Vaccination research : open journal最新文献

Intranasal Delivery of Liposomes-Based Lipopeptide Hookworm Vaccine Diminished its Ability to Protect Mice Against Hookworm Challenge 以脂质体为基础的脂肽钩虫疫苗鼻内递送降低了其保护小鼠免受钩虫攻击的能力

Vaccination research : open journal Pub Date : 2022-12-30 DOI: 10.17140/vroj-6-118

Stacey Bartlett, R. Eichenberger, Ahmed O Shalash, A. Loukas, M. Skwarczynski, I. Toth, W. M. Hussein

{"title":"Intranasal Delivery of Liposomes-Based Lipopeptide Hookworm Vaccine Diminished its Ability to Protect Mice Against Hookworm Challenge","authors":"Stacey Bartlett, R. Eichenberger, Ahmed O Shalash, A. Loukas, M. Skwarczynski, I. Toth, W. M. Hussein","doi":"10.17140/vroj-6-118","DOIUrl":"https://doi.org/10.17140/vroj-6-118","url":null,"abstract":"Background Hookworm infection is particularly problematic for middle- to low-income countries. While treatment methods are currently available, vaccination may be the ideal intervention, as it could offer cost-effective long-term protection against infection and reinfection. Methods Previously established lipopeptide-based vaccine formulations, proven to be effective in an oral application, were adapted for an intranasal administration using a predicted B-cell peptide epitope derived from the hookworm Necator americanus aspartic protease-1 (Na-APR-1) protein and a universal T-helper epitope attached to two lipid moieties, Pam2Cys or lipid core peptide (LCP). The lipopeptides were encapsulated into liposomes or self-assembled into nanoparticles. The intranasal vaccine candidates were evaluated in a rodent hookworm challenge model. Results The vaccine candidates were formulated to optimal sizes and charges for uptake by immune cells. However, no significant serum antibody response was elicited, and no protection was demonstrated following hookworm challenge. Conclusion In contrast to the previously reported effective oral immunization, intranasal delivery of lipopeptide-based vaccine failed to trigger significant antibody responses in mice against hookworm and had no effect on parasite numbers following challenge infection.","PeriodicalId":93237,"journal":{"name":"Vaccination research : open journal","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76565750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Future Direction of Cancer Vaccines: An Editorial 癌症疫苗的未来方向:一篇社论

Vaccination research : open journal Pub Date : 2022-12-30 DOI: 10.17140/vroj-6-e007

N. Khansari

引用次数: 0

Model Liposomal Delivery System for Drugs and Vaccines 药物和疫苗的模型脂质体递送系统

Vaccination research : open journal Pub Date : 2020-12-31 DOI: 10.17140/vroj-5-117

F. Firdaus, Zeinab G. Khalil, R. Capon, M. Skwarczynski, I. Toth

引用次数: 1

Recombinant Protein D from Haemophilus influenzae Induces Mouse Bactericidal Antibodies Against Typeable and Non-Typeable Haemophilus influenzae, which Partially Protect Infant Rats Against Serotype b Bacteraemia 流感嗜血杆菌重组蛋白D诱导小鼠抗可分型和不可分型流感嗜血杆菌的杀菌抗体，部分保护幼鼠抗血清型b型菌血症

Vaccination research : open journal Pub Date : 2020-12-31 DOI: 10.17140/vroj-5-116

N. Palmer, Yajun Tan, Manolya Saydam, Arif Felek, Huajie Zhang, Shumin Zhang, Min Fang, J. Wheeler, Qiming Hou, Xiao Ma, Junzhi Wang, F. Mawas

