Biology of Blood and Marrow Transplantation最新文献

{"title":"Anaesthetic and Surgical Considerations in Post COVID-19 Patients Requiring Head and Neck Surgery.","authors":"Kritant Bhushan, Priya Jeyaraj, Rajnish Sahu, Mansi Luthra Sharma","doi":"10.1007/s12070-023-04040-5","DOIUrl":"10.1007/s12070-023-04040-5","url":null,"abstract":"<p><p>As the cases of COVID-19 have declined, the number of patients who have recovered from the dreaded disease is reporting for elective or emergency surgeries. Surgical planning in patients who have recovered from COVD-19 requires special considerations because of the morbidity and mortality associated with the infection and its devastating after-effects. There is a distinct paucity of literature on guidelines and protocols to follow in the perioperative management of these patients. With the help of experience gained over the past 2 years of the 'COVID-19 era', we have been able to establish important recommendations, guidelines and useful protocols during perioperative management of COVID-recovered patients. These protocols include important anesthetic and surgical considerations, which are both practical as well as implementable and are also in cognizance with government-laid down advisories. Although SARS-CoV-2 infection primarily affects the pulmonary and cardiac systems, it has the potential for serious and severely affect multiple organs and various other body systems in erratic and unpredictable manner. All of these factors can have significant implications that make the perioperative management of post-COVID-19 patients, difficult and challenging. Considering the far-reaching and long-lasting effects of this infection on the human body, the protocols and recommendations presented in this article can serve as a valuable guide for clinicians to effectively manage the surgical patient and help reduce perioperative complications attributable to COVID-19 infection.</p>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"18 1","pages":"3602-3609"},"PeriodicalIF":4.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75714357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-bound D-mannose isomerase of acetic acid bacteria: finding, characterization, and application.","authors":"Osao Adachi, Naoya Kataoka, Kazunobu Matsushita, Yoshihiko Akakabe, Toshihiro Harada, Toshiharu Yakushi","doi":"10.1093/bbb/zbac049","DOIUrl":"10.1093/bbb/zbac049","url":null,"abstract":"<p><p>D-Mannose isomerase (EC 5.3.1.7) catalyzing reversible conversion between D-mannose and D-fructose was found in acetic acid bacteria. Cell fractionation confirmed the enzyme to be a typical membrane-bound enzyme, while all sugar isomerases so far reported are cytoplasmic. The optimal enzyme activity was found at pH 5.5, which was clear contrast to the cytoplasmic enzymes having alkaline optimal pH. The enzyme was heat stable and the optimal reaction temperature was observed at around 40 to 60˚C. Purified enzyme after solubilization from membrane fraction showed the total molecular mass of 196 kDa composing of identical four subunits of 48 kDa. Washed cells or immobilized cells were well functional at nearly 80% of conversion ratio from D-mannose to D-fructose and reversely 20-25% of D-fructose to D-mannose. Catalytic properties of the enzyme were discussed with respect to the biotechnological applications to high fructose syrup production from konjac taro.</p>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"21 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75822028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human-induced pluripotent stem cells-derived retinal pigmented epithelium, a new horizon for cells-based therapies for age-related macular degeneration.","authors":"Samaneh Dehghan, Reza Mirshahi, Alireza Shoae-Hassani, Masood Naseripour","doi":"10.1186/s13287-022-02894-0","DOIUrl":"10.1186/s13287-022-02894-0","url":null,"abstract":"<p><p>Retinal pigment epithelium (RPE) degeneration is the hallmark of age-related macular degeneration (AMD). AMD, as one of the most common causes of irreversible visual impairment worldwide, remains in need of an appropriate approach to restore retinal function. Wet AMD, which is characterized by neovascular formation, can be