Austin journal of analytical and pharmaceutical chemistry最新文献_第3页

Association of Polyphenol Oxidase Activities with Molecular Markers and Yellow Rust Resistance in Diverse Bread Wheat Genotypes 不同面包小麦基因型多酚氧化酶活性与分子标记及抗黄锈性的关系

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-09-03 DOI: 10.26420/austinjanalpharmchem.2021.1134

Asghar Mn, Bajwa Aa, A. A., M. A, S. A., S. Sm

{"title":"Association of Polyphenol Oxidase Activities with Molecular Markers and Yellow Rust Resistance in Diverse Bread Wheat Genotypes","authors":"Asghar Mn, Bajwa Aa, A. A., M. A, S. A., S. Sm","doi":"10.26420/austinjanalpharmchem.2021.1134","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2021.1134","url":null,"abstract":"Polyphenol Oxidase (PPO) catalyses the undesirable browning of wheat products which is of significant concern in consumer acceptance perspectives. Another important yield-limiting cause for wheat crops is wheat rust (e.g., yellow rust), a source of great economic loss worldwide. The purpose of the current research was to screen conventional and synthetic bread wheat genotypes for their PPO activity and yellow rust resistance. Different genotypes differed significantly in total PPO activity and in their activities against different substrates (L-DOPA and Catechol). The synthetically derived bread wheat genotypes 1-279, showed the lowest (39.2 units/min/g) cumulative PPO activity. Ten genotypes each with the highest and lowest PPO activities were selected for testing their association with seven reported molecular markers. Intriguingly the PPO markers reported in literature could not clearly differentiate between contrasting cultivars. Association with yellow rust resistance was also investigated. Interestingly, the rust-resistant genotypes, including 1-263, Ch-43, 1-57 and Emat (all synthetic-derived), exhibited low PPO activity. The current study underpins that there is a need to search for more reliable PPO markers and to further validate association of low PPO activity with yellow rust.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42776708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Chicken Spleen Transfer Factor on Intestinal Mucosa Immunity Barrier of Laying Hens 鸡脾转移因子对蛋鸡肠黏膜免疫屏障的影响

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-08-07 DOI: 10.26420/austinjanalpharmchem.2021.1133

Yu J, M. B, L. J., Chen Y, W. Z, C. J, D. Y

{"title":"The Effect of Chicken Spleen Transfer Factor on Intestinal Mucosa Immunity Barrier of Laying Hens","authors":"Yu J, M. B, L. J., Chen Y, W. Z, C. J, D. Y","doi":"10.26420/austinjanalpharmchem.2021.1133","DOIUrl":"https://doi.org/10.26420/austinjanalpharmchem.2021.1133","url":null,"abstract":"Chicken spleen Transfer Factor (TF) is a low-molecular-weight lymphocyte extract composed of polypeptide and nucleotide. However, its role in regulating intestinal structure and function in laying hens has remained largely unknown. 100 one-day-old laying hens were randomly divided into five groups and administered with different doses of TF (0.00 [control], 0.05mL, 0.10mL, 0.25mL and 1.00mL). The results showed that the high dose of TF (1.00mL) improved the intestinal mucosa morphology and strengthened the digestive and absorption function. Furthermore, the histology analysis revealed an increase in the number of intraepithelial lymphocytes and goblet cells. Similarly, the results from ELISA demonstrated an increase in the content of IL-10 in the intestinal tract, while the content of TNF-a showed a decrease in this regard. The RT-PCR assay also demonstrated the upregulation of the relative mRNA expressions of Muc2, TLR-2, and TLR-4 genes. The intestinal antioxidant function was significantly enhanced. In conclusion, high-dose of TF can improve the intestinal mucosa morphology and structure, enhance digestion and absorption functions, enhance the intestinal mucosal barrier immune function and antioxidant function, and up-regulate Muc2, TLR-2 and TLR-4 gene relative expression.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43641845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Inhibition Effect of Portulaca oleracea against SARS-CoV-2 through Molecular Docking Simulation 马齿苋对SARS-CoV-2抑制作用的分子对接模拟研究

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-05-05 DOI: 10.26420/AUSTINJANALPHARMCHEM.2021.1132

Elizariev An, Zaki Eg, el-Kousy Sm

引用次数: 0

Application of TLC-Spectrodensitometric and Chemometric Methods for Determination of Momenta® Cream: A Comparative Study Applied on Ternary Mixture 薄层色谱法和化学法测定Momenta®乳膏的应用——三元混合物的对比研究

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-03-10 DOI: 10.26420/AUSTINJANALPHARMCHEM.2021.1131

H. Salem, Omar Ma, Derayea Sm, Khalil Aa

引用次数: 0

Development and Validation of HPLC Method for Simultaneous Estimation of Reduced and Oxidized Glutathione in Bulk Pharmaceutical Formulation 高效液相色谱法同时测定原料药中还原型和氧化型谷胱甘肽的开发与验证

