ISRN hepatology最新文献_第2页

Overexpression of Regulatory T Cells Type 1 (Tr1) Specific Markers in a Patient with HCV-Induced Hepatocellular Carcinoma. 调节性T细胞1型(Tr1)特异性标志物在hcv诱导的肝细胞癌患者中的过表达

ISRN hepatology Pub Date : 2013-10-23 eCollection Date: 2013-01-01 DOI: 10.1155/2013/928485

Laurissa Ouaguia, Olivier Morales, Dhafer Mrizak, Khaldoun Ghazal, Emmanuel Boleslawski, Claude Auriault, Véronique Pancré, Yvan de Launoit, Filoména Conti, Nadira Delhem

{"title":"Overexpression of Regulatory T Cells Type 1 (Tr1) Specific Markers in a Patient with HCV-Induced Hepatocellular Carcinoma.","authors":"Laurissa Ouaguia, Olivier Morales, Dhafer Mrizak, Khaldoun Ghazal, Emmanuel Boleslawski, Claude Auriault, Véronique Pancré, Yvan de Launoit, Filoména Conti, Nadira Delhem","doi":"10.1155/2013/928485","DOIUrl":"https://doi.org/10.1155/2013/928485","url":null,"abstract":"Hepatitis C virus (HCV) is an important causative agent of liver disease, but factors that determine the resolution or progression of infection are poorly understood. In this study, we suggested that existence of immunosuppressive mechanisms, supported by regulatory T cells and especially the regulatory T cell 1 subset (Tr1), may explain the impaired immune response during infection and thus the fibrosis aggravation to hepatocellular carcinoma (HCC). Using quantitative real-time PCR, we investigated the intra-hepatic presence of Tr1 cells in biopsies from a genotype 1b infected patient followed for an 18-year period from cirrhosis to HCC. We described a significant increase of gene expression in particular for the cytokines IL-10, TGF-β, and their receptors that were perfectly correlated with an increased expression of the Tr1 specific markers (combined expression of CD4, CD18, and CD49b). This was strongly marked since the patient evolved in the pathology and could explain the failure of the treatment. In conclusion, evidence of regulatory T cell installation in the liver of chronically infected patient with cirrhosis and HCC suggests for the first time a key role for these cells in the course of HCV infection. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"928485"},"PeriodicalIF":0.0,"publicationDate":"2013-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/928485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34603976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Roles of Vitamin A Metabolism in the Development of Hepatic Insulin Resistance. 维生素 A 代谢在肝脏胰岛素抵抗发展过程中的作用

ISRN hepatology Pub Date : 2013-09-30 eCollection Date: 2013-01-01 DOI: 10.1155/2013/534972

Guoxun Chen

{"title":"Roles of Vitamin A Metabolism in the Development of Hepatic Insulin Resistance.","authors":"Guoxun Chen","doi":"10.1155/2013/534972","DOIUrl":"10.1155/2013/534972","url":null,"abstract":"The increase in the number of people with obesity- and noninsulin-dependent diabetes mellitus has become a major public health concern. Insulin resistance is a common feature closely associated with human obesity and diabetes. Insulin regulates metabolism, at least in part, via the control of the expression of the hepatic genes involved in glucose and fatty acid metabolism. Insulin resistance is always associated with profound changes of the expression of hepatic genes for glucose and lipid metabolism. As an essential micronutrient, vitamin A (VA) is needed in a variety of physiological functions. The active metablite of VA, retinoic acid (RA), regulates the expression of genes through the activation of transcription factors bound to the RA-responsive elements in the promoters of RA-targeted genes. Recently, retinoids have been proposed to play roles in glucose and lipid metabolism and energy homeostasis. This paper summarizes the recent progresses in our understanding of VA metabolism in the liver and of the potential transcription factors mediating RA responses. These transcription factors are the retinoic acid receptor, the retinoid X receptor, the hepatocyte nuclear factor 4α, the chicken ovalbumin upstream promoter-transcription factor II, and the peroxisome proliferator-activated receptor β/δ. This paper also summarizes the effects of VA status and RA treatments on the glucose and lipid metabolism in vivo and the effects of retinoid treatments on the expression of insulin-regulated genes involved in the glucose and fatty acid metabolism in the primary hepatocytes. I discuss the roles of RA production in the development of insulin resistance in hepatocytes and proposes a mechanism by which RA production may contribute to hepatic insulin resistance. Given the large amount of information and progresses regarding the physiological functions of VA, this paper mainly focuses on the findings in the liver and hepatocytes and only mentions the relative findings in other tissues and cells. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"534972"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34603969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NT-proBNP Changes in Patients with Ascites during Large Volume Paracentesis. 腹水患者在大容量腹腔穿刺过程中的 NT-proBNP 变化

