Bone Marrow Research最新文献_第2页

Cigarette Smoking Is Associated with a Lower Concentration of CD105(+) Bone Marrow Progenitor Cells. 吸烟与CD105(+)骨髓祖细胞浓度降低有关。

Bone Marrow Research Pub Date : 2015-01-01 Epub Date: 2015-08-09 DOI: 10.1155/2015/914935

Shaul Beyth, Rami Mosheiff, Ori Safran, Anat Daskal, Meir Liebergall

{"title":"Cigarette Smoking Is Associated with a Lower Concentration of CD105(+) Bone Marrow Progenitor Cells.","authors":"Shaul Beyth, Rami Mosheiff, Ori Safran, Anat Daskal, Meir Liebergall","doi":"10.1155/2015/914935","DOIUrl":"https://doi.org/10.1155/2015/914935","url":null,"abstract":"Cigarette smoking is associated with musculoskeletal degenerative disorders, delayed fracture healing, and nonunion. Bone marrow progenitor cells (BMPCs), known to express CD105, are important in local trophic and immunomodulatory activity and central to musculoskeletal healing/regeneration. We hypothesized that smoking is associated with lower levels of BMPC. Iliac bone marrow samples were collected from individuals aged 18-65 years during the first steps of pelvic surgery, under IRB approval with informed consent. Patients with active infectious or neoplastic disease, a history of cytotoxic or radiation therapy, primary or secondary metabolic bone disease, or bone marrow dysfunction were excluded. Separation process purity and the number of BMPCs recovered were assessed with FACS. BMPC populations in self-reported smokers and nonsmokers were compared using the two-tailed t-test. 13 smokers and 13 nonsmokers of comparable age and gender were included. The average concentration of BMPCs was 3.52 × 10(5)/mL ± 2.45 × 10(5)/mL for nonsmokers versus 1.31 × 10(5)/mL ± 1.61 × 10(5)/mL for smokers (t = 3.2, P = 0.004). This suggests that cigarette smoking is linked to a significant decrease in the concentration of BMPCs, which may contribute to the reduced regenerative capacity of smokers, with implications for musculoskeletal maintenance and repair. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2015 ","pages":"914935"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/914935","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34154540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Comparing Two Types of Rabbit ATG prior to Reduced Intensity Conditioning Allogeneic Hematopoietic SCT for Hematologic Malignancies. 比较两种类型的兔ATG在降低强度条件的同种异体造血SCT治疗血液恶性肿瘤。

Bone Marrow Research Pub Date : 2015-01-01 Epub Date: 2015-03-22 DOI: 10.1155/2015/980924

Sandra Paiano, Eddy Roosnek, Yordanka Tirefort, Monika Nagy-Hulliger, Stavroula Masouridi, Emmanuel Levrat, Michael Bernimoulin, Saadia Huguet, Alessandro Casini, Thomas Matthes, Kaveh Samii, Jakob R Passweg, Yves Chalandon

{"title":"Comparing Two Types of Rabbit ATG prior to Reduced Intensity Conditioning Allogeneic Hematopoietic SCT for Hematologic Malignancies.","authors":"Sandra Paiano, Eddy Roosnek, Yordanka Tirefort, Monika Nagy-Hulliger, Stavroula Masouridi, Emmanuel Levrat, Michael Bernimoulin, Saadia Huguet, Alessandro Casini, Thomas Matthes, Kaveh Samii, Jakob R Passweg, Yves Chalandon","doi":"10.1155/2015/980924","DOIUrl":"https://doi.org/10.1155/2015/980924","url":null,"abstract":"Different rabbit polyclonal antilymphocyte globulins (ATGs) are used in allogeneic hematopoietic stem cell transplantation (alloHSCT) to prevent graft-versus-host disease (GvHD). We compared 2 different ATGs in alloHSCT after reduced intensity conditioning (RIC) for hematological malignancies. We reviewed 30 alloHSCT for hematologic malignancies performed between 2007 and 2010 with fludarabine and i.v. busulfan as conditioning regimen. Patients alternatingly received Thymoglobulin or ATG-F. Median followup was 3.3 (2.5-4.5) years. Adverse events appeared to occur more frequently during Thymoglobulin infusion than during ATG-F infusion but without statistical significance (P = 0.14). There were also no differences in 3-year overall survival (OS), disease-free survival (DFS), relapse incidence, and transplant related mortality (TRM) in the Thymoglobulin versus ATG-F group: 45.7% versus 46.7%, 40% versus 33.7%, 40% versus 33.3%, and 20% versus 33.3%. The same held for graft failure, rejection, infectious complications, immune reconstitution, and acute or chronic GvHD. In patients transplanted for hematologic malignancies after RIC, the use of Thymoglobulin is comparable to that of ATG-F in all the aspects evaluated in the study. However due to the small number of patients in each group we cannot exclude a possible difference that may exist. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2015 ","pages":"980924"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/980924","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33216199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

