Journal of glycobiology最新文献_第9页

Preclinical Evaluation of Symmetrical Diiodinated Squaraine Dye on Experimental Animal Models 对称二碘化方碱染料在实验动物模型上的临床前评价

Journal of glycobiology Pub Date : 2013-05-28 DOI: 10.4172/2168-958X.S1-003

Soumya Ms

{"title":"Preclinical Evaluation of Symmetrical Diiodinated Squaraine Dye on Experimental Animal Models","authors":"Soumya Ms","doi":"10.4172/2168-958X.S1-003","DOIUrl":"https://doi.org/10.4172/2168-958X.S1-003","url":null,"abstract":"Photodynamic therapy involves administration of a tumor localizing photosensitizing agent, followed by the activation of the agent by light of a specific wavelength. This therapy results in a sequence of photochemical and photobiological processes that cause irreversible photo damage to the tumor tissue. Only a few photosensitizers have received official approval around the world. But they are with some adverse side effects. Because of these draw backs search for new photosensitizers is in progress. The dye selected in our study- Symmetric diiodinated squaraine- is one of these newly developed photosensitizers. We have examined the in vivo distribution of the dye on normal and skin tumor induced animal models to check the retention time of the dye. The mechanism of action of photosensitizers in selective killing of cancer cells involves the generation of oxidative stress whereby the malignant cells are damaged, in presence of light of appropriate wavelength. Thus we also intended to study whether there is the initiation of oxidative damage in the normal tissues in the body of dye injected mice in the absence of light. The results show that maximum amount of the dye was accumulated in the different organs of the body 4 h after the administration. The compound is tumor specific since after 24 h of injection, it was retained only in the tumor site and in the immediate surroundings of the tumor. This shows the selectivity of the dye to tumor tissue. The administration of the squaraine dye in the system induces no significant change in the level of lipid peroxidation products and activity of antioxidant enzymes when compared to the control mice. This indicates that the squaraine dye does not induce any oxidative stress to the normal tissues in the body and hence the dye can be safely used for photodynamic therapy.","PeriodicalId":92404,"journal":{"name":"Journal of glycobiology","volume":"2013 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2168-958X.S1-003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70862921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Pseudomyxoma peritonei: Uninvited goblet cells, ectopic MUC2 腹膜假性粘液瘤:未邀请的杯状细胞，异位MUC2

Journal of glycobiology Pub Date : 2013-05-27 DOI: 10.4172/2168-958X.S1-002

A. Amini, Samar Masoumi Moghaddam, A. Ehteda

引用次数: 1

Rhizoctonia Bataticola Lectin (RBL) Induces Apoptosis in Human Ovarian Cancer PA-1 Cells and Suppresses Tumor Growth In Vivo Bataticola Rhizoctonia凝集素(RBL)诱导人卵巢癌PA-1细胞凋亡并抑制肿瘤生长的实验研究

Journal of glycobiology Pub Date : 2013-03-21 DOI: 10.4172/2168-958X.S1-001

Sachin M. Eligar, R. Pujari, Mohammed Azharuddin Savanur, Nagaraja N. Nagre, Srikanth Barkeer, A. Ingle, R. Kalraiya, Bale M. Swamy, P. Shastry, S. Inamdar