ISRN hepatology Pub Date : 2013-09-22 eCollection Date: 2013-01-01 DOI: 10.1155/2013/959474

Vi Nguyen, Rob Zielinski, Paul Harnett, Katherine Miller, Henry Chan, Nikitha Vootakuru, Priya Acharya, Montaha Khan, Oliver Gibbs, Sarika Gupta, Anjla Devi, Shani Phillips, Jacob George, David van der Poorten

{"title":"NT-proBNP Changes in Patients with Ascites during Large Volume Paracentesis.","authors":"Vi Nguyen, Rob Zielinski, Paul Harnett, Katherine Miller, Henry Chan, Nikitha Vootakuru, Priya Acharya, Montaha Khan, Oliver Gibbs, Sarika Gupta, Anjla Devi, Shani Phillips, Jacob George, David van der Poorten","doi":"10.1155/2013/959474","DOIUrl":"10.1155/2013/959474","url":null,"abstract":"Background. N-terminal probrain natriuretic peptide (NT-proBNP) is a hormone involved in the regulation of cardiovascular homeostasis. Changes in serum NT-proBNP during large volume paracentesis (LVP) in patients with ascites have never before been examined. Aims. To determine if significant changes in serum NT-proBNP occur in patients undergoing LVP and the associated clinical correlates in patients with cirrhosis. Method. A total of 45 patients with ascites were prospectively recruited. Serum NT-proBNP, biochemistry, and haemodynamics were determined at baseline and at key time points during and after paracentesis. Results. 34 patients were analysed; 19 had ascites due to cirrhosis and 15 from malignancy. In those with cirrhosis, NT-proBNP decreased by 77.3 pg/mL at 2 L of drainage and 94.3 pg/mL at the end of paracentesis, compared with an increase of 10.5 pg/mL and 77.2 pg/mL in cancer patients at the same time points (P = 0.05 and P = 0.03). Only congestive cardiac failure (CCF) was an independent predictor of significant NT-proBNP changes at the end of drainage in cirrhotic patients (P < 0.01). There were no significant changes in haemodynamics or renal biochemistry for either group. Conclusion. Significant reductions in serum NT-proBNP during LVP occur in patients with cirrhosis but not malignancy, and only comorbid CCF appeared to predict such changes. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"959474"},"PeriodicalIF":0.0,"publicationDate":"2013-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34604987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Levels of Interleukin-6 and Interleukin-10 as Biomarkers for Hepatocellular Carcinoma in Egyptian Patients. 血清白细胞介素-6 和白细胞介素-10 水平作为埃及患者肝细胞癌的生物标志物

ISRN hepatology Pub Date : 2013-09-15 eCollection Date: 2013-01-01 DOI: 10.1155/2013/412317