The Use of Real Time PCR Genotyping to Detect Activating GNAS Mutations in McCune-Albright Syndrome 实时PCR基因分型检测mcune - albright综合征中激活GNAS突变

Bone Marrow Research Pub Date : 2014-03-29 DOI: 10.4172/2161-1041.1000126

Mariani Bmp, E. Trarbach, R. Toledo, A. Lerário, A. Latronico, Mendonça Bb, Fragoso Mcbv

{"title":"The Use of Real Time PCR Genotyping to Detect Activating GNAS Mutations in McCune-Albright Syndrome","authors":"Mariani Bmp, E. Trarbach, R. Toledo, A. Lerário, A. Latronico, Mendonça Bb, Fragoso Mcbv","doi":"10.4172/2161-1041.1000126","DOIUrl":"https://doi.org/10.4172/2161-1041.1000126","url":null,"abstract":"Introduction: The McCune-Albright syndrome (MAS) is a genetic disease clinically characterized by the triad: bone fibrous dysplasia cafe-au-lait skin spots and endocrine hyperfunction, such as precocious puberty. MAS is due to activating mutations of GNAS, the gene encoding Gs alpha and mutations analysis of this gene could increase the definitive diagnosis of MAS and atypical and partial. Objectives: To identify the p.R201H and p. R201C GNAS activating mutations in multiple tissues derived from patients with MAS using real time PCR genotyping. Material and methods: Genomic DNA was isolated from blood from 31 patients (28 females) with typical and atypical forms of MAS. Skin, adrenal gland or bone tissue samples were also available from six different patients. Genotyping based on PCR real time assay using TaqMan probes was performed for identification of p.R201H and p. R201C GNAS mutations. Cloning and sequencing were used as assenting techniques. Results: Using real time PCR genotyping, no mutations in GNAS were identified in blood samples of MAS patients, only in bone sample of a patient with a previously identified p.R201H. Cloning and sequencing from blood of this same patient revealed that 5/150 clones harboring the p. R201H. Conclusion: The real time PCR genotyping proved to be efficient for the molecular diagnosis of MAS in affected patient's tissues. Advantages of this technique are rapidity, accuracy, it is generally easy to perform and could be used routinely. Nevertheless, optimization of GNAS detection mutation is still necessary to considerer this technique to earlier diagnosis of non-classical forms of MAS using peripheral blood.","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"264 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2014-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73289431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Prospective Screening of Gene Copy Number Variation in Brazilian Admixed Population Sample 巴西混合群体样本基因拷贝数变异的前瞻性筛选

