Journal of bio-science最新文献

{"title":"Studies on Food, Health and Environmental Perspectives In Mulberry (Morus Spp.) – A Review","authors":"MS Rahman, S. Islam","doi":"10.3329/jbs.v29i0.54832","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54832","url":null,"abstract":"Mulberry is a very hard and quickly developing plant belongs to the family of Moraceae. The leaf of mulberry is exclusively utilized for feeding and rising of the silkworm (Bombyx mori) for the production of silk yarn. It is evaluated that mulberry silk contributes around 90% of the total worldwide raw silk production that contributed to socio-economic development worldwide. The usage of mulberry leaves as silkworm feed, it is being utilized for food and medicinal purpose also. For instance, the mulberry fruit because of its high nutritive value and flavorful taste is getting significance as important foodstuff. The mulberry bark and wood are likewise helpful for assembling of paper and sports products things. Mulberry is a quickly developing with profound roots, flourish leaves, high protections from pollution, wind, sand, drought, and salinity with strong adaptability and simple cultivation. The broad environmental adaptability of mulberry to light, temperature, water, soil and other normal conditions objectively empowers it to have numerous environmental defensive capacities in water and soil protection, wind tolerance and sand consolidation, water source preservation, and air refreshment. Since earlier, Ayurveda and other literature have claimed the diverse medicinal properties against certain serious diseases e.g. hypertension, hyperglycemia, cancer, arthritis, diabetes and rheumatis, heart disease, inflammation, fever, cough and throat, stomach related disease, unsteadiness, hepatitis etc. The current studies of different pharmacological compounds of medicinal significance present in mulberry plants opens up new avenues of research in the medical science. Under this study briefly described about economic as well as environmental importance of mulberry taking into account different aspects of its role as animal feed, foodstuff, phytomedicine and remediation of polluted soils and atmosphere, etc. J. Bio-Sci. 29(1): 163-179, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Two Essential Oils From the Asteraceaes Family Against Ectomyelois Ceratoniae Zell. (Lepidoptera, Pyralidae): Case Of Artemisia Herba-Alba Asso. And Artemisia Compestris L.","authors":"L. Ismahane, B. Khaoula, A. Rahim, M. Ibrahim, MM Seghir, H. Ayoub, OM Laid","doi":"10.3329/jbs.v29i2.54950","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54950","url":null,"abstract":"The current work was done Artemisia herba-alba and Artemisia compestris essential oils harvested from the Eastern Algerian Sahara, their insecticidal characteristics against the eggs and adults of the date moth Ectomyelois ceratoniae. Indeed, two treatment modes were used; by contact application on eggs and by inhalation against adults. It appears from the results that the hatch rates were less than the hatching rate recorded in the control (96% ±00.00). The hatching rate reported on eggs treated by the highest dose (160 μl/ml) of Artemisia herba-alba and Artemisia compestris essential oils are 16.66 ± 08.81 and 37.77 ± 13.47 respectively. Statistical treatment results by the Chi-square test (χ2), attest that the treatment by A. herba-alba and Artemisia compestris essential oils at the same dose (160 μl/ml) affect significantly (χ2 = 35.62, p = 0.00 and χ2 = 21.17, p = 0.00 respectively) the hatching rate compared to the control. The sensitivity of adults to essential oils is expressed by 100% mortality rates obtained after 10 min of treatment by the highest doses (80 μl/ml and 160 μl/ml) of A. herba-alba essential oils, the same mortality rates (100%) were notified with the same doses (80 μl/ml and 160 μl/ml) after 20 min and 15 min of treatment by Artemisia compestris essential oils respectively. The dose-dependent mortality data revealed that there was a significant difference between the five doses of A. herba-alba essential oil tested except at the last treatment time (20 min) for which it was appeared p = 0.571, while for A. compestris essential oil, a significant difference was recorded with p varying between 0.00 and 0.003. The lowest LD50 value (0.09 μl/ml and 16.71 μl/ml) were noted during the longest treatment time (20 min), while the highest LD50 value (75.85 μl/ml and 263.7 μl/ml) were found during the shortest time (5 min) of A. herba-alba and A. compestris respectively. J. Bio-Sci. 29(2): 09-17, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Lesions In Goat Brain Collected From Abattoirs in Rajshahi Metropolitan Area","authors":"A. Akta, R. Khatun, MN Khatun, R. Parvin, N. Khatun, Sma Rauf, HM