{"title":"Quantification of Heavy Metal Accumulation in Water and Edible Fish Tissue Samples Collected From the River Tawi, Jammu, India","authors":"M. Sharma, R. Gandotra, S. Jangral","doi":"10.3329/jbs.v29i2.54951","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54951","url":null,"abstract":"The present study estimated the concentration (in ppm) of heavy metals of Zn, Fe, Cu and Pb in water samples and edible tissue of Labeo boga; both collected from the non polluted (Station I) and polluted (Station II) locations of river Tawi, Jammu, India. The mean concentration of heavy metals (Zn, Fe, Cu, Pb) at Station I were 0.048±0.008 (ppm); 0.016±0.001 (ppm); 0.157±0.002 (ppm) and 0.0001±0.0001 respectively; while at Station II, these values were 0.133±0.001 (ppm); 0.022±0.002 (ppm); 0.206±0.002 (ppm) and 0.002±0.001 (ppm) respectively; the statistical difference between the stations were significantly higher (p<0.05). The order of heavy metal load obtained in water samples at both the stations was found to be Fe>Zn>Cu>Pb. Also, the mean bioaccumulation of heavy metals viz. Zn, Fe, Cu and Pb in the edible tissue of fish at Station I was found to be 0.374±0.011 (ppm), 0.105±0.02 (ppm); 0.094±0.04 (ppm) and0.001±0.0001 (ppm) respectively; on the other hand, mean bioaccumulation was 0.539±0.013 (ppm); 0.156±0.04 (ppm); 0.121±0.023 (ppm) and 0.0013±0.0001 (ppm) respectively at Station II. Even though bioaccumulation of the heavy metals in the experimental sites did not exceed the acceptable limits recommended by the agencies like FAO and WHO, the present results showed an increasing trend of the accumulation, which definitely poses a serious threat to the survival of aquatic organisms in the study area. J. Bio-Sci. 29(2): 19-29, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ahsan, Mt Alam, M. Chowdhury, M. Nasim, S. Islam
{"title":"In Vitro and in Vivo Evaluation Of Pharmacological Activites of Pouzolzia Sanguinea","authors":"M. Ahsan, Mt Alam, M. Chowdhury, M. Nasim, S. Islam","doi":"10.3329/jbs.v29i2.54952","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54952","url":null,"abstract":"Pouzolzia sanguinea grows in tropical and sub-tropical regions of Bangladesh, used for a variety of purposes including pain, rheumatoid kidney diseases in traditional medicine. The crude ethanolic leaf extract of P. sanguinea with its different fractions (ethanol, n-hexane, and chloroform) was investigated for phytochemical constituents, in vitro antioxidant, anti-inflammatory effects, in-vivo analgesic and antipyretic activities. Preliminary phytochemical constituents were identified by chemical group test. P. sanguinea fractionated extracts contain alkaloids, glycosides, tannins, flavonoids, saponins, gums, and amides. Antioxidant activity test was performed by both qualitative (TLC and Rf value) and quantitative tests (inhibition of DPPH free radical scavenging). Extracts exhibited significant (p <0.001, p <0.0001) inhibition of DPPH free radical scavenging activity as compared to the standard drug ascorbic acid at similar doses. In vitro anti-inflammatory activity was determined by protein denaturation of egg albumin method. The percent inhibition of protein denaturation in the experiment of ethanol extract was found significantly higher (p <0.0001) compared with chloroform and n-hexane extracts. In addition, in vivo analgesic and antipyretic effects were determined in mice by acetic acid-induced writhing and yeast-induced pyrexia methods. The ethanol extracts of P. sanguinea exhibited inhibition of writhing reflex on mice by 71.58% at the dose of 500 mg/kg body weight which had greater analgesic activity than other n-hexane and chloroform extracts. In the anti-pyretic test, fractional extracts ethanol, chloroform, and n-hexane at a dose of 500 mg/kg body weight significantly (p <0.05) decreased pyrexia in mice up to 3 h as compared with the positive control paracetamol drug at a dose of 150 mg/kg body weight. In our in vitro and in vivo study models, it is evident that Pouzolzia sanguinea fractionated extracts showed significant antioxidant, anti-inflammatory, analgesic, and antipyretic activities. J. Bio-Sci. 29(2): 31-42, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Khatun, Radinal Raka, M. Islam, M. Hossain, M. Rasel, M. Mondol
