Biochimie open最新文献

AhR signaling pathways and regulatory functions AhR信号通路及其调控功能

Biochimie open Pub Date : 2018-12-01 DOI: 10.1016/j.biopen.2018.05.001

Lucie Larigot , Ludmila Juricek , Julien Dairou , Xavier Coumoul

引用次数: 320

Retroviral nucleocapsid proteins and DNA strand transfers 逆转录病毒核衣壳蛋白和DNA链转移

Biochimie open Pub Date : 2018-12-01 DOI: 10.1016/j.biopen.2018.07.001

Brigitte René, Olivier Mauffret, Philippe Fossé

引用次数: 8

Vanadate inhibits transcription of the rat insulin receptor gene via a proximal sequence of the 5′flanking region 钒酸盐通过5 '侧区近端序列抑制大鼠胰岛素受体基因的转录

Biochimie open Pub Date : 2018-12-01 DOI: 10.1016/j.biopen.2018.09.001

Sylvie Bortoli , Martine Collinet , Bernard Desbuquois

{"title":"Vanadate inhibits transcription of the rat insulin receptor gene via a proximal sequence of the 5′flanking region","authors":"Sylvie Bortoli , Martine Collinet , Bernard Desbuquois","doi":"10.1016/j.biopen.2018.09.001","DOIUrl":"10.1016/j.biopen.2018.09.001","url":null,"abstract":"<div><p>Vanadate, a protein tyrosine phosphatase inhibitor which elicits insulin-like effects, has previously been shown to inhibit expression of the insulin receptor gene at the transcriptional level in rat hepatoma cells. In an attempt to identify the DNA sequence and transcription factors potentially involved in this effect, a fragment of the proximal 5′flanking region of the IR gene (−1143/−252 upstream the ATG codon) has been cloned and functionally characterized. RNase protection allowed the identification of several transcription start sites in the conserved region of the gene, among which two major sites at −455 and −396. Upon fusion to the luciferase gene and transient transfection into hepatoma cells, the −1143/−252 fragment showed promoter activity. This was unaffected by deletion of the −1143/−761 sequence, but markedly decreased (90%) by additional deletion of the −760/−465 sequence. Treatment of hepatoma cells with vanadate led to a dose-dependent decrease in promoter activity of the 1143/−252, −760/−252 and −464/−252 constructs (change relative to untreated cells, 40, 55 and 23% at 125 μM, and 70, 85 and 62% at 250 μM, respectively). These data suggest that although the entire DNA sequence upstream the transcription start sites is probably involved in vanadate-induced inhibition, the short sequence downstream of position −464 and is sufficient for inhibition. Potential targets of vanadate are the transcription factors FoxO1 and HMGA1, two downstream targets of the insulin signaling pathway which have been shown to mediate the inhibitory effect of insulin on IR gene expression.</p></div>","PeriodicalId":92004,"journal":{"name":"Biochimie open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biopen.2018.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36667047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Acknowledgement to Reviewers 审稿人致谢

Biochimie open Pub Date : 2018-12-01 DOI: 10.1016/j.biopen.2018.12.001

引用次数: 0

Isoprostanes as markers for muscle aging in older athletes 异前列腺素作为老年运动员肌肉老化的标志

Biochimie open Pub Date : 2018-06-01 DOI: 10.1016/j.biopen.2017.12.001

Claire Vinel , Ophélie Pereira , Aude Dupuy , Justine Bertrand-Michel , Dalila Laoudj-Chenivesse , Yves Rolland , Daniel Rivière , Philippe Valet , Cédric Dray , Fabien Pillard

{"title":"Isoprostanes as markers for muscle aging in older athletes","authors":"Claire Vinel , Ophélie Pereira , Aude Dupuy , Justine Bertrand-Michel , Dalila Laoudj-Chenivesse , Yves Rolland , Daniel Rivière , Philippe Valet , Cédric Dray , Fabien Pillard","doi":"10.1016/j.biopen.2017.12.001","DOIUrl":"10.1016/j.biopen.2017.12.001","url":null,"abstract":"<div><h3>Introduction</h3><p>Production of isoprostanes (IsoPs) is enhanced after acute, intense, and prolonged exercise, in untrained subjects. This effect is greater in older subjects. The present study aims to delineate the profile of acute-exercise-induced IsoPs levels in young and older endurance-trained subjects.</p></div><div><h3>Methods</h3><p>All included subjects were male, young (n = 6; 29 yrs ± 5.7) or older (n = 6; 63.7 yrs ± 2.3), and competitors. The kinetics of F<sub>2</sub>-IsoPs in blood-sera was assessed at rest, for the maximal aerobic exercise power (MAP) corresponding to the cardio-respiratory fitness index and after a 30-min recovery period.</p></div><div><h3>Results</h3><p>No significant time effect on F<sub>2</sub>-IsoPs kinetics was identified in young subjects. However, in older athletes, F<sub>2</sub>-IsoPs blood-concentrations at the MAP were higher than at rest, whereas these blood-concentrations did not differ between rest and after the 30-min recovery period.</p></div><div><h3>Conclusion</h3><p>Because plasma glutathione (GSH) promotes the formation of some F<sub>2</sub>-IsoPs, we suggest that the surprising decrease in F<sub>2</sub>-IsoPs levels in older subjects would be caused by decreased GSH under major ROS production in older subjects. We argue that the assessment F<sub>2</sub>-IsoPs in plasma as biomarkers of the aging process should be challenged by exercise to improve the assessment of the functional response against reactive oxygen species in older subjects.</p></div>","PeriodicalId":92004,"journal":{"name":"Biochimie open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biopen.2017.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36214851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Glycan binding profile of a fucolectin-related protein (FRP) encoded by the SP2159 gene of Streptococcus pneumoniae 肺炎链球菌SP2159基因编码的岩藻胶蛋白相关蛋白(FRP)的聚糖结合谱

