Proceedings. International Meshing Roundtable最新文献_第8页

Effective Utilization of Marine Fish Industrial Processing Waste Through Co-composting Method for Producing Organic Soil Input 通过共堆肥法有效利用海鱼工业加工废弃物生产有机土壤投入品

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.090090

{"title":"Effective Utilization of Marine Fish Industrial Processing Waste Through Co-composting Method for Producing Organic Soil Input","authors":"","doi":"10.33263/proceedings21.090090","DOIUrl":"https://doi.org/10.33263/proceedings21.090090","url":null,"abstract":"Marine fish processing industries located in the urban region involves fish processing activities that generate a considerable amount of leftover biological residues such as solid wastes and protein-rich effluents, which are ineffectively discarded in water bodies and landfills affecting the coastal environment. The present study involves the biological treatment of industrial fish processing wastes through the co-composting method to convert the fish wastes into organic input. Fish wastes were mixed with rice husk as a bulking agent and allowed for organic matter stabilization through the co-composting process. The maturity of the co-compost was evaluated by spectroscopic techniques, nutrient analysis, and seed germination assay. UV-vis spectroscopic analysis revealed the presence of absorbance between 250 and 300 nm indicating the characteristics of increased concentration of aromatic compounds. The humification index (E4/E6) was calculated to be 1.2, which signified the characteristics of humified materials. Conventional fluorescence spectroscopic analysis detected emission spectra centered at 448 nm due to the development of aromatic structures as a result of increasing the humification process in the co-compost. The well-composted organic matter was found to contain macronutrients (N-1.35%; P-1.02%; K-0.51%) and micronutrients (Zn-74.07 mg/kg; Cu-16.37 mg/kg; Fe-176.07 mg/kg) respectively. Further, seed germination sassay showed growth promotion effects of the co-compost in seeds of Vigna radiata; Vigna mungo and Trigonella foenum-graecum, which confirmed the stability of the organic matter. The findings of the study conclude that the co-composting process of fish industrial processing wastes using rice husk could offer effective conversion of fish waste into organic co-compost for soil application.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77069222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Cytotoxic Activity in Ethanolic Extract of Cucumis melo (L). fruit 甜瓜果实乙醇提取物的细胞毒活性评价

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.070070

{"title":"Evaluation of Cytotoxic Activity in Ethanolic Extract of Cucumis melo (L). fruit","authors":"","doi":"10.33263/proceedings21.070070","DOIUrl":"https://doi.org/10.33263/proceedings21.070070","url":null,"abstract":"Cytotoxicity is the quality of being toxic to cells, and in vitro cytotoxicity testing procedures reduce the use of laboratory animals. The present study was designed to investigate the cytotoxic activity of the Cucumis melo (L) fruit against HepG2 cell lines. To prepare the extract, fresh pulps of Cucumis melo fruit was chopped into pieces and dried at room temperature for 24 hours. 10 g of the dried fruit powder was successively extracted with 100 ml of ethanol using Soxhlet apparatus and filtered through Whatman No 1 filter paper. The cytotoxic activity for cancer cell lines was evaluated by MTT assay. The in vitro cytotoxicity of different concentrations (18.75 - 300μg/mL) of the ethanolic extract of Cucumis melo fruit was evaluated by the MTT assay. The IC50 value is measured by the concentration of extract, causing 50% growth inhibition of cancer cells. The results indicated that the cytotoxic effect of the ethanolic extract of Cucumis melo fruit against HepG2 cells is dose-dependent. At low concentrations, the extract was found to be less toxic towards the HepG2 cells, whereas, at higher concentrations, the toxicity was increased. The concentration at 201.5 µg / ml was found to be an effective dose because, at this concentration, it exhibited 50 % cytotoxicity against HepG2 cells. This work revealed the potentials of ethanolic extract of Cucumis melo fruit as a cytotoxic agent against liver cancer cell lines. The plant can be further screened against various diseases using toxicity models in order to find out its unexplored efficacy.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76074724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Investigation on the in vitro Effects of Elaidic Acid and Gamma-Linolenic Acid on the Survival of MDA-MB-231 and RKO Cell Lines Elaidic酸和γ -亚麻酸对MDA-MB-231和RKO细胞株体外存活影响的研究

