Journal of stem cell therapy and transplantation最新文献

{"title":"The outcome of autologous hematopoietic stem cell transplantation in patients with multiple myeloma. The experience of King Fahad Specialist Hospital in Dammam, Saudi Arabia","authors":"Al-Anazi Khalid Ahmed, E. Mutahar, O. Abduljalil, S. Kanfer, P. Kaloyannidis, A. Estanislao, I. Apostolidis, N. Almokhtar, M. Darweesh, M. Abdulbaqi, W. Alenazi, Z. Alshammasi, Z. Alshaibani, M. Kawarie, H. Raslan, A. AlBahrani, A. Alsaber, N. Almulhem, W. Dridi, A. Aldayel, R. Alrabeh, A. Alshami, A. Ayyad, F. Abu Rahma, J. Lardizabal, A. Salam, K. Haque, A. AlSagheir, H. Alhashmi","doi":"10.29328/journal.jsctt.1001027","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001027","url":null,"abstract":"Background: Aautologous hematopoietic stem cell transplants (HSCT) is the standard of care for newly diagnosed patients with multiple myeloma (MM) who are eligible for autologous transplantation. Although cryopreservation is routinely employed, autologous HSCT can be performed using non-cryopreserved stem cells. Methods and materials: A retrospective study of patients with MM who received autologous HSCT between the 10th of October 2010 and the 31st of January 2022 at King Fahad Specialist Hospital (KFSH) in Dammam, Saudi Arabia was performed. Results: Over 11 years and 113 days, a total of 135 autologous HSCTs were performed for 119 patients with MM at our institution. Single autologous HSCTs were performed for 119 patients, while 16 of these patients received either planned tandem autologous transplants or second autografts due to either progression or relapse of their myeloma. The median age of patients with MM at autologous HSCT was 51.5 years. At presentation of their MM, the following high-risk (HR) features were encountered: stage III disease according to the revised international scoring system (RISS) in 12.3%; adverse cytogenetics in 31.93% of patients; advanced bone disease in 60.50%; and renal dysfunction or failure in 11.76% of patients. A total of 104 autologous HSCTs (77.04%) were performed without cryopreservation while 31 autografts (22.96%) were performed using cryopreserved apheresis stem cell products. Additionally, 54 autologous HSCTs (40.00%) were done at outpatient while 81 autografts (60.00%) were performed in an inpatient setting. Survival for 100 days post-HSCT for all patients with MM who received autologous transplants including those done at outpatient was 100%. The 4 years overall survival (OS) an progression-free survival (PFS) for patients with MM who received non- cryopreserved or fresh autologous HSCTs were 82% and 68% respectively. Conclusion: Autologous HSCT without cryopreservation is safe, and feasible and can lead to short-term as well as long-term outcomes that are comparable to autologous transplantation with cryopreservation. Non- cryopreserved autologous grafts allow the performance of autologous transplants in an outpatient setting to save beds and reduce costs.","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41822509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of collagenase to isolate adipose tissue-derived stem cells – substantial manipulation or not?","authors":"E. Koellensperger, F. Gramley, G. Germann, U. Leimer","doi":"10.29328/journal.jsctt.1001026","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001026","url":null,"abstract":"Background: Collagenase is commonly used to isolate the stromal vascular fraction (SVF) or adipose tissue-derived stem cells (ADSCs) from human adipose tissue. Enzymatic breakdowns may be a substantial manipulation according to the classifications of medical regulatory authorities. This study investigates the possible effects of human adipose tissue dissociation with collagenase on in vitro function and behavior of ADSCs. Methods and results: Adipose tissue from nine donors was divided into two equal fractions. SVF was then isolated either mechanically or with collagenase, respectively. The resulting cells were analyzed for their surface markers directly after isolation and at passage five. Proliferation, tri-lineage differentiation, and secretome markers were measured after passage four. Using collagenase compared to mechanical isolation did not alter the expression of typical surface markers of ADSCs. ADSCs isolated with collagenase showed a significantly shorter population doubling time (p < 0.001), a significantly higher mean specific GPDH-activity, a stronger intensity in perilipin staining (p = 0.005), and a significantly higher extracellular calcium deposition (p = 0.006) than mechanically isolated ADSCs. The expression of adipogenic and osteogenic marker genes was not different in mechanically versus enzymatically