Journal of mammalian ova research最新文献_第3页

Ethical and Social Implications of Current Prenatal Genetic Testing 当前产前基因检测的伦理和社会影响

Journal of mammalian ova research Pub Date : 2016-10-17 DOI: 10.1274/jmor.33.109

Azumi Tsuge

引用次数: 1

Challenges Towards Establishing Germline Gene Therapy for Inherited Mitochondrial Diseases 建立遗传性线粒体疾病种系基因治疗的挑战

Journal of mammalian ova research Pub Date : 2016-10-17 DOI: 10.1274/jmor.33.89

Naomi Shiga, Masahito Tachibana, N. Yaegashi

引用次数: 1

A Review: the Essentials of Informed Consent for Cancer Patients Before Autologous Transplantation of Ovarian Tissue 肿瘤患者自体卵巢组织移植前知情同意的要点综述

Journal of mammalian ova research Pub Date : 2016-10-17 DOI: 10.1274/jmor.33.115

K. Kyono, T. Hashimoto, M. Kuchiki, E. Sakamoto, Masayo Tani, Atsuko Tanaka, Sachiko Nonaka, Yusuke Nakamura, Y. Nakajo, N. Aono, T. Takeuchi

{"title":"A Review: the Essentials of Informed Consent for Cancer Patients Before Autologous Transplantation of Ovarian Tissue","authors":"K. Kyono, T. Hashimoto, M. Kuchiki, E. Sakamoto, Masayo Tani, Atsuko Tanaka, Sachiko Nonaka, Yusuke Nakamura, Y. Nakajo, N. Aono, T. Takeuchi","doi":"10.1274/jmor.33.115","DOIUrl":"https://doi.org/10.1274/jmor.33.115","url":null,"abstract":"Abstract: Purpose: To highlight the imperative of informed consent in the fertility preservation of cancer patients before ovarian tissue autotransplantation. Methods: Three papers of tumor recurrence after autotransplantation of frozen-thawed ovarian tissue were compared with the main papers before tumor recurrence was reported in the cancer patients. Results: Histology was performed before autotransplantation in cases 1 and 3, but not in case 2. Histology alone is insufficient for the detection of minimal residual disease (MRD). Furthermore, transplanted ovarian tissue is different from that examined for MRD detection, and how much of the resected ovarian tissue was examined for MRD detection is unclear. The possibility that grafted tissue caused tumor recurrence cannot be ruled out in any of the three cases, because autotransplantation in cancer patients, at present, uses ovarian tissue different from that examined for MRD. Conclusions: We recommend informed consent for cancer patients, because: 1) the transplanted ovarian tissue is different from the ovarian tissue examined for MRD detection; 2) the amount of resected ovarian tissue analyzed for MRD is very small; and 3) MRD detection methods vary. In conclusion, freezing and storage of ovaries should be encouraged, transplantation must be performed carefully, and informed consent is essential.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"29 1","pages":"115 - 118"},"PeriodicalIF":0.0,"publicationDate":"2016-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88551800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Estimating the Survival Probability of Nuclear-Transfer Embryos before Embryo Transfer by a Novel Biopsy: Oct4 and Sox2 Gene Expression Patterns of a Monozygotic Twin Blastocyst Separated at the 2-Cell Stage of Nuclear-Transfer Embryos 通过一种新的活检方法估计核移植胚胎在胚胎移植前的存活率:在核移植胚胎2细胞期分离的单卵双囊胚的Oct4和Sox2基因表达模式

Journal of mammalian ova research Pub Date : 2016-05-26 DOI: 10.1274/jmor.33.55

Kazuki Ohata, Y. Kato

引用次数: 2

The Early History of the TYH Medium for in vitro Fertilization of Mouse Ova TYH培养基用于小鼠卵子体外受精的早期历史

Journal of mammalian ova research Pub Date : 2016-05-26 DOI: 10.1274/jmor.33.3

Y. Toyoda, M. Yokoyama

引用次数: 16

Human Preimplantation Embryo Culture Media: Past, Present, and Future 人类着床前胚胎培养基:过去、现在和未来

Journal of mammalian ova research Pub Date : 2016-05-26 DOI: 10.1274/jmor.33.17

Tatsuma Yao, Yuta Asayama

{"title":"Human Preimplantation Embryo Culture Media: Past, Present, and Future","authors":"Tatsuma Yao, Yuta Asayama","doi":"10.1274/jmor.33.17","DOIUrl":"https://doi.org/10.1274/jmor.33.17","url":null,"abstract":"Abstract: \u0000 Systematic studies of mouse embryo culture beginning in 1949 led to an understanding of essential medium components for early mammalian embryos, and embryo culture from the zygote to the blastocyst stage was achieved in 1968. Since then, medium components that are either beneficial or detrimental for embryo culture have been identified. A variety of culture media that mimic the female reproductive tract, such as human tubal fluid medium and sequential media, were developed from the 1970s to the 1990s, and a single medium in which the concentrations of components were determined by a simplex optimization method was introduced for clinical use in 2002. While either sequential media or a single medium is currently used in most cases, no conclusion has yet been reached as to which of the two approaches is the best. That we are now easily able to culture embryos is the result of the work of pioneers. This review presents a chronological overview of media development from initial attempts at mouse embryo culture using synthetic media to the human embryo culture media used today. It also presents the characteristics of sequential media and a single medium. Finally, problems observed with current embryo culture media are discussed, along with future development in this area.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"189 1","pages":"17 - 34"},"PeriodicalIF":0.0,"publicationDate":"2016-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86233093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Incidence of Apoptotic Cells After Vitrification in Canine Ovarian Tissues 犬卵巢组织玻璃化后细胞凋亡的发生率