{"title":"Rhizoctonia Bataticola Lectin (RBL) Induces Apoptosis in Human Ovarian Cancer PA-1 Cells and Suppresses Tumor Growth In Vivo","authors":"Sachin M. Eligar, R. Pujari, Mohammed Azharuddin Savanur, Nagaraja N. Nagre, Srikanth Barkeer, A. Ingle, R. Kalraiya, Bale M. Swamy, P. Shastry, S. Inamdar","doi":"10.4172/2168-958X.S1-001","DOIUrl":"https://doi.org/10.4172/2168-958X.S1-001","url":null,"abstract":"Background: A lectin from the plant pathogenic fungus Rhizoctonia bataticola (RBL) has shown unique sugarbinding specificity towards the complex N-glycans, which are known to be expressed in many cancers, including ovarian cancer. Previous studies have demonstrated that RBL exerts cytotoxic effects on human ovarian cancer PA-1 cells. In the present study, we determined the signaling mechanism of RBL-induced cytotoxicity in PA-1 cells upon binding to cell surface receptors and also assessed the anti-tumor activity of RBL in NOD-SCID mice bearing ovarian xenografts. Methods: Cytotoxicity was determined by MTT assay, Cell cycle analysis, Annexin V staining, and expression of caspase-3 was determined by flow cytometry using specific fluorescent detection reagents. Western blotting was carried out to observe the intermediates of apoptotic pathway. Results: Treatment of PA-1 cells with RBL decreased their viability in a dose- and time- dependent manner. Cell cycle analysis revealed an increase in the hypo diploid population in response to lectin treatment. RBL-treated cells stained with Annexin V and PI showed an increase in both the early and late apoptotic populations. The mechanism of RBL-induced apoptosis in PA-1 cells was assessed by the observed decrease in MMP (ΔΨm), release of cytochrome-c, activation of caspase-9 and -3, and cleavage of PARP. Specific pharmacological inhibitors revealed the involvement of caspase-9 and -3 but not caspase-8. RBL at a subtoxic concentration (50 μg/mouse) suppressed tumor growth in NOD-SCID mice bearing PA-1 xenografts. Conclusions: The present study demonstrates that, RBL induced cytotoxicity in PA-1 cells is mediated by intrinsic apoptotic pathway. The present study strengthens the merit of RBL for its potential application in ovarian cancer research.","PeriodicalId":92404,"journal":{"name":"Journal of glycobiology","volume":"2013 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70862668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Controversial Sialoglycosphingolipids Functions in Tumor Biology 有争议的涎糖鞘脂在肿瘤生物学中的功能

Journal of glycobiology Pub Date : 2013-01-01 DOI: 10.4172/2168-958X.1000E107

Cheorl-Ho Kim

引用次数: 1

Development of a Polyclonal Antibody Against Synthetic Human Immunoglobulin A1 Hinge Glycopeptide 抗合成人免疫球蛋白A1铰链糖肽多克隆抗体的制备

Journal of glycobiology Pub Date : 2012-12-31 DOI: 10.4172/2168-958X.1000103

Koichiro Yamamoto, Hideo Hori, Yoshihiro Yamamoto, Kazuo Takahashi, Y. Yuzawa, Y. Hiki

{"title":"Development of a Polyclonal Antibody Against Synthetic Human Immunoglobulin A1 Hinge Glycopeptide","authors":"Koichiro Yamamoto, Hideo Hori, Yoshihiro Yamamoto, Kazuo Takahashi, Y. Yuzawa, Y. Hiki","doi":"10.4172/2168-958X.1000103","DOIUrl":"https://doi.org/10.4172/2168-958X.1000103","url":null,"abstract":"Background: Undergalactosylated IgA1 has been found to be increased in IgA nephropathy (IgAN) by an ELISA assay using Helix aspersa agglutinin (HAA) that recognizes N-acetylgalactosamine (GalNAc). In this study, we developed a polyclonal antibody (anti-sHGP antibody) against a synthetic IgA1 hinge peptide with five GalNAc residues. \u0000Methods: The specificity of the anti-sHGP antibody was evaluated through the incremental treatment of IgA with corresponding glycosidases. Then, the susceptibility of the IgA to anti-sHGP antibody was compared among IgAN patients (n=39), patients with other forms of kidney diseases (OKD, n= 36) and healthy controls (n=37), using ELISA assay. The association of the binding abilities between anti-sHGP antibody and HAA were evaluated blindly using same 85 sera. \u0000Results: The binding ability of the anti-sHGP antibody was increased relative to the incremental treatments of neuraminidase (desialo-IgA), galactosidase (desialo/degalacto IgA). The binding levels of anti-sHGP antibody against serum IgA were significantly higher in IgAN patients compared to both healthy controls (P=0.008) and those with OKD (P=0.049). The binding levels of anti-sHGP antibody were closely related to those of HAA ELISA in the same patient sera (RR2=0.5964). \u0000Conclusions: It was certified that the anti-sHGP antibody recognized GalNAc residues in the hinge peptide of human IgA1 as well as HAA. The increased antigenicity of IgA against the antibody in IgAN suggested that a serum IgA1 exposing GalNAc residue was increased in IgAN. It would be necessary to identify the precise structure of O-glycans specific to IgAN for developing a more specific antibody.","PeriodicalId":92404,"journal":{"name":"Journal of glycobiology","volume":"2012 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2012-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70858613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Time for Molecular Glycobiology 分子糖生物学时间到了

