Bukovinian Medical Herald最新文献

{"title":"CHARACTERISTICS OF HEART RHYTHM DISORDERS ACCORDING TO THE DATA OF HOLTER ECG MONITORING IN PATIENTS WITH HEART FAILURE AND ATRIAL FIBRILLATION","authors":"N. M. Kulaiets","doi":"10.24061/2413-0737.27.1.105.2023.2","DOIUrl":"https://doi.org/10.24061/2413-0737.27.1.105.2023.2","url":null,"abstract":"Chronic heart failure (CHF) is one of the most significant medical, economic and social problems of the 21st century and remains the main cause of morbidity and mortality worldwide.The purpose. To study the indicators of Holter ECG monitoring, the dynamics of ischemic changes, heart rate, indicators of heart rhythm variability and blood pressure in patients with heart failure and atrial fibrillation.Material and methods. 150 patients aged 45-65 were examined. All patients were divided into groups: Group I – patients with HF with sinus rhythm or atrial fibrillation (AF) (permanent or persistent form), (n=75). Group II – patients without HF with AF, (n=75). Group III – control group: 36 practically healthy people. All patients underwent a clinical examination, ECG, echocardiography (ECHOKG), Holter ECG monitoring (HM ECG) and daily BP monitoring (DMAT).Research results. The analysis of the ECG of the examined patients indicates the presence of heart rhythm disorders, which is an important risk factor and progression of CHF. The results of XM ECG indicate the presence of ventricular arrhythmias of various gradations along with AF. There was a probable difference in the direction of an increase in the number of ventricular extrasystoles (VAS), both single and, most importantly, extrasystoles of high gradations, in patients with CHF and AF compared with healthy subjects. In addition, the number of SHEs in patients with CHF and AF was likely lower than in patients with CHF with sinus rhythm. Indicators of maximum heart rate, ST segment depression and QT interval dispersion in patients with CHF and AF were significantly higher (p<0.05) than similar parameters in patients without CHF with AF.Conclusions: Ventricular heart rhythm disturbances were detected in the examined patients, which is a predictor of an unfavorable prognosis in patients with chronic heart failure. There was a probable difference in the direction of increased levels of maximum heart rate, maximum depression, ST segment elevation, dispersion of the QT interval, QTc, an increase in the total duration of episodes of myocardial ischemia, in patients with chronic heart failure and atrial fibrillation in comparison with healthy subjects.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91349021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EDUCATIONAL AND METHODOLOGICAL FUNDAMENTALS OF TEACHING ISSUES OF GENDER DIFFERENTIATION IN THE DEPARTMENTS OF MORPHOLOGY AT MEDICAL UNIVERSITY","authors":"D. Proniaiev, V. Kryvetskyi, O. Boichuk","doi":"10.24061/2413-0737.27.1.105.2023.14","DOIUrl":"https://doi.org/10.24061/2413-0737.27.1.105.2023.14","url":null,"abstract":"Introduction. The main problem in teaching the processes of gender differentiation during practical classes is that all biochemical and genetically determined processes of gender differentiation are insufficiently covered or detached from the accompanying morphological transformations. Often, educational programs consist only of topics that highlight the morphological processes of the development of a certain sex, avoiding the main questions - the biochemical causes of morphological transformations.The purpose of the research is to find out the current state of studying the problem of teaching the topic \"Gender differentiation\" at the medical and biological faculties of higher educational institutions of Ukraine and highlight our own experience in teaching the topic \"Sex differentiation\" at Bukovinian State Medical University.Material and methods. Educational and methodological practice is based on researched 19 sources of foreign scientific literature from the electronic database \"PubMed\". The methods of comparative content analysis and information-analytical analysis were used.Research results and their discussion. Sex differentiation in humans and other animals is associated with many biochemical processes that occur at the level of genes and proteins. Here are some biochemical aspects of sex differentiation: Hormones are key factors that influence sex differentiation. In boys, the gonads produce testosterone, and in girls, estrogen. These hormones promote the development of various organs and systems, including the reproductive system. Genetic mechanisms: every organism has two sex chromosomes – X and Y. Males have XY, and females have XX. Genes on these chromosomes affect the development of various aspects of sexual differentiation. Proteins produced by genes play an important role in sex differentiation. For example, the SRY protein is responsible for the development of the testicles in boys, while the FOXL2 protein is responsible for the development of the ovaries in girls.Conclusion. The morphological processes that accompany sex differentiation are certainly only a reflection of the complex biochemical transformations that ensure this process. In our opinion, the study of morphological transformations will not have an effect if we consider them without reference to biochemical and genetic processes at the molecular level.