Bioscientifica proceedings最新文献_第7页

What research is needed to improve commercial pig reproduction 需要进行哪些研究来改善商品猪繁殖

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0038

S. Einarsson

{"title":"What research is needed to improve commercial pig reproduction","authors":"S. Einarsson","doi":"10.1530/biosciprocs.19.0038","DOIUrl":"https://doi.org/10.1530/biosciprocs.19.0038","url":null,"abstract":"Artificial insemination with fresh or stored semen is currently the only sperm technology used at a commercial scale in the pig industry. Attention should therefore be given for further improvement of the functionality and fertilizing ability of cryopreserved semen, as well as for sperm sorting for gender pre-selection. During the last two decades various proteins and polypeptides have been identified in boar seminal plasma, and the relevance of some of them to reproductive technologies has been discussed at this conference. The long- term goal should be to isolate/synthetize those seminal plasma proteins proven important for the spermatozoa, and use them as ingredients in media used for e.g. cryopreservation and sex sorting of semen. A close cooperation between biochemists, molecular biologists and animal scientists is necessary to reach this goal. The brought transcriptional profiling, allowing for the identification of many genes involved e.g. in mammalian gametogenesis, fertilization, and preimplantation embryo development. We do expect further progress within this field of research during coming years. The new information has also capacity to revolutionize the genetic progress within animal breeding. when using single insemination with fresh semen at fixed-time ovulation. deep with lower number, or inseminated","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45793591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Putative role of cocaine- and amphetamine-regulated transcript (CARTPT) in dominant follicle selection in cattle 可卡因和苯丙胺调节转录物（CARPT）在牛优势卵泡选择中的假定作用

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.7.008

George W. Smith, A. Sen, J. Folger, J. Ireland

引用次数: 0

Developmental programming of the ovine placenta 绵羊胎盘发育程序

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.7.004

A. Fowden, J. Ward, F. Wooding, A. Forhead

引用次数: 0

Porcine pluripotent stem cells and their differentiation 猪多能干细胞及其分化

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0028

T. Ezashi, R. M. Roberts

{"title":"Porcine pluripotent stem cells and their differentiation","authors":"T. Ezashi, R. M. Roberts","doi":"10.1530/biosciprocs.19.0028","DOIUrl":"https://doi.org/10.1530/biosciprocs.19.0028","url":null,"abstract":"In some situations, the pig has advantages over the mouse as a model in biomedical research. The availability of pluripotent cell lines is likely to broaden this appeal. Here we review progress in the derivation and characterization of embryonic stem cells (ESC) and induced pluripotent lines (iPSC) from pigs. Until recently, most porcine ESC failed to meet the full criteria for pluripotency, but that may be changing as more becomes known about the culture conditions required to maintain epiblast outgrowths from early porcine conceptuses in an undifferentiated, self-renewing state. In addition, porcine iPSC cells have been generated, some with the features of FGF2-dependent epiblast-type cells, typified by human ESC, and others that require LIF and resemble the “ground state”, na ï ve-type mouse ESC. Despite these successes, incomplete reprogramming and loss of pluripotency when selection conditions are relaxed continue to be problems that must be overcome if the full potential of iPSC is to be realized. The most immediate value of iPSC may relate to their ability to proliferate almost indefinitely in culture, thus enabling more complex genetic manipulations of the genome through growth selection than could be performed in other cell types. The “undifferentiated” state of iPSC may also allow improved cloning efficiency, although this remains to be proved. Finally, the pig will likely prove useful in testing stem cell-based therapies, although only a limited number of experiments demonstrating that the porcine iPSC can be directed to transform into more specialized sub-lineages and then form functional grafts have been performed.","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46729242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The metabolism of the ruminant cumulus-oocyte complex revisited 反刍动物卵丘-卵母细胞复合体的代谢

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.8.022

Jeremy G. Thompson, R. Gilchrist, M. Sutton-Mcdowall

引用次数: 3

Next Generation Sequencing for microRNA profiling in the porcine endometrium 猪子宫内膜microRNA分析的下一代测序

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0019

K. Krawczynski, S. Bauersachs, H. Blum, M. Kaczmarek

引用次数: 0

Judge, jury and executioner: the auto-regulation of luteal function 法官、陪审团和刽子手:黄体功能的自动调节

