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Fermentation Microbiology and Biotechnology E.M.T. El-Mansi and C.F.A. Bryce (Eds.) 发酵微生物学和生物技术E.M.T. El-Mansi和C.F.A. Bryce(编)
Bioseparation Pub Date : 2000-07-01 DOI: 10.1023/A:1008192103524
J. Kennedy, N. Turan
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引用次数: 1
Superporous agarose monoliths as mini-reactors in flow injection systems 超多孔琼脂糖单体在流动注射系统中的微型反应器
Bioseparation Pub Date : 2000-07-01 DOI: 10.1023/A:1008117827057
Madayi P. Nandakumar, E. Pålsson, P. Gustavsson, P. Larsson, B. Mattiasson
{"title":"Superporous agarose monoliths as mini-reactors in flow injection systems","authors":"Madayi P. Nandakumar, E. Pålsson, P. Gustavsson, P. Larsson, B. Mattiasson","doi":"10.1023/A:1008117827057","DOIUrl":"https://doi.org/10.1023/A:1008117827057","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"15 1","pages":"193-202"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75274638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
Biological Process Engineering A. T. Johnson 生物过程工程
Bioseparation Pub Date : 2000-07-01 DOI: 10.1023/A:1008105524174
J. Kennedy, M. Thorley
{"title":"Biological Process Engineering A. T. Johnson","authors":"J. Kennedy, M. Thorley","doi":"10.1023/A:1008105524174","DOIUrl":"https://doi.org/10.1023/A:1008105524174","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"90 1","pages":"256-257"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88540224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Chromatography and Capillary Electrophoresis in Food Analysis H. Sorensen, S. Sorensen, C.Bjergegaard and S. Michaelsen 色谱和毛细管电泳在食品分析中的应用
Bioseparation Pub Date : 2000-07-01 DOI: 10.1023/A:1008182232750
J. Kennedy, Lorraine A. Quinton
{"title":"Chromatography and Capillary Electrophoresis in Food Analysis H. Sorensen, S. Sorensen, C.Bjergegaard and S. Michaelsen","authors":"J. Kennedy, Lorraine A. Quinton","doi":"10.1023/A:1008182232750","DOIUrl":"https://doi.org/10.1023/A:1008182232750","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"3 1","pages":"255"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88284976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Freeze-Drying/ Lyophilization of Pharmaceutical and Biological Products, Louis Rey and Joan C. May 制药和生物制品的冷冻干燥/冻干，Louis Rey和Joan C. May
Bioseparation Pub Date : 2000-03-01 DOI: 10.1023/A:1008125212856
J. Kennedy, N. Turan
{"title":"Freeze-Drying/ Lyophilization of Pharmaceutical and Biological Products, Louis Rey and Joan C. May","authors":"J. Kennedy, N. Turan","doi":"10.1023/A:1008125212856","DOIUrl":"https://doi.org/10.1023/A:1008125212856","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"5 1","pages":"118"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81951837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Immobilized Biomolecules in Analysis: A Practical Approach, Tony Cass & Frances S. Ligler (Eds.) 固定化生物分子分析:一种实用的方法，托尼卡斯和弗朗西斯S.利格勒(编)
Bioseparation Pub Date : 2000-03-01 DOI: 10.1023/A:1008192009124
J. Kennedy, N. Turan
{"title":"Immobilized Biomolecules in Analysis: A Practical Approach, Tony Cass & Frances S. Ligler (Eds.)","authors":"J. Kennedy, N. Turan","doi":"10.1023/A:1008192009124","DOIUrl":"https://doi.org/10.1023/A:1008192009124","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"59 1","pages":"117-118"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89649584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
HPLC of Macromolecules, 2 nd Edition, R.W.A. Oliver (Editor) 大分子高效液相色谱，第2版，R.W.A. Oliver(编辑)
Bioseparation Pub Date : 2000-03-01 DOI: 10.1023/A:1008126231841
J. Kennedy, Lorraine A. Quinton
{"title":"HPLC of Macromolecules, 2 nd Edition, R.W.A. Oliver (Editor)","authors":"J. Kennedy, Lorraine A. Quinton","doi":"10.1023/A:1008126231841","DOIUrl":"https://doi.org/10.1023/A:1008126231841","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"23 1","pages":"117"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83737926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Two-dimensional polyacrylamide gel electrophoresis of the protease SP220K, a renal cell carcinoma marker. 肾细胞癌标志物蛋白酶SP220K的双向聚丙烯酰胺凝胶电泳。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008198521231
M Starita-Geribaldi, S Thaon-Scarzello, M Le Blanc, E Van Obberghen, C Poustis-Delpont