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88979681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PECULIARITIES OF ENDOTHELIAL DYSFUNCTION AND CAPILLARY BLOOD FLOW IN PATIENTS WITH COVID-19 CORONAVIRUS DISEASE AND CONCOMITANT TYPE 2 DIABETES MELLITUS","authors":"Z. R. Tylishchak","doi":"10.24061/2413-0737.27.1.105.2023.7","DOIUrl":"https://doi.org/10.24061/2413-0737.27.1.105.2023.7","url":null,"abstract":"The aim of the study was to investigate the indicators of endothelial dysfunction and capillary blood flow in patients with coronavirus disease (COVID-19) and concomitant type 2 diabetes mellitus.Materials and methods. We examined 60 patients with moderate and severe coronavirus disease (COVID-19): Group I (main) – 30 patients with coronavirus disease (COVID-19) with concomitant type 2 diabetes mellitus, mean age (66.70±1.47) years; Group II (comparison group) – 30 patients with coronavirus disease (COVID-19) without diabetes mellitus, mean age (60.13±2.21) years; control group (practically healthy) - 20 people. Nail capillaroscopy, determination of endothelin-1, D-dimer levels, and coagulation parameters were performed on the 2-3rd day after admission to the hospital.Results. In patients of both groups, a significant increase in the level of D-dimer, endothelin-1 was observed. The average values of D-dimer in patients of group I significantly exceeded the average values in patients of group II: 1543.20±254.01 ng FEU/ml and 522.74±39.45 ng FEU/ml respectively (p<0.001). During capillaroscopy in patients of group I, a high frequency of capillary branching (25.8%), bushy capillaries (25.8%) and tortuosity of capillaries (63.3%) was found, microthrombosis and microbleeding were observed more often. In patients of group II, the most common abnormalities were pericapillary edema (83.3%), capillary dilatation (36.7%), dilated and tortuous capillaries (13.3%), and hemosiderin deposits (36.7%). Conclusions. In patients with coronavirus disease (COVID-19) with concomitant type 2 diabetes mellitus, the levels of D-dimer and endothelin-1 indicated a more pronounced endothelial dysfunction. Changes in nail capillaroscopy indicating microvascular damage associated with COVID-19 were more pronounced in patients with concomitant type 2 diabetes.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"172 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72792523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PREVALENCE OF POLYMORPHISM OF THE IL1RL1 GENE (rs950880) AMONG RESIDENTS OF THE PODILSK REGION OF UKRAINE","authors":"D.A. Bagriy, V. Zhebel","doi":"10.24061/2413-0737.27.1.105.2023.1","DOIUrl":"https://doi.org/10.24061/2413-0737.27.1.105.2023.1","url":null,"abstract":"The study of the \"genetic architecture\" of essential hypertension (EH) is becoming increasingly relevant, in particular, the study of the genetic component of the production of peptides, which are used as biomarkers in cardiology. Soluble ST2 of the interleukin-1 receptor family is a promising marker of myocardial remodeling. The genetic basis of ST2 production - SNP rs950880 of the IL1RL1 gene requires a detailed analysis. The aim of the study was to study the prevalence of SNR rs950880 polymorphic variants of the IL1RL1 gene among men living in the Podilsk region of Ukraine with essential hypertension (EH) of varying severity. Material and methods. In the course of the work, 170 men aged 40 to 60 years were examined: 70 without cardiovascular diseases and 100 patients with EH of varying severity (50 with asymptomatic EH (AEH) and 50 patients with EG complicated by CHF II A stage (EH+CHF)). Determination of polymorphism rs950880 of IL1RL1 gene was performed using polymerase chain reaction (PCR). The statistical analysis of the research results included the calculation of the odds ratio, the analysis of conjugation tables. Results. It was established that among residents of the Podilsk region of Ukraine, the SNP variant rs950880 of the IL1RL1 gene, which has the C allele, is the most common: among men without cardiovascular diseases, carriers of the CC and AC variants dominate (45.72% and 42.86%, respectively), the prevalence is similar SNP rs950880 and among patients with EH of different severity (among patients with EH homozygous CC 42.00%, heterozygous AC - 46.00%; in the group EH+CHF - 38.00% and 48.00%, respectively). The AA variant is the least common in all groups (11.42% in the control group, 12.00% among patients with AEH, 14.00% in the EH+CHF group). Conclusions. According to the frequency distribution of the rs950880 polymorphism of the IL1RL1 gene, men with EH of different severity do not reliably differ from the general population of residents of the Podilsk region. Carriership of the investigated polymorphism is not associated with an increased risk of developing EH or complications of CHF. Variability is observed in the frequency distribution of SNR rs950880 IL1RL1 gene alleles in different populations.