{"title":"Recombinant Protein D from Haemophilus influenzae Induces Mouse Bactericidal Antibodies Against Typeable and Non-Typeable Haemophilus influenzae, which Partially Protect Infant Rats Against Serotype b Bacteraemia","authors":"N. Palmer, Yajun Tan, Manolya Saydam, Arif Felek, Huajie Zhang, Shumin Zhang, Min Fang, J. Wheeler, Qiming Hou, Xiao Ma, Junzhi Wang, F. Mawas","doi":"10.17140/vroj-5-116","DOIUrl":"https://doi.org/10.17140/vroj-5-116","url":null,"abstract":"Aim To evaluate the immunogenicity of a recombinant protein D from Haemophilus Influenzae (Hi) and the functional activities of the induced protein D antibodies in a mouse model. Methods Female Balb/c mice were immunised subcutaneously with recombinant protein D in the presence or absence of adjuvants and the serum immunoglobulin G (IgG) response to protein D was assessed by ELISA. The functional activity of the immune sera was evaluated in vitro using bactericidal assay against typeable Hi serotype b (Hib) and non-typeable Hi (NTHi) clinical isolates and in vivo using an infant rat bacteraemia model and a Hib clinical isolate. Results A dose-dependent IgG response was induced in mice immunised with the recombinant protein D and this response was further increased by the adjuvants used [CPG, AlPO4 and Al(OH)3], with the latter showing the greatest effect on the antibody response. Immune sera were very effective in bactericidal assay against several Hib and NTHi clinical isolates, with a higher serum bactericidal titre against the NTHi than against the Hib isolates. This is possibly due to the lower expression of protein D on the Hib isolates used in our study, compared to the NTHi isolates. In addition, anti-protein D antibodies were partially protective in vivo infant rat bacteraemia model against a challenge with Hib Eagan strain. Conclusion Our results suggest that recombinant protein D is a good vaccine candidate against Hi and should be given in combination with other vaccine candidates to ensure complete protection against Hib and NTHi.","PeriodicalId":93237,"journal":{"name":"Vaccination research : open journal","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88627286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Antibiotic Resistance of the Probiotic Bacteria Found in Commercial Food Products 商品食品中益生菌的抗生素耐药性分析

Vaccination research : open journal Pub Date : 2019-12-31 DOI: 10.17140/vroj-4-115

R. Priyadarshana, Clarencia R. Daniel

{"title":"Analysis of Antibiotic Resistance of the Probiotic Bacteria Found in Commercial Food Products","authors":"R. Priyadarshana, Clarencia R. Daniel","doi":"10.17140/vroj-4-115","DOIUrl":"https://doi.org/10.17140/vroj-4-115","url":null,"abstract":"Aim The Lactobacillus is an industrially-important group of probiotic organisms that plays an important role in human health by inhibiting harmful and pathogenic bacterial growth, boosting immune function, and increasing resistance to infection. The aim of this study was to identify the probiotic bacteria Lactobacillus based on their phenotypic features and genotypic features. This study also shows the importance of probiotic bacterium, and the effects of their antibiotic resistance to human. Method Six different brands were cultured on man, rogosa and sharpe (MRS) agar. The identity of the culture was based on the characteristics of the strains of Lactobacillus spp. which was characterized using their phenotypic features (cell morphology, Gram’s staining tests which are specific for Lactobacillus genus). The bacterial deoxyribonucleic acid (DNA) was extracted by two different methods, boiled cell method and cetyl trimethylammonium bromide (CTAB) method. Furthermore, the extracted DNA yields were compared to determine which gives the best yield. The bacterial genus was detected with using genus specific primers, specific to the Lactobacillus. All the isolates were further subjected to antibiotic resistance test using disc diffusion method against a total of 4 antibiotics (Erythromycin, Tetracycline, Vancomycin and Ampicillin) and the antibiotic resistant genes of tet(M) & erm(B), were analyzed by polymerase chain reaction (PCR). Results Five isolates out of six samples (A to E) were found to exhibit multiple resistance against some of the most commonly used antibiotics. The isolates showed resistance toward tetracycline, erythromycin & vancomycin. Besides that, the isolates displayed a low-level of resistance toward ampicillin. Conclusion This study proves that antibiotic resistance is present in different species of probiotic strains, which may pose a food safety concern.","PeriodicalId":93237,"journal":{"name":"Vaccination research : open journal","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81774894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Review of Vaccine Efficacy Measures 疫苗有效性指标综述

Vaccination research : open journal Pub Date : 2019-12-31 DOI: 10.17140/vroj-4-110

Sayan Dasgupta

引用次数: 4

Neoantigen: A New Hope for Effective Cancer Immunotherapy 新抗原:癌症有效免疫治疗的新希望

Vaccination research : open journal Pub Date : 2019-12-31 DOI: 10.17140/vroj-4-113

N. Khansari

引用次数: 0

Self-Adjuvanting Peptide Vaccines Against Cervical Cancer 预防子宫颈癌的自佐剂肽疫苗

Vaccination research : open journal Pub Date : 2019-12-31 DOI: 10.17140/vroj-4-114

Hannah Hanh-Hong Truong, W. M. Hussein, Tzu-yu Liu, Zhongfan Jia, J. Wells, M. Monteiro, M. Skwarczynski

