflammatory effect of Crinum scillifolium extracts was also evaluated in carrageenan-induced paw edema models and C-reactive protein (CRP) levels was measured. Two doses 100 and 200 mg/kg body weight for each extract, were tested. The results obtained were compared with those of the standard drug (Diclofenac at 25 mg/kg body weight) and those of the control (normal saline). Results: The results showed a highly significant decrease in the edema size (p < 0.01) and significant decrease in CRP values (p < 0.01) compared to control group when the animals were treated with diclofenac at 25 mg/kg, and 200 mg/kg of aqueous and hydroethanolic extracts. Conclusion: The study suggests that the extracts possess enough potential to reduce inflammation on rat model and directs the importance of further research and development of novel anti-inflammatory agents.","PeriodicalId":93301,"journal":{"name":"International journal of biochemistry and biophysics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48343831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Knowledge of Diabetes among Rural High School Children of Mandya Mandya农村高中儿童糖尿病知识调查

International journal of biochemistry and biophysics Pub Date : 2018-08-01 DOI: 10.13189/IJBB.2018.060301

Shilpashree Yd, A. Nusrath, Namitha Devegowda

{"title":"Knowledge of Diabetes among Rural High School Children of Mandya","authors":"Shilpashree Yd, A. Nusrath, Namitha Devegowda","doi":"10.13189/IJBB.2018.060301","DOIUrl":"https://doi.org/10.13189/IJBB.2018.060301","url":null,"abstract":"Background and objectives: Diabetes mellitus (DM) is one of the most commonly encountered diseases by the healthcare professionals. Knowledge about diabetes can assist in early detection and can reduce the incidence of complication. The present study aimed to evaluate the knowledge of rural school children regarding diabetes mellitus and to identify the areas of gap in the knowledge of diabetes among these children. Materials and Methods: A cross sectional questionnaire based study was conducted. Pretested questionnaire was used to assess the gap in knowledge of diabetes. The questionnaire was administered to all the high school children who consented, from the schools in and around B G Nagara. Data was tabulated and analysed using excel sheet. Results: A total of 302 students participated in the study. In this study, high school children had \"General knowledge about diabetes\" scores of 54.3%, \"Knowledge of life style and non-medical measures\" scores of 50.33%, \"Knowledge of clinical symptoms and complications\" scores of 47.35%, and \"Knowledge of monitoring and treatment of diabetes\" scores of 22.18%. Conclusion: The high school children in this study had fair awareness of diabetes in certain aspects, even though there were many gaps in other areas of knowledge. The awareness of high school children about the disease can be of great help to reduce the risk of developing diabetes and its related complications in future.","PeriodicalId":93301,"journal":{"name":"International journal of biochemistry and biophysics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47063969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Binding Interaction between N,N-Bis (Phosphinomethyl) Amines as a New Class of 1-Aminophosphinic Acids and Bovine Serum Albumin Using Fluorescence Spectroscopy 荧光光谱法分析新型1-氨基膦酸N,N-二(磷甲基)胺与牛血清白蛋白的结合相互作用

International journal of biochemistry and biophysics Pub Date : 2014-08-01 DOI: 10.13189/IJBB.2014.020302

B. Kaboudin, M. R. Faghihi, F. Mohammadi, T. Yokomatsu

{"title":"Analysis of Binding Interaction between N,N-Bis (Phosphinomethyl) Amines as a New Class of 1-Aminophosphinic Acids and Bovine Serum Albumin Using Fluorescence Spectroscopy","authors":"B. Kaboudin, M. R. Faghihi, F. Mohammadi, T. Yokomatsu","doi":"10.13189/IJBB.2014.020302","DOIUrl":"https://doi.org/10.13189/IJBB.2014.020302","url":null,"abstract":"C2-symmetric N,N-bis(phosphinomethyl) amines have been synthesized and their interaction with bovine serum albumin (BSA) was investigated using fluorescence quenching technique. The fluorescence quenching of BSA during its binding to C2-symmetric N,N-bis(phosphinomethyl)amines molecules indicated the occurrence of energy transfer between ligand and protein. The experimental results showed that the formation of aminophosphinic acid-BSA complex and non-radiative energy transferring result in the fluorescence quenching. The binding parameters including binding constant KA and the corresponding thermodynamic parameters were calculated at different temperatures. The thermodynamic investigation showed that the binding process of the C2-symmetric N,N-bis(phosphinomethyl)amines molecules to BSA was a spontaneous molecular interaction procedure in which Gibbs free energy decreased and entropy increased. The hydrophobic interaction force plays a major role in stabilizing of the C2-symmetric N,N-bis(phosphinomethyl)amine-BSA complex. The synchronous fluorescence spectroscopy was used to study the effect of the C2-symmetric N,N-bis(phosphinomethyl)amine on the conformation of BSA. The results obtained from synchronous fluorescence spectra showed that the C2-symmetric N,N-bis(phosphinomethyl)amines did not cause considerable conformational changes in BSA.","PeriodicalId":93301,"journal":{"name":"International journal of biochemistry and biophysics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66235643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Total Bile Acid and Aminotransferases in HIV/AIDS Patients with Coinfection of Hepatitis B and C Viruses 乙肝和丙肝合并感染的HIV/AIDS患者总胆汁酸和转氨酶的测定

International journal of biochemistry and biophysics Pub Date : 2014-05-01 DOI: 10.13189/ijbb.2014.020201

Mathew Folaranmi Olaniyan

引用次数: 1