Golbar","doi":"10.3329/jbs.v29i0.54821","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54821","url":null,"abstract":"Brain lesions are important cause of morbidity and reduced productivity in goats worldwide. Works on brain lesion in goats are extremely rare in Bangladesh and remain to be investigated. We investigated lesions in goat brains collected from abattoirs in Rajshahi Metropolitan area by gross and microscopic examination. Randomly selected 57 goat brains were investigated of which 24.56% brains showed gross and histopathologic lesions. Prevalence of brain lesions were higher in Jamnapari goats (29.41%) compared to in Black Bengal goats (22.50%) and female goats (26.47%) were more affected than male goats (21.74%). Age-wise prevalence were 31.25%, 21.43%, 12.50%, and 40.00% in the age of up to 2 years, 2-3 years, 3-4 years, and above 4 years old goats, respectively. Goats were more affected in the rainy (35.29%) season in contrast to in the winter (23.53%) and in the summer (10.52%) seasons. The brains collected from goats with Gid disease showed uni or multilocular cysts containing clear fluid and multiple scolices, the rest of the brains were apparently healthy by macroscopic examination. Histopathologically, pyogranuloma, perivascular cuffing, gliosis, neuronal degeneration, hemorrhages, congestion and thrombus were evident. J. Bio-Sci. 29(1): 49-58, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The UnderstAnding of Enigmas Related to Cancer: Cancer Origin and Progression- A Review","authors":"M. Hasan","doi":"10.3329/jbs.v29i0.54831","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54831","url":null,"abstract":"Human body is an excellent example of self-synchronized system. This coordination is existing between organs, cells, subcellular organels even in molecules by which these are formed and maintaining a subtle pattern of morphogenesis. Although having same responsible genes for metamorphosis of different organisms, the expression pattern of those genes varies in every species. Frequent repetition of a pathway energizes the pathway to be established itself as an ordinary and stable event in that developmental procedure. Cancer can be called a disease of geometry which is also metastasize in human body maintain a developmental pathway. The paradoxes in cancer cannot be explained with the conventional mechanistic approaches; therefore, it requires a holistic approach (Morphogenetic Field) for better understanding. Field effects of various epigenetic factors such as heavy metals and radiations affect the normal developmental pathway of organogenesis and energize the progression of cancer. Quantum field along with gravitational field also interfere with the self-organizing process of a biosystem and have influence on the mutation of different protooncogenes. Understanding of this field concept is very promising for achieving advancement in the various emerging fields of biology like optogenetics, bioinformatics, computational biology and cybernetics etc. In true sense, the morphogenetic field concept offers an exciting new canvas like checkpoint therapy in cancer prognosis. J. Bio-Sci. 29(1): 153-161, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study on Peripheral Neuropathy and Its Related Risk Factors Associated With hba1c Levels","authors":"Mahbub Hossain, Mk Sarkar, I. Mahbub, S. Islam","doi":"10.3329/jbs.v29i2.54961","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54961","url":null,"abstract":"Diabetic peripheral neuropathy (DPN) patients frequently feel persistent pain, which is described as painful diabetic peripheral neuropathy (PDPN), which begins in both feet and frequently spreads to the calves, fingers, and hands. PDPN not only causes pain, but also affects patients' sleep, emotions, mental state, and everyday activities, resulting in a low quality of life and a significant financial burden. The goal of this study was to monitor if there was a link between the prevalence, pattern, and related risk factors of diabetic peripheral neuropathy and hemoglobin A1C (HbA1c) levels. In this crosssectional study, 150 type-2 diabetic patients were screened for DPN with PDPN and their HbA1c level was measured in every three months. DPN, PDPN and non-painful DPN were confirmed in patients displaying both clinical manifestations of neuropathy and neurological abnormalities assessment. DPN was detected in 24% (n = 36), while PDPN was found at 15% (n = 23) of the total patients. The prevalence of PDPN is 63.88% (n = 23) and non-painful DPN is 36.11% (n = 13) of total DPN (n = 36). Out of total PDPN (n = 23), the prevalence of symmetrical pain is 65% (n = 15), asymmetrical 35% (n = 8), sensory 26% (n = 6), motor 13% (n = 3), mixed (sensorimotor) 61% (n = 14), lower limb involvement 48% (n = 11), upper limb13% (n = 3) and