stabilized by currently available therapies, including laser photocoagulation, photodynamic therapy, and intraocular injections of anti-VEFG (anti-vascular endothelial growth factor) therapy or a combination of these modalities. Unlike wet AMD, there is no effective therapy for progressive dry (non-neovascular) AMD. However, stem cell-based therapies, a part of regenerative medicine, have shown promising results for retinal degenerative diseases such as AMD. The goal of RPE cell therapy is to return the normal structure and function of the retina by re-establishing its interaction with photoreceptors, which is essential to vision. Considering the limited source of naturally occurring RPE cells, recent progress in stem cell research has allowed the generation of RPE cells from human pluripotent cells, both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSC). Since iPSCs face neither ethical arguments nor significant immunological considerations when compared to ESCs, they open a new horizon for cell therapy of AMD. The current study aims to discuss AMD, review the protocols for making human iPSCs-derived RPEs, and summarize recent developments in the field of iPSC-derived RPEs cell therapy.</p>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"11 1","pages":"217"},"PeriodicalIF":2.1,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76159986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Goal-Oriented Monitoring of Cyclosporine Is Effective for Graft-versus-Host Disease Prevention after Hematopoietic Stem Cell Transplantation in Sickle Cell Disease and Thalassemia Major","authors":"Alexandra Gauthier ,&nbsp;Nathalie Bleyzac ,&nbsp;Nathalie Garnier ,&nbsp;Kamila Kebaili ,&nbsp;Philippe Joly ,&nbsp;Marie-Pierre Goutagny ,&nbsp;Isabelle Mollet ,&nbsp;Sylvain Goutelle ,&nbsp;Cécile Renard ,&nbsp;Yves Bertrand","doi":"10.1016/j.bbmt.2020.01.016","DOIUrl":"10.1016/j.bbmt.2020.01.016","url":null,"abstract":"<div><p>Graft-versus-host disease (GVHD) is an important challenge and a major cause of morbidity and mortality in children after hematopoietic stem cell transplant (HSCT). Herein we report our institution's experience of goal-oriented Bayesian monitoring for cyclosporine (CsA) used alone as GVHD prophylaxis during the post-transplant period in pediatric patients with thalassemia major (TM) or sickle cell anemia (SCA) undergoing HLA-matched HSCT. We also studied evolution of chimerism. Twenty-six consecutive patients (SCA, 14; TM, 12) underwent matched sibling donor (MSD) HSCT from 2004 to 2014. All patients received a myeloablative conditioning regimen. GVHD prophylaxis consisted of 20 mg/kg antithymocyte globulin in the conditioning regimens and then CsA alone in the post-transplant period. Target CsA trough blood concentration (TBC) was 150 ± 20 ng/mL. At last follow-up, all patients were alive and free of disease, even in cases of mixed chimerism. Engraftment occurred in all patients. No patient developed grades II to IV acute GVHD, 4 patients developed acute grade I skin GVHD, and only 1 presented with chronic pulmonary GVHD. A better control of GVHD and immunosuppression by a strict monitoring of CsA TBC as described herein is promising and could play a crucial role. Further investigations are required, but this study opens new perspectives to improve survival and safety of HSCT from alternative donors in TM and SCA to levels compatible with that obtained with MSDs.</p></div>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"26 12","pages":"Pages 2285-2291"},"PeriodicalIF":4.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbmt.2020.01.016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37601574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-Hematopoietic Stem Cell Transplantation Lung Computed Tomography as an Alternative to the Pulmonary Function Test during the COVID-19 Pandemic","authors":"Masaharu Tamaki ,&nbsp;Hideki Nakasone ,&nbsp;Tadao Aikawa ,&nbsp;Yuhei Nakamura ,&nbsp;Masakatsu Kawamura ,&nbsp;Shunto Kawamura ,&nbsp;Junko Takeshita ,&nbsp;Nozomu Yoshino ,&nbsp;Yukiko Misaki ,&nbsp;Kazuki Yoshimura ,&nbsp;Shinpei Matsumi ,&nbsp;Ayumi Gomyo ,&nbsp;Aki Tanihara ,&nbsp;Machiko Kusuda ,&nbsp;Yu Akahoshi ,&nbsp;Shun-ichi Kimura ,&nbsp;Shinichi