{"title":"Inclusion of Bivalve in Carp Polyculture Ponds Managed by Small Indigenous People Under Barind Tract","authors":"T. Khatun, Radinal Raka, M. Islam, M. Hossain, M. Rasel, M. Mondol","doi":"10.3329/jbs.v29i2.54959","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54959","url":null,"abstract":"The present experiment was carried out in the household ponds (0.04 to 0.08 ha in area with 1.3 to 2.4 m in depth) of small indigenous people at Barind Tract region, northwest Bangladesh during September, 2017 to June, 2018 to know the growth performance of bivalve with carp. Two different treatments with 3 replicates were used, in treatment one (T1) only the carp polyculture was performed and in treatment two (T2) carp and bivalve polyculture was performed. Good quality of carp fingerlings (Labeo rohita, Gibelion catla, Cirrhinus cirrhousus, Hypophthalmichthys molitrix and Ctenopharyngodon idella) and freshwater bivalve (Lamellidens marginalis) seeds were stocked in the ponds. Stocking density and size of the seeds were similar for all of the study ponds. During the experiment there were no significant differences was observed in growth performance of the fishes between the two different treatments (p<0.05). The weight gain was recorded for L. rohita 420.31 g in T1 and 436.25 g in T2, for G. catla 237.01 g in T1 and 329.66 g in T2, for C. cirrhosus 263.53 g in T1 and 301.74 g in T2, for H. molitrix 559.6 g in T1 and 593.57 g in T2, for C. idella 510.11 g in T1 and 619.57g in T2. The weight gain of L. marginalis was 12.3 g in T2. During this experiment the production of carp was recorded 2524.5 kg/ha in T1 and 2618.2 kg/ha in T2 and the bivalve production was 1284.4 kg/ha in T2. This study is the first report on growth performance of bivalve with carp in the Barind Tract region of northwest Bangladesh. The result of this study will be helpful for the development of bivalve aquaculture in this climatically vulnerable region. J. Bio-Sci. 29(2): 99-110, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SC Tasneem, J. Ferdous, Mzh Bulbul, M. Misbah, D. Sujan, I. Hasan, S. Kawsar
{"title":"Antimicrobial and Anticancer Activities of Some Partially Acylated Thymidine Derivatives","authors":"SC Tasneem, J. Ferdous, Mzh Bulbul, M. Misbah, D. Sujan, I. Hasan, S. Kawsar","doi":"10.3329/jbs.v29i0.54818","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54818","url":null,"abstract":"The principal cause of human deaths in the world is primarily due to harmful pathogenic microbes as bacteria, a virus, and fungi. Searching for antimicrobial agents to prevent deaths with new structures and modes of action is an essential strategy of the research. Therefore, in the present study has been undertaken of thymidine and of its eleven acylated derivatives were employed as test chemicals for in vitro antibacterial evaluation against five human pathogenic bacteria. The compounds were also screened for antifungal functionality tests against two phytopathogenic fungi. It was observed that a good number of tested compounds exhibited moderate to good antimicrobial activities. It was also observed that these acylated compounds were more effective against the phytopathogenic fungi than those of the bacterial strains. Encouragingly, several tested chemicals showed better antibacterial and antifungal activities than the standard antibiotics employed. The minimum inhibition concentration (MIC) values of the compounds 3 (5´-O-butyrylthymidine), 7 (5´-O-palmitoylthymidine) and 12 (5´-O-4- dichloroacetylthymidine) were found to be 0.32 mg/ml and minimum bactericidal concentrations (MBC) values were found to be 0.63 mg /ml in each case. On the other hand, the minimum fungal concentration (MFC) was found to be (1.25 mg/ml) in the case of compound 7 (i.e., palmitoyl derivative) which may be used as antifungal active drug providing further investigation. In vitro MTT assays revealed that compounds 4 (5´-O-hexanoylthymidine) and 7 (5´-O-palmitoylthymidine) were effective against Ehrlich’s ascites carcinoma (EAC) cells and IC50 values were found to be 920.88 μg/ml and 792.90 μg/ml, respectively. So these compounds may be targeted for future studies for their usage as broad-spectrum antibiotics. J. Bio-Sci. 29(1): 11-22, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphometric Characters and Sex Ratio of the Freshwater Garfish, Xenentodon Cancila Collected From Different Waters of Jashore, South-Western Bangladesh","authors":"M. Islam, P. Laboni","doi":"10.3329/jbs.v29i0.54819","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54819","url":null,"abstract":"The freshwater garfish, Xenentodon cancila is one of the important food fish in Bangladesh. The size class distribution, length-weight relationships, and sex ratio of X. cancila are described in the present study. A total of 138 specimens were collected randomly for a year from the local fish markets and/or direct from fishermen who fish primarily in nearby waters (Beel, Canal and Baor) of Jashore, southwestern Bangladesh. Most individuals were collected in the rainy season and their mean size was larger compare to the other two seasons (summer and winter). The maximum size recorded 254.1 mm total length (TL) and 38.05 g body weight (BW). The mean TL and BW were 166.35±38.17 mm and 11.16±7.47g, respectively. Most of the individuals belonged to the size class of 141-180 mm. The length-weight relationship (LWR) was strongly correlated as the regression value was 0.876 and the relationships were linear in both sexes. The allometric coefficient ‘b’ of the LWR indicated nearly positive allometric growth and the value was 2.98. The male fishes were dominant over females and the ratio was 1: 0.77. These research outcomes would be useful for the researcher to better understand the biological aspects of freshwater garfish in the nearby waters of Jashore area in south-western Bangladesh. J. Bio-Sci. 29(1): 23-31, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Siddique, T. Asa, M. Sohag, M. Chowdhury, A. Iqbal, K. Islam
