Bone Reports最新文献

{"title":"“Cellularity as a predictive tool for mesenchymal stem cell concentration in bone marrow concentrates: Implications for regenerative medicine”","authors":"Klaus Werner Labarre,&nbsp;Peter Ansgar Grathwol,&nbsp;Gerald Zimmermann","doi":"10.1016/j.bonr.2024.101820","DOIUrl":"10.1016/j.bonr.2024.101820","url":null,"abstract":"<div><h3>Background</h3><div>Mesenchymal stem cells (MSCs) derived from bone marrow play an increasingly important role in regenerative medicine due to their capacity to promote tissue regeneration in various clinical contexts. Applications include the treatment of osteoarthritis, bone regeneration post-injury, and the management of conditions such as Crohn's disease, alopecia, and nervous system reconstruction. Accurate quantification of MSCs within Bone Marrow Concentrates (BMCs) is essential for ensuring the quality and efficacy of these cell therapy products in clinical settings.</div></div><div><h3>Objective</h3><div>This study aims to quantify the population of CD271⁺ and CD45⁻ cells in BMCs prepared using the method we have selected and to provide a basis for comparing these results with other BMC products. Additionally, we seek to determine whether the total cell count in BMCs can serve as a reliable indicator of MSC numbers and if cellularity (the number of cells per ml) can predict a higher percentage of MSCs within the population.</div></div><div><h3>Methods</h3><div>Bone Marrow Aspirates (BMA) were collected from 41 patients undergoing knee or hip arthroplasty. Aspirates were processed using density gradient centrifugation and positive selection of CD271⁺ cells. Flow cytometry was applied to analyze cell subsets, and cell counts were determined with a NucleoCounter. The relationships between BMA cellularity (total cells per ml), MSC concentration (MSC count per ml), and MSC percentage (the proportion of MSCs within the total cell population) were assessed.</div></div><div><h3>Results</h3><div>The mean percentage of CD271⁺ CD45⁻ cells in bone marrow samples was 0.03 % (SD 0.03 %). Cellularity varied significantly among samples, with a mean of 6 million cells/ml (SD 8.7 million cells/ml). A strong correlation was observed between BMC cellularity and MSC concentration (<em>p</em> &lt; 0.05), although no correlation was found between cellularity and the MSC percentage.</div></div><div><h3>Conclusion</h3><div>Despite high variability in cellularity, the concentration of MSCs correlated strongly with BMC cellularity, suggesting that total cell counts can be used to estimate MSC numbers in BMCs. However, cellularity is not an indicator of a particularly high MSC content. This study supports the use of cell counts as a measure for estimating MSC concentration in BMCs. Future research should focus on establishing direct comparisons with other BMC products and exploring factors influencing cellularity and MSC percentages to enhance BMC quality for clinical applications.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"24 ","pages":"Article 101820"},"PeriodicalIF":2.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of stopping burosumab treatment at the end of skeletal growth in adolescents with X-linked hypophosphatemia (XLH)","authors":"Charlotte Jarvis ,&nbsp;Renuka Ramakrishnan ,&nbsp;Poonam Dharmaraj ,&nbsp;Talat Mushtaq ,&nbsp;Sanjay Gupta ,&nbsp;Angela Williams ,&nbsp;Angela J. Rylands ,&nbsp;Helen Barham ,&nbsp;Annabel Nixon ,&nbsp;Suma Uday","doi":"10.1016/j.bonr.2024.101819","DOIUrl":"10.1016/j.bonr.2024.101819","url":null,"abstract":"<div><div>Many adolescents with X-linked hypophosphatemia (XLH) currently have to stop treatment with burosumab at the end of skeletal growth. We describe the experience of a cohort of adolescents with XLH before, during, and after stopping burosumab (median treatment duration 37.5 months). Improvements in serum phosphate, pain, mobility, function, and quality of life noted during burosumab treatment were reversed after treatment cessation. Further real-world data are needed to explore the value of uninterrupted burosumab treatment in adolescents.