Bone Marrow Research Pub Date : 2014-01-31 DOI: 10.4172/2161-1041.1000125

Dianny Elizabeth Jimenez, T. C. Lins, Priscilla Orosco Taveira, R. Pereira

{"title":"A Prospective Screening of Gene Copy Number Variation in Brazilian Admixed Population Sample","authors":"Dianny Elizabeth Jimenez, T. C. Lins, Priscilla Orosco Taveira, R. Pereira","doi":"10.4172/2161-1041.1000125","DOIUrl":"https://doi.org/10.4172/2161-1041.1000125","url":null,"abstract":"Copy number variants (CNVs) represent an important source of variation in the human genome. Some CNVS embedded genes are differently distributed among the human population groups. Therefore, it is important to understand the distribution of CNV within and between populations, especially in those with admixed ancestry, such as the Brazilians. The aim of the study was to investigate the variability of a set of CNV-embedded genes in a sample of the Brazilian population. The CNV-embedded genes were chosen based on data showing that they have differential copy variation distribution between African and Europeans. Four genes (POLR2J4, PCDHB13, NPEPPS and AMY1) were investigated by qPCR in a sample of 96 Brazilians, previously classified by genetic ancestry. The gene AMY1 showed a variable copy numbers in the range of 1 to 8 copies whereas NPEPPS ranged from 1 to 5 copies. A low variability was identified for the POLR2J4 and PCDHB13 genes, showing 2 copies in frequency of 0.875 and 0.917, respectively. Genetic ancestry was not correlated to the number of copies of the AMY1and NPEPPS genes. The results provided an overview of the corresponding frequency of gene copy number variation in a sample of the Brazilian population, serving as reference for further genetic population studies, which may correlate these polymorphisms with other phenotypic features.","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"18 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2014-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81945505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Myelonecrosis: A Clinicopathological Study from a Tertiary Care Center in South India over a Twelve-Year Period. 脊髓坏死:一项来自南印度三级保健中心的临床病理研究，为期12年。

Bone Marrow Research Pub Date : 2014-01-01 Epub Date: 2014-01-23 DOI: 10.1155/2014/890510

Jinkala Sree Rekha, Rakhee Kar, Debdatta Basu

{"title":"Myelonecrosis: A Clinicopathological Study from a Tertiary Care Center in South India over a Twelve-Year Period.","authors":"Jinkala Sree Rekha, Rakhee Kar, Debdatta Basu","doi":"10.1155/2014/890510","DOIUrl":"https://doi.org/10.1155/2014/890510","url":null,"abstract":"Aims. To study the etiology, diagnostic features, and clinical significance of myelonecrosis. Methods. A retrospective review of all trephine biopsies done over 12 years (January 2000 to December 2012) in Department of pathology was done and all trephine biopsies showing MN were identified and studied. Results. Twenty-five cases accounting for 0.4% were identified. Fever and generalized weakness were the common presenting symptoms. Anemia was seen in all cases followed by thrombocytopaenia. Malignancy was the underlying cause in 64% of cases; hematolymphoid malignancy was seen in two-thirds and solid malignancies in one-third of the cases. Tuberculosis accounted for 16% of the cases and the etiology was unknown in 12%. Conclusions. The causes of MN are varied and hematological malignancy and solid malignancies are the most common causes. Presence of myelonecrosis is associated with a poor prognosis. Myelonecrosis may obscure the underlying disorder and hence a thorough search in the bone marrow biopsy itself with the help of immunohistochemistry may prove worthwhile in identifying the underlying disease. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2014 ","pages":"890510"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/890510","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32168419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient. 骨髓细胞亚群沿SDF-1α和ATP梯度的选择性迁移。

Bone Marrow Research Pub Date : 2014-01-01 Epub Date: 2014-12-31 DOI: 10.1155/2014/182645

Michael Laupheimer, Anna Skorska, Jana Große, Gudrun Tiedemann, Gustav Steinhoff, Robert David, Cornelia A Lux

{"title":"Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient.","authors":"Michael Laupheimer, Anna Skorska, Jana Große, Gudrun Tiedemann, Gustav Steinhoff, Robert David, Cornelia A Lux","doi":"10.1155/2014/182645","DOIUrl":"https://doi.org/10.1155/2014/182645","url":null,"abstract":"Both stem cell chemokine stromal cell-derived factor-1α (SDF-1α) and extracellular nucleotides such as adenosine triphosphate (ATP) are increased in ischemic myocardium. Since ATP has been reported to influence cell migration, we analysed the migratory response of bone marrow cells towards a combination of SDF-1 and ATP. Total nucleated cells (BM-TNCs) were isolated from bone marrow of cardiac surgery patients. Migration assays were performed in vitro. Subsequently, migrated cells were subjected to multicolor flow cytometric analysis of CD133, CD34, CD117, CD184, CD309, and CD14 expression. BM-TNCs migrated significantly towards a combination of SDF-1 and ATP. The proportions of CD34+ cells as well as subpopulations coexpressing multiple stem cell markers were selectively enhanced after migration towards SDF-1 or SDF-1 + ATP. After spontaneous migration, significantly fewer stem cells and CD184+ cells were detected. Direct incubation with SDF-1 led to a reduction of CD184+ but not stem cell marker-positive cells, while incubation with ATP significantly increased CD14+ percentage. In summary, we found that while a combination of SDF-1 and ATP elicited strong migration of BM-TNCs in vitro, only SDF-1 was responsible for selective attraction of hematopoietic stem cells. Meanwhile, spontaneous migration of stem cells was lower compared to BM-TNCs or monocytes. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2014 ","pages":"182645"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/182645","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32993820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Molecular regulation of bone marrow metastasis in prostate and breast cancer. 前列腺癌和乳腺癌骨髓转移的分子调控。

