British Journal of Applied Science and Technology最新文献_第5页

Automated Railway Crossing System Using ZigBee/IEEE 802.15.4 Standard 采用ZigBee/IEEE 802.15.4标准的自动铁路道口系统

British Journal of Applied Science and Technology Pub Date : 2017-12-15 DOI: 10.14416/j.ijast.2017.12.005

Muhammad Mansattha, W. Benjapolakul

引用次数: 3

Characterization and kinetic parameters of endo-1,4-β-d-glucanase of ultraviolet mutated Aspergillus niger and Penicillium citrinum 紫外诱变黑曲霉和柠檬青霉内毒素-1,4-β-d-葡聚糖酶的表征及动力学参数

British Journal of Applied Science and Technology Pub Date : 2017-01-01 DOI: 10.4314/JAST.V22I1-2

M. Boadu, S. Inkoom, C. Schandorf, G. Emi-Reynolds, E. Akrobortu

{"title":"Characterization and kinetic parameters of endo-1,4-β-d-glucanase of ultraviolet mutated Aspergillus niger and Penicillium citrinum","authors":"M. Boadu, S. Inkoom, C. Schandorf, G. Emi-Reynolds, E. Akrobortu","doi":"10.4314/JAST.V22I1-2","DOIUrl":"https://doi.org/10.4314/JAST.V22I1-2","url":null,"abstract":"Mutagenic agents had been used to enhance production of enzymes in microorganisms, but the effects of the agents on the kinetic parameters and catalytic efficiency of the produced enzymes had not been critically evaluated. A study was conducted to assess the effects of ultraviolet (UV) mutagenesis on the kinetic parameters and catalytic efficiency of endo-1,4-β-D-glucanase enzyme obtained from wild and mutated strains of Aspergillus niger and Penicillium citrinum obtained from decaying wood wastes modified by ultraviolet radiation. The endo-1, 4-β-Dglucanase from both wild and mutant strains of the fungi were purified by centrifugation, ammonium sulfa-te precipitation and anion exchange chromatography using Sephadex A25-120 and Whatman DE-52 resins. The kinetic parameters determined from Lineweaver-Burk plot, using the velocity or reaction rates at varying concentrations of carboxymethyl cellulose substrate ranging from 0 - 20 mg/mL were validated by Hanes-Woolf plots for convergence, as the Michaelis-Menten kinetic equation is non-linear. The Michaelis-Menten constant (Km) and maximum velocity (Vmax) for the wild A. niger were 15.31 g/L and 4.67 g/L respectively, while values for corresponding mutated strain were 7.9 g/L and 3.39 g/L respectively. The Km and Vmax for wild P. citrinum were 7.94g/L and 6.81g/L respectively, while the values for the mutated strain were 6.60 g/L and 6.84 g/L respectively. Catalytic efficiency of endoglucanase of wild and mutated strains of A. niger were 0.305 and 0.42 M-1s-1 respectively, while the values for endoglucanase of wild and mutated strains of P. citrinum were 0.858 and 1.036 M-1s-1 respectively. Mutagenesis using ultraviolet radiation therefore enhanced the catalytic efficiency of endoglucanase enzyme.Keywords: Endo-1,4-β-D-glucanase, Aspergillus niger, Penicillium citrinum, UV Mutagenesis, Michaelis-Menten Kinetic Equation, Lineweaver-Burk Plot, Hanes Woolf Plot, Catalytic Efficiency","PeriodicalId":9207,"journal":{"name":"British Journal of Applied Science and Technology","volume":"15 12 1","pages":"28-36"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86921650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Theoretical analysis of hydriding reactions of ZrCo and LaNi 5 intermetallic alloys using non-isothermal shrinking core model 用非等温收缩核模型分析ZrCo和lani5金属间合金的氢化反应

British Journal of Applied Science and Technology Pub Date : 2016-01-01 DOI: 10.4314/JAST.V21I1-2

R. Bhattacharyya

引用次数: 0

Trends Analysis and Future of Sustainable Palm Oil in Thailand 泰国可持续棕榈油的趋势分析和未来

British Journal of Applied Science and Technology Pub Date : 2015-02-10 DOI: 10.14416/J.IJAST.2015.01.001

U. Wangrakdiskul, N. Yodpijit

引用次数: 9

Statistical Study Of Variation Of Diffuse Solar Radiation Over Nigeria 尼日利亚上空太阳漫射辐射变化的统计研究

British Journal of Applied Science and Technology Pub Date : 2015-01-01 DOI: 10.4314/JAST.V20I1-2

E. Falayi, A. Rabiu

引用次数: 0

Identification of Volatile Compounds in Jellyfish Protein Hydrolysate 水母蛋白水解物挥发性化合物的鉴定

British Journal of Applied Science and Technology Pub Date : 2014-12-19 DOI: 10.14416/J.IJAST.2014.10.003

