Biology of the neonate最新文献

Frequencies of A(TA)7TAA, G71R, and G493R mutations of the UGT1A1 gene in the Malaysian population. 马来西亚人群UGT1A1基因A(TA)7TAA、G71R和G493R突变的频率

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2005-10-06 DOI: 10.1159/000088844

Surini Yusoff, Hans Van Rostenberghe, Narazah M Yusoff, Norlelawati A Talib, Noraida Ramli, N Zainal A N Ismail, W Pauzi W Ismail, Masafumi Matsuo, Hisahide Nishio

{"title":"Frequencies of A(TA)7TAA, G71R, and G493R mutations of the UGT1A1 gene in the Malaysian population.","authors":"Surini Yusoff, Hans Van Rostenberghe, Narazah M Yusoff, Norlelawati A Talib, Noraida Ramli, N Zainal A N Ismail, W Pauzi W Ismail, Masafumi Matsuo, Hisahide Nishio","doi":"10.1159/000088844","DOIUrl":"https://doi.org/10.1159/000088844","url":null,"abstract":"Background: Gilbert syndrome is caused by defects in the uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) gene. These mutations differ among different populations and many of them have been found to be genetic risk factors for the development of neonatal jaundice.Objectives: The objective was to determine the frequencies of the following mutations in the UGT1A1 gene: A(TA)7TAA (the most common cause of Gilbert syndrome in Caucasians), G71R (more common in the Japanese and Taiwanese population), and G493R (described in a homozygous Malay woman with Crigler-Najjar syndrome type 2) in a group of Malaysian babies with hyperbilirubinemia and a group of normal controls.Methods: The GeneScan fragment analysis was used to detect the A(TA)7TAA variant. Mutation screening of both G71R and G493R was performed using denaturing high performance liquid chromatography.Results: Fourteen out of fifty-five neonates with hyperbilirubinemia (25%) carried the A(TA)7TAA mutation (10 heterozygous, 4 homozygous). Seven out of fifty controls (14%) carried this mutation (6 heterozygous, 1 homozygous). The allelic frequencies for hyperbilirubinemia and control patients were 16 and 8%, respectively (p=0.20). Heterozygosity for the G71R mutation was almost equal among both groups (5.5% for hyperbilirubinemia patients and 6.0% for controls; p=0.61). One subject (1.8%) in the hyperbilirubinemia group and none of the controls were heterozygous for the G493R mutation (p=0.476).Conclusions: The A(TA)7TAA seems more common than the G71R and G493R mutations in the Malaysian population.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"89 3","pages":"171-6"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000088844","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25644775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Relaxant effects of the soluble guanylate cyclase activator and NO sensitizer YC-1 in piglet pulmonary arteries. 可溶性鸟苷酸环化酶激活剂和NO增敏剂YC-1对仔猪肺动脉的松弛作用。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-03-09 DOI: 10.1159/000091968

Gema González-Luis, Angel Cogolludo, Laura Moreno, Federica Lodi, Juan Tamargo, Francisco Pérez-Vizcaíno, Eduardo Villamor

{"title":"Relaxant effects of the soluble guanylate cyclase activator and NO sensitizer YC-1 in piglet pulmonary arteries.","authors":"Gema González-Luis, Angel Cogolludo, Laura Moreno, Federica Lodi, Juan Tamargo, Francisco Pérez-Vizcaíno, Eduardo Villamor","doi":"10.1159/000091968","DOIUrl":"https://doi.org/10.1159/000091968","url":null,"abstract":"Background: The indazole derivative YC-1 has been characterized as a nitric oxide (NO)-independent and heme dependent soluble guanylate cyclase (sGC) activator, which also sensitizes sGC to NO.Objective: To examine the effects of YC-1 on vascular relaxation in newborn and 2-week-old piglet pulmonary arteries. The effect of YC-1 on the relaxation induced by exogenous NO was also analyzed.Methods: Isolated rings from third branch pulmonary arteries and fifth-seventh-generation intrapulmonary arterioles were mounted in organ chambers for isometric tension recording. Arteries were precontracted with the thromboxane A2 mimetic U46619.Results: YC-1 induced relaxation was greater in 2-week-old pulmonary arteries and was abolished by the sGC inhibitor ODQ (10 microM). YC-1 induced relaxation was similar in conduit pulmonary arteries and arterioles. In the 2-week-old conduit pulmonary arteries, the response to YC-1 was significantly reduced when the endothelium was removed or after incubation with the NO synthase inhibitor L-NAME (0.1 mM). YC-1 augmented NO-induced relaxation in 2-week-old but not in neonatal conduit pulmonary arteries.Conclusions: Our results indicate that YC-1 induced pulmonary vascular relaxation in conduit and resistance pulmonary arteries and these effects increased with postnatal age. In the 2-week-old conduit pulmonary arteries and besides being a direct activator of sGC, YC-1 produced endothelium-dependent relaxation and synergized with exogenous NO.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"90 1","pages":"66-72"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000091968","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25904220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Fetal macrosomia--a continuing obstetric challenge. 胎儿巨大儿——一个持续的产科挑战。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-03-16 DOI: 10.1159/000092042