Biochimie open Pub Date : 2018-06-01 DOI: 10.1016/j.biopen.2017.12.002

Albert M. Wu , Tanuja Singh , Yung Liang Chen , Kimberly M. Anderson , Su Chen Li , Yu Teh Li

引用次数: 2

Herbal genomics as tools for dissecting new metabolic pathways of unexplored medicinal plants and drug discovery 草药基因组学作为剖析未开发药用植物新代谢途径和药物发现的工具

Biochimie open Pub Date : 2018-06-01 DOI: 10.1016/j.biopen.2017.12.003

Prasanta Chakraborty

引用次数: 53

Lack of current observed in HEK293 cells expressing NALCN channels 在表达NALCN通道的HEK293细胞中观察到缺乏电流

Biochimie open Pub Date : 2018-06-01 DOI: 10.1016/j.biopen.2018.01.001

Jennifer M. Egan, Colleen A. Peterson, W. Mark Fry

{"title":"Lack of current observed in HEK293 cells expressing NALCN channels","authors":"Jennifer M. Egan, Colleen A. Peterson, W. Mark Fry","doi":"10.1016/j.biopen.2018.01.001","DOIUrl":"10.1016/j.biopen.2018.01.001","url":null,"abstract":"<div><p>The sodium leak channel NALCN is poorly understood, but is reported as a Na<sup>+</sup>-permeable, nonselective cation leak channel which regulates resting membrane potential and electrical excitability. Previous work has indicated that NALCN currents can be stimulated by activation of several G protein coupled receptors, including the M3 muscarinic receptor. We undertook a study using voltage clamp electrophysiology to investigate NALCN currents. We compared currents elicited from untransfected control HEK239 cells in response to M3R agonists muscarine or Oxotremorine M to currents elicited from cells transfected with M3R only or the M3R plus NALCN and cDNA encoding accessory proteins UNC-80 and Src. Currents with similar properties were observed in all three groups of cells in response to muscarine agonists, in similar proportions of cells tested, from all three groups of cells. Our findings do not support previous electrophysiological studies suggesting that heterologously expressed NALCN functions as a Na<sup>+</sup> leak channel in HEK293 cells. More research will be required to determine the molecular requirements for successful expression of the NALCN channel.</p></div>","PeriodicalId":92004,"journal":{"name":"Biochimie open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biopen.2018.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36214510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Chemical composition analysis and in vitro biological activities of ten essential oils in human skin cells 十种精油在人体皮肤细胞中的化学成分分析及体外生物活性

Biochimie open Pub Date : 2017-12-01 DOI: 10.1016/j.biopen.2017.04.001

Xuesheng Han, Cody Beaumont, Nicole Stevens

{"title":"Chemical composition analysis and in vitro biological activities of ten essential oils in human skin cells","authors":"Xuesheng Han, Cody Beaumont, Nicole Stevens","doi":"10.1016/j.biopen.2017.04.001","DOIUrl":"10.1016/j.biopen.2017.04.001","url":null,"abstract":"<div><p>Research on the biological effects of essential oils on human skin cells is scarce. In the current study, we primarily explored the biological activities of 10 essential oils (nine single and one blend) in a pre-inflamed human dermal fibroblast system that simulated chronic inflammation. We measured levels of proteins critical for inflammation, immune responses, and tissue-remodeling processes. The nine single oils were distilled from <em>Citrus bergamia</em> (bergamot), <em>Coriandrum sativum</em> (cilantro), <em>Pelargonium graveolens</em> (geranium), <em>Helichrysum italicum</em> (helichrysum), <em>Pogostemon cablin</em> (patchouli), <em>Citrus aurantium</em> (petitgrain), <em>Santalum album</em> (sandalwood), <em>Nardostachys jatamansi</em> (spikenard), and <em>Cananga odorata</em> (ylang ylang). The essential oil blend (commercial name Immortelle) is composed of oils from frankincense, Hawaiian sandalwood, lavender, myrrh, helichrysum, and rose. All the studied oils were significantly anti-proliferative against these cells. Furthermore, bergamot, cilantro, and spikenard essential oils primarily inhibited protein molecules related to inflammation, immune responses, and tissue-remodeling processes, suggesting they have anti-inflammatory and wound healing properties. Helichrysum and ylang ylang essential oils, as well as Immortelle primarily inhibited tissue remodeling-related proteins, suggesting a wound healing property. The data are consistent with the results of existing studies examining these oils in other models and suggest that the studied oils may be promising therapeutic candidates. Further research into their biological mechanisms of action is recommended. The differential effects of these essential oils suggest that they exert activities by different mechanisms or pathways, warranting further investigation. The chemical composition of these oils was analyzed using gas chromatography–mass spectrometry.</p></div>","PeriodicalId":92004,"journal":{"name":"Biochimie open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biopen.2017.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35837008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Acknowledgement to Reviewers 审稿人致谢

Biochimie open Pub Date : 2017-12-01 DOI: 10.1016/j.biopen.2017.11.001

引用次数: 0