Isaree Kromfang, Utorn Chikhunthod, Pirin Karpilanondh, B. Thumthanaruk

引用次数: 1

Resistance Measurement of Conductive Thermoplastic Bipolar Plates for Polymer Electrolyte Membrane Fuel Cells 聚合物电解质膜燃料电池用导电热塑性双极板的电阻测量

British Journal of Applied Science and Technology Pub Date : 2014-09-29 DOI: 10.14416/J.IJAST.2014.08.001

R. Yeetsorn, M. Fowler

引用次数: 6

Principal component analysis of groundwater quality data underlying Geochemical processes of Dahomeyan formation and Togo series in the Ho Municipality (Ghana) 加纳Ho市达梅扬组和多哥系地球化学过程地下水水质数据的主成分分析

British Journal of Applied Science and Technology Pub Date : 2014-09-26 DOI: 10.4314/JAST.V19I1-2

I. Hodgson, S. Obiri, S. Cobbina, G. Quarcoo, AA Duah

{"title":"Principal component analysis of groundwater quality data underlying Geochemical processes of Dahomeyan formation and Togo series in the Ho Municipality (Ghana)","authors":"I. Hodgson, S. Obiri, S. Cobbina, G. Quarcoo, AA Duah","doi":"10.4314/JAST.V19I1-2","DOIUrl":"https://doi.org/10.4314/JAST.V19I1-2","url":null,"abstract":"The physico-chemical quality data of groundwater extracted from 36 boreholes in Ho Municipality indicated that 11 sources recorded pH values outside WHO guideline range, while 5 recorded turbidity levels above WHO guideline limits. Sodium levels ranged from 1.80 to 544 mg/L with a mean of 111 ± 132 mg/l; chloride level ranged from 2.98 to 898 mg/L with a mean of 124 ± 183 mg/L, and cation and anion dominance was in the ord-er Na+ > Mg 2+ > Ca 2+ > K + and HCO 3 - > Cl - > SO 4 2- respectively. The quality data was subjected to Principal Component Analysis, and two components (PC1 and PC2) were extracted. The PC1 represented nearly 88.17 % of the variance and had high absolute loadings for electrical conductivity, calcium, alkalinity and chloride, indi-cating interactions of minerals from the Dahomeyan formation and Togo series with the groundwater in the area. The PC2 accounted for 6.62 % of total variance, showing high loadings for colour, turbidity, HCO 3 and total iron. The major geochemical processes taking place might be the presence and dissolution of carbonates and dolomites, evaporated minerals and chemical reactions involving reverse ion-exchange in the aquifer","PeriodicalId":9207,"journal":{"name":"British Journal of Applied Science and Technology","volume":"1 1","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"2014-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79916211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Performance and Exhaust Emission Studies of a Large LNG-Diesel Engine Operating with Different Gas Injector’s Characteristics 不同喷油器特性下大型lng -柴油发动机性能及排放研究

British Journal of Applied Science and Technology Pub Date : 2014-05-16 DOI: 10.14416/J.IJAST.2014.04.002

Chedthawut Poompipatpong

{"title":"Performance and Exhaust Emission Studies of a Large LNG-Diesel Engine Operating with Different Gas Injector’s Characteristics","authors":"Chedthawut Poompipatpong","doi":"10.14416/J.IJAST.2014.04.002","DOIUrl":"https://doi.org/10.14416/J.IJAST.2014.04.002","url":null,"abstract":"This research article reports the performances and emissions of a 12-litre LNG-diesel dual-fuelled engine when equipped with two different gas injectors. Natural gas is used as main fuel with a pilot amount of diesel for the ignition source. The main objective of this study is to replace the imported injector by the local product, while maintaining the performance compatibility. This means an economical benefit is obtained. Both gas injectors’ characteristics are investigated and found that characteristic of local product is relatively different from the imported one. The results show that injectors’ characteristic analysis must be separated into two ranges, including lower and higher engine speed ranges. Thus, the local injector must be then electronically adjusted in order to attain the engine performance and emission compatibilities. Consequently, it can operate satisfyingly, while no engine knocking is observed. The engine performance results between two injectors show minor differences, which are averagely less than 0.43%. The exhaust emissions also show small differences. The graphical presentations, the discussions and conclusions are also presented in this paper.","PeriodicalId":9207,"journal":{"name":"British Journal of Applied Science and Technology","volume":"7 1","pages":"59-66"},"PeriodicalIF":0.0,"publicationDate":"2014-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89032076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Multi-materials Composites Provide Lightweight Engineering 多材料复合材料提供轻量化工程

British Journal of Applied Science and Technology Pub Date : 2014-04-28 DOI: 10.14416/J.IJAST.2014.04.001

A. Frick, M. Borm

引用次数: 1