Bioprospecting Studies of Halopholic Bacteria – Streptomyces sp. MA05 and Halobacterium sp. MA06

Abstract

Halophile organisms such as Halobacterium salinarum and halophilic Actinomycetes flourish under the hostile hypersaline condition and are well known for their ability to produce novel bioactive compounds for bioprospecting analysis such as antibacterial, antifungal, anticancer, and enzyme analysis. In this present study, the objective is to isolate two types of halophiles, namely Halobacterium sp. MA06 and Streptomyces sp. MA05 based on their 16SrRNA gene sequencing. The two potential halophiles were isolated from the salt pan of Chennai, India. In order to evaluate enzyme analysis and pigment production of both the organism, optimization of the growth state of both species was performed on complex medium with various additives and different concentrations of sodium chloride and magnesium sulfate. The result showed that Halobacterium sp. MA06 produced orange colored pigment after characterization using GC-MS analysis. For Streptomyces sp. MA06, it was found to produce the enzyme amylase, hence Streptomyces sp. MA06 was subjected to producing the enzyme amylase, and the produced amylase will be characterized. The antimicrobial and anticancer activity was then carried out on the extract of Streptomyces sp. MA05 and the pigment of Halobacterium sp. MA06. In conclusion, the pigment from Halobacterium sp. MA06 and the enzyme amylase from Streptomyces sp. MA05 could be a novel bio-sourced for bioprospecting of bioactive natural products.