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"24 ","pages":"Article 101819"},"PeriodicalIF":2.1,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring shape changes in healthy bone growth through 3D spatiotemporal statistical shape models: A scoping review","authors":"Lily E. de Vries ,&nbsp;Derek F.R. van Loon ,&nbsp;Eline M. van Es ,&nbsp;DirkJan H.E.J. Veeger ,&nbsp;Joost W. Colaris","doi":"10.1016/j.bonr.2024.101817","DOIUrl":"10.1016/j.bonr.2024.101817","url":null,"abstract":"<div><h3>Objective</h3><div>Analyzing population trends of bone shape variation can provide valuable insights into growth processes. This review aims to overview state-of-the-art spatiotemporal statistical shape modeling techniques, emphasizing their application to 3D skeletal structures during healthy growth.</div></div><div><h3>Methods</h3><div>We searched PubMed and Scopus for articles on statistical shape modeling using a pediatric spatiotemporal dataset of 3D healthy bone models. Dataset characteristics and details on the shape models' development, analyses, and potential clinical use were extracted.</div></div><div><h3>Results</h3><div>Fourteen studies were found eligible, modeling one or multiple lower limb bones, the mandible, the skull, and vertebrae. The majority applied Principal Component Analysis on point distribution models to create a statistical shape model. Shape variation was analyzed based on shape modes, representing a specific shape change as a part of the overall variance. Unscaled models resulted in a more compact statistical shape model than scaled models. The latter represented more subtle shape variations due to the absence of size differences between the bone models. Four studies reported a significant correlation between the first shape mode and age, indicating a relationship between that type of shape variation and growth. Three studies reconstructed 3D models using prediction features of statistical shape modeling. Measuring difference between predicted and actual anatomy resulted in Root Mean Squared Errors below 3 mm.</div></div><div><h3>Conclusion</h3><div>Spatiotemporal statistical shape modeling provides insight into modes of shape variation during growth. Such a model can be used to find predictive factors, like age or sex, and deploy these characteristics to predict someone's bone geometry.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"24 ","pages":"Article 101817"},"PeriodicalIF":2.1,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells","authors":"Masakazu Okamoto ,&nbsp;Yusuke Inagaki ,&nbsp;Kensuke Okamura ,&nbsp;Yoshinobu Uchihara ,&nbsp;Kenichiro Saito ,&nbsp;Akihito Kawai ,&nbsp;Munehiro Ogawa ,&nbsp;Akira Kido ,&nbsp;Eiichiro Mori ,&nbsp;Yasuhito Tanaka","doi":"10.1016/j.bonr.2024.101816","DOIUrl":"10.1016/j.bonr.2024.101816","url":null,"abstract":"<div><div>Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is an essential tool for gene expression analysis; choosing appropriate reference genes for normalization is crucial to ensure data reliability. However, most studies on osteogenic differentiation have had limited success in identifying optimal reference genes. To the best of our knowledge, no optimal reference genes in three-dimensional (3D) osteogenic differentiation culture experiments using human induced pluripotent stem cells (hiPSCs) have been identified. In this study, we aimed to identify stable reference genes that could be used for normalization in gene expression analyses during the 3D osteogenic differentiation of hiPSCs using an atelocollagen sponge as a scaffold. Four algorithms—ΔCt, BestKeeper, NormFinder, and geNorm—were used to evaluate the stability of 14 candidate reference genes. Genes encoding TATA box-binding protein, hypoxanthine phosphoribosyltransferase 1, and 14–3-3 protein zeta polypeptide were identified as the most stable reference genes. In comparison, conventionally used reference genes (beta-2 microglobulin and beta-actin genes) ranked among those with low stability. We also demonstrated the successful 3D osteogenic differentiation of hiPSCs on atelocollagen sponge. Our findings provide valuable insights for reference gene selection and bone tissue regeneration from hiPSCs, which could improve the treatment prospects for bone defects and other similar conditions in regenerative medicine.