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Hispanic and Catholic, or Hispanic-Catholic? Racialized Religious Identity for Self-Identified “Hispanic” Students at a Predominantly White Institution 西班牙裔天主教徒，还是西班牙裔天主教徒?在一所以白人为主的大学里，自我认定的“西班牙裔”学生的种族化宗教身份
Next generation, sequencing & applications Pub Date : 2019-01-01 DOI: 10.33011/next/5/1/1
C. Dalessandro
{"title":"Hispanic and Catholic, or Hispanic-Catholic? Racialized Religious Identity for Self-Identified “Hispanic” Students at a Predominantly White Institution","authors":"C. Dalessandro","doi":"10.33011/next/5/1/1","DOIUrl":"https://doi.org/10.33011/next/5/1/1","url":null,"abstract":"Drawing from in-depth interviews with undergraduates at a predominantly non-Hispanic white and secular university in the U.S., I investigate how those self-identified as both Hispanic and Catholic negotiate the identity statuses that classify them as minorities on their campus. While participants in this study who did not identify as Hispanic also did not make connections between religious affiliation and racial/ethnic group membership, most Hispanic undergraduates did make these connections. I found that self-identified Hispanic students used the meanings they attach to Catholic religious affiliation to help them also make sense of their racialized Hispanic identities in the university space. Despite coming from diverse geographic, social class, and cultural backgrounds, these students move toward creating a shared definition of what it means to be a racialized Hispanic person (or Latinx person with Spanish-speaking ancestors) in this particular collegiate context. I conclude with suggestions for future research.","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":" 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72382460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Apostle Paul in Spinoza and Badiou 斯宾诺莎和巴迪欧的使徒保罗
Next generation, sequencing & applications Pub Date : 2019-01-01 DOI: 10.33011/next/5/1/2
P. A. Rodríguez
{"title":"The Apostle Paul in Spinoza and Badiou","authors":"P. A. Rodríguez","doi":"10.33011/next/5/1/2","DOIUrl":"https://doi.org/10.33011/next/5/1/2","url":null,"abstract":"This paper offers an analysis of the philosophical interpretations of the apostle Paul in Spinoza’s Tractatus Theologico-Politicus (1669) and Badiou's Saint Paul: La foundation de l'universalisme (1997). In addition, a brief description is offered on the difference between, on the one hand, the theological Paul, the Apostle of Faith, and, on the other hand, the historical Paul. Both Spinoza and Badiou offer complex philosophical readings of Paul that can be better understood when taking into consideration how they square with the image of Paul that has been constructed by the various theological traditions of Europe (i.e., the theological Paul), as well as the figure of Paul that is currently being reconstructed by historical-critical biblical scholarship (both New Perspective on Paul and postcolonial/empire-critical readings of Paul).","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83501303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Violence of Nonviolence: Contextualizing the Movements of King and Gandhi 非暴力的暴力:金和甘地运动的背景
Next generation, sequencing & applications Pub Date : 2019-01-01 DOI: 10.33011/next/5/1/4
Greg Mileski
{"title":"The Violence of Nonviolence: Contextualizing the Movements of King and Gandhi","authors":"Greg Mileski","doi":"10.33011/next/5/1/4","DOIUrl":"https://doi.org/10.33011/next/5/1/4","url":null,"abstract":"King and Gandhi: two names that have come to be synonymous with nonviolence. And yet, the movements they led responded to and, in some cases with, significant violence. In a recent paper (2016), August H. Nimtz analyzes the role of violence in the movement of Dr. King, concluding that violence played a significant role in the success of the African-American Civil Rights Movement. Using Nimtz’s work as a starting point, this paper analyzes King’s movement and views, comparing definitions of “principled nonviolence” versus “pragmatic nonviolence.” From there, this paper analyzes the role of violence in the struggle for Indian independence from British colonialism and Gandhi’s own views on when, if ever, violence is appropriate. This paper concludes that, indeed, violence—that of sanctioned, state-sponsored violence and that of non-sanctioned actors—has had significant roles in both of these movements. In what way, then, could these movements be said to be nonviolent? Finally, this paper asks why there remains such an impetus to identify these movements, and their leaders, with “principled nonviolence.”","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82225046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Genomics of Colorectal Cancer in African Americans. 非裔美国人结直肠癌基因组学研究。