Bone Marrow Research Pub Date : 2014-01-01 Epub Date: 2014-07-23 DOI: 10.1155/2014/405920

Fakher Rahim, Saeideh Hajizamani, Esmaeil Mortaz, Ahmad Ahmadzadeh, Mohammad Shahjahani, Saeid Shahrabi, Najmaldin Saki

{"title":"Molecular regulation of bone marrow metastasis in prostate and breast cancer.","authors":"Fakher Rahim, Saeideh Hajizamani, Esmaeil Mortaz, Ahmad Ahmadzadeh, Mohammad Shahjahani, Saeid Shahrabi, Najmaldin Saki","doi":"10.1155/2014/405920","DOIUrl":"https://doi.org/10.1155/2014/405920","url":null,"abstract":"Metastasis is a multistep process, which refers to the ability to leave a primary tumor through circulation toward the distant tissue and form a secondary tumor. Bone is a common site of metastasis, in which osteolytic and osteoblastic metastasis are observed. Signaling pathways, chemokines, growth factors, adhesion molecules, and cellular interactions as well as miRNAs have been known to play an important role in the development of bone metastasis. These factors provide an appropriate environment (soil) for growth and survival of metastatic tumor cells (seed) in bone marrow microenvironment. Recognition of these factors and determination of their individual roles in the development of metastasis and disruption of cellular interactions can provide important therapeutic targets for treatment of these patients, which can also be used as prognostic and diagnostic biomarkers. Thus, in this paper, we have attempted to highlight the molecular regulation of bone marrow metastasis in prostate and breast cancers. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2014 ","pages":"405920"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/405920","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32605678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Hepatitis C among Egyptian Patients Referred for Bone Marrow Examination: Seroprevalence and Analysis of Hematological Findings. 接受骨髓检查的埃及丙型肝炎患者:血清阳性率和血液学结果分析。

Bone Marrow Research Pub Date : 2014-01-01 Epub Date: 2014-04-10 DOI: 10.1155/2014/549716

Somaia Mohammed Mousa

{"title":"Hepatitis C among Egyptian Patients Referred for Bone Marrow Examination: Seroprevalence and Analysis of Hematological Findings.","authors":"Somaia Mohammed Mousa","doi":"10.1155/2014/549716","DOIUrl":"https://doi.org/10.1155/2014/549716","url":null,"abstract":"Hepatitis C is a significant public health problem in Egypt where the highest prevalence (14.7%) of hepatitis C virus (HCV) exists. HCV prevalence is even higher among clinical populations and groups at risk of exposure to infection. Chronic HCV infection is associated with several hematological complications that may necessitate bone marrow (BM) examination. The aim of this study is to estimate HCV prevalence among patients referred for BM examination and to explore hematological and BM findings among HCV positive patients. One hundred adult patients referred for BM examination were included in the study and screened for HCV antibodies. Patients' clinical, hematological, and BM findings were recorded. The seroprevalence of HCV among patients referred for BM examination was 42%. The most common indication for BM examination among HCV positive patients was peripheral cytopenias (88.1%). The most common cytopenia detected was thrombocytopenia (85.7%). The most common diagnosis among HCV positive patients was hypersplenism (52.4%) followed by B-lymphoproliferative disorders (19%) and then immune thrombocytopenic purpura (11.9%). In conclusion, HCV prevalence among patients referred for BM examination is higher than that estimated in the general population. Patients with unexplained peripheral cytopenias should be tested for HCV. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2014 ","pages":"549716"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/549716","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32332622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Combination of Complement-Dependent Cytotoxicity and Relative Fluorescent Quantification of HLA Length Polymorphisms Facilitates the Detection of a Loss of Heterozygosity. 补体依赖性细胞毒性和HLA长度多态性的相对荧光定量相结合有助于检测杂合性缺失。