Mohamed S Othman, Ahmed M Aref, Amal A Mohamed, Wesam A Ibrahim

{"title":"Serum Levels of Interleukin-6 and Interleukin-10 as Biomarkers for Hepatocellular Carcinoma in Egyptian Patients.","authors":"Mohamed S Othman, Ahmed M Aref, Amal A Mohamed, Wesam A Ibrahim","doi":"10.1155/2013/412317","DOIUrl":"10.1155/2013/412317","url":null,"abstract":"Interleukin-10 (IL-10) and interleukin-6 (IL-6) have been reported to be related to hepatocellular carcinoma (HCC) prognosis. This study aimed to investigate the clinical usefulness of serum levels of IL-6 and IL-10 as biomarkers for HCC among high-risk patients. Materials and Methods. 80 individuals were enrolled in this study; they were categorized into 4 groups: group 1 healthy individuals (NC) (n = 20), group 2 chronic hepatitis C virus (HCV) patients (n = 20), group 3 cirrhotic patients (LC) (n = 20), and HCC group (n = 20). Using ELISA technique serum levels of IL-6, IL-10, and alpha fetoprotein (AFP) were evaluated in all groups. Results. The mean serum levels of IL-6 were significantly higher in HCC than in LC, HCV, and NC groups (13.99 ± 1.80, 7.49 ± 0.43, 5.78 ± 0.74, and 2.57 ± 0.31), respectively (P < 0.05); also the serum levels of IL-10 were significantly higher in HCC compared with LC, HCV, and NC groups (13.69 ± 1.89, 7.37 ± 0.53, 5.18 ± 0.6, and 3.31 ± 0.42) (P < 0.05). We also found that the tumor size is correlated strongly with IL-6 and IL-10 levels (r = 0.925, P < 0.001; r = 0.821, P < 0.001), respectively. Conclusion. The combination of those markers may help to identify a group of HCC patients with low AFP. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"412317"},"PeriodicalIF":0.0,"publicationDate":"2013-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34669734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absence of Hepatitis B Resistance Mutants before Introduction of Oral Antiviral Therapy. 引入口服抗病毒治疗前乙型肝炎耐药突变体的缺失。

ISRN hepatology Pub Date : 2013-09-12 eCollection Date: 2013-01-01 DOI: 10.1155/2013/130384

Martin Moehlen, Maria De Medina, Mary Hill, Lennox Jeffers, Eugene R Schiff, Paul Martin

{"title":"Absence of Hepatitis B Resistance Mutants before Introduction of Oral Antiviral Therapy.","authors":"Martin Moehlen, Maria De Medina, Mary Hill, Lennox Jeffers, Eugene R Schiff, Paul Martin","doi":"10.1155/2013/130384","DOIUrl":"https://doi.org/10.1155/2013/130384","url":null,"abstract":"Introduction. The aim of this study was to assess whether hepatitis B virus drug resistant mutations antedated the widespread use of nucleos(t)ide analogues in treatment naïve patients. A number of reports have suggested that drug resistant mutants can be detected in apparently treatment naïve patients. Study. Fifty deidentified serum samples collected from 1986 to 1992 from patients with replicative chronic HBV infection at the University of Miami were genotyped and tested for resistance mutations using a line probe assay InnoLiPA HBV DR v2/v3. Serum HBV DNA was measured. All patients had documented chronic HBV infection with a detectable viral load, HBeAg seropositivity, and absence of HIV infection. Results. Of the 50 individuals included, 86% were male, mean age was 40 ± 12 years, and mostly genotype A. The mean HBV DNA was 126 pg/mL (range 6.4 to 557.0). No mutations were identified. Conclusions. The absence of drug induced mutations in these sera collected several years prior to the introduction of oral antiviral therapy suggests that these mutations do not occur in treatment naïve populations. Detection of drug resistance in an apparently treatment naïve subject suggests either unrecognized prior antiviral therapy or infection by an inoculum from a treatment experienced patient. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"130384"},"PeriodicalIF":0.0,"publicationDate":"2013-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34669730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Selective Arterial Embolization of Liver Metastases from Gastrinomas: A Single-Centre Experience. 选择性动脉栓塞治疗胃原质瘤肝转移:单中心经验。

ISRN hepatology Pub Date : 2013-07-29 eCollection Date: 2013-01-01 DOI: 10.1155/2013/174608

Anneke P J Jilesen, Heinz Josef Klümpen, Olivier R C Busch, T M van Gulik, Krijn P van Lienden, Dirk J Gouma, Els J M Nieveen van Dijkum

