Journal of microbiology & experimentation最新文献

{"title":"Paleontology, stratigraphy, paleoenvironment and paleogeography of the seventy Tethyan Maastrichtian-Paleogene foraminiferal species of Anan, a review","authors":"H. Anan","doi":"10.15406/jmen.2021.09.00329","DOIUrl":"https://doi.org/10.15406/jmen.2021.09.00329","url":null,"abstract":"","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45321772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhodococcus erythropolis prosthetic valve infective endocarditis: case report","authors":"F. Nacinovich, A. Fiori, P FernandezOses, S. Castillo, M. Pennini, M. Merkt, I. Chapman, J. Costabel, R. Ronderos, F. Piccinini, D. Navia","doi":"10.15406/jmen.2021.09.00328","DOIUrl":"https://doi.org/10.15406/jmen.2021.09.00328","url":null,"abstract":"","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43184413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of ectoparasites and gastrointestinal endoparasites and body condition in sheep from four farms of the municipality of Valledupar, Cesar","authors":"Bolano Narvaez Yessika Paola, Canate Gonzalez Abid Silvestre, Herrera Demares Patricia, Bernal Diaz Ana Karina","doi":"10.15406/JMEN.2021.09.00325","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00325","url":null,"abstract":"The current study was carried out to evaluate the association between the presence of gastrointestinal ectoparasites and endoparasites with the body condition in sheep sampled in 4 farms located in the municipality of Valledupar, Cesar. The type of study is descriptive, prospective and cross-sectional. Samples were taken from 111 sheep without exclusion of sex distributed in 4 farms in the municipality of Valledupar. The McMaster technique was used to determine the parasite loads of the sheep, later stool cultures were made to the samples that presented high parasite loads, the Baerman technique was applied in order to identify the parasites present in the samples and it was evaluated if there is a relationship between body condition/anemic state, body condition / parasite load and anemic state/ parasite load using the R statistical software and the Chi-square test as hypothesis test. The results show that the prevalence in this research was 84.6% of which 22.5% of the parasitized sheep had high loads, presenting a higher prevalence of the gastrointestinal endoparasite Haemonchus sp with a percentage of 9.9% (11/25), followed by Cooperia with 6.3% (7/25), Ostertagia sp 2.7% (3/25), Trichuris sp 0.9% (1/25) Strongyloides sp 0.9% (1/25), Eimeria 0.9% (1/25) and finally Trichostrongylus with a percentage 0.9% (1/25) for a total of 22.5% and biparasitism did not occur. No ectoparasites were found in sheep. The association between the body anemic status of the sheep determined by the Famacha technique and the presence of gastrointestinal parasites was p<0.05; while the relationship between the body condition in sheep and the parasites presented in them was p>0.05. In conclusion, it is established that if there is an association between Famacha and the presence of gastrointestinal endoparasites, the association between body condition and ovine parasites is not established, taking into account that Famacha turned out to be a useful technique to determine in which anemic state and which the body condition is not associated with the parasite load and is responsible for the fact that this technique is subjective and that therefore the decrease in the body mass and weight of the sheep may be due to various factors and not necessarily to the presence of gastrointestinal endoparasites and ectoparasites , considering that the latter were absent in this research.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43782192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of syphilis antibodies among blood donors at North Darfur State–Sudan, from 2017 to 2019","authors":"M. A. Ibrahim, N. Adam, M. A. Adam, N. Abdelrahman, Marawa Ahmed Mohammed Abd Erahman","doi":"10.15406/JMEN.2021.09.00322","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00322","url":null,"abstract":"Background: syphilis is one of the infections transmitted through blood transfusion as shown by high antibodies detection in healthy African blood. Syphilis prevalence among blood donors in sub-Saharan African countries varies among people; In Nigeria, Mali, Tanzania, and Kenya the prevalence was 3.1%, 0.3%, 12.8%, and 3.8% respectively. This study aimed to determine the seroprevalence of syphilis antibodies among blood donors at North Darfur State – Sudan, from 2017 to 2019. Methods: This retrospective descriptive analysis of consecutive blood donor’s data records covering the period from January 2017 to December 2019 was performed. The medical and socio-demographic histories of the donors were registered in the logbook. The data was analyzed using IBM SPSS Statistical package version 20. Prevalence of syphilis was expressed as the number of sero-positive samples per year. Pearson Chi-squire (χ2) test was used to evaluate the relationship between categorical variants. Ethical approval was obtained from Al Fashir University and Sudan Ministry of Health Ethical Review Boards. Results: A total of 14819 blood donors were analyzed. The overall sero-prevalence of syphilis antibodies between 2017 and 2019 were 1927 (13 %). They were all adult’s male, aged between 17 to 64 years, with a median age 29.5 years. The seroprevalence of syphilis was 10.9 % in 2017; increased to 13 % in 2018 and subsequently increased to 14.8 % in 2019. Conclusion: In This study it was observed that the seroprevalence of syphilis among blood donors continues high during the study period.