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86910625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"USE OF SURGICAL METHODS OF TREATMENT FOR THROMBOSIS OF THE INFERIOR VENA CAVA SYSTEM","authors":"Y. Khrebtiy, G. I. Khrebtiy","doi":"10.24061/2413-0737.27.1.105.2023.8","DOIUrl":"https://doi.org/10.24061/2413-0737.27.1.105.2023.8","url":null,"abstract":"Objective. Develop surgical tactics in the treatment of acute deep venous thrombosis (DVT) of the inferior vena cava system.Materials and methods. We analyzed the results of the surgical treatment of 181 patients with DVT for the period from 2001 to 2014. Among the patients with DVT in our study, 37 (20.4%) patients had thrombosis of the inferior vena cava (IVC). Separately, we selected a group of 44 patients with floating DVT. During the treatment, catheter-directed thrombolysis (CDT) was used in 60 (33.1%) patients, systemic thrombolysis in 30 (16.5%), thrombectomy was performed in 44 (24.3%) patients, and anticoagulants were used in 47 (25.9%) patients. Catheter-directed thrombolysis was performed according to the developed method in 30 patients. Results. In the general assessment of the efficiency of the methods, we found that the efficiency of the patented method is 20.5% greater than the efficiency of thrombolysis in the back vein of the foot and 39% greater than the efficiency of systemic thrombolysis (p<0.001). In our study, out of 60 cases of CDT, hemorrhagic complications occurred in 2 (3.3%) cases. In the treatment of patients with floating DVT, we used active surgical tactics when the length of the floating part was more than 4 cm. In the treatment of occlusive thrombosis of the IVC, catheter-directed thrombolysis was used in 9 patients, systemic thrombolysis in 4 patients, and thrombolysis for pulmonary embolism (PE) in 1 patient. 1 patient with thrombosis of the vena cava inferior died of massive PE during anticoagulant therapy. The use of combined methods of DVT treatment of the IVC system made it possible to obtain good and satisfactory results in the immediate period in 166 (92%) patients and in the long-term period in 90 (49.7%) patients.Conclusions. Treatment tactics for thrombosis of IVC system requires an individual approach in each specific case, taking into account the etiological factors of the disease, anatomical features of the structure of IVC system, terms from the onset of the disease, the risk of pulmonary embolism (PE), the presence of concomitant pathology.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78963976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TELEMEDICINE AS A TOOL FOR OPTIMIZING AND IMPROVING METHODS OF PROVIDING MEDICAL AID TO THE POPULATION","authors":"M. Kotsarenko, O. Adamovych, O.P. Adamovych","doi":"10.24061/2413-0737.27.1.105.2023.13","DOIUrl":"https://doi.org/10.24061/2413-0737.27.1.105.2023.13","url":null,"abstract":"Today, the population of Ukraine is experiencing significant difficulties in various spheres of life, however, one of the most urgent problems remains to ensure the proper level of health and preserve the life of the nation. Therefore, the quality of medical care needs special attention now, not only as a direct function but also as a kind of criterion of the level of activity of the health care system and state policy as a whole. One of the innovative directions of the provision of medical care to the population in the 21st century is digital, as well as information and telecommunication technologies, the use of which made it possible to create a single medical information space that combines practical and theoretical medicine, ensures the planning of new scientific research and targeted training specialists of various fields of medicine, who later implement knowledge and skills in medical practice. The purpose of our research was to study the directions of using telemedicine technologies in the work of medical institutions and the possibilities of improving the provision of medical care to the population through their use.Research methods. The used methods of the system approach and system analysis made it possible to investigate the possibilities of optimal use of telemedicine technologies and analyze existing problems and propose ways to solve them.The results. Modern sources of professional literature consider telemedicine from different angles: as a set of tools and complexes that provide users with access to current medical resources, including international ones, realize the potential of modern information and telecommunication technologies in health care; as well as a multi-level and multi-component system, which includes both medical organizations with their professional, informational and educational resources, medical diagnostic devices, databases and system users, as well as technical means of access to telecommunication networks, communication channels and network means, transmitters and other converters of medical information into digital