{"title":"Molecular Diagnosis and Evolutionary Relationship Analysis Of Plant Parasitic Tea Garden Nematodes From Different Tea Estates In Sylhet Region of Bangladesh","authors":"M. Siddique, T. Asa, M. Sohag, M. Chowdhury, A. Iqbal, K. Islam","doi":"10.3329/jbs.v29i0.54826","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54826","url":null,"abstract":"Nematodes from plant-parasitic sources are ever-present and incidental to plant growth as well as crop production. The damage of tea gardens caused by nematode is often non-specific and easily confused with symptoms. The present study determined the parasitic and non-parasitic nematodes population in different tea gardens of the Sylhet region by their morphological and partial molecular characterization. Out of 13 tea gardens, it was observed that BTRI, Karimpur, Mathiura, and Tarapur tea garden has the highest number of parasitic and non-parasitic nematodes. After PCR amplification, DNA bands with desired amplicon size were detected by gel electrophoresis. Among thirteen soil samples, nematodes from Malnichara, Karimpur, BTRI, Mathiura , and Finlay had partially confirmed the presence of rootknot nematode (Meloidogyne spp.), root-lesion nematode (Pratylenchus brachyurus), burrowing nematode (Radopholus similis), reniform nematode (Rotylenchulus reniformis) and lance nematode (Hoplolaimus columbus) consequently based on approximately base pair of 1.7, 1.1 and 0.52 kb (different Meloidogyne spp.) 0.52, 0.52, 0.25 and 2.3 kb of specific genes. From evolutionary analysis, it might be said that Meloidogyne species are strongly related with each other making clusters except Meloidogyne natalie where this one is closely related with Hoplolaimus columbus in their evolutionary relationship as remaining others (Rotylenchulus reniformis, Radopholus similis, Pratylenchus brachyurus) are in different clusters in the same clade and this result could be confirmed after sequencing. J. Bio-Sci. 29(1): 101-109, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation Between Oxidative Stress Generation, Reticular Fiber Density and Testicular Histomorphometric Parameters Of Streptozotocin-Induced Diabetic Wistar Rat","authors":"G. Anyanwu, Ca Agbor","doi":"10.3329/jbs.v29i2.54949","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54949","url":null,"abstract":"The actual underlying mechanism of alterations in testicular histomorphometric parameters resulting from diabetes-induced oxidative stress is still not well understood because of the absence of supporting evidence from scientific experiments. This study was conducted to investigate the correlation between serum oxidative stress markers with testicular histomorphometric parameters and reticular fiber density of Streptozotocin-induced diabetic Wistar rat. The experiment included twenty eight adult male rats sorted into four groups, Group A (Control), other animals were sorted into treatment groups based on their blood glucose levels after inducing diabetes with 65 mg/kg/bw of streptozotocin, Groups B (100 - 200 mmol/l), Group C (210 - 250 mmol/l), Group D (260 - 300 mmol/l). At termination, Serum superoxide dimutase (SOD), catalase and melondialdehyde where evaluated using reagent based antioxidant enzyme assay while reticulum stain kits was used to demonstrate for reticular fiber density. Histomorphometric measurements were carried out using ocular micrometer after calibration on a light microscope. Statistical analysis was done using analysis of variance with p<0.05 considered significant. Results reveal that the higher the blood glucose levels in diabetic animals, the higher the serum concentration of oxidative stress markers. Density of reticular fiber increased with increase in blood glucose levels, while tubular diameter and epithelial height decreased with increase in increase in hyperglycaemic levels. In conclusion, there was a progressive increase in reticular fiber density and decrease in tubular diameter and epithelial height as a consequence of increase oxidative stress generation in diabetic model. J. Bio-Sci. 29(2): 01-08, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hoque, M. Mondal, G. Sagor, M. Hasan, A. Hannan