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.012012

{"title":"An Investigation on the in vitro Effects of Elaidic Acid and Gamma-Linolenic Acid on the Survival of MDA-MB-231 and RKO Cell Lines","authors":"","doi":"10.33263/proceedings21.012012","DOIUrl":"https://doi.org/10.33263/proceedings21.012012","url":null,"abstract":"Colorectal and breast cancer are a major cause of mortality worldwide. They can be caused due to an array of factors, of which diet plays an important role. Previous studies have suggested that Elaidic acid (EA), a trans fatty acid, supports the growth of colon cancer cell lines, like-RKO, LoVo, and HT29;. In contrast, Gamma-linolenic acid (GLA), a poly-unsaturated fatty acid, has tumoricidal effects. Therefore, the objective of this study was to investigate and delineate the in vitro dose-dependent effects of EA and GLA on the survival of MDA-MB-231(breast cancer cell line) and RKO cells (colon cancer cell line), through cell viability assay. The principal findings of this study were that EA induced a stimulatory effect. At the same time, GLA had an inhibitory effect on both cell lines. There was no statistical significance in the percentage viability of the MDA-MB-231 cells after treatment with EA. At the same time, there was a statistical difference in percentage viability after treatment with GLA. The highest test concentration of GLA that caused approximately 99% inhibition of MDA-MB-231 cells was 500µM, and its IC50 was mathematically calculated to be 239.687µM. For RKO cells, there was a statistical difference in percentage cell viability after treatment with EA. At the same time, there was no statistical difference in percentage cell viability after treatment with GLA. These results suggested that MDA-MB-231 cells were more susceptible to the effects of EA and GLA and also that EA should be consumed in moderation, while GLA appeared as a promising therapeutic option either by itself or in combination with other chemotherapeutic drugs.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"268 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76189299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Colour Stability and Microencapsulation of Anthocyanin Pigment Extracted from Dragon Fruit Peel by Spray and Freeze Drying 喷雾和冷冻干燥法提取火龙果皮花青素色素的颜色稳定性及微胶囊化研究

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.047047

{"title":"Study on Colour Stability and Microencapsulation of Anthocyanin Pigment Extracted from Dragon Fruit Peel by Spray and Freeze Drying","authors":"","doi":"10.33263/proceedings21.047047","DOIUrl":"https://doi.org/10.33263/proceedings21.047047","url":null,"abstract":"Dragon fruit is a popular fruit grown in Malaysia. It is well known for the rich nutrient contents, and it is commercially available worldwide. It is rich in anthocyanins and antioxidants. Dragon fruit is also an essential source of betacyanin, which serves as a red/purple pigment with antioxidative properties. The objective of the present work is to study the stability of natural colorant from dragon fruit peel. The crushed peel and water were taken in the ratio of 1:10 and was subjected to seven different types of extraction techniques such as microwave-assisted extraction, ohmic heating, ultrahigh-pressure homogenization, sonicator, supercritical fluid extraction, aqueous extraction, Soxhlet extraction. Out of the microwave-assisted extraction had the most Anthocyanin content, and it was used for further studies. The selected extract was filtered, and the stability of anthocyanin was examined at varying pH (2…10) and temperature (60…100⁰C). In addition to this, thermal stability (80…100 ⁰C for 150 min) was checked at a deferent time interval (80 to 100⁰C…150 min.). The microwave extract was used for the encapsulation process using maltodextrin, and soy protein isolate as carrier material with 1:4, 1:5 core to wall ratio by spray drying and freeze-drying techniques. The quality analysis, such as total anthocyanin content, encapsulation efficiency, color, water activity, bulk density, solubility, and moisture content, were analyzed of microencapsulated anthocyanin extract. This result showed that encapsulated dragon fruit peel (pitaya) powder could be used as a natural colorant in food applications.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73365989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprospecting Studies of Halopholic Bacteria – Streptomyces sp. MA05 and Halobacterium sp. MA06 嗜盐细菌-链霉菌sp. MA05和盐杆菌sp. MA06的生物勘探研究