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"23 ","pages":"Article 101816"},"PeriodicalIF":2.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringin promotes osteogenic potential in bone marrow-derived mesenchymal stem cells via mediation of miR-26a/Ski axis","authors":"Jiawei Zou,&nbsp;Longze Zhou,&nbsp;Guoqiang Liu,&nbsp;Ying Zhang,&nbsp;Lingguo Zeng","doi":"10.1016/j.bonr.2024.101815","DOIUrl":"10.1016/j.bonr.2024.101815","url":null,"abstract":"<div><h3>Background</h3><div>Osteonecrosis of the femoral head (ONFH) is a common orthopedic disease, which seriously affects the quality of life of patients. Naringin has protective effect on ONFH. In present study, we aimed to investigate the mechanism of Naringin regulating miR-26a in ONFH.</div></div><div><h3>Methods</h3><div>Two sequencing datasets (GSE89587 for micro-RNA, GSE123568 for mRNA) related to ONFH were obtained from the GEO database for bioinformatics analysis. Bone marrow-derived mesenchymal stem cells (BMSCs) were treated with adipogenic medium (AM) or osteogenic medium (OM), and then treated with 10 μM, 100 μM or 1000 μM Naringin. Gene and protein levels were detected by RT-qPCR and Western blotting. ALP activity and alizarin red staining (ARS) were applied to investigate the osteogenic differentiation of BMSCs. Oil red O staining was performed to test adipogenic differentiation. The content of triglycerides (TG) in BMSCs was detected by TG detection kit. Double luciferase reporter gene measured the interaction between miR-26a and Ski.</div></div><div><h3>Results</h3><div>Bioinfomatic analyses indicated a significant downregulation of miR-26a and a significant upregulation of Ski in the peripheral blood of patients with ONFH. Naringin significantly promoted the osteogenic differentiation, and increased the ALP activity and ARS. Meanwhile, it decreased the adipogenic differentiation and inhibited TG levels. In addition, miR-26a was downregulated and Ski was increased in AM-treated BMSCs, while miR-26a was upregulated and Ski was decreased in OM-treated BMSCs. Furthermore, miR-26a promoted the osteogenic differentiation and suppressed the adipogenic differentiation in BMSCs. Moreover, Naringin enhanced osteogenic potential in BMSCs was reversed by knockdown of miR-26a or overexpression of Ski.</div></div><div><h3>Conclusion</h3><div>Naringin could promote osteogenic differentiation of BMSCs via mediation of miR-26a/Ski axis. Thereby, Naringin might be a new agent for ONFH treatment.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"23 ","pages":"Article 101815"},"PeriodicalIF":2.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvements with burosumab treatment in an early access programme for adults with X-linked hypophosphataemia: A case series of three patients","authors":"Julia Day ,&nbsp;Chandrin Jayatilleke ,&nbsp;Matthew Roy","doi":"10.1016/j.bonr.2024.101814","DOIUrl":"10.1016/j.bonr.2024.101814","url":null,"abstract":"<div><div>X-linked hypophosphataemia (XLH) is a life-long phosphate-wasting disorder that causes skeletal deformities, pain, stiffness, and fatigue and impairs quality of life. Burosumab was approved for use in adults in 2020. We describe three adults with persistent XLH symptoms who received burosumab treatment in a real-world setting. Patients report improvements in pain, mobility, physical function, energy, fatigue, and mental wellbeing through patient-reported outcome measures, enriched with further detail from written testimonials.