Kako ,&nbsp;Noriko Oyama-Manabe ,&nbsp;Yoshinobu Kanda","doi":"10.1016/j.bbmt.2020.08.025","DOIUrl":"10.1016/j.bbmt.2020.08.025","url":null,"abstract":"<div><p>The pulmonary function test (PFT) is an important test for risk stratification before allogeneic transplantation (allo-HCT). However, it might be preferable to avoid PFT as much as possible in the recent era of coronavirus disease 2019 (COVID-19), because PFT requires forced expirations and might produce aerosols, increasing the risk of COVID-19 transmission. Therefore, we tried to predict normal PFT results before allo-HCT based on computed tomography (CT) findings. This study included 390 allo-HCT recipients at our center for whom lung CT images and PFT results before allo-HCT were available. Abnormal CT findings were less likely to be observed in the normal PFT group (47.0% versus 67.4%, <em>P</em> = .015), with a high negative predictive value of 92.9%. In a multivariate analysis, normal CT was significantly associated with normal PFT (odds ratio, 2.47; 95% confidence interval, 1.22 to 4.97; <em>P</em> = .012). A model for predicting normal PFT was constructed based on the results of a multivariate analysis, and the area under the curve of the receiver operating characteristic analysis was 0.656, which gave a sensitivity of 45.5% and a specificity of 86.0%. The relatively high specificity of the model suggested that PFT can be omitted in patients with normal CT findings before allo-HCT.</p></div>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"26 12","pages":"Pages 2318-2322"},"PeriodicalIF":4.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbmt.2020.08.025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38323566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Officers and Directors of ASTCT","authors":"","doi":"10.1016/S1083-8791(20)30705-9","DOIUrl":"https://doi.org/10.1016/S1083-8791(20)30705-9","url":null,"abstract":"","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"26 12","pages":"Page A3"},"PeriodicalIF":4.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1083-8791(20)30705-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136816042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Neuroimaging Correlates of Post-Transplant Delirium","authors":"Patrick Smith ,&nbsp;Jillian C. Thompson ,&nbsp;Elena Perea ,&nbsp;Brian Wasserman ,&nbsp;Lauren Bohannon ,&nbsp;Alessandro Racioppi ,&nbsp;Taewoong Choi ,&nbsp;Cristina Gasparetto ,&nbsp;Mitchell E. Horwitz ,&nbsp;Gwynn Long ,&nbsp;Richard Lopez ,&nbsp;David A. Rizzieri ,&nbsp;Stefanie Sarantopoulos ,&nbsp;Keith M. Sullivan ,&nbsp;Nelson J. Chao ,&nbsp;Anthony D. Sung","doi":"10.1016/j.bbmt.2020.09.016","DOIUrl":"10.1016/j.bbmt.2020.09.016","url":null,"abstract":"<div><p>Delirium is common among adults undergoing hematopoietic stem cell transplantation (HCT), although the clinical and neuroimaging correlates of post-HCT delirium have not been adequately delineated. We therefore examined the frequency of delirium and neuroimaging correlates of post-transplant delirium in a retrospective cohort of 115 adults undergoing neuroimaging after allogeneic HCT. Delirium was established using previously validated methods for retrospective identification of chart-assessed postprocedural delirium. Chart reviews were independently conducted by a multidisciplinary team with expertise in HCT, psychiatry, and psychology on consecutive allogeneic HCT patients who underwent neuroimaging assessments and transplantation at a single center between January 2009 and December 2016. Neuroimaging markers of white matter damage and brain volume loss were also recorded. In total, 115 patients were included, ranging in age from 20 to 74 years (mean [SD] age, 49 [13]). Fifty-three patients (46%) developed post-HCT delirium. In an adjusted model, delirium incidence was associated with older age (odds ratio [OR], 1.92 [1.28, 2.87] per decade, <em>P</em> = .002), greater severity of white matter hyperintensities (OR, 1.95 [1.06, 3.57], <em>P</em> = .031), and conditioning intensity (OR, 6.37 [2.20, 18.45], <em>P</em> &lt; .001) but was unrelated to cortical atrophy (<em>P</em> = .777). Delirium was associated with fewer hospital-free days (<em>P</em> = .023) but was not associated with overall survival (hazard ratio, 0.95 [0.56, 1.61], <em>P</em> = .844). Greater incidence of delirium following HCT was associated with greater age, microvascular burden, and conditioning intensity. Pre-HCT consideration of microvascular burden and other neuroimaging biomarkers of risk may be warranted.