both limb 39% (n = 9). In comparison to patients without DPN, both PDPN and non-painful DPN, patients had greater HbA1c levels (p<0.05). Furthermore, advanced age and longer diabetes duration were considerable and significant (p<0.05) risk factors for DPN with PDPN and non-painful DPN respectively. Overall, the findings imply that elevated HbA1c levels are closely linked to DPN, PDPN and non-painful DPN in type-2 diabetic patients and that HbA1c might be used as a predictive marker for DPN with PDPN and non-painful DPN in the patients studied. J. Bio-Sci. 29(2): 123-138, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Ready-to-Cook (Rtc) Hilsa (Tenualosa Ilisha) Curry Under Vacuum and Modified Atmosphere Packaging During Refrigerated Storage","authors":"M.T. Islam, R. Sultana, M. Rahim, M. Hasan, F. Flowra, M. Bashar, Y. Mahmud","doi":"10.3329/jbs.v29i2.54956","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54956","url":null,"abstract":"The aim of the study was to evaluate quality of ready-to-cook (RTC) hilsa curry under not sealed pack as control, vacuum as T1, MAP-1 (50% CO2 & 50% N2) as T2, and MAP-2 (40% CO2 , 30 N2 & 30% O2) pack as T3 during storage at 4±1°C. For this purpose, pH, total volatile base nitrogen (TVB-N), thiobarbituric acid reactive substances (TBARS) and aerobic plate count (APC) of three samples from each treatment were analyzed at four days interval during 28 days of storage. The pH and TVB-N values of RTC hilsa curry were within the standard limit in all samples during the storage period. However, significantly (p <0.05) lower values were observed on and after the 12th day for pH and 16th day for TVBN in all samples compared to the control. TBARS gradually increased from the 4th day for all samples except vacuum packaged sample. However, significantly (p <0.05) lower TBARS were observed in the vacuum and MAP-1 samples on and after the 8th day of storage compared to the control and MAP-2 samples. APCs gradually increased from the initial value of 5.25 log CFU/g with time in all samples. However, significantly (p <0.05) lower APCs were observed on and after the 16th day of storage in all samples compared to the control sample. The APCs exceeded the 7 log CFU/g, which is considered as the upper acceptable limit on approximately 16th day for the control, 24th day for vacuum, 22nd day for MAP-1, and 20th day for MAP-2 sample. Therefore, the vacuum packaging demonstrated the better results, which the superstores can utilize conveniently to display RTC hilsa curry with prolonged shelf life. J. Bio-Sci. 29(2): 71-79, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Protease on Wheat Bran by a Newly Isolated Streptomyces SP. Under Solid State Fermentation","authors":"F. Boughachiche, K. Rachedi, H. Zerizer, R. Duran, S. Karama, M. Biaci, Z. Aknouche, S. Bouchina, A. Boulahrouf","doi":"10.3329/jbs.v29i0.54820","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54820","url":null,"abstract":"Proteases are a group of enzymes that catalyse the degradation of proteins resulting in the production of their amino acid constituents. In the present study newly isolated Streptomyces sp. was subjected to produce proteases through solid state fermentation while wheat bran was used as substrate. To produce proteases, a local strain Streptomyces sp. was isolated from a soil sample of Ezzemouls saltpan, located in Ain M'lila (East of Algeria). The phenotypic and phylogenetic studies of this strain showed that it represents probably a new species. The SSF production medium for Streptomyces sp. was optimized using Plackett and Burman statistical methods. The results showed a maximum activity on basal wheat bran medium supplemented with 1% fructose. The best SSF humidifying solution was pH 9.0 phosphate buffer at 50% moisture. Protease has an optimum at pH 7.0, which is a typical characteristic of neutral proteases. The optimum temperature was 60°C and proved stable up to 80°C. The results showed that the novel Streptomyces sp. isolate is a good producer of extracellular neutral protease on wheat bran, which can be beneficial for industries. J. Bio-Sci. 29(1): 33-48, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pattern of Coronary Heart Disease Among the Admitted Patients In Cardiology Department and Itsrelation to Their Socio-Demographic Characteristics","authors":"A. Awal, J. Sazzad, N. Banu, J. Haque, M. Kamruzzaman","doi":"10.3329/jbs.v29i0.54822","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54822","url":null,"abstract":"Coronary heart disease is a group of conditions affecting the functioning of the heart or blood vessels and is one of the leading causes of death globally. Like other countries, coronary heart disease prevalence is also rising among the adults in Bangladesh. Besides conventional risk factors for