RNA & disease (Houston, Tex.)最新文献_第2页

Bivalent aptamer-dual siRNA chimera is emerging as a new combination therapy 二价适体双siRNA嵌合体作为一种新的联合疗法正在出现

RNA & disease (Houston, Tex.) Pub Date : 2017-04-10 DOI: 10.14800/RD.1534

Hong Yan Liu

引用次数: 0

RNA processing and ribosome biogenesis in bone marrow failure dsorders 骨髓衰竭疾病中的RNA加工和核糖体生物发生

RNA & disease (Houston, Tex.) Pub Date : 2017-04-03 DOI: 10.14800/RD.1531

W. Blalock, M. Piazzi, A. Gallo, A. Bavelloni, Enrico Focaccia, I. Faenza

引用次数: 4

A possible link between specific transfer RNA methylation and tumorigenic phenotype of breast cancer 特异性转移RNA甲基化与癌症肿瘤表型之间的可能联系

RNA & disease (Houston, Tex.) Pub Date : 2017-03-27 DOI: 10.14800/RD.1530

K. Tomita

{"title":"A possible link between specific transfer RNA methylation and tumorigenic phenotype of breast cancer","authors":"K. Tomita","doi":"10.14800/RD.1530","DOIUrl":"https://doi.org/10.14800/RD.1530","url":null,"abstract":"The human RNA methyltransferase BCDIN3D is overexpressed in breast cancer cells and involved in cellular invasion and poor prognosis of breast cancer. Several years ago, BCDIN3D was reported to dimethylate the 5'-monophosphate of specific precursor miRNAs (pre-miRNAs), such as the tumor suppressor miR145. Dimethylation of the 5'-monophosphate of the pre-miRNA negatively regulates the subsequent processing by Dicer in vitro , and results in the downregulated expression of the mature form of the miRNA. The depletion of BCDIN3D also reportedly results in the suppression of the tumorigenic phenotype of breast cancer cells. Thus, these findings suggested that BCDIN3D promotes the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs via the dimethylation of the 5'-monophosphate of the corresponding pre-miRNAs . Recently, we found that cytoplasmic tRNA His is actually the primary target of human BCDIN3D, rather than pre-miR145. BCDIN3D monomethylates the 5'-phosphate of cytoplasmic tRNA His much more efficiently than that of pre-miRNA in vitro , and is responsible for the monomethylation of the 5'-phosphate of cytoplasmic tRNA His in vivo . BCDIN3D recognizes the eight-nucleotide long extended acceptor helix with the G -1 -A 73 mis-pair at the top of the acceptor stem of tRNA His , which are exceptional features among cytoplasmic tRNA species. These results not only reveal the primary target of BCDIN3D, which is overexpressed in breast cancer cells, but also highlight the possible involvement of the 5'-phosphomethylation of tRNA and/or tRNA in the tumorigenesis of breast cancer cells, beyond its established function in protein synthesis.","PeriodicalId":90965,"journal":{"name":"RNA & disease (Houston, Tex.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41390687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A novel microRNA-1207-3p/FNDC1/FN1/AR regulatory pathway in prostate cancer. 一种新的微小RNA-1207-3p/FNDC1/FN1/AR调节途径在前列腺癌症中的应用。

RNA & disease (Houston, Tex.) Pub Date : 2017-02-06 DOI: 10.14800/RD.1503

D. Das, O. Ogunwobi

{"title":"A novel microRNA-1207-3p/FNDC1/FN1/AR regulatory pathway in prostate cancer.","authors":"D. Das, O. Ogunwobi","doi":"10.14800/RD.1503","DOIUrl":"https://doi.org/10.14800/RD.1503","url":null,"abstract":"Prostate cancer (PCa) is the second most common cause of cancer-specific deaths in the U.S. Unfortunately, the underlying molecular mechanisms for its development and progression remain unclear. Studies have established that microRNAs (miRNAs) are dysregulated in PCa. The intron-derived microRNA-1207-3p (miR-1207-3p) is encoded at the non-protein coding gene locus PVT1 on the 8q24 human chromosomal region, an established PCa susceptibility locus. However, miR-1207-3p in PCa had not previously been investigated. Therefore, we explored if miR-1207-3p plays any regulatory role in PCa. We discovered that miR-1207-3p is significantly underexpressed in PCa cell lines in comparison to normal prostate epithelial cells, and that increased expression of microRNA-1207-3p in PCa cells significantly inhibits proliferation, migration, and induces apoptosis via direct molecular targeting of fibronectin type III domain containing 1 (FNDC1). Our studies also revealed significant overexpression of FNDC1, fibronectin (FN1) and the androgen receptor (AR) in human PCa cell lines as well as tissues, and FNDC1, FN1, and AR positively correlate with aggressive PCa. These findings, recently published in Experimental Cell Research, are the first to describe a novel miR-1207-3p/FNDC1/FN1/AR novel regulatory pathway in PCa.","PeriodicalId":90965,"journal":{"name":"RNA & disease (Houston, Tex.)","volume":"4 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46487406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Technologies of high-throughput tissue-specific gene expression profiling 高通量组织特异性基因表达谱技术