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"23 ","pages":"Article 101814"},"PeriodicalIF":2.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of daily physical activity and bone microarchitecture in young adults with type 1 diabetes — A pilot exploratory study","authors":"Sarah L. West ,&nbsp;Michelle Furman ,&nbsp;Rahim Moineddin ,&nbsp;Etienne Sochett","doi":"10.1016/j.bonr.2024.101813","DOIUrl":"10.1016/j.bonr.2024.101813","url":null,"abstract":"<div><h3>Purpose</h3><div>Physical activity (PA) is an important determinant of skeletal health. In young adults with type 1 diabetes (T1D) fracture risk is increased, yet few studies have examined the PA and bone health relationship. Therefore, this pilot cross-sectional study characterized PA levels and their association with bone parameters measured by high resolution peripheral quantitative computed tomography (HR-pQCT) in young adults with T1D.</div></div><div><h3>Methods</h3><div>HR-pQCT (Xtreme CTII) was used to measure bone outcomes at the distal tibia and radius, and accelerometery (ActiGraph GT3X) recorded daily minutes of light and moderate-vigorous physical activity (MVPA). Quadratic regression analyses were conducted with a <em>p</em>-value ≤ 0.05 considered significant.</div></div><div><h3>Results</h3><div>PA data from 19 young adults (23.1 ± 1.9 years) with T1D was analyzed. Over half (63 %) of participants completed ≥150 min of MVPA per week, however, most measured activity time per day (57 %) was spent in sedentary pursuits. Significant non-linear associations were found between the duration of MVPA and several trabecular bone parameters at the tibia.</div></div><div><h3>Conclusions</h3><div>In young adults with T1D, MVPA may have site specific (tibia) and compartment specific (trabecular) non-linear associations with bone. Further studies should confirm these findings, which may help inform evidence-based exercise recommendations to optimize bone health in young adults with T1D.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"23 ","pages":"Article 101813"},"PeriodicalIF":2.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoledronate interrupts pre-osteoclast-induced angiogenesis via SDF-1/CXCR4 pathway","authors":"Mohamed Awad ,&nbsp;Elizabeth Taylor-Diaz ,&nbsp;Amany Tawfik ,&nbsp;Khaled Hussein ,&nbsp;Ahmed Elmansi ,&nbsp;Mahmoud Elashiry ,&nbsp;Ranya Elsayed ,&nbsp;Linah Shahoumi ,&nbsp;James Borke ,&nbsp;William Hill ,&nbsp;Fanglong Dong ,&nbsp;Mohammed E. Elsalanty","doi":"10.1016/j.bonr.2024.101812","DOIUrl":"10.1016/j.bonr.2024.101812","url":null,"abstract":"<div><h3>Introduction</h3><div>In this study, we tested the hypothesis that pre-osteoclast signaling is key in triggering post-traumatic angiogenesis in alveolar bone via the SDF-1/CXCR4 pathway. Interruption of osteoclast differentiation through zoledronate (Zol) disrupts the crosstalk between pre-osteoclasts and endothelial cells, hindering the initial angiogenic reaction following dental trauma. This disruption could therefore play a role in the pathogenesis of medication-related osteonecrosis of the jaw (MRONJ).</div></div><div><h3>Methods</h3><div>The effect of zoledronate on the expression of SDF1 was tested in pre-osteoclasts (POC) in vitro. Then, we tested the effect of pre-osteoclast conditioned medium on HUVEC cell differentiation, migration, tube-formation, and CXCR4 expression and activity in-vitro. Lastly, we quantified the effect of zoledronate treatment on post-traumatic vascular perfusion of alveolar bone, using microCT-angiography and immunohistochemistry.