coronary heart disease, genetic predisposition and some novel issues like high salt intake, arsenicosis, hypovitaminosis D and air pollution may play important role in the aetiopathogenesis of coronary heart disease in this population. Formulation of appropriate policy and more emphasis on preventive strategy may help combat coronary heart disease in Bangladesh. The main aim of this study was to find out the pattern of coronary heart disease among the patients admitted in Cardiology department of Rajshahi Medical college Hospital and to relate their socio-demographic and economic characteristics with the disease. It was a cross sectional type of descriptive study during February 2019 to July 2019. A total of 352 patients of Cardiology department considered as sample who admitted for their heart problem during the study period selected by purposive sampling. Majorities (85.8%) of the respondents were >40 years among them 74.4% were male. Most (52.0%) of the respondents were from nuclear family and smokers (65.62%). They had history of diabetes mellitus (22.4%) and lead sedentary life (11.4%). Regarding coronary heart disease 74.7% had ischemic heart disease and right coronary artery disease (66.7%). About 42.1% were hypertensive patients and 94.0% took medical treatment. Type of heart disease was found significantly associated with age group of the respondents (p<0.01), smoking habit (p<0.05) and history of abuse substances currently (p<0.05). High coronary heart disease prevalence along with an upward trend was observed in Bangladeshi adults and older age. Proper strategies are required for primary prevention of coronary heart disease so that a further increase can be alleviated and the morbidity and mortality associated with it can be reduced. J. Bio-Sci. 29(1): 59-68, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Mercury Concentration in Water, Bottom Sediment And Two Mollusc Species (Crassostrea Gasar and Tympanotonus Fuscatus) From a Lagos Creek in Nigeria","authors":"AO Lawal Are, R. Moruf, UJ Sobara, KB Salami","doi":"10.3329/jbs.v29i0.54830","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54830","url":null,"abstract":"Concentration of mercury was investigated in the flesh and shell of two species of benthic mollusc, Crassostrea gasar and Tympanotonus fuscatus and in water and sediment from Makoko Creek, adjacent to the Lagos Lagoon between January to September 2019. Values obtained for physicochemical parameters in Makoko Creek (water temperature- 28.92±0.1°C; pH- 7.73±0.02; salinity- 14.23±0.05 ppt; dissolved oxygen- 5.34±0.02 mg/l; biological oxygen demand- 7.780±0.1 mg/l and chemical oxygen demand- 12.34±0.02 mg/l) were within the acceptable levels for survival, metabolism and physiology of aquatic organism. The concentration of mercury followed decreasing order as sediment >water >flesh >shell across locations for both species. For all the tested samples of C. gasar and T. fuscatus, biowater accumulation factor in flesh and shell were higher than those of bio-sediment accumulation factor. The coefficients of variance (CV %) in shells were lower than those of the flesh for both investigated mollusc species. It was shown that mercury contents of flesh or shells of C. gasar and T. fuscatu are directly affected by those of water and bottom sediment. J. Bio-Sci. 29(1): 143-151, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testosterone Therapy Could Be a Potential Approach For Treatment of Elderly Male COVID-19 Patients- A Review","authors":"M. Gofur","doi":"10.3329/jbs.v29i0.54833","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54833","url":null,"abstract":"The emerging SARS-CoV-2 (2019-nCoV) is still following the increasing trend of infection worldwide with an elderly male predominance. Studies reported that majority of male patients with COVID-19 suffer from severe testosterone deficiency and are likely to die from the coronavirus mainly due to a less effective immune response and high level of pro-inflammatory cytokines, a condition called cytokine storm. Testosterone (a steroid having protective anti-inflammatory properties) therapy reduces circulating levels of pro-inflammatory cytokines. Testosterone therapy is effective in the management of elderly Alzheimer's disease where pro-inflammatory cytokines are the potent mediators of inflammatory processes, and aged men having HIV infection. As, currently there is no specific therapeutic drug targeting the SARS-CoV-2, and, considering the present pandemic circumstance, and the immunomodulatory and protective findings of testosterone therapy in previous studies, it can be hypothesized that testosterone therapy could be a potential approach for treatment of elderly male COVID-19 patients. J. Bio-Sci. 29(1): 181-187, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}