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43212574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of storage container on the bacteriological quality of water from different sources","authors":"Osuji Malachy Ikeokwu","doi":"10.15406/JMEN.2021.09.00323","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00323","url":null,"abstract":"This research work was carried out to measure the effect of storage container on the bacterial population of water from different sources over a long storage time. Spread plate and other bacteriological techniques were adopted using Nutrient (NA), Salmonella/ Shigella(SSA), MacConkey(MAC), and Thiosulphite citrate bile sucrose (TCBS) agar. Calabash, Glass, Metal and Plastic containers were used to store rain, river, and tap water samples. Sample analysis was taken at time interval of week 0, 1, 3, 5, and 14. In rainwater stored in calabash, total heterotrophic bioload decreased from 9.2 logcfu/ml at week 0 to 5.3 log cfu/ml at week 5 and steady thereafter. Other bioloads in rain water stored in other containers showed similar trend in growth pattern. For river water stored in calabash container, total heterotrophic bioload decreased all through. For river water stored in glass container, it also decreased from 7.0 logcfu/ml at week 0 to 5.8 logcfu/ml at week 1 and was steady thereafter. Other bioloads in river water stored in other containers decreased from week 0 to week 14 at various concentrations. In tap water stored in glass and other containers, all decreased throughout. Several other factors were discovered in this research work to be responsible for the bioload population decrease observed. They include toxins produced during stationary phase as secondary metabolites, acids, nutrient depletion, pH variation, temperature changes. On the statistical analysis (ANOVA), it showed that the containers have significant effect on the bioload of the stored water after 14 weeks storage as plastic recorded the highest bioload change. To this effect people are advised to use plastic container for water storage for a long period. Also people are to filter the water to remove biofilms formed and debris of bacteria that died due to toxins produced. Nutrient depletion and effect of other factors contributed in bacterial population decrease.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46605042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine fungi from different habitats recorded from 2001 to date in Mexico","authors":"A. Portillo-López, Sophia González-Martínez","doi":"10.15406/JMEN.2021.09.00320","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00320","url":null,"abstract":"Marine fungi are essential as recyclers of organic matter in the ocean, as well their secondary metabolites are now studied as potential drugs for different diseases. Despite Mexico having an extensive coastline, few resources have been allocated to the research of this group. Through a thorough review of scientific literature between 2001 and February 2021, a systematic listing of marine fungi on Mexico’s marine waters was constructed. In this work, two orders, forty-nine genera, and thirteen species are recorded, of which 50 are new records. The most frequent phylum was Ascomycota (92%; 50 genera), followed Chytridiomycota (4%; 2 genera) and Basidiomycota (4%; 2 genera). Most of them have been reported in the Gulf of Mexico, followed by the Pacific Ocean, Gulf of California and, the Caribbean. A new halophile species isolated from deep sediment in the Gulf of California (Aspergillus loretoensis) is also reported.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43622125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blastocystis hominis microbiota: study of 13255 patients and review of the literature ","authors":"J. Belkhair, I. Karrati, M. Tarmidi, M. Mezouari, R. Moutaj","doi":"10.15406/JMEN.2021.09.00319","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00319","url":null,"abstract":"to objectify the number of subjects infested by this parasite in our population. This is a retrospective study carried out over a period of ten years, from January 2009 until December 2018, in the parasitology-mycology laboratory of the Avicenna military hospital in Marrakech. 13,255 subjects were included with at least one parasitological examination of the stool, of which 2,799 examinations were positive, with a simple parasite index of 21.11%. Blastocystis hominis was found in 675 subjects with a specific parasite index of 5.09%, and it was co-associated in 446 subjects with a specific parasite index (IPSp) of 3.36%. The most frequent association was that of Blastocystis hominis and E. histolytica/dispar with a rate of (25.5%) of all polyparasitic patients followed by the combination of Blastocystis hominis and Entamoeba coli (20.8%), the combination of Blastocystis hominis and Endolimax nana with a rate of (16, 1%) and lastly Blastocystis hominis and Entamoeba hartmani (5.3% In patients with polyparasitism, the association of Blastocystis hominis with Entamoeba coli was 20.8%, with Endolimax nana with a rate of 16.1% and finally with Entamoeba hartmani with a prevalence of 5.3%. It is always necessary to insist on strict observance of the rules of prophylaxis against oral-fecal contamination.