</div></div><div><h3>Results</h3><div>SDF-1 mRNA expression decreased in Zol-treated POCs (<em>p</em> = 0.02). Flow-Cytometry analysis showed a decrease in CXCL-12⁺ (SDF-1α) expressing POCs with Zol treatment (<em>p</em> = 0.0058). On the other hand, CXCR4 mRNA expression was significantly inhibited in Zol-treated HUVECs (<em>p</em> = 0.0063). CXCR4 protein expression and activity showed a corresponding dose-dependent downregulation HUVEC surface treated with conditioned media from POC treated with Zol (<em>p</em> = 0.008 and 0.03, respectively). Similar inhibition was observed of HUVEC migration (<em>p</em> = 0.0012), and tube formation (<em>p</em> &lt; 0.0001), effects that were reversed with SDF-1. Finally, there was a significant reduction of CD31⁺ HUVECs in Alveolar bone of Zol-treated rats (<em>p</em> = 0.0071), confirmed by significantly lower percentage of blood vessel volume (<em>p</em> = 0.026), and marginally lower vessel number (<em>p</em> = 0.062) in the alveolar bone.</div></div><div><h3>Conclusion</h3><div>Pre-osteoclasts play a crucial role in the initial angiogenic response in alveolar bone following dental extraction. Disruption of this process may be a predisposing factor to osteonecrosis.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"23 ","pages":"Article 101812"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment with bariatric surgery in patients with osteogenesis imperfecta and severe obesity","authors":"Jannie Dahl Hald ,&nbsp;Asta-Marie Welander Hald ,&nbsp;Torben Harsløf ,&nbsp;Bente Langdahl ,&nbsp;Jens Meldgaard Bruun","doi":"10.1016/j.bonr.2024.101811","DOIUrl":"10.1016/j.bonr.2024.101811","url":null,"abstract":"","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"23 ","pages":"Article 101811"},"PeriodicalIF":2.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voluntary exercise in mice triggers an anti-osteogenic and pro-tenogenic response in the ankle joint without affecting long bones","authors":"Anne Briolay ,&nbsp;François Duboeuf ,&nbsp;Séverine Delplace ,&nbsp;Leyre Brizuela ,&nbsp;Olivier Peyruchaud ,&nbsp;David Magne ,&nbsp;Carole Bougault","doi":"10.1016/j.bonr.2024.101810","DOIUrl":"10.1016/j.bonr.2024.101810","url":null,"abstract":"<div><div>Biomechanical stimulation is proposed to occupy a central place in joint homeostasis, but the precise contribution of exercise remains elusive. We aimed to characterize in vivo the impact of mechanical stimulation on the cell-controlled regulation of ossification within the ankles of healthy mice undergoing mild physical activity. DBA/1 male mice were subjected to voluntary running exercise for two weeks, and compared to mice housed in standard conditions (<em>n</em> = 20 per group). Free access to activity wheels resulted in a running exercise of 5.5 ± 0.8 km/day at 14.5 ± 0.5 m/min. Serum levels of alkaline phosphatase, IL-6, IL-8/Kc, IL-17a, and TNF-α were measured. No change in systemic inflammation was detected. The bone architecture of the femur and the calcaneus was unchanged, as revealed by μCT and histology of the enthesis of the Achilles tendon. mRNAs were extracted from femurs, tibias, and ankle joints before RT-qPCR analysis. The expression of the mechanosensitive genes <em>Sclerostin</em> (<em>Sost</em>) and <em>Periostin</em> (<em>Postn</em>) was not impacted by the exercise in long bones. Oppositely, <em>Sost</em> and <em>Postn</em> levels were modulated by exercise in joints, and osteogenic markers (<em>Col10a1</em>, <em>Runx2</em>, <em>Osx</em>, and <em>Dmp1</em>) were downregulated in the exercise group. In addition, the tenogenic markers <em>Scx</em>, <em>Mkx</em>, and <em>Tnmd</em> were upregulated by exercise. Thus, voluntary exercise affected the phenotype of joint cells without impacting long bones. As gene expression of <em>Bmp2</em>, <em>Bmp4</em>, and <em>Id1</em> was also reduced in these cells, an off-regulation of BMP signaling could be partly responsible for their mechanosensitive response. Running exercise seemed to preserve the tendon from its progressive ossification, as seen in numerous enthesopathies. This study paves the way to future experiments for investigating the effects of mechanical stimulation in various mouse models.</div></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"23 ","pages":"Article 101810"},"PeriodicalIF":2.1,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}