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.083083

{"title":"Bioprospecting Studies of Halopholic Bacteria – Streptomyces sp. MA05 and Halobacterium sp. MA06","authors":"","doi":"10.33263/proceedings21.083083","DOIUrl":"https://doi.org/10.33263/proceedings21.083083","url":null,"abstract":"Halophile organisms such as Halobacterium salinarum and halophilic Actinomycetes flourish under the hostile hypersaline condition and are well known for their ability to produce novel bioactive compounds for bioprospecting analysis such as antibacterial, antifungal, anticancer, and enzyme analysis. In this present study, the objective is to isolate two types of halophiles, namely Halobacterium sp. MA06 and Streptomyces sp. MA05 based on their 16SrRNA gene sequencing. The two potential halophiles were isolated from the salt pan of Chennai, India. In order to evaluate enzyme analysis and pigment production of both the organism, optimization of the growth state of both species was performed on complex medium with various additives and different concentrations of sodium chloride and magnesium sulfate. The result showed that Halobacterium sp. MA06 produced orange colored pigment after characterization using GC-MS analysis. For Streptomyces sp. MA06, it was found to produce the enzyme amylase, hence Streptomyces sp. MA06 was subjected to producing the enzyme amylase, and the produced amylase will be characterized. The antimicrobial and anticancer activity was then carried out on the extract of Streptomyces sp. MA05 and the pigment of Halobacterium sp. MA06. In conclusion, the pigment from Halobacterium sp. MA06 and the enzyme amylase from Streptomyces sp. MA05 could be a novel bio-sourced for bioprospecting of bioactive natural products.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74757219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiofilm and antioxidant activity of formulated nanoemulsion from Ocimum tenuiflorum 山茱萸纳米乳的抗生物膜及抗氧化活性研究

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.007007

{"title":"Antibiofilm and antioxidant activity of formulated nanoemulsion from Ocimum tenuiflorum","authors":"","doi":"10.33263/proceedings21.007007","DOIUrl":"https://doi.org/10.33263/proceedings21.007007","url":null,"abstract":"Nanoemulsions are nano-sized emulsions, which are made for improving the delivery of active pharmaceutical ingredients. These are the thermodynamically steady isotropic system where two immiscible liquids are mixed to form a single-phase by means of an emulsifying agent, i.e., surfactant and co-surfactant. In this study, the extracts of essential oils from medicinal plants, namely, Ocimum tenuiflorum (Tulasi) is used, which have antimicrobial, antioxidant, antibiofilm, and anti-inflammatory activities. Oil in water emulsions was prepared by high-pressure homogenizer using plant essential oils with water as a dispersion medium. The physical and mechanical stability tests were carried out. The emulsions were subjected to antibiofilm assays, and results were compared to estimate the anti-biofilm efficacy of the emulsions. The emulsions were prepared by varying concentrations and time to determine the particle size distribution. These results proposed for developed plant essential oil-based nanoemulsion is thermodynamically stable, and formulated nanoemulsion will be used in various biomedical applications, textile industry applications, and food industry.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80693436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymer Extraction from Lignocellulosic Biomass (Water Hyacinth) 从木质纤维素生物质(水葫芦)中提取聚合物

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.077077

{"title":"Polymer Extraction from Lignocellulosic Biomass (Water Hyacinth)","authors":"","doi":"10.33263/proceedings21.077077","DOIUrl":"https://doi.org/10.33263/proceedings21.077077","url":null,"abstract":"Bioconversion of renewable lignocellulosic biomass to biofuel and value-added products is globally gaining significant importance. Lignocellulosic wastes are the most promising feedstock considering its great availability and low cost. The biomass conversion process involves mainly two steps: hydrolysis of cellulose in the lignocellulosic biomass to produce reducing sugars and fermentation of the sugars to ethanol and other bioproducts. However, sugars necessary for fermentation are trapped inside the recalcitrant structure of the lignocellulose. Hence, pretreatment of lignocellulosic wastes is always necessary to alter and/or remove the surrounding matrix of lignin and hemicelluloses in order to improve the hydrolysis of cellulose. These pretreatments cause physical and/or chemical changes in the plant biomass in order to achieve this result. Each pretreatment has a specific effect on the cellulose, hemicellulose, and lignin fraction. Thus, the pretreatment methods and conditions should be chosen according to the process configuration selected for the subsequent hydrolysis steps. In general, pretreatment methods can be classified into four categories, including physical, physicochemical, chemical, and biological pretreatment. Bioresource utilization of biopolymeric materials has now gained recent attention. Cellulose was extracted from water hyacinth by acid, alkali treatment & extracted cellulose was grafted with curcumin, pesticide, grape juice, magnetorheological fluid, and the grafted composite material was evaluated for release of respective grafted materials. In the present study, a polymer extracted from water hyacinth was evaluated for various applications. The present study would suggest the possible utilization of water hyacinth composite as the biomaterial for diverse applications.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85728067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective Utilization of Pineapple Waste, Evaluation of Ferulic Acid, Micro Nutrients and Antioxidant Property 菠萝废弃物的有效利用、阿魏酸、微量营养素及抗氧化性能评价