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.6.012

G. Niswender, T. Davis, Griffith Rj, R. L. Bogan, K. Monser, Rebecca C. Bott, J. Bruemmer, Terry M. Nett

引用次数: 42

Current progress in non-surgical embryo transfer with fresh and vitrified/warmed pig embryos 用新鲜和玻璃化/温热猪胚胎进行非手术胚胎移植的最新进展

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0010

E. Martinez, E. A. Gil, C. Cuello, J. Sanchez-Osorio, J. Gomis, I. Parrilla, M. Angel, H. Rodríguez-Martínez, X. Lucas, J. Vazquez, J. M. Vázquez, J. Roca

{"title":"Current progress in non-surgical embryo transfer with fresh and vitrified/warmed pig embryos","authors":"E. Martinez, E. A. Gil, C. Cuello, J. Sanchez-Osorio, J. Gomis, I. Parrilla, M. Angel, H. Rodríguez-Martínez, X. Lucas, J. Vazquez, J. M. Vázquez, J. Roca","doi":"10.1530/biosciprocs.19.0010","DOIUrl":"https://doi.org/10.1530/biosciprocs.19.0010","url":null,"abstract":"Embryo transfer (ET) should play a critical role in the pig industry because it allows the movement and introduction of new genetic material into a herd with minimal risk of disease transmission and reduced transportation costs. In addition, embryo movement could prevent the potential health and welfare problems associated with transporting live pigs. Although the first successful ET was reported more than 60 years ago, the commercial use of this procedure in pigs is still in its infancy. The surgical requirements for embryo collection and transfer and the difficulties with embryo cryopreservation have prevented its use in pigs, unlike other livestock. However, new methodologies have been developed in the past decade to enable successful non-surgical ET and embryo cryopreservation that could allow the commercial use of ET in the pig industry. This review focuses on the development of these technologies with emphasis on our own findings. Specifically, we discuss the basic aspects of a non-surgical deep-uterine ET procedure and describe several factors that affect its efficacy in the transfer of fresh and short-term cultured embryos. Finally, we conclude with a brief discussion on the use of this procedure with long-term stored embryos.","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48537948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Gene networks in the embryo and endometrium related to embryo survival 胚胎和子宫内膜中与胚胎存活相关的基因网络

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/BIOSCIPROCS.8.006

M. Hoelker, D. Salilew-Wondim, E. Held, D. Tesfaye, K. Schellander

{"title":"Gene networks in the embryo and endometrium related to embryo survival","authors":"M. Hoelker, D. Salilew-Wondim, E. Held, D. Tesfaye, K. Schellander","doi":"10.1530/BIOSCIPROCS.8.006","DOIUrl":"https://doi.org/10.1530/BIOSCIPROCS.8.006","url":null,"abstract":"Low fertility is one of the major problems limiting the efficiency of the dairy industry. Successful pregnancy establishment and development to term depend on the suitability of the endometrial environment and the intrinsic quality of the embryo. Accurate assessment of both, however, is challenging. Therefore, the present survey reviews current knowledge of the molecular networks in the endometrium related to pregnancy establishment and maintenance to term. Accordingly, distinct molecular signatures of the bovine endometrium, elucidated by innovative approaches, could be correlated with pregnancy success. However, not only must the reproductive tract provide a suitable environment but the embryo itself must be competent to express its developmental program. Numerous studies have correlated molecular networks of bovine embryos with their developmental capacity. However, most studies were, by their nature, invasive and the findings were therefore difficult to extrapolate beyond the developmental stages examined. Hence, a second aim is to present new strategies employing embryo biopsies for a direct connection between molecular signatures and embryo developmental capacity. Large scale differences, at the molecular level, were reported for blastomeres whose counterparts developed to the blastocyst stage compared to those with lower developmental competence, thus unraveling distinct molecular fingerprints related to ability to develop to the blastocyst stage. Similarly, distinct molecular signatures at the blastocyst stage were associated with embryo developmental competence to term. In summary, further understanding of molecular signatures related to endometrial receptivity and embryo developmental capacity has been gained from novel innovative strategies including embryo microdissection as well as by examining endometrial samples collected in the cycle preceding the one in which conception occurs.","PeriodicalId":93083,"journal":{"name":"Bioscientifica proceedings","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49029984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining piglet survival 仔猪存活率的测定

Bioscientifica proceedings Pub Date : 2019-04-05 DOI: 10.1530/biosciprocs.19.0014

E. Baxter, S. Edwards

引用次数: 6