{"title":"Selective Arterial Embolization of Liver Metastases from Gastrinomas: A Single-Centre Experience.","authors":"Anneke P J Jilesen, Heinz Josef Klümpen, Olivier R C Busch, T M van Gulik, Krijn P van Lienden, Dirk J Gouma, Els J M Nieveen van Dijkum","doi":"10.1155/2013/174608","DOIUrl":"https://doi.org/10.1155/2013/174608","url":null,"abstract":"Background. Gastrinomas are rare functional neuroendocrine tumors causing the Zollinger-Ellison syndrome (ZES). At presentation, up to 25% of gastrinomas are metastasized, predominantly to the liver. Embolization of liver metastases might reduce symptoms of ZES although a postembolization syndrome can occur. In this study, the results of embolization are presented, and the literature results are described. Methods. From a prospective database of pancreatic neuroendocrine tumors, all patients with liver metastatic gastrinomas were selected if treated with arterial embolization. Primary outcome parameters were symptom reduction, complications, and response rate. The literature search was performed with these items. Results. Three patients were identified; two presented with synchronous liver metastases. All the three patients had symptoms of ZES before embolization. Postembolization syndrome occurred in two patients. Six months after embolization, all the 3 patients had a clinical and complete radiological response; a biochemical response was seen in 2/3 patients. From the literature, only a small number of gastrinoma patients treated with liver embolization for liver metastases were found, and similar results were described. Conclusion. Selective liver embolization is an effective and safe therapy for the treatment of liver metastatic gastrinomas in the reduction of ZES. Individual treatment strategies must be made for the optimal success rate. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"174608"},"PeriodicalIF":0.0,"publicationDate":"2013-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/174608","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34669732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastrointestinal Bleeding in Cirrhotic Patients with Portal Hypertension. 肝硬化门静脉高压症患者的消化道出血。

ISRN hepatology Pub Date : 2013-07-22 eCollection Date: 2013-01-01 DOI: 10.1155/2013/541836

Erwin Biecker

{"title":"Gastrointestinal Bleeding in Cirrhotic Patients with Portal Hypertension.","authors":"Erwin Biecker","doi":"10.1155/2013/541836","DOIUrl":"https://doi.org/10.1155/2013/541836","url":null,"abstract":"Gastrointestinal bleeding related to portal hypertension is a serious complication in patients with liver cirrhosis. Most patients bleed from esophageal or gastric varices, but bleeding from ectopic varices or portal hypertensive gastropathy is also possible. The management of acute bleeding has changed over the last years. Patients are managed with a combination of endoscopic and pharmacologic treatment. The endoscopic treatment of choice for esophageal variceal bleeding is variceal band ligation. Bleeding from gastric varices is treated by injection with cyanoacrylate. Treatment with vasoactive drugs as well as antibiotic treatment is started before or at the time point of endoscopy. The first-line treatment for primary prophylaxis of esophageal variceal bleeding is nonselective beta blockers. Pharmacologic therapy is recommended for most patients; band ligation is an alternative in patients with contraindications for or intolerability of beta blockers. Treatment options for secondary prophylaxis include variceal band ligation, beta blockers, a combination of nitrates and beta blockers, and combination of band ligation and pharmacologic treatment. A clear superiority of one treatment over the other has not been shown. Bleeding from portal hypertensive gastropathy or ectopic varices is less common. Treatment options include beta blocker therapy, injection therapy, and interventional radiology. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"541836"},"PeriodicalIF":0.0,"publicationDate":"2013-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/541836","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34603972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

In Vivo Effect of Arsenic Trioxide on Keap1-p62-Nrf2 Signaling Pathway in Mouse Liver: Expression of Antioxidant Responsive Element-Driven Genes Related to Glutathione Metabolism. 三氧化二砷对小鼠肝脏中 Keap1-p62-Nrf2 信号通路的体内效应：与谷胱甘肽代谢相关的抗氧化反应元件驱动基因的表达。

ISRN hepatology Pub Date : 2013-07-10 eCollection Date: 2013-01-01 DOI: 10.1155/2013/817693

Ritu Srivastava, Archya Sengupta, Sandip Mukherjee, Sarmishtha Chatterjee, Muthammal Sudarshan, Anindita Chakraborty, Shelley Bhattacharya, Ansuman Chattopadhyay