</p></div>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"26 12","pages":"Pages 2323-2328"},"PeriodicalIF":4.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbmt.2020.09.016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38503565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical-Grade Expanded Regulatory T Cells Are Enriched with Highly Suppressive Cells Producing IL-10, Granzyme B, and IL-35","authors":"Francesca Ulbar ,&nbsp;Ida Villanova ,&nbsp;Raffaella Giancola ,&nbsp;Stefano Baldoni ,&nbsp;Francesco Guardalupi ,&nbsp;Bianca Fabi ,&nbsp;Paola Olioso ,&nbsp;Anita Capone ,&nbsp;Rosaria Sola ,&nbsp;Sara Ciardelli ,&nbsp;Beatrice Del Papa ,&nbsp;Antonello Brattelli ,&nbsp;Ilda Ricciardi ,&nbsp;Stefano Taricani ,&nbsp;Giulia Sabbatinelli ,&nbsp;Ornella Iuliani ,&nbsp;Cecilia Passeri ,&nbsp;Paolo Sportoletti ,&nbsp;Stella Santarone ,&nbsp;Antonio Pierini ,&nbsp;Mauro Di Ianni","doi":"10.1016/j.bbmt.2020.08.034","DOIUrl":"10.1016/j.bbmt.2020.08.034","url":null,"abstract":"<div><p>In the setting of T cell-depleted, full-haplotype mismatched transplantation, adoptive immunotherapy with regulatory T cells (Tregs) and conventional T cells (Tcons) can prevent graft-versus-host disease (GVHD) and improve post-transplantation immunologic reconstitution and is associated with a powerful graft-versus-leukemia effect. To improve the purity and the quantity of the infused Tregs, good manufacturing practices (GMP)-compatible expansion protocols are needed. Here we expanded Tregs using an automated, clinical-grade protocol. Cells were extensively characterized in vitro, and their efficiency was tested in vivo in a mouse model. Tregs were selected by CliniMacs (CD4⁺CD25⁺, 94.5 ± 6.3%; FoxP3⁺, 63.7 ± 11.5%; CD127⁺, 20 ± 3%; suppressive activity, 60 ± 7%), and an aliquot of 100 × 10⁶ was expanded for 14 days using the CliniMACS Prodigy System, obtaining 684 ± 279 × 10⁶ cells (CD4⁺CD25⁺, 99.6 ± 0.2%; FoxP3⁺, 82 ± 8%; CD127⁺, 1.1 ± 0.8%; suppressive activity, 75 ± 12%). CD39 and CTLA4 expression levels increased from 22.4 ± 12% to 58.1 ± 13.3% (<em>P</em> &lt; .05) and from 20.4 ± 6.7% to 85.4 ± 9.8% (<em>P</em> &lt; .01), respectively. TIM3 levels increased from .4 ± .05% to 29 ± 16% (<em>P</em> &lt; .05). Memory Tregs were the prevalent population, whereas naive Tregs almost disappeared at the end of the culture. mRNA analysis displayed significant increases in CD39, IL-10, granzyme B, and IL-35 levels at the end of culture period (<em>P</em> &lt; .05). Conversely, IFNγ expression decreased significantly by day +14. Expanded Tregs were sorted according to TIM3, CD39, and CD62L expression levels (purity &gt;95%). When sorted populations were analyzed, TIM3⁺ cells showed significant increases in IL-10 and granzyme B (<em>P</em> &lt; .01) .When expanded Tregs were infused in an NSG murine model, mice that received Tcons only died of GVHD, whereas mice that received both Tcons and Tregs survived without GVHD. GMP grade expanded cells that display phenotypic and functional Treg characteristics can be obtained using a fully automated system. Treg suppression is mediated by multiple overlapping mechanisms (eg, CTLA-4, CD39, IL-10, IL-35, TGF-β, granzyme B). TIM3⁺ cells emerge as a potentially highly suppressive population.</p><p>© 2020 American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc.</p></div>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"26 12","pages":"Pages 2204-2210"},"PeriodicalIF":4.