Nonna Heiskanen, Kaisa Raatikainen, Seppo Heinonen

{"title":"Fetal macrosomia--a continuing obstetric challenge.","authors":"Nonna Heiskanen, Kaisa Raatikainen, Seppo Heinonen","doi":"10.1159/000092042","DOIUrl":"https://doi.org/10.1159/000092042","url":null,"abstract":"Background: Macrosomic fetuses represent a continuing challenge in obstetrics.Objectives: We studied maternal risk factors of fetal macrosomia and maternal and infant outcome in such cases.Methods: A retrospective cohort study was carried out with a total of 26,961 singleton pregnancies between 1989 and 2001. Records of 886 mothers who gave birth to live born infants weighing > or =4,500 g were compared to those of 26,075 mothers with normal weight (<4,500 g) infants. Multiple regression analysis was used to identify independent reproductive risk factors. Perinatal complications were also assessed.Results: The incidence of fetal macrosomia was 3.4%. Diabetes, previous macrosomic birth, postdatism (>42 weeks of gestation), obesity (BMI > 25 before pregnancy), male infant, gestational diabetes mellitus, and non-smoking were independent risk factors of fetal macrosomia, with adjusted risks of 4.6, 3.1, 3.1, 2.0, 1.9, 1.6, 1.4, respectively. In the macrosomic group, birth and maternal traumas occurred significantly more often than in the control group. However, records of subsequent pregnancies (n = 250) after the study period showed that a previous uncomplicated birth appeared to decrease complication risks.Conclusions: Most cases of fetal macrosomia occur in low-risk pregnancies and evaluation of maternal risks cannot accurately predict which women will eventually give birth to an overweight newborn. After an uncomplicated birth of a macrosomic infant, vaginal delivery may be a safe option for the infant and mother.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"90 2","pages":"98-103"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000092042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25915585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 90

Systemic and pulmonary effects of vasopressors and inotropes in the neonate. 血管加压剂和收缩性药物对新生儿全身和肺部的影响。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-06-01 DOI: 10.1159/000092872

Istvan Seri

引用次数: 6

Acceleration of lung maturation in a human fetus following maternal isotretinoin intake. 母体异维甲酸摄入后人类胎儿肺成熟的加速。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-05-29 DOI: 10.1159/000093589

Jean-Claude Fauchère, Jorg Ersch, Daniel Allen Grant, Roland Zimmermann, Hans Ulrich Bucher, Thomas Stallmach

{"title":"Acceleration of lung maturation in a human fetus following maternal isotretinoin intake.","authors":"Jean-Claude Fauchère, Jorg Ersch, Daniel Allen Grant, Roland Zimmermann, Hans Ulrich Bucher, Thomas Stallmach","doi":"10.1159/000093589","DOIUrl":"https://doi.org/10.1159/000093589","url":null,"abstract":"The viability of the human fetus increases significantly beyond 25 weeks' gestation as the lung development progresses towards the 'saccular' stage. We report on a fetus of 22 weeks' gestation whose lung maturation was accelerated by 4 weeks, most likely due to the unintentional exposure to the retinoid isotretinoin (13-cis-retinoic acid) during pregnancy. Although retinoids are known to be stored within the lungs and to play a key role in lung differentiation and growth, their storage within the lung is limited during this critical developmental period. Even though glucocorticosteroids are used clinically to enhance lung maturation in the face of impending preterm birth, there are no data yet which demonstrate that glucocorticosteroids, when given alone, are effective in promoting lung maturation prior to 24 weeks' gestation. Strong evidence however, indicates that glucocorticosteroids promote the utilization of lung retinoids immediately before birth. Our observation of increased lung maturation, in conjunction with the above information suggests that retinoids alone or in combination with glucocorticosteroids might promote lung maturation more effectively than glucocorticosteroids alone when birth seems inevitable at a very early gestational age.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"90 3","pages":"203-6"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000093589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26051379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Transgenic glucocorticoid receptor expression driven by the SP-C promoter reduces neonatal lung cellularity and midkine expression in GRhypo mice. 由SP-C启动子驱动的转基因糖皮质激素受体表达降低了GRhypo小鼠新生肺细胞和midkine的表达。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-03-03 DOI: 10.1159/000091844

Stéphane Gagnon, Wayhuni Atmodjo, Daryl Humes, Colin McKerlie, Feige Kaplan, Neil B Sweezey