{"title":"Inclusive Scenario of Natural Products Obtained From Agar Plant (Aquilaria SP.) In Bangladesh- A Review","authors":"M. Hoque, M. Mondal, G. Sagor, M. Hasan, A. Hannan","doi":"10.3329/jbs.v29i2.54963","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54963","url":null,"abstract":"Agarwood is the resinous heartwood obtained from the injured parts of trees under Thymelaeaceae family particularly from Aquilaria species. Agarwood is considered as the most prized non-timber forest product (NTFP) used in attars as well as medicines. Quality of agarwood is the determiner for defining its commercial value. Different countries use different grading system to explain the quality of agarwood. In Bangladesh, more than 45 compounds have been identified so far, mostly sesquiterpenoids, alkanes, fatty acid and other volatile aromatic compounds that are responsible for its fragrance and high prices. Ether extract, total phenolic contents, flavonoid, antioxidant and microbiological tests have shown tremendous positive results. Chemical profiling of agar products is the best identified using GC-MS technique by the various authors. Present review discusses the full scenario of Aquilaria production, inoculation, extraction methods, determination of agarwood quality and chemical constituents of agar oils and possibilities and barriers of this industry in Bangladesh. J. Bio-Sci. 29(2): 151-173, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic diversity analysis based on morphological characters in mulberry (Morus spp.)","authors":"MS Rahman, S. Islam","doi":"10.3329/jbs.v28i0.44717","DOIUrl":"https://doi.org/10.3329/jbs.v28i0.44717","url":null,"abstract":"Mulberry genetic resource is increasingly being recognized as one of the basic key component for sustainable silk production under changing climatic condition. In this investigation, analysis of multivariate was done to assess the diversity in 20 mulberry genotypes (includes indigenous and exotic) for leaf yield and its growth attributes. The analysis of variance (ANOVA) showed the presence of significant variation among genotypes for the parameters measured. Wide range and variance among the genotypes indicated the presence of variability for the traits on which selection can be practiced. For cluster analysis classified 20 genotypes into four divergent groups and greater genetic distance was detected among the members of cluster I and II and cluster II and III. The members of these divergent clusters may be combined in future breeding programmes to obtain genotypes with combined leaf yield and more branches per plant. The results showed that the germplasm having a wide genetic diversity thus the genotypes viz., BSRM64, BSRM66, BSRM63, BSRM65, BSRM45 and BSRM56 can serve as a promising donors for improving the leaf productivity of mulberry. \u0000J. bio-sci. 28: 111-119, 2020","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"28 1","pages":"111-119"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v28i0.44717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46013397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantification of heavy metals in canned tomato paste sold in Ubani-Umuahia, Nigeria","authors":"RI Uroko, VE Okpashi, N. Etim, AC Fidelia","doi":"10.3329/jbs.v28i0.44705","DOIUrl":"https://doi.org/10.3329/jbs.v28i0.44705","url":null,"abstract":"In recent years there has been an increase in the contaminations of heavy metals on the environment. Government and private organization have shown their interest in the effect of dietary exposure to several heavy metals. These heavy metals have been implicated in the etiology of many diseases with high risk to humans. Canned tomatoes paste is one of the important health deterioting factors to human health in Ubani-Umuahia, Nigeria. In this study atomic absorption spectrophotometer was used to screen for nine heavy metals concentration in ten different brands of canned tomatoes paste sold at Ubani-Umuahia market in Nigeria. Human risk assessment was calculated using the collated data to evaluate the predictive risk of human health after the consumption of canned tomatoes paste. Results shows that lead and nickel were not detected in all the canned tomatoes. In comparison low concentrations of copper, iron, and manganese were notated but high concentrations of chromium and cadmium were detected in all the tested tomatoes pastes. Cobalt and zinc concentration was notated lower than permissible limit. The daily intake of copper, cobalt, manganese, chromium, cadmium, nickel, iron, zinc and lead were below their tolerable values in canned tomatoes. The predicted lifetime for carcinogens to occur was less than one (<1). Copper, cobalt, iron, and zinc were below the acceptable value for non-cancer risk with HQ <1.The risk of incurring cancer by ingesting canned tomatoes was within the lifetime predicted a range of (1.0E-6 to 1.0E-4). Finds suggest that prolong and persistent consumption of these heavy metals may cause toxicity and consequential heath challenges. \u0000J. bio-sci. 28: 1-11, 2020","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v28i0.44705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49247095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}