{"title":"Two-dimensional polyacrylamide gel electrophoresis of the protease SP220K, a renal cell carcinoma marker.","authors":"M Starita-Geribaldi,&nbsp;S Thaon-Scarzello,&nbsp;M Le Blanc,&nbsp;E Van Obberghen,&nbsp;C Poustis-Delpont","doi":"10.1023/a:1008198521231","DOIUrl":"https://doi.org/10.1023/a:1008198521231","url":null,"abstract":"<p><p>The present study analyses, by two-dimensional polyacrylamide gel electrophoresis, the protease SP220K isolated from renal cell carcinoma. The pure molecule is separated using either immobilized pH gradient or isoelectric focusing in conventional carrier ampholyte in the first dimension. Some interactions with the acrylamide matrix in isoelectric focusing are discussed. The results demonstrate that two-dimensional gel electrophoresis performed with enriched media such as basolateral membranes, allows the detection of the protease. In addition, the non detection of the molecule up to now by this methodology can be explained by the high tendency of oligomerization of SP220K. Effectively the high molecular weight form of the molecule of 220 kDa is favoured in two-dimensional gel electrophoresis over monomeric forms which are better detected in SDS PAGE. This was confirmed by immunostaining performed with an antiserum to SP220K produced by nitrocellulose-bound antigen.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 3","pages":"133-44"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008198521231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21929127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Peptide fusion tags with tryptophan and charged residues for control of protein partitioning in PEG-potassium phosphate aqueous two-phase systems. 与色氨酸和带电荷残基的肽融合标签在peg -磷酸钾两水相体系中用于控制蛋白质分配。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008182711385
K Berggren, F Tjerneld, A Veide
{"title":"Peptide fusion tags with tryptophan and charged residues for control of protein partitioning in PEG-potassium phosphate aqueous two-phase systems.","authors":"K Berggren,&nbsp;F Tjerneld,&nbsp;A Veide","doi":"10.1023/a:1008182711385","DOIUrl":"https://doi.org/10.1023/a:1008182711385","url":null,"abstract":"<p><p>A partition study with peptides and recombinant proteins in poly(ethylene glycol)4000-potassium phosphate aqueous two-phase systems has been performed. The aim was to study to what extent the insertion of charged residues could affect protein partition in addition to the already observed effects of tryptophan residues. The model proteins used are based on a staphylococcal protein A derivative, Z, and modified by the insertion of peptide tags close to the C-terminus. The tags differed with respect to their content of both Trp, negatively (Asp) and positively charged (Lys) amino acid residues. The same partitioning trends were observed for the peptides and fusion proteins. The effect of Trp residues was to direct the partitioning towards the PEG phase. The insertion of two negatively charged (Asp) residues into a Trp4-tag enhanced the partition towards the PEG phase even more. The introduction of positively charged (Lys) residues in addition to Trp residues, on the other hand, pulled the peptide or protein towards the potassium phosphate phase. The partitioning of peptides gave a good qualitative picture of the effect of the peptide on partitioning when fused to the protein. The efficiencies of the tags were calculated based on partitioning of tags and fusion proteins, and tag efficiencies generally varied between 60 and 85%.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 2","pages":"69-80"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008182711385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21734410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Bovine serum albumin-hemoglobin fractionation: significance of ultrafiltration system and feed solution characteristics. 牛血清白蛋白-血红蛋白分离:超滤系统和饲料溶液特性的意义。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008194300403
R Shukla, M Balakrishnan, G P Agarwal
{"title":"Bovine serum albumin-hemoglobin fractionation: significance of ultrafiltration system and feed solution characteristics.","authors":"R Shukla,&nbsp;M Balakrishnan,&nbsp;G P Agarwal","doi":"10.1023/a:1008194300403","DOIUrl":"https://doi.org/10.1023/a:1008194300403","url":null,"abstract":"<p><p>This work investigates the fractionation of similar molecular weight proteins bovine serum albumin (69 kD) and bovine hemoglobin (67 kD) by ultrafiltration. Three different membranes, viz. regenerated cellulose, poly(sulfone) and surface modified poly(acrylonitrile), each with a nominal molecular cutoff rating of 100 kD, were examined. The experiments were conducted in dead end, crossflow and vortex flow filtration modes and the separation was studied as a function of feed pH and ionic strength. Under similar system hydrodynamics, the surface modified poly(acrylonitrile) membrane displayed the highest resolution with minimum membrane fouling. The separation could be improved further by operating at low applied pressure (40 kPa) and high mass transfer (> 20 x 10(-6) m/s) in a vortex flow module. Under these conditions, the highest separation factor of 40 was obtained at the pI of hemoglobin.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 1","pages":"7-19"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008194300403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21684692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
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