Next generation, sequencing & applications Pub Date : 2016-09-21 DOI: 10.4172/2469-9853.1000133
H. Brim, H. Ashktorab
{"title":"Genomics of Colorectal Cancer in African Americans.","authors":"H. Brim, H. Ashktorab","doi":"10.4172/2469-9853.1000133","DOIUrl":"https://doi.org/10.4172/2469-9853.1000133","url":null,"abstract":"Genome-wide studies are increasingly becoming a must, especially for complex diseases such as cancer where multiple genes and diverse molecular mechanisms are known to be involved in genes' function alteration. In this review, we report our latest genomic and epigenomic findings in African-American colorectal cancer patients. This population suffers a higher burden of the disease and most investigators in this field are looking for the underlying genetic and epigenetic targets that might be responsible for this disparity. We here report genome-wide copy number variations, single nucleotide mutations and DNA methylation findings that might be specific to this population.","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82112431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Targeted Exome Sequencing Outcome Variations of Colorectal Tumors within and across Two Sequencing Platforms. 结直肠肿瘤的靶向外显子组测序结果在两个测序平台内和之间的差异。
Next generation, sequencing & applications Pub Date : 2016-06-01 Epub Date: 2016-03-14 DOI: 10.4172/2469-9853.1000123
Hassan Ashktorab, Hamed Azimi, Michael L Nickerson, Sara Bass, Sudhir Varma, Hassan Brim
{"title":"Targeted Exome Sequencing Outcome Variations of Colorectal Tumors within and across Two Sequencing Platforms.","authors":"Hassan Ashktorab,&nbsp;Hamed Azimi,&nbsp;Michael L Nickerson,&nbsp;Sara Bass,&nbsp;Sudhir Varma,&nbsp;Hassan Brim","doi":"10.4172/2469-9853.1000123","DOIUrl":"https://doi.org/10.4172/2469-9853.1000123","url":null,"abstract":"<p><strong>Background and aim: </strong>Next generation sequencing (NGS) has quickly the tool of choice for genome and exome data generation. The multitude of sequencing platforms as well as the variabilities within each platform need to be assessed. In this paper we used two platforms (ION TORRENT AND ILLUMINA) to assess single nucleotides variants in colorectal cancer (CRC) specimens.</p><p><strong>Methods: </strong>CRC specimens (n = 13) collected from 6 CRC (cancer and matched normal) patients were used to establish the mutational profile using ION TORRENT AND ILLUMINA sequencing platforms. We analyzed a set of samples from Formalin Fixed Paraffin Embedded and FF (FF) samples on both platforms to assess the effect of sample nature (FFPE vs. FF) on sequencing outcome and to evaluate the similarity/differences of SNVs across the two platforms. In addition, duplicates of FF samples were sequenced on each platform to assess variability within platform.</p><p><strong>Results: </strong>The comparison of FF replicates to each other gave a concordance of 77% (± 15.3%) in Ion Torrent and 70% (± 3.7%) in Illumina. FFPE vs. FF replicates gave a concordance of 40% (± 32%) in Ion Torrent and 49% (± 19%) in Illumina. For the cross platform concordance were FFPE compared to FF (Average of 75% (± 9.8%) for FFPE samples and 67% (± 32%) for FF and 70% (± 26.8%) overall average).</p><p><strong>Conclusion: </strong>Our data show a significant variability within and across platforms. Also the number of detected variants depend on the nature of the specimen; FF vs. FFPE. Validation of NGS discovered mutations is a must to rule-out false positive mutants. This validation might either be performed through a second NGS platform or through Sanger sequencing.</p>","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2469-9853.1000123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34382775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Accuracy of Next Generation Sequencing Platforms. 下一代测序平台的准确性。