{"title":"Transgenic glucocorticoid receptor expression driven by the SP-C promoter reduces neonatal lung cellularity and midkine expression in GRhypo mice.","authors":"Stéphane Gagnon, Wayhuni Atmodjo, Daryl Humes, Colin McKerlie, Feige Kaplan, Neil B Sweezey","doi":"10.1159/000091844","DOIUrl":"https://doi.org/10.1159/000091844","url":null,"abstract":"Background: Congenital truncation of the glucocorticoid receptor (GR) is known to lead to lethal lung immaturity in newborn mice associated with increased lung cellularity (ratio of tissue to airspace) and, as we previously showed, prolonged expression of the retinoid-responsive growth factor midkine.Objectives: We sought to determine if these changes would be reversed by transgenic expression of GR exclusively in the distal airway epithelium.Methods: Mice were generated with expression of transgenic rat (r) GR driven by the human (h) SP-C promoter, on a background of congenital GR truncation.Results: Transgenic epithelial GR expression reduced lung cellularity and midkine expression to levels comparable to wild-type littermates. Nevertheless, the newborn transgenic mice still displayed respiratory failure. Moreover, epithelial expression of the GR transgene did not alter expression of a number of important markers of lung maturation.Conclusions: Our data demonstrating normalization of the lung tissue to airspace ratio in neonatal mice expressing transgenic GR in the distal airway epithelium is consistent with the concept that normal mesenchymal cell loss is due to GR-responsive stimulation from epithelial cells. However, we could find no evidence of altered apoptotic activity between the groups of mice. We speculate that correction of the severe neonatal lung phenotype of GR-deficient mice will require expression of normal GR in non-epithelial as well as epithelial tissues.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"90 1","pages":"46-57"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000091844","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25902071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Rac2 as a potential cause of impaired neonatal neutrophil chemotaxis. Commentary to Meade et al.: Rac2 concentrations in umbilical cord neutrophils (biol neonate 2006;90:156-159). Rac2是新生儿中性粒细胞趋化性受损的潜在原因。对 Meade 等人的评论脐带中性粒细胞中的 Rac2 浓度》（biol neonate 2006;90:156-159）。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-03-30 DOI: 10.1159/000092452

Henry J Rozycki

引用次数: 0

Outcome of extremely preterm infants randomized at birth to different PaCO2 targets during the first seven days of life. 出生时随机分配到出生后7天内不同PaCO2目标的极早产儿的结局

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-04-19 DOI: 10.1159/000092723

Ulrich H Thome, William Carroll, Tzong-Jin Wu, Robert B Johnson, Claire Roane, Daniel Young, Waldemar A Carlo

{"title":"Outcome of extremely preterm infants randomized at birth to different PaCO2 targets during the first seven days of life.","authors":"Ulrich H Thome, William Carroll, Tzong-Jin Wu, Robert B Johnson, Claire Roane, Daniel Young, Waldemar A Carlo","doi":"10.1159/000092723","DOIUrl":"https://doi.org/10.1159/000092723","url":null,"abstract":"Background: Ventilation with higher PaCO(2) goals may reduce lung injury and bronchopulmonary dysplasia (BPD). The effect may be enhanced by using a higher PaCO(2) goal than in previous trials.Objective: To determine the clinical benefits and safety of higher PaCO(2) goals for ventilated preterm infants.Study design: Preterm infants with a gestational age between 23 and 28 completed weeks receiving mechanical ventilation within 6 h of birth were randomized to be managed with either a PaCO(2) target between 55 and 65 mm Hg (7.3- 8.7 kPa, minimal ventilation) or 35 and 45 mm Hg (4.7- 6.0 kPa, routine ventilation) for the first 7 days of life. The primary outcome measure was BPD, defined as need for mechanical ventilation or supplemental oxygen at 36 weeks postmenstrual age, or death. The neurodevelopmental status was assessed at 18-22 months corrected age.Results: The trial was stopped early after enrolling 31% of the projected sample size. Enrolled infants had a median birth weight of 640 g. BPD or death occurred in 21/33 (64%) infants after minimal ventilation and 19/32 (59%) infants after routine ventilation. Minimal ventilation was associated with trends towards higher mortality and higher incidence of neurodevelopmental impairment, and a significantly increased combined outcome of mental impairment or death (p < 0.05).Conclusion: Minimal ventilation as performed in this study did not improve clinical outcome, and may have been associated with a worse neurodevelopmental outcome.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"90 4","pages":"218-25"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000092723","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25991837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 75

Gene expression of the Na-Ca2+ exchanger, SERCA2a and calsequestrin after myocardial ischemia in the neonatal rabbit heart. 新生兔心肌缺血后Na-Ca2+交换器、SERCA2a和钙调磷酸酶的基因表达。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-04-25 DOI: 10.1159/000092888