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbmt.2020.08.034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38406253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes with Autologous or Allogeneic Stem Cell Transplantation in Patients with Plasma Cell Leukemia in the Era of Novel Agents","authors":"Christopher Lemieux,&nbsp;Laura J. Johnston,&nbsp;Robert Lowsky,&nbsp;Lori S. Muffly,&nbsp;Juliana K. Craig,&nbsp;Parveen Shiraz,&nbsp;Andrew Rezvani,&nbsp;Matthew J. Frank,&nbsp;Wen-Kai Weng,&nbsp;Everett Meyer,&nbsp;Judith Shizuru,&nbsp;Sally Arai,&nbsp;Robert Negrin,&nbsp;David B. Miklos,&nbsp;Surbhi Sidana","doi":"10.1016/j.bbmt.2020.08.035","DOIUrl":"10.1016/j.bbmt.2020.08.035","url":null,"abstract":"<div><p>Plasma cell leukemia (PCL) is a rare and very aggressive plasma cell disorder. The optimal treatment approach, including whether to pursue an autologous (auto) or allogeneic (allo) stem cell transplantation (SCT) is not clear, given the lack of clinical trial-based evidence. This single-center retrospective study describes the outcomes of 16 patients with PCL (n = 14 with primary PCL) who underwent either autoSCT (n = 9) or alloSCT (n = 7) for PCL in the era of novel agents, between 2007 and 2019. The median age of the cohort was 58 years. High-risk cytogenetics were found in 50% of the patients. All patients received a proteasome inhibitor and/or immunomodulatory drug-based regimen before transplantation. At the time of transplantation, 10 patients (62%) obtained at least a very good partial response (VGPR). The response after autoSCT (3 months) was at least a VGPR in 6 patients (67%; complete response [CR] in 5). All patients undergoing alloSCT achieved a CR at 3 months. Maintenance therapy was provided to 5 patients (56%) after autoSCT. The median progression-free survival after transplantation was 6 months in the autoSCT group, compared with 18 months in the alloSCT group (<em>P</em> = .09), and median overall survival (OS) after transplantation in the 2 groups was 19 months and 40 months, respectively (<em>P</em> = .41). The median OS from diagnosis was 27 months and 49 months, respectively (<em>P</em> = .50). Of the 11 deaths, 10 patients (91%) died of relapsed disease. AlloSCT was not observed to offer any significant survival advantage over autoSCT in PCL, in agreement with recent reports, and relapse remains the primary cause of death in these patients.</p></div>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"26 12","pages":"Pages e328-e332"},"PeriodicalIF":4.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbmt.2020.08.035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38406255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines for Cord Blood Unit Selection","authors":"Ioannis Politikos ,&nbsp;Eric Davis ,&nbsp;Melissa Nhaissi ,&nbsp;John E. Wagner ,&nbsp;Claudio G. Brunstein ,&nbsp;Sandra Cohen ,&nbsp;Elizabeth J. Shpall ,&nbsp;Filippo Milano ,&nbsp;Andromachi Scaradavou ,&nbsp;Juliet N. Barker ,&nbsp;American Society for Transplantation and Cellular Therapy Cord Blood Special Interest Group","doi":"10.1016/j.bbmt.2020.07.030","DOIUrl":"10.1016/j.bbmt.2020.07.030","url":null,"abstract":"<div><p>Optimal cord blood (CB) unit selection is critical to maximize the likelihood of successful engraftment and survival after CB transplantation (CBT). However, unit selection can be complex because multiple characteristics must be considered including unit cell dose, donor-recipient human leukocyte antigen (HLA) match, and unit quality. This review provides evidence-based and experience-based comprehensive guidelines for CB unit selection. Topics addressed include the use of both the TNC and the CD34⁺ cell dose, as well as the CD34⁺ cell to TNC content ratio to evaluate unit progenitor cell content and engraftment potential, the acceptable TNC and CD34⁺ cell dose criteria that define an adequate single-unit graft, and the indication and acceptable cell dose criteria for double-unit grafts. The acceptable criteria for 6-loci (HLA-A, -B antigen, -DRB1 allele) and 8-allele (HLA-A, -B, -C, -DRB1) donor-recipient HLA match, the evaluation of patients with donor-specific HLA antibodies, and the multiple determinants of unit quality are also reviewed in detail. Finally, a practical step-by-step guide to CB searches and the principles that guide ultimate graft selection are outlined.</p></div>","PeriodicalId":9165,"journal":{"name":"Biology of Blood and Marrow Transplantation","volume":"26 12","pages":"Pages 2190-2196"},"PeriodicalIF":4.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbmt.2020.07.030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38212626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}