Journal of mammalian ova research Pub Date : 2016-05-26 DOI: 10.1274/jmor.33.69

Madoka Hariya, Hiroshi Suzuki

{"title":"Incidence of Apoptotic Cells After Vitrification in Canine Ovarian Tissues","authors":"Madoka Hariya, Hiroshi Suzuki","doi":"10.1274/jmor.33.69","DOIUrl":"https://doi.org/10.1274/jmor.33.69","url":null,"abstract":"Abstract: Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported, the degree of cryoinjury after the vitrification has not yet been fully examined. Since cryoinjury after vitrification of ovaries has been reported in bovine and humans, this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL assay. Ovaries were collected from immature bitches. The ovarian tissue cubes were pretreated with 1 M DMSO. Subsequently, DAP 213 solution (2 M DMSO, 1 M acetamide, 3 M propylene glycol) was added. The cryotubes containing the ovarian tissues were placed on ice for 5 min, then plunged directly into liquid nitrogen. After warming with 0.25 M sucrose, cryopreserved ovarian tissues were fixed and subsequently stained with hematoxylin-eosin, proliferation cell nuclear antigen (PCNA) antibody, or active caspase-3 (AC-3) antibody, and evaluated by the TUNEL assay. There were no differences in the numbers of primordial, primary, secondary and antral follicles/mm2 between the fresh and vitrified-warmed ovarian tissues. Percentages of PCNA and AC-3 antibody positive cells were similar regardless of the developmental stage of the follicles and experimental group. A few TUNEL positive cells were detected in both experimental groups. These results suggest that vitrification with DAP213 does not induce cryoinjury in ovarian tissues from immature bitches.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"24 1","pages":"69 - 75"},"PeriodicalIF":0.0,"publicationDate":"2016-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84561695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Development of a Culture Medium for Rat 1-Cell Embryos 大鼠1细胞胚胎培养基的研制

Journal of mammalian ova research Pub Date : 2016-05-26 DOI: 10.1274/jmor.33.11

K. Miyoshi

引用次数: 5

Towards Further Optimization of Preimplantation Embryo Culture Media: from the Viewpoint of Omics and Somatic Cell Nuclear Transfer (SCNT) Studies 进一步优化着床前胚胎培养基:从组学和体细胞核移植研究的角度

Journal of mammalian ova research Pub Date : 2016-05-26 DOI: 10.1274/jmor.33.35

Mitsutoshi Yamada, T. Hamatani, H. Akutsu, Mamoru Tanaka

引用次数: 0

Microarray Analysis of Differentially Expressed Genes in Inner Cell Mass and Trophectoderm of Parthenogenetic Embryos 孤雌生殖胚胎内细胞团和滋养外胚层差异表达基因的微阵列分析

Journal of mammalian ova research Pub Date : 2016-05-26 DOI: 10.1274/jmor.33.45

Shiori Goto, F. Cao, T. Kono, H. Ogawa

{"title":"Microarray Analysis of Differentially Expressed Genes in Inner Cell Mass and Trophectoderm of Parthenogenetic Embryos","authors":"Shiori Goto, F. Cao, T. Kono, H. Ogawa","doi":"10.1274/jmor.33.45","DOIUrl":"https://doi.org/10.1274/jmor.33.45","url":null,"abstract":"Abstract: \u0000 Mouse parthenogenetic (PG) embryos do not survive beyond day 9.5 of pregnancy. In this study, to understand the molecular mechanisms underlying the failure of parthenogenesis at the early developmental stage, we performed global gene expression profiling of the PG inner cell mass (ICM) and trophectoderm (TE) using microarray analysis, compared the results with those from in vitro-fertilized embryos, and identified genes whose expression levels showed more than a 4-fold change as a cutoff. Eighty probe sets were up-regulated and 59 probe sets down-regulated in the PG ICM, while 169 and 43 probe sets were respectively up-regulated and downregulated in PG TE. We selected two genes (Sfmbt2 and Gab1) that were down-regulated in both the PG ICM and TE, one gene (Nat1) that was down-regulated in the PG ICM, and one gene (Lysmd2) that was up-regulated in the PG TE, and analyzed the gene expression levels using real-time PCR. The quantitative expression levels of these four genes were confirmed by real-time PCR. In the present study, we identified differentially expressed genes in PG embryos and also identified those that were ICM- or TE-specific in PG embryos.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"16 1","pages":"45 - 54"},"PeriodicalIF":0.0,"publicationDate":"2016-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87634244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1