Next generation, sequencing & applications Pub Date : 2014-01-01 DOI: 10.4172/jngsa.1000106
Edward J Fox, Kate S Reid-Bayliss, Mary J Emond, Lawrence A Loeb
{"title":"Accuracy of Next Generation Sequencing Platforms.","authors":"Edward J Fox,&nbsp;Kate S Reid-Bayliss,&nbsp;Mary J Emond,&nbsp;Lawrence A Loeb","doi":"10.4172/jngsa.1000106","DOIUrl":"https://doi.org/10.4172/jngsa.1000106","url":null,"abstract":"<p><p>Next-generation DNA sequencing has revolutionized genomic studies and is driving the implementation of precision diagnostics. The ability of these technologies to disentangle sequence heterogeneity, however, is limited by their relatively high error rates. A Several single molecule barcoding strategies have been propose to reduce the overall error frequency. A Duplex Sequencing additionally exploits the fact that DNA is double-strand, with one strand reciprocally encoding the sequence information of its complement, and can eliminate nearly all sequencing errors by comparing the sequence of individually tagged amplicons derived from one strand of DNA with that of its complementary strand. This method reduces errors to fewer than one per ten million nucleotides sequenced.</p>","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"1 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/jngsa.1000106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33068919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 221
Molecular Cloning, Modeling, and Characterization of Type 2 Metallothionein from Plantago ovata Forsk 车前草2型金属硫蛋白的分子克隆、建模及表征
Next generation, sequencing & applications Pub Date : 2013-03-13 DOI: 10.1155/2013/756983
Amitava Moulick, Debashis Mukhopadhyay, Shonima Talapatra, Nirmalya Ghoshal, S. Raychaudhuri
{"title":"Molecular Cloning, Modeling, and Characterization of Type 2 Metallothionein from Plantago ovata Forsk","authors":"Amitava Moulick, Debashis Mukhopadhyay, Shonima Talapatra, Nirmalya Ghoshal, S. Raychaudhuri","doi":"10.1155/2013/756983","DOIUrl":"https://doi.org/10.1155/2013/756983","url":null,"abstract":"Plantago ovata Forsk is a medicinally important plant. Metallothioneins are cysteine rich proteins involved in the detoxification of heavy metals. Molecular cloning and modeling of MT from P. ovata is not reported yet. The present investigation will describe the isolation, structure prediction, characterization, and expression under copper stress of type 2 metallothionein (MT2) from this species. The gene of the protein comprises three exons and two introns. The deduced protein sequence contains 81 amino acids with a calculated molecular weight of about 8.1 kDa and a theoretical pI value of 4.77. The transcript level of this protein was increased in response to copper stress. Homology modeling was used to construct a three-dimensional structure of P. ovata MT2. The 3D structure model of P. ovata MT2 will provide a significant clue for further structural and functional study of this protein.","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"42 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2013-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74274285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Identification of Prophages and Prophage Remnants within the Genome of Avibacterium paragallinarum Bacterium 副allinarum Avibacterium细菌基因组中原噬菌体和残体的鉴定
Next generation, sequencing & applications Pub Date : 2012-12-18 DOI: 10.1155/2012/953609
Y. Roodt, R. Bragg, J. Albertyn