Matthias Seehase, Thomas Quentin, Elke Wiludda, Gerhard Hellige, Thomas Paul, Holger Schiffmann

{"title":"Gene expression of the Na-Ca2+ exchanger, SERCA2a and calsequestrin after myocardial ischemia in the neonatal rabbit heart.","authors":"Matthias Seehase, Thomas Quentin, Elke Wiludda, Gerhard Hellige, Thomas Paul, Holger Schiffmann","doi":"10.1159/000092888","DOIUrl":"https://doi.org/10.1159/000092888","url":null,"abstract":"Background: Neonatal hearts are less susceptible to developing myocardial dysfunction after hypoxia and/or ischemia than adult hearts. Differences in intracellular calcium homeostasis may be responsible for reduced calcium overload of the immature myocardium leading to the observed protection against ischemia.Objective: To assess differences in baseline and post-ischemic gene expression of calcium handling proteins after ischemia in neonatal and adult rabbit hearts.Methods: We used isolated antegrade perfused rabbit hearts (age 2 days, 28 days, n = 32), which were exposed to ischemia and hypothermia simulating myocardial stunning comparable to neonatal asphyxia. Gene and protein expression of the sodium-calcium exchanger (NCX), the sarco-endoplasmatic reticulum Ca2+-ATPase 2a (SERCA) and calsequestrin (CSQ) were measured using quantitative real-time PCR and Western blotting.Results: After ischemia and reperfusion in neonatal and adult hearts, a significant decrease in myocardial performance was recorded. At the mRNA level, significant differences in the baseline expression of NCX, SERCA and CSQ between neonatal and adult hearts were observed. In neonatal post-ischemic hearts, NCX and CSQ expression were significantly higher at the mRNA level than in controls. In contrast, SERCA expression remained unchanged in neonatal hearts and decreased in adult hearts compared to the non-ischemic controls.Conclusion: These findings suggest that changes in gene expression of calcium handling proteins may be involved in the different susceptibility of neonatal compared to adult hearts to developing myocardial dysfunction after ischemia.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"90 3","pages":"174-84"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000092888","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26000472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Urinary metabolites of oxidative stress and nitric oxide in preterm and term infants. 早产儿和足月儿的氧化应激和一氧化氮的尿代谢产物。

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-05-30 DOI: 10.1159/000093633

Christiana R Farkouh, Jeffrey D Merrill, Phillip L Ballard, Roberta A Ballard, Harry Ischiropoulos, Scott A Lorch

{"title":"Urinary metabolites of oxidative stress and nitric oxide in preterm and term infants.","authors":"Christiana R Farkouh, Jeffrey D Merrill, Phillip L Ballard, Roberta A Ballard, Harry Ischiropoulos, Scott A Lorch","doi":"10.1159/000093633","DOIUrl":"https://doi.org/10.1159/000093633","url":null,"abstract":"Background: Many neonatal diseases have been associated with oxidative stress and altered nitric oxide status.Objective: To determine the effects of clinical interventions on the levels of urinary peroxides, a marker of oxidative stress, and urinary nitrate/nitrites, indices of nitric oxide production and metabolism, in the first 72 h of life in premature infants.Methods: A single, spot urine sample was collected from 82 premature and 20 healthy term infants within the first 72 h of life. The peroxide levels were quantified using a fluorometric method, and nitrate/nitrite levels were quantified by chemiluminescence.Results: Premature infants had a median peroxide level of 10.0 micromol/mmol creatinine (Cr) (interquartile range 4.8-20.0 micromol/mmol Cr). These values were significantly higher than term infants (median 5.0 micromol/mmol Cr, interquartile range 2.7-10.0 micromol/mmol Cr). Urinary nitrate/nitrite levels were not significantly different between preterm (220.5 micromol/mmol Cr, interquartile range 161-287 micromol/mmol Cr) and healthy term infants (244 micromol/mmol Cr, interquartile range 194-316 micromol/mmol Cr). For urinary peroxides, infants on TPN had significantly higher urinary peroxide levels than infants who were not on TPN at the time of urine collection (p = 0.006). Administration of indomethacin was associated with lower levels of urinary nitrate/nitrites (p = 0.0003). Both effects remained significant after controlling for gestational age, degree of respiratory distress and day of urine collection.Conclusion: Monitoring the level of both peroxides and nitrate/nitrite may offer added information about the degree of oxidative stress experienced by a newborn but needs to account for clinical and therapeutic interventions.","PeriodicalId":9091,"journal":{"name":"Biology of the neonate","volume":"90 4","pages":"233-42"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000093633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26054529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17