International journal of cancer research & therapy最新文献_第8页

Increased Musashi-2 and Decreased NUMB Protein Levels Observed in Human Colorectal Cancer are reverted to Normal Levels by ATRA-Induced Cell Differentiation. atra诱导的细胞分化可使人类结直肠癌中Musashi-2蛋白升高和NUMB蛋白下降水平恢复到正常水平。

International journal of cancer research & therapy Pub Date : 2018-01-01 Epub Date: 2018-08-15 DOI: 10.33140/ijcrt/03/02/00003

Lynn M Opdenaker, Ryan Kowash, Gabriel Masters, Bruce M Boman, Tao Zhang, Shirin R Modarai

{"title":"Increased Musashi-2 and Decreased NUMB Protein Levels Observed in Human Colorectal Cancer are reverted to Normal Levels by ATRA-Induced Cell Differentiation.","authors":"Lynn M Opdenaker, Ryan Kowash, Gabriel Masters, Bruce M Boman, Tao Zhang, Shirin R Modarai","doi":"10.33140/ijcrt/03/02/00003","DOIUrl":"https://doi.org/10.33140/ijcrt/03/02/00003","url":null,"abstract":"Background: Musashi stem cell (SC) proteins (MSI-1 & MSI-2) are known to become over expressed during colorectal tumorigenesis in humans and mice. MSI-1 overexpression induces tumorigenesis through Notch activation via inactivation of NUMB. Previous studies also show that MSI-2 overexpression in mice induces intestinal tumorigenesis but the mechanism is independent of NUMB. However, whether the MSI-2/NUMB pathway contributes to colorectal cancer (CRC) development in humans is still undetermined.Methods: We evaluated expression of MSI-2 and NUMB proteins in matched normal and CRC patient samples, as well as in human CRC cell lines. We also determined whether induction of cellular differentiation by all-trans retinoic acid (ATRA) influences MSI-2 and NUMB expression.Results: Analysis of matched patient tissue samples and CRC cell lines showed that MSI-2 protein expression is significantly increased and NUMB expression is decreased in CRCs compared to the normal colonic tissue. Immunostaining of normal and adenomatous colonic epithelium revealed that MSI-1+ andMSI-2+ SCs reside in the SC niche and they become overpopulated during colon tumorigenesis. Moreover, promoting cellular differentiation by ATRA reduces MSI-2 protein levels, while increasing NUMB protein levels in human CRC cell lines.Conclusions: MSI-2/NUMB protein expression is altered during colon tumorigenesis, and indicates that MSI-2/NUMB signaling in human colonic stem cells is closely linked to normal colonic epithelial homeostasis.Implications: The ability to normalize MSI-2/NUMB signaling by inducing differentiation of cancer SCs suggests a novel therapeutic approach for CRC treatment.","PeriodicalId":93106,"journal":{"name":"International journal of cancer research & therapy","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7517600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38427713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Introduction of a Portable and Non-Invasive Technology for Hand and Foot Cooling: a Preclinical Feasibility Study 一种便携式无创手足冷却技术的引入:临床前可行性研究

International journal of cancer research & therapy Pub Date : 1900-01-01 DOI: 10.33140/ijcrt.06.02.05

{"title":"Introduction of a Portable and Non-Invasive Technology for Hand and Foot Cooling: a Preclinical Feasibility Study","authors":"","doi":"10.33140/ijcrt.06.02.05","DOIUrl":"https://doi.org/10.33140/ijcrt.06.02.05","url":null,"abstract":"Introduction: Chemotherapy-induced peripheral neuropathy (CIPN) is caused by damage to neural structures in distal limbs. CIPN can lead to reduced dose or cessation of chemotherapy. Cryotherapy has shown to be effective in reducing or preventing CIPN in the hands/feet. However, when using ice bath or ice gloves/socks there is no way to maintain the targeted temperature and prevent ice from melting. Also, patients have difficulty tolerating the freezing temperatures over long periods of chemotherapy. The aim of this study was to test the cooling performance of a recently developed non-invasive system that can ultimately replace current cooling methods. Materials and Methods: COOLPREVENT circulates cold water at tolerable temperatures into malleable gloves/socks that does not require replacing of melted ice. We administered a cooling protocol via COOLPREVENT on three healthy subjects for 60 minutes. Immediately before and after cooling, skin temperature in the hands and feet were measured. Level of discomfort was also recorded during the cooling process. Results: Results showed that COOLPREVENT reduces skin temperature by 14.5±3.8°C and 10.7±1.7°C in the hands and feet, respectively within 60 minutes without significant discomfort. Conclusion: Although our study is limited by the small number of subjects and participation of healthy individuals, but we can conclude that COOLPREVENT can be a safe and appropriate method for hand and foot cooling in a uniform manner. We hope that these preliminary findings can pave the way to designing clinical trials we plan to conduct in the near future.","PeriodicalId":93106,"journal":{"name":"International journal of cancer research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69508317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can we cure our patients in palliative care? 我们能在姑息治疗中治愈病人吗?

International journal of cancer research & therapy Pub Date : 1900-01-01 DOI: 10.33140/ijcrt.06.02.06

引用次数: 0

Consensus guidelines are required for the peri-operative nutritional assessment and management of patients who undergo surgical resection for suspected pancreatic cancer 对于疑似胰腺癌手术切除患者的围手术期营养评估和管理，需要达成共识的指南

International journal of cancer research & therapy Pub Date : 1900-01-01 DOI: 10.33140/ijcrt.06.02.04

引用次数: 0