electrical signals for further transmission through communication channels. Taking into account the multidisciplinary nature of medicine and a wide range of medical services, the unification of clinical, anamnestic, laboratory, radiation, etc. databases and the possibility of their transmission and reception using equipment from different manufacturers is of particular importance. One of the unified and most widely used standards in the field of medical image transmission today is DICOM (Digital Imaging and Communications in Medicine) - an international standard for processing and transmitting radiological and other medical images from medical devices to a computer, which has found wide application in various industries of medicineConclusions. The analysis of literary sources proves the need for further detailed study of the possibilities of digital and information technologies in medicine to impr","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81092423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of antimicrobial activity of dimethylhydrazones","authors":"A. Humenna, V. V. Listvan, V. Listvan, N. Yakovychuk, O. Blinder, D. Rotar","doi":"10.24061/2413-0737.xxvi.2.102.2022.3","DOIUrl":"https://doi.org/10.24061/2413-0737.xxvi.2.102.2022.3","url":null,"abstract":"Modern methods of treatment and prevention of infectious diseases require the administration of antibiotics and antiseptics. Excessive and uncontrolled use leads to the selection of antibiotic-resistant strains of microorganisms, which dictates the need to find new alternative antimicrobial drugs. We searched for and studied the antimicrobial activity of new dimethylhydrazones. The compounds we studied showed significant antimicrobial activity, especially against gram-positive bacteria, S.aureus ATCC 25923 and lower antimicrobial activity against gramnegative bacteria, indicating the viability of these compounds and the need for more in-depth study of its antimicrobial activity on a wide range of microorganisms.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73083496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advantages of simulative training in practice of practical skills and manipulations of future doctors","authors":"I. Kozlovska, O. Kolotylo, Y. Kulachek, O. Rusak, U. Marusyk, V. Smandych","doi":"10.24061/2413-0737.xxvi.2.102.2022.15","DOIUrl":"https://doi.org/10.24061/2413-0737.xxvi.2.102.2022.15","url":null,"abstract":"Relevance. The problem of qualitative training of future doctors is an extremely important issue throughout the world and in Ukraine, including. To improve their practical skills and medical manipulations, they must be brought to ideal and automatism, which is possible due to modern methods of training in the conditions of the simulation center, due to the development of skills on standardized patients and simulators.Objective. To compare the quality of education and working out practical skills, manipulations and evaluate the efficiency of training using patients of medical institutions and mannequins or standardized patients in the center of simulation medicine.Material and methods. We compared the efficiency of working out practical skills, medical manipulations and ability to qualitatively apply them in practice, especially in providing emergency medical care to students of 5 and 6 courses.Results. It is not always possible to immediately establish direct contact with the patient, as opposed to a standardized patient, which extremely accurately demonstrates all complaints and positive symptoms that will allow to confirm the diagnosis. Students are not comfortable with the patient in the clinic or afraid to harm the patient and refuse to survey or conduct a certain skill, while on a mannequin or standardized patient without any fear, a practical skill can be repeated with the required number of times. At a standardized patient or virtual mannequin, a teacher can stop a student at any stage of execution, indicate an error or once again explain the technique of manipulation, and a student may repeat a few times, bringing it to full automatism. Another extremely important advantage of simulating learning is the opportunity at the requested moment, and not when there is a suitable patient in the clinic, to create, simulate and beat the desired situation. This is especially important when working out emergency care skills in conditions that are menacing. \u0000Conclusion. The medical education process must be effective and secure for all participants, specially patients, which is possible only due to simulation centers. Of course, the development of practical skills and manipulations in the simulating center allows to improve traditional types of education, pay more attention to handling skills, allowing, realizing and correcting mistakes, analyze the situation and draw conclusions.