{"title":"In Vivo Effect of Arsenic Trioxide on Keap1-p62-Nrf2 Signaling Pathway in Mouse Liver: Expression of Antioxidant Responsive Element-Driven Genes Related to Glutathione Metabolism.","authors":"Ritu Srivastava, Archya Sengupta, Sandip Mukherjee, Sarmishtha Chatterjee, Muthammal Sudarshan, Anindita Chakraborty, Shelley Bhattacharya, Ansuman Chattopadhyay","doi":"10.1155/2013/817693","DOIUrl":"10.1155/2013/817693","url":null,"abstract":"Arsenic is a Group I human carcinogen, and chronic arsenic exposure through drinking water is a major threat to human population. Liver is one of the major organs for the detoxification of arsenic. The present study was carried out in mice in vivo after arsenic treatment through drinking water at different doses and time of exposure. Arsenic toxicity is found to be mediated by reactive oxygen species. Nuclear factor (erythroid-2 related) factor 2 (Nrf2)/Keap1 (Kelch-like ECH-associated protein 1)/ARE (antioxidant response element)-driven target gene system protects cells against oxidative stress and maintains cellular oxidative homeostasis. Our result showed 0.4 ppm, 2 ppm, and 4 ppm arsenic trioxide treatment through drinking water for 30 days and 90 days induced damages in the liver of Swiss albino mice as evidenced by histopathology, disturbances in liver function, induction of heat shock protein 70, modulation of trace elements, alteration in reduced glutathione level, glutathione-s-transferase and catalase activity, malondialdehyde production, and induction of apoptosis. Cellular Nrf2 protein level and mRNA level increased in all treatment groups. Keap1 protein as well as mRNA level decreased concomitantly in arsenic treated mice. Our study clearly indicates the important role of Nrf2 in activating ARE driven genes related to GSH metabolic pathway and also the adaptive response mechanisms in arsenic induced hepatotoxicity. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"817693"},"PeriodicalIF":0.0,"publicationDate":"2013-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34603975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Female Genital Cutting and Hepatitis C Spread in Egypt. 女性生殖器切割和丙型肝炎在埃及传播。

ISRN hepatology Pub Date : 2013-05-07 eCollection Date: 2013-01-01 DOI: 10.1155/2013/617480

Chris Kenyon, Jozefien Buyze, Ludwig Apers, Robert Colebunders

引用次数: 4

Establishment and Validation of an Orthotopic Metastatic Mouse Model of Colorectal Cancer. 结直肠癌原位转移小鼠模型的建立与验证。

ISRN hepatology Pub Date : 2013-04-21 eCollection Date: 2013-01-01 DOI: 10.1155/2013/206875

Ashwani Rajput, Ekta Agarwal, Premila Leiphrakpam, Michael G Brattain, Sanjib Chowdhury

{"title":"Establishment and Validation of an Orthotopic Metastatic Mouse Model of Colorectal Cancer.","authors":"Ashwani Rajput, Ekta Agarwal, Premila Leiphrakpam, Michael G Brattain, Sanjib Chowdhury","doi":"10.1155/2013/206875","DOIUrl":"https://doi.org/10.1155/2013/206875","url":null,"abstract":"Metastases are largely responsible for cancer deaths in solid tumors due to the lack of effective therapies against disseminated disease, and there is an urgent need to fill this gap. This study demonstrates an orthotopic colorectal cancer (CRC) mouse model system to develop spontaneous metastasis in vivo and compare its reproducibility against human CRC. IGF1R-dependent GEO human CRC cells were used to study metastatic colonization using orthotopic transplantation procedures and demonstrated robust liver metastasis. Cell proliferation assays were performed both in the orthotopic primary colon and liver metastatic tumors, and human CRC patient's specimen and similar patterns in H&E and Ki67 staining were observed between the orthotopically generated primary and liver metastatic tumors and human CRC specimens. Microarray analysis was performed to generate gene signatures, compared with deposited human CRC gene expression data sets, analyzed by Oncomine, and revealed similarity in gene signatures with increased aggressive markers expression associated with CRC in orthotopically generated liver metastasis. Thus, we have developed an orthotopic mouse model that reproduces human CRC metastasis. This model system can be effective in developing new therapeutic strategies against disseminated disease and could be implemented for identifying genes that regulate the development and/or maintenance of established metastasis. ","PeriodicalId":91521,"journal":{"name":"ISRN hepatology","volume":"2013 ","pages":"206875"},"PeriodicalIF":0.0,"publicationDate":"2013-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/206875","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34497803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17