isolated ADSCs. There were no significant differences in proteoglcyan production (p > 0.05) and the concentration of type 2 collagen. Except for an increased CCL2 concentration in mechanically isolated ASDCs (p = 0.01), there were no significant differences in the concentration of secreted proteins between both isolation methods. Conclusions: The use of collagenase does not substantially impair central in vitro characteristics and functions of human adipose tissue-derived stem cells.","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44571239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allogeneic hematopoietic cell transplantation to treat two synchronous hematologic malignancies","authors":"Santi Enrico, Cerretti Raffaella, De Angelis Gottardo, Mariotti Benedetta, Ciangola Giulia, Page Camilla, Rossi Elisa Lindfors, Pasqualone Gianmario, A. William","doi":"10.29328/journal.jsctt.1001025","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001025","url":null,"abstract":"Allogeneic hematopoietic cell transplantation often represents the only solution for several poor-prognosis hematologic malignancies. The curative strategy for patients with synchronous hematologic disorders is always difficult and, in most cases, ineffective. Herein, we report an unusual case of synchronous hematologic disorders successfully treated with an “ad-hoc” conditioning regimen followed by allogeneic hematopoietic cell transplantation.","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44470208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-related changes in cell yield and viability of feline Adipose Tissue-Derived Mesenchymal Stem (fAD-MSCs)","authors":"Nissar Shazia, R. Geetha, Basha Sabiha Hayath, Kannan Ta, Hussain Shahid","doi":"10.29328/journal.jsctt.1001024","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001024","url":null,"abstract":"In the present study, omental adipose tissue was collected from, the animals that underwent ovariectomy and ovariohysterectomy, surgical procedures, at the age of seven months to 11/2 years of age groups. The sample was digested with 0.1% (W/V) collagenase type I and transferred to a beaker with a magnetic stirrer and kept in a stirrer with 600 rpm at 37 °C for 30 minutes. The viability of the cell was evaluated by the trypan blue exclusion method using a hemocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the member in a dead cell and dye it blue. In the present study, the cell yield of fAD-MSCs was 8.15 ± 0.68, 6.55 ± 0.26, 4.85 ± 0.42, 3.90 ± 0.34, and 3.51 ± 0.43 in different age groups viz., 7,8,9 month 1 and 1½ year respectively. In younger age groups, cell yield and viability percentage were more than in animals of higher age groups. In the younger age group, stem cells proliferation status is considered potent for therapeutic application.","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45918776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human mesenchymal stem cells conditioned media promotes the wound healing process - An in vitro study","authors":"R. K. Jain, D. Vakil, Corey Cunningham, K. Sidhu","doi":"10.29328/journal.jsctt.1001016","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001016","url":null,"abstract":"","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44771546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rising role of natural killer cells in patients with malignant hematological disorders and in recipients of hematopoietic stem cell transplantation","authors":"K. Al-Anazi","doi":"10.29328/journal.jsctt.1001015","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001015","url":null,"abstract":"Natural killer (NK) cells, the third population of lymphoid cells, comprise 5%-25% of peripheral blood (PB) lymphocytes and represent the irst line of defense against infections and tumors [1-7]. They can be derived from: bone marrow, PB, cryopreserved umbilical cord blood (UCB), human embryonic stem cells (hESCs), induced pluripotent stem cells (iPSCs), and various cell lines such as NK-92 and KHYG-1 [1]. NK cells; which have been divided into cytotoxic, tolerant, and regulatory subsets; are classi ied into: (1) naïve CD56 bright CD 16 dim CD 3 dim cells, (2) mature CD56 dim CD16 bright CD3 dim cells, and (3) lymphoid tissue-resident CD69+/CXCR6+ NK cells [1,2,8-11]. Although NK cells have been traditionally considered as part of the innate immune system, they have recently been shown to exhibit many of the features associated with adaptive immunity [8,12]. The functions of NK cells which are in luenced by several cytokines include: elimination of infected cells, destruction of cancer cells, reducing the incidence of graft versus host disease (GVHD) following hematopoietic