{"title":"Identification of Prophages and Prophage Remnants within the Genome of Avibacterium paragallinarum Bacterium","authors":"Y. Roodt, R. Bragg, J. Albertyn","doi":"10.1155/2012/953609","DOIUrl":"https://doi.org/10.1155/2012/953609","url":null,"abstract":"Bacterial whole genome sequencing has delivered an abundance of prophage sequences as a by-product and the analysis of these sequences revealed ways in which phages have affected the genome of their host bacteria in various bacterial species. The aim of this study was to identify the phage-related sequences in the draft assembly of the Avibacterium paragallinarum genome, the causative agent of infectious coryza in poultry. Whole genome assembly was not possible due to the presence of gaps and/or repeats existent on the ends of contigs. However, genome annotation revealed prophage and prophage remnant sequences present in this genome. From the results obtained, a complete Mu-like bacteriophage could be identified that was termed AvpmuC-2M. A complete sequence of HP2-like bacteriophage, named AvpC-2M-HP2, was also identified.","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"23 2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2012-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78025410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Cost-Effective Paramagnetic Bead Technique for Purification of Cycle Sequencing Products 高性价比的顺磁珠循环测序产品纯化技术
Next generation, sequencing & applications Pub Date : 2012-09-23 DOI: 10.1155/2012/767959
S. Mijatovic-Rustempasic, M. Frace, M. Bowen
{"title":"Cost-Effective Paramagnetic Bead Technique for Purification of Cycle Sequencing Products","authors":"S. Mijatovic-Rustempasic, M. Frace, M. Bowen","doi":"10.1155/2012/767959","DOIUrl":"https://doi.org/10.1155/2012/767959","url":null,"abstract":"The quality of sequencing results depends greatly upon the quality and purity of the template as well as the purity of the fluorescently labeled products generated by cycle sequencing. Numerous approaches have been used for purification of cycle sequencing products, including alcohol precipitation, affinity-based chromatography, size exclusion chromatography, commercially-available proprietary methods, and paramagnetic bead technology. In this paper, we describe an affordable paramagnetic technology method using BioMag Carboxyl beads. Compared to other well-established, proprietary methods for purification of cycle sequencing products, this method produced consistently good results, with a very low reagent cost and short procedure time.","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"86 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2012-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80680238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer 高香兰杂种Vanda Mimi Palmer乙酰辅酶a - c -乙酰转移酶cDNA的克隆、测序和鉴定
Next generation, sequencing & applications Pub Date : 2012-06-19 DOI: 10.1155/2012/509034
Seow-Ling Teh, J. Abdullah, P. Namasivayam
{"title":"Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer","authors":"Seow-Ling Teh, J. Abdullah, P. Namasivayam","doi":"10.1155/2012/509034","DOIUrl":"https://doi.org/10.1155/2012/509034","url":null,"abstract":"Vanda Mimi Palmer, a hybrid of Vanda Tan Chay Yan and Vanda tessellata (Roxb.) Hk.f. ex G. Don, is cultivated as a potted ornamental plant mainly for its fragrance rather than its look. Plant acetyl-CoA-C-acetyltransferase (ACA) is involved in the condensation of two acetyl-CoAs to form acetoacetyl-CoA, which condenses with another acetyl-CoA to yield a crucial molecule, 3-hydroxyl-3-methylglutaryl-CoA, at the initial step of the mevalonate (MVA) pathway. An ACA gene from vandaceous orchid has never been reported. We describe the isolation and molecular characterization of an ACA-like gene from V. Mimi Palmer (designated as VMPACA) to facilitate a better understanding of the terpenoid biosynthesis pathway in orchids. The deduced VMPACA encodes a 376-amino-acid protein with a molecular weight of 39 kDa, which comprises an open reading frame of 1128 bp. It is flanked by 87 bp of 5′-untranslated region and 174 bp of 3′-untranslated region including a poly-A tail. Its protein sequence is 81% identical to other plant ACAs and contains a thiolase active site. The fluctuation expression pattern of VMPACA transcript by real-time RT-PCR showed that it is developmentally and temporally regulated with predominant expression in outer and lateral inner tepals compared to vegetative tissues.","PeriodicalId":90934,"journal":{"name":"Next generation, sequencing & applications","volume":"144 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2012-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79638511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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