Bone Marrow Research Pub Date : 2014-01-01 Epub Date: 2014-04-03 DOI: 10.1155/2014/541345

Klaus Witter, Roland Reibke, Marion Subklewe, Robert Zahn, Teresa Kauke, Karsten Spiekermann, Michael Spannagl, Johanna Tischer, Wolfgang Hiddemann, Andrea Dick

{"title":"Combination of Complement-Dependent Cytotoxicity and Relative Fluorescent Quantification of HLA Length Polymorphisms Facilitates the Detection of a Loss of Heterozygosity.","authors":"Klaus Witter, Roland Reibke, Marion Subklewe, Robert Zahn, Teresa Kauke, Karsten Spiekermann, Michael Spannagl, Johanna Tischer, Wolfgang Hiddemann, Andrea Dick","doi":"10.1155/2014/541345","DOIUrl":"https://doi.org/10.1155/2014/541345","url":null,"abstract":"Loss of heterozygosity (LOH) is a common event in malignant cells. In this work we introduce a new approach to identify patients with loss of heterozygosity in the HLA region either at first diagnosis or after HLA mismatched allogeneic HSCT. Diagnosis of LOH requires a high purity of recipient target cells. FACS is time consuming and also frequently prevented by rather nonspecific or unknown immune phenotype. The approach for recipient cell enrichment is based on HLA targeted complement-dependent cytotoxicity (CDC). Relative fluorescent quantification (RFQ) analysis of HLA intron length polymorphisms then allows analysis of HLA heterozygosity. The approach is exemplified in recent clinical cases illustrating the detection of an acquired allele loss. As illustrated in one case with DPB1, distinct HLA loci in donor and patient were sufficient for both proof of donor cell removal and evaluation of allele loss in the patient's leukemic cells. Results were confirmed using HLA-B RFQ analysis and leukemia-associated aberrant immunophenotype (LAIP) based cell sort. Both results confirmed suspected loss of HLA heterozygosity. Our approach complements or substitutes for FACS-based cell enrichment; hence it may be further developed as novel routine diagnostic tool. This allows rapid recipient cell purification and testing for loss of HLA heterozygosity before and after allogeneic HSCT in easily accessible peripheral blood samples. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2014 ","pages":"541345"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/541345","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32368203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Angiogenesis and proliferation index in patients with acute leukemia: a prospective study. 急性白血病患者血管生成和增殖指数的前瞻性研究。

Bone Marrow Research Pub Date : 2014-01-01 Epub Date: 2014-03-31 DOI: 10.1155/2014/634874

Prabhavati Jothilingam, Debdatta Basu, Tarun K Dutta

{"title":"Angiogenesis and proliferation index in patients with acute leukemia: a prospective study.","authors":"Prabhavati Jothilingam, Debdatta Basu, Tarun K Dutta","doi":"10.1155/2014/634874","DOIUrl":"https://doi.org/10.1155/2014/634874","url":null,"abstract":"Angiogenesis and proliferation as measured by microvessel density (MVD) and proliferation index (PI) are essential correlates of malignancy. The aim of our study was to evaluate difference between these values in AML and ALL and also to study the modulation in these parameters following achievement of remission in acute lymphoblastic leukemia. Differences between adult and adolescent cases of acute leukemia in relation to these values were also studied. We also tried to assess the relationship between angiogenesis and proliferation. Fifty-five patients with acute leukemia were included in the study. Trephine biopsies were immunostained with CD34 and factor VIIIrAg to demonstrate angiogenesis measured as MVD. Immunostaining with PCNA and Ki-67 was done to study proliferation. We found a significant increase in MVD and PI in cases when compared with controls (P < 0.0001). In addition cases with ALL had a significantly higher MVD compared to those with AML (P < 0.01). The patients with ALL who went into remission showed a significant reduction in MVD; PI remained high. The cases which did not achieve remission showed no significant reduction in either MVD or PI. All adolescent cases of ALL were similar to adults with respect to MVD and PI. ","PeriodicalId":9220,"journal":{"name":"Bone Marrow Research","volume":"2014 ","pages":"634874"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/634874","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32319037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11