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-03-03 DOI: 10.26420/AUSTININTERNMED.2021.1129

N. Singh, Akhtar Mj, A. Anchliya

{"title":"Development and Validation of HPLC Method for Simultaneous Estimation of Reduced and Oxidized Glutathione in Bulk Pharmaceutical Formulation","authors":"N. Singh, Akhtar Mj, A. Anchliya","doi":"10.26420/AUSTININTERNMED.2021.1129","DOIUrl":"https://doi.org/10.26420/AUSTININTERNMED.2021.1129","url":null,"abstract":"The objective of this study was the development, optimization, and validation of a RP-HPLC method for the quantification of reduced glutathione (GSH) and oxidized glutathione (GSSG) in pharmaceutical formulations The separation utilized a C18 column at room temperature with absorption wavelength 210nm. The mobile phase was an isocratic flow of a 95:5 (v/v) mixture of 25mM phosphate buffer (pH 2.7) and methanol with flow rate at 1.0 mL/min. Validation of the method assessed with the methods ability in seven categories: linearity, range, limit of detection, limit of quantification, accuracy, precision, and selectivity. The method show an acceptable degree of linearity with r²=0.9994 and 0.999 over a concentration range of 10-200 μg/mL for GSH and GSSG respectively. The detection limit and quantification limit for GSH 20.7μg/mL and 69.24μg/mL and for GSSG 17.22μg/mL and 57.42μg/mL respectively. The percent recovery of the method was 99.98-100.93 %. Following validation, the method was employed in the determination of glutathione in pharmaceutical formulations in the form of a liposome. The proposed method offers a simple, accurate, and inexpensive way to quantify reduced glutathione.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47797398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Combination of FTIR Spectroscopy and Chemometric Method on Quantitative Approach - A Review FTIR光谱法与化学计量法在定量分析中的结合研究进展

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-03-03 DOI: 10.26420/AUSTININTERNMED.2021.1128

K. Verma, Akhtar Mj, A. Anchliya

引用次数: 0

Recent Development: Enantioselective Extraction in Chiral Separation 手性分离中对映体选择性萃取的研究进展

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-03-03 DOI: 10.26420/AUSTININTERNMED.2021.1130

M. Hashemi, Hadjmohammadi Mr

引用次数: 0

Important of B-Complex Vitamins in Treatment Protocol in Diabetic Patients 复合维生素b在糖尿病患者治疗方案中的重要性

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2021-01-25 DOI: 10.26420/austinjanalpharmchem.2022.1139

Gaafar A, Ayyad R

引用次数: 0

A chiral HPLC-MS/MS method for simultaneous quantification of warfarin enantiomers and its major hydroxylation metabolites of CYP2C9 and CYP3A4 in human plasma. 手性HPLC-MS/MS同时定量人血浆中华法林对映体及其主要羟基化代谢产物CYP2C9和CYP3A4。

Austin journal of analytical and pharmaceutical chemistry Pub Date : 2014-01-01

W Ju, K Peng, S Yang, H Sun, M Sampson, M Z Wang

{"title":"A chiral HPLC-MS/MS method for simultaneous quantification of warfarin enantiomers and its major hydroxylation metabolites of CYP2C9 and CYP3A4 in human plasma.","authors":"W Ju, K Peng, S Yang, H Sun, M Sampson, M Z Wang","doi":"","DOIUrl":"","url":null,"abstract":"Warfarin is an oral anticoagulant that requires frequent therapeutic drug monitoring due to a narrow therapeutic window, considerable interindividual variability in drug response, and susceptibility to drug-drug and drug-diet interactions. Enantiomeric separation and quantification of warfarin enantiomers and clinically important major hydroxylation metabolites are essential for drug interaction studies and phenotypic characterization of CYP2C9 and CYP3A4, the major cytochrome P450 (CYP) enzymes involved in warfarin metabolism. Here, we describe the development and validation of a chiral high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)-based quantification of R-warfarin, S-warfarin, S-7-hydroxywarfarin (the major CYP2C9 metabolite) and (9R;10S)-10-hydroxywarfarin (the CYP3A4 metabolite) in human plasma. Simple protein precipitation-based extraction showed good recovery of analytes (82.9 - 96.9%). The developed method exhibited satisfactory intra-day and inter-day accuracy and precision. The lower limits of detection were 0.25 nM (or ~0.08 ng/mL) for the warfarin enantiomers and 0.1 nM (or ~0.04 ng/mL) for S-7-hydroxywarfarin and (9R;10S)-10-hydroxywarfarin using only 50 µL plasma during extraction. The validated method was successfully applied to analyze plasma samples obtained from a healthy human subject who enrolled in a clinical drug interaction study involving warfarin.","PeriodicalId":91055,"journal":{"name":"Austin journal of analytical and pharmaceutical chemistry","volume":"1 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494745/pdf/nihms693615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33893181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0