RNA & disease (Houston, Tex.) Pub Date : 2017-01-24 DOI: 10.14800/RD.1492

Zhichao Xu, Xiao Liu

引用次数: 0

A novel microRNA-1207-3p/FNDC1/FN1/AR regulatory pathway in prostate cancer. 前列腺癌中一个新的microRNA-1207-3p/FNDC1/FN1/AR调控通路

RNA & disease (Houston, Tex.) Pub Date : 2017-01-01 Epub Date: 2017-02-06

Dibash K Das, Olorunseun O Ogunwobi

{"title":"A novel microRNA-1207-3p/FNDC1/FN1/AR regulatory pathway in prostate cancer.","authors":"Dibash K Das, Olorunseun O Ogunwobi","doi":"","DOIUrl":"","url":null,"abstract":"Prostate cancer (PCa) is the second most common cause of cancer-specific deaths in the U.S. Unfortunately, the underlying molecular mechanisms for its development and progression remain unclear. Studies have established that microRNAs (miRNAs) are dysregulated in PCa. The intron-derived microRNA-1207-3p (miR-1207-3p) is encoded at the non-protein coding gene locus PVT1 on the 8q24 human chromosomal region, an established PCa susceptibility locus. However, miR-1207-3p in PCa had not previously been investigated. Therefore, we explored if miR-1207-3p plays any regulatory role in PCa. We discovered that miR-1207-3p is significantly underexpressed in PCa cell lines in comparison to normal prostate epithelial cells, and that increased expression of microRNA-1207-3p in PCa cells significantly inhibits proliferation, migration, and induces apoptosis via direct molecular targeting of fibronectin type III domain containing 1 (FNDC1). Our studies also revealed significant overexpression of FNDC1, fibronectin (FN1) and the androgen receptor (AR) in human PCa cell lines as well as tissues, and FNDC1, FN1, and AR positively correlate with aggressive PCa. These findings, recently published in Experimental Cell Research, are the first to describe a novel miR-1207-3p/FNDC1/FN1/AR novel regulatory pathway in PCa.","PeriodicalId":90965,"journal":{"name":"RNA & disease (Houston, Tex.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328418/pdf/nihms850185.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34776054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

microRNA-29 mediates a novel negative feedback loop to regulate SCAP/SREBP-1 and lipid metabolism. microRNA-29介导一个新的负反馈回路来调节SCAP/SREBP-1和脂质代谢。

RNA & disease (Houston, Tex.) Pub Date : 2017-01-01 Epub Date: 2017-03-20 DOI: 10.14800/rd.1525

Peng Ru, Deliang Guo

{"title":"microRNA-29 mediates a novel negative feedback loop to regulate SCAP/SREBP-1 and lipid metabolism.","authors":"Peng Ru, Deliang Guo","doi":"10.14800/rd.1525","DOIUrl":"https://doi.org/10.14800/rd.1525","url":null,"abstract":"The membrane-bound transcription factors, SREBPs (sterol regulatory element-binding proteins), play a central role in regulating lipid metabolism. The transcriptional activation of SREBPs requires the key protein SCAP (SREBP-cleavage activating protein) to translocate their precursors from the endoplasmic reticulum to the Golgi for subsequent proteolytic activation, a process tightly regulated by a cholesterol-mediated negative feedback loop. Our previous work showed that the SCAP/SREBP-1 pathway is significantly upregulated in human glioblastoma (GBM), the most deadly brain cancer, and that glucose-mediated N-glycosylation of SCAP is a prerequisite step for SCAP/SREBP trafficking. More recently, we demonstrated that microRNA-29 (miR-29) mediates a previously unrecognized negative feedback loop in SCAP/SREBP-1 signaling to control lipid metabolism. We found that SREBP-1, functioning as a transcription factor, promotes the expression of the miR-29 family members, miR-29a, -29b and -29c. In turn, the miR-29 isoforms reversely repress the expression of SCAP and SREBP-1. Moreover, treatment with miR-29 mimics effectively suppressed GBM tumor growth by inhibiting SCAP/SREBP-1 and de novo lipid synthesis. These findings, recently published in Cell Reports, strongly suggest that delivery of miR-29 in vivo may be a promising approach to treat cancer and metabolic diseases by suppressing SCAP/SREBP-1-regulated lipid metabolism.","PeriodicalId":90965,"journal":{"name":"RNA & disease (Houston, Tex.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485916/pdf/nihms861933.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35131129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Triggerable RNA nanodevices. 可触发的 RNA 纳米器件。