{"title":"Self-Adjuvanting Peptide Vaccines Against Cervical Cancer","authors":"Hannah Hanh-Hong Truong, W. M. Hussein, Tzu-yu Liu, Zhongfan Jia, J. Wells, M. Monteiro, M. Skwarczynski","doi":"10.17140/vroj-4-114","DOIUrl":"https://doi.org/10.17140/vroj-4-114","url":null,"abstract":"Background Cervical cancer is a common cause of cancer-related deaths in women worldwide, with a fatality rate second only to breast cancer. Human papillomaviruses (HPVs) are the main causative agents of cervical cancer, and are therefore obvious targets for vaccine development. Although two prophylactic HPV vaccines have been commercialized, therapeutic vaccines against HPVs have not been developed yet. Current vaccine technologies emphasize the power of small particles in targeting immune cells, and particles of 20-50 nm have been reported to induce optimal immune responses against a variety of pathogens and cancers. Methods We synthesized new nanoparticle-based vaccines against cervical cancer by using antigenic 8Qmin peptide epitope derived from HPV-16 E7 protein, a hydrophilic poly-(L-glutamic acid) (PGA) linker, and an 8-arm poly (tert-butyl acrylate) dendrimer-based delivery system (D8). Results Four different peptides containing 8Qmin and PGA of different lengths were successfully synthesized with high yield and purity. These were then conjugated to alkyne-functionalized D8 by copper-catalyzed alkyne-azide cycloaddition “click” reaction. The conjugates self-assembled into nanoparticles, with decreased particle size corresponding to a greater number of Glu units. The four vaccine candidates were tested in C57 black 6 (C57BL/6) mice bearing well-established (7-day-old) tumors to examine their therapeutic effects. Conclusion Interestingly, only one conjugate delayed tumor growth, and montanide adjuvanted antigen, used as a positive control, failed to demonstrate any therapeutic effect.","PeriodicalId":93237,"journal":{"name":"Vaccination research : open journal","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85519254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Consensus Meeting on International Standards for Oral Whole Cell Killed Cholera Vaccines, 17-18 May 2018, Seoul, Republic of Korea 口服全细胞灭活霍乱疫苗国际标准共识会议，2018年5月17日至18日，韩国首尔

Vaccination research : open journal Pub Date : 2019-12-31 DOI: 10.17140/vroj-4-112

L. Odevall, SjoerdG. T. Rijpkema, Dean Smith, Tong Wu, F. Qadri, J. Holmgren, J. Lynch, V. Pavliak

{"title":"Consensus Meeting on International Standards for Oral Whole Cell Killed Cholera Vaccines, 17-18 May 2018, Seoul, Republic of Korea","authors":"L. Odevall, SjoerdG. T. Rijpkema, Dean Smith, Tong Wu, F. Qadri, J. Holmgren, J. Lynch, V. Pavliak","doi":"10.17140/vroj-4-112","DOIUrl":"https://doi.org/10.17140/vroj-4-112","url":null,"abstract":"Cholera causes an estimated 3 million cases of watery diarrhea and 100,000 deaths globally per year. Although the long-term solution for cholera control lies in universal access to safe drinking water and adequate sanitation, Oral Cholera Vaccines (OCVs) are the most cost-effective measure to contain and prevent the disease and are recommended by the World Health Organization (WHO) as part of an integrated strategy to control cholera. Currently, three OCVs are WHO prequalified and two of these are part of the cholera vaccine stockpile. Other OCVs have been developed for national use or are under development. Lipopolysaccharide (LPS) Inhibition enzyme-linked immunosorbent assays (ELISAs) are used by both manufacturers of OCVs and national regulatory agencies (NRAs) for: in process analysis, as a potency assay for drug substance and drug product batch release, and as an indicator of stability during the shelf life of OCVs. To ensure quality and consistency of this assay performed by different OCV manufacturers and NRAs, harmonization of assay reagents by the introduction of WHO International Standards (IS) for essential reagents is desirable. In May 2018, the International Vaccine Institute (IVI) with assistance from National Institute of Biological Standards and Control (NIBSC) and financial support from the Bill and Melinda Gates Foundation (BMGF) organized a meeting with representatives from vaccine manufacturers, NRAs, leading research institutions and independent experts to discuss and share experiences on potency assays for batch release and select reagents of the LPS Inhibition ELISA as candidate WHO ISs.","PeriodicalId":93237,"journal":{"name":"Vaccination research : open journal","volume":"9 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87716816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Salmonella-based Anticancer Vaccines and their Efficacy 基于沙门氏菌的抗癌疫苗及其功效

Vaccination research : open journal Pub Date : 2019-12-31 DOI: 10.17140/vroj-4-111

S. Farashi-Bonab, N. Khansari

引用次数: 1