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48573028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal effect of chitosan of different molecular weight against Colletotrichum alatae under in vitro conditions","authors":"L. Vásquez, G. Rivera, Y. Miranda, C. Soto, P. Cevallos, I. Rodríguez, R. Couto, D. Crespo","doi":"10.15406/JMEN.2021.09.00316","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00316","url":null,"abstract":"","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":"9 1","pages":"9-13"},"PeriodicalIF":0.0,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42184894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UreC and ZapA virulence genes amplification in clinical specimen of Proteus mirabilis in Bayelsa state, Nigeria","authors":"Itodo Sunday Ewaoche, A. Tolulope, Arikekpar Ibemologi, Ekanem Edmund","doi":"10.15406/JMEN.2021.09.00317","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00317","url":null,"abstract":"Background and objective: Proteus mirabilis is part of the Enterobacteriaceae family, Gram negative bacterium which typically lives in the human gut, which means when it causes illness it can be a serious bloodstream infection, urinary tract infection or disseminated infection. UreC and ZapA virulent genes constitute the major pathogenicity of this organism as well as its resistance to antibiotics. This study was carried out to detect the presence of UreC and ZapA genes in Proteus mirabilis isolates sourced from Federal Medical Centre and Niger Delta University teaching hospital in Bayelsa State. Materials and methods: A total of one hundred and forty (140) clinical samples were collected from Federal Medical Centre (FMC) Yenagoa and Niger Delta University Teaching Hospital (NDUTH) Okolobiri, Bayelsa State. Of the 140 samples collected, 64(45.7%) were from males while 76(54.3%) were from females. The samples were Urine, Sputum, High vaginal swab, Urethral swab, Ear swab and Wound swab. The samples were inoculated in different laboratory media and incubated at 37°C for 48 hours. Morphological, cultural, biochemical characteristics and Polymerase Chain Reaction (PCR) technique were noted appropriately. Means and corresponding standard deviations were calculated for continuous data while proportions, along with the 95% confidence intervals, were calculated for categorical data. Results: A total of 81 bacterial isolates were obtained from these samples, of which 17(20.9%), 22(27.2%), 8(10.0%), 10(12.3%) and 24(29.6%) were Proteus mirabilis , Escherichia coli , Pseudomonas aeruginosa , Klebsiella pneumoniae and Staphylococcus aureus respectively. Fifteen (15) out of the 17(20.9%) P. mirabilis isolates were subjected to single-plex PCR amplification using specific primers after extraction of bacterial DNA from the samples. Out of the 15 samples, 14(93.3%) were positive for UreC gene while 15(100%) were positive to ZapA gene. Conclusion : The present study revealed that virulent genes-UreC and ZapA are highly present in P. mirabilis isolates obtained from clinical specimens from FMC and NDUTH in Bayelsa state, thus making them more pathogenic and resistant to antibiotics curing effect.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46626548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of the storage method of Lactococcus lactis ssp. lactis strains to stabilize their probiotic potential","authors":"D. Novoselova, L. Stoyanova","doi":"10.15406/JMEN.2021.09.00315","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00315","url":null,"abstract":"The object of the study were strains of Lactococcus lactis ssp . lactis isolated from microbiota of fermented milk products brought from Buryatia (Russia), Lebanon, Iran, which have high probiotic potential. The purpose of this work was to optimize the storage method of L. lactis ssp . lactis strains to stabilize their probiotic potential. The various storage methods were used - in skim milk with frequent passages and lyophilization. The cultures were lyophilized on a ‘Krist” installation of the “Betta A “(Germany). Lyophilized strains were restored and their physiological and biochemical properties were studied both immediately after recovery and in a number of passages after storage, their physiological activity was evaluated by the rate of clot formation in skim milk and antimicrobial activity using the microbiological method with the test-cultures and standard antibiotic solutions (Nisaplin, Chloramphenicol, Nystatin). The highest survival rate was found during lyophilization with preliminary treatment. When cultures were restored, the survival rate in the first passage was over 70%, the strains had a high level of antimicrobial activity on both gram-positive and gram-negative bacteria and fungi, including pathogens. The results of the study showed that the most effective method of preserving bacteriocin-forming lactococci is lyophilization using a complex protective environment of the composition (g/l): sucrose-100, gelatin-10, monosodium glutamate-10, sodium citrate-5, which ensures long-term crop life. The introduction of lactococci into the intestinal microbiota leads to the replacement of pathogens and is useful for people with lactase deficiency. Strains enrich the dairy product with biologically active substances, which, in addition to the nutritional effects, have a beneficial effect on health. Lyophilization in combination with pre-treatment of cells contributes to the preservation of their probiotic potential.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45483824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}