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.030030

{"title":"Effective Utilization of Pineapple Waste, Evaluation of Ferulic Acid, Micro Nutrients and Antioxidant Property","authors":"","doi":"10.33263/proceedings21.030030","DOIUrl":"https://doi.org/10.33263/proceedings21.030030","url":null,"abstract":"India is the second-largest producer of fruit and vegetable producers. Usually, after fruit intake, fruit peel is left as waste. The main bioactive components of pineapple are phenolic compounds, β-carotene, ascorbic acid, and flavonoids. Ferulic acid is a phenolic acid widely used in the nutritional and cosmetic fields. In this study, pineapple peel was dried, powdered, and vitamin content (A, B, B1, B2, B6, B12 & C), calcium, potassium, phosphorus, iron, manganese, zinc and food fiber were analyzed. Moreover, in the present study, traditional and non-conventional processes such as Soxhlet extraction, supreme fluid extraction, and normal solvent extraction was used for the extraction of ferulic acid, which is a precursor for vanillin synthesis. The quantification of ferulic acid was done by High performance fluid chromatographic (HPL C) method. After the above-mentioned extraction process overall phenolic and antioxidant activity were also evaluated and compared. The highest concentrations of ferulic acid (0.7696g/100 g), phenolic compound (2.365mg / GAE), antioxidant activity (45 percent), and yield (90.5 %) were obtained for Soxhlet extraction using methanol.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"144 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73399295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theranostic Approaches in Microbiology: A Paradigm Shift in Therapeutic and Diagnostic Advents 微生物学的治疗方法:治疗和诊断进展的范式转变

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.084084

引用次数: 0

Zinc Chloride Negatively Impacts Early Embryonic Development as Assessed using Human Embryonic Stem Cells as a Model 以人胚胎干细胞为模型评估氯化锌对早期胚胎发育的负面影响

Proceedings. International Meshing Roundtable Pub Date : 2020-07-15 DOI: 10.33263/proceedings21.022022

{"title":"Zinc Chloride Negatively Impacts Early Embryonic Development as Assessed using Human Embryonic Stem Cells as a Model","authors":"","doi":"10.33263/proceedings21.022022","DOIUrl":"https://doi.org/10.33263/proceedings21.022022","url":null,"abstract":"Electronic cigarettes (ECs) or vaping products are nicotine delivery devices that have gained significant acceptance recently. EC or vaping product use associated lung injury (EVALI) has raised awareness regarding the damage caused by vaping. ECs expose the user and embryo/fetus to nicotine, flavor chemicals, solvents, metals, and reaction products. Little is known about how these chemicals affect prenatal development. Our prior work has shown that zinc is elevated in most EC aerosols. Our objective was to examine the effect of zinc on H9 human embryonic stem cell (hESC) colonies using live-cell imaging and to further determine if mitochondria were affected by zinc. hESC were plated, then treated with zinc chloride for 24 hours, after which the hESC were washed and labeled using Mitotracker deep red and imaged for 48 hours in a BioStation CT. StemCell QC was used to extract features from live-cell imaging data. We are using software such as MitoMo and CL Quant to examine the morphology of the mitochondria and analyze them. We observed that the mitochondria that were stressed would affect cell functionality and health. Stressed mitochondria will subsequently affect embryonic development; hence pregnant women should avoid using ECs during early pregnancy when the embryo is most susceptible to toxicants.","PeriodicalId":90703,"journal":{"name":"Proceedings. International Meshing Roundtable","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73905460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0