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79266403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and electroneuromyographic peculiarities of spastic syndrome in children with organic lesions of the nervous system","authors":"I. A. Zorii, N. Vasylieva, I. Krychun, O. M. Nicka, I.B. Kharmanska","doi":"10.24061/2413-0737.xxvi.2.102.2022.11","DOIUrl":"https://doi.org/10.24061/2413-0737.xxvi.2.102.2022.11","url":null,"abstract":"Topicality. Organic lesions of the central nervous system (CNS) are a group of neuropsychiatric disorders caused by various pathological factors affecting the brain. Infantile cerebral palsy (ICP) is the most common neurological disease diagnosed in children at an early age. 80 % of children with ICP suffer from spastic forms, the main symptom of which is an abnormal increase in muscle tone – spasticity. This syndrome can be objectified by electroneuromyographic (ENMG) examination which enables qualitative and quantitative assessments of the nervousmuscular system state.Objective: to determine clinical and electroneuromyographic peculiarities of the spastic syndrome of ICP children depending on the intensity of motor disorders.Materials and methods. 122 ICP children were examined (an average age 8,8 ± 3,7 years) and distributed into groups by the results of Gross Motor Function Classification Expanded & Revised (GMFCS E&R). All the patients underwent careful neurological examination and ENMG examination. To assess suprasegmental and segmental levels of nervous system lesions, the parameters of H-reflex and F-wave were analyzed.Results. Spastic forms of ICP were diagnosed in the majority of the examined children. Orthopedic pathology was found more often among ICP children with marked motor disorders, including equinovalgus position and planovalgus foot deformity. According to ENMG parameters ICP patients presented conduction disorders manifested by increased amplitude of М response, especially in testing the tibial nerve, increased Нmах/Мmах ratio and the amplitudes of Н-reflex and F-wave.Conclusions. According to the results of the conducted study, ENMG parameters changed on the side of deterioration depending on the degree of motor activity disorders by Gross Motor Function Classification.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"41 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79436578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial cerebral dysfunction in rats with scopolamine-induced neurodegeneration under enalapril effect","authors":"O. Kmet, N. Filipets, T. Kmet, N.Y. Andriychuk, D. Tymkul","doi":"10.24061/2413-0737.xxvi.2.102.2022.10","DOIUrl":"https://doi.org/10.24061/2413-0737.xxvi.2.102.2022.10","url":null,"abstract":"Objective. Neurodestructive diseases are characterized by complex pathobiochemical cascades in the neuron, which cause disturbances in energy metabolism and the formation of mitochondrial dysfunction. The renin-angiotensin system plays an important role in the physiological functioning of mitochondria, the excessive activity of which increases the risk of neurodegenerative diseases of the brain. Although angiotensin-converting enzyme inhibitors are now considered as means of prevention and treatment of ischemic lesions of the central nervous system, their corrective properties in the development of central neurodegeneration continue to be refined. The objective of our study was investigation of enalapril effect, as an angiotensin-converting enzyme inhibitor, in case of mitochondrial dysfunction of the cerebral cortex and hippocampus of rats under conditions of scopolamine-induced neurodegeneration reproducing development of Alzheimer’s disease in the experiment.Material and methods. Scopolamine hydrochloride (Sigma, USA) was injected in rats through the peritoneum at a dose of 1 mg/kg for 27 days to simulate Alzheimer’s disease. Starting from the 28th day of the experiment, enalapril was introduced through the peritoneum at a dose of 1 mg/kg, once a day for 14 days. \u0000Results. Under conditions of scopolamine-induced Alzheimer’s disease in the mitochondrial fraction of the cerebral cortex and hippocampus of rats free radical oxidation of lipids and proteins ncreases, and activity of Krebs cycle enzymes decreases – α-ketoglutarate dehydrogenase and succinate dehydrogenase; light dispersion decreases and a relative rate of mitochondrial swelling increases. After enalapril administration for 14 days to rats with scopolamine-induced Alzheimer’s disease the content of products reacting with 2-thiobarbituric acid and protein oxidation modification decreases in the mitochondrial fraction of the cerebral cortex and hippocampus; in both examined structures, the activity of catalase, αketoglutarate dehydrogenase, succinate dehydrogenase increases, and superoxide dismutase – only in the cerebral cortex; a gradual decrease of light dispersion and relative rate of mitochondrial swelling occurs.Conclusion. Improvement of the antioxidant system state and energy supply of mitochondria, decreased intensity of mitochondrial swelling in the cerebral cortex and hippocampus of rats with scopolamine-induced Alzheimer’s disease are indicative of the protective properties of enalapril.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87863414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}