Journal of glycobiology Pub Date : 2012-07-14 DOI: 10.4172/2168-958X.1000E106

Zhengliang L. Wu

引用次数: 4

The Name of the – ose: An Editorial on Carbohydrate Nomenclature 糖的名称:碳水化合物命名法评论

Journal of glycobiology Pub Date : 2012-06-17 DOI: 10.4172/2168-958X.1000E105

N. Price

引用次数: 1

Heparin as an Immunosuppressant: Therapeutic Potential beyond Anticoagulation 肝素作为一种免疫抑制剂:抗凝血以外的治疗潜力

Journal of glycobiology Pub Date : 2012-05-22 DOI: 10.4172/2168-958X.1000E104

Simi Ali, J. Kirby

引用次数: 1

Fucanome and Galactanome: Marine Glycomics Contribution 岩藻烯和半乳糖烯:海洋糖组学贡献

Journal of glycobiology Pub Date : 2012-01-31 DOI: 10.4172/2168-958X.1000101

V. Pomin

{"title":"Fucanome and Galactanome: Marine Glycomics Contribution","authors":"V. Pomin","doi":"10.4172/2168-958X.1000101","DOIUrl":"https://doi.org/10.4172/2168-958X.1000101","url":null,"abstract":"Following the genome, transcriptome and proteome, the glycome has currently launched in biology bringing apparently more challenges than the first projects. The long major conception of carbohydrates as just energetically involved class of biomolecules has fallen apart as innumerable essential biological actions have been raised, impossible to be enlisted all together at once. Such actions involve not only vital roles in cell and physiology but within a different context they also include potent therapeutic actions in coagulation, thrombosis, inflammation, virosis, pathogenesis, tumorigenesis, metastasis and angiogenesis. Glycomics is so extensive project that subdivision is necessary for its progress. Specific segments of research have been created about particular bioactive sugar classes such as sialome for sialic acids, and heparanomics for heparan bioactive domains. Fucanome and galactanome must be also included in the recent glycomics age with respect to the relatively new class of marine fucose- or galactose-composing polysaccharides named sulfated fucans and sulfated galactans. These glycans are very biologically relevant since they show potent pharmacological properties in many of the above-mentioned systems; besides in sea-urchins they are responsible to control a very rare case of carbohydrate-mediated cell signaling event, the acrosome reaction. The structure of these glycans is very restricted to phyla or species of occurrence. Among all marine organisms, only invertebrates and red algae have been known so far to express high molecular weight polymers composed of well-defined structures. These structural features are unique and very rare among any bioactive polysaccharide ever studied. This would make fucanome and galactanome differential glycomics subprojects in terms of structure and phylogeny. This paper not only brings out novel segments and their respective contribution to glycomics but also highlights the great relevance of pharmacological effects of these new glycans in the current glycome era.","PeriodicalId":92404,"journal":{"name":"Journal of glycobiology","volume":"1 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2012-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70858542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role of Cell Wall Polysaccharides during Recognition of Candida albicans by the Innate Immune System 细胞壁多糖在先天免疫系统识别白色念珠菌中的作用

Journal of glycobiology Pub Date : 2012-01-02 DOI: 10.4172/2168-958X.1000102

Luis A. Pérez-García, D. F. Díaz-Jiménez, Adolfo López-Esparza, H. Mora-Montes

引用次数: 28