stem cell transplantation (HSCT), and regulation of pregnancy outcome [10,11,13]. NK cell function is inely tuned by activating and inhibitory receptors that recognize both foreign and self-antigens expressed by NK cell-susceptible targets [7,14]. Activated NK cells interact with dendritic cells (DCs) and mesenchymal stem cells (MSCs) and the complicated crosstalks between NK cells, MSCs, and DCs may alter the functions of any of the 3 cell types [15-27].","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49370630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the use of GABAergic interneurons for the treatment of epilepsy","authors":"Federica Frisina, G. Valetti, Giulia Zuccarini, L. Conti, G. Merlo","doi":"10.29328/journal.jsctt.1001014","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001014","url":null,"abstract":"Abbreviations: 5HT3aR: serotonin Receptor 3a; AED: Anti-Epileptic Drug; CB: Calbindin; CGE: Caudal Ganglionic Eminence; CNS: Central Nervous System; CR: Calretinin; DCX: Doublecortin; ESC: Embryonic Stem Cells; fEPSP: fi eld Excitatory Postsynaptic Potential; GABA: Gamma-AminoButirric Acid; GFAP: Glial Fibrillary Acidic Protein; HFO: High Frequency Oscillation; IN: Inhibitory Neurons or InterNeuron; iPSC: induced Pluripotent Stem Cells; KA: Kainic Acid; LGE: Lateral Ganglionic Eminence; MES: Maximal Electroshock; MGE: Median Ganglionic Eminence; NeuN: Neuronal Nuclear Antigen; nNOS: Neuronal Nitric Oxide Synthase; NPY: Neuropeptide Y; NSC: Neural Stem Cells; PTZ: Pentylenetetrazole; PV: Parvalbumin; SE: Status Epilepticus; SRS: Spontaneous Recurrent Seizures; SST: Somatostatin; TLE: Temporal Lobe Epilepsy; VIP: Vasoactive Intestinal Peptide Introduction","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49201240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of autologous adipose-derived stem cells for thin endometrium treatment in patients with failed ART programs","authors":"I. Sudoma, L. Pylyp, Y. Kremens’ka, Y. Goncharova","doi":"10.29328/journal.jsctt.1001013","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001013","url":null,"abstract":"","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48340043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Report on the Effect of Mesenchymal Stem Cell Therapy in Patients with Chronic Lung Allograft Dysfunction","authors":"C. Keller, B. V. Wie, Athena L. Russell, D. O. Hodge, D. Erasmus, A. Zubair","doi":"10.29328/JOURNAL.JSCTT.1001012","DOIUrl":"https://doi.org/10.29328/JOURNAL.JSCTT.1001012","url":null,"abstract":"Lung transplantation is the best option of care for a variety of end-stage lung conditions no longer responding to standard options of medical or surgical care [1]. The most common causes of early morbidity and mortality after transplant include primary graft dysfunction [2], acute infectious complications [3], acute cellular rejection [4], or acute antibody-mediated rejection [5]. Long-term survival of lung transplant recipients is compromised by chronic lung allograft dysfunction (CLAD) [6], either obstructive (or bronchiolitis obliterans syndrome [BOS]) [7] or restrictive (restrictive allograft syndrome) [8].","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48666884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A flow perfusion bioreactor with controlled mechanical stimulation: Application in cartilage tissue engineering and beyond","authors":"BJ Van Wie","doi":"10.29328/journal.jsctt.1001011","DOIUrl":"https://doi.org/10.29328/journal.jsctt.1001011","url":null,"abstract":"Articular cartilage (AC) is a semitransparent, strong connective tissue that covers the articulating surfaces of bones [1]. It contains a rubbery matrix with extensive amounts of water, collagen, and proteoglycan ibers [2], and its unique structure allows it to absorb mechanical loads while creating a near-frictionless environment in articulating joints [3]. However, because AC is avascular and aneural, it has poor intrinsic ability for self-repair after injury [4]. Damage to AC is progressive and leads to osteoarthritis, a condition that affects 5 percent of the general population and 70 percent of those over age of 65, and costs nearly $8 billion annually in health care expenses [5]. Due to the socioeconomic and human impacts of this condition there has been a concerted effort to develop innovative strategies to repair damaged AC. One promising approach is the use of grafts comprised of replacement AC tissue, engineered to have properties similar to endogenous AC.","PeriodicalId":92683,"journal":{"name":"Journal of stem cell therapy and transplantation","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69947127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}