RNA & disease (Houston, Tex.) Pub Date : 2017-01-01 Epub Date: 2017-06-21

Justin Halman, Emily Satterwhite, Jaclyn Smollett, Eckart Bindewald, Lorena Parlea, Mathias Viard, Paul Zakrevsky, Wojciech K Kasprzak, Kirill A Afonin, Bruce A Shapiro

引用次数: 0

One aptamer, two functions: the full-length aptamer inhibits AMPA receptors, while the short one inhibits both AMPA and kainate receptors. 一个适体，两种功能:全长适体抑制AMPA受体，而短的适体同时抑制AMPA和盐酸盐受体。

RNA & disease (Houston, Tex.) Pub Date : 2017-01-01 Epub Date: 2017-06-12

William J Jaremko, Zhen Huang, Wei Wen, Andrew Wu, Nicholas Karl, Li Niu

{"title":"One aptamer, two functions: the full-length aptamer inhibits AMPA receptors, while the short one inhibits both AMPA and kainate receptors.","authors":"William J Jaremko, Zhen Huang, Wei Wen, Andrew Wu, Nicholas Karl, Li Niu","doi":"","DOIUrl":"","url":null,"abstract":"AMPA and kainate receptors, along with NMDA receptors, are distinct subtypes of glutamate ion channels. Excessive activity of AMPA and kainate receptors has been implicated in neurological diseases, such as epilepsy and neuropathic pain. Antagonists that block their activities are therefore potential drug candidates. In a recent article in the Journal of Biological Chemistry by Jaremko et al. 2017, we have reported on the discovery and molecular characterization of an RNA aptamer of a dual functionality: the full-length RNA (101 nucleotide) inhibits AMPA receptors while the truncated or the short (55 nucleotide) RNA inhibits both the AMPA and kainate receptors. The full-length RNA aptamer was isolated through a specially designed, systematic evolution of ligands by exponential enrichment (SELEX) using only a single type of AMPA receptors expressed in HEK-293 cells. The design feature and the results of our recent article are highlighted here, as they demonstrate the utility of the SELEX approach and the potential of using a single AMPA receptor type to develop potent, novel RNA aptamers targeting multiple subunits and AMPA/kainate receptor subtypes with length-dependent functionalities.","PeriodicalId":90965,"journal":{"name":"RNA & disease (Houston, Tex.)","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35320042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The miR-125a and miR-320c are potential tumor suppressor microRNAs epigenetically silenced by the polycomb repressive complex 2 in multiple myeloma. 在多发性骨髓瘤中，miR-125a和miR-320c是潜在的肿瘤抑制microrna，在表观遗传上被多梳抑制复合体2沉默。

RNA & disease (Houston, Tex.) Pub Date : 2017-01-01 Epub Date: 2017-04-03 DOI: 10.14800/rd.1529

Mohammad Alzrigat, Helena Jernberg-Wiklund

{"title":"The miR-125a and miR-320c are potential tumor suppressor microRNAs epigenetically silenced by the polycomb repressive complex 2 in multiple myeloma.","authors":"Mohammad Alzrigat, Helena Jernberg-Wiklund","doi":"10.14800/rd.1529","DOIUrl":"https://doi.org/10.14800/rd.1529","url":null,"abstract":"We have previously presented the histone methyltransferase enhancer of zeste homolog 2 (EZH2) of the polycomb repressive complex 2 (PRC2) as a potential therapeutic target in Multiple Myeloma (MM). In a recent article in Oncotarget by Alzrigat. et al. 2017, we have reported on the novel finding that EZH2 inhibition using the highly selective inhibitor of EZH2 enzymatic activity, UNC1999, reactivated the expression of microRNA genes previously reported to be underexpressed in MM. Among these, we have identified miR-125a-3p and miR-320c as potential tumor suppressor microRNAs as they were predicted to target MM-associated oncogenes; IRF-4, XBP-1 and BLIMP-1. We also found EZH2 inhibition to reactivate the expression of miR-494, a previously reported regulator of the c-MYC oncogene. In addition, we could report that EZH2 inhibition downregulated the expression of a few well described oncogenic microRNAs in MM. The data from our recent article are here highlighted as it shed a new light onto the oncogenic function of the PRC2 in MM. These data further strengthen the notion that the PRC2 complex may be of potential therapeutic interest.","PeriodicalId":90965,"journal":{"name":"RNA & disease (Houston, Tex.)","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35131130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23