Translation (Austin, Tex.)最新文献_第8页

Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG 含荧光帽类似物anthaniloyl - m7gpppg的mrna的高效制备和性质

Translation (Austin, Tex.) Pub Date : 2015-01-02 DOI: 10.4161/21690731.2014.988538

D. Gunawardana, A. Domashevskiy, K. Gayler, D. Goss

引用次数: 9

Eukaryote-specific extensions in ribosomal proteins of the small subunit: Structure and function 核糖体小亚基蛋白的真核生物特异性延伸:结构和功能

Translation (Austin, Tex.) Pub Date : 2015-01-02 DOI: 10.1080/21690731.2014.999576

Arnab Ghosh, A. Komar

引用次数: 5

The role of the poly(A) binding protein in the assembly of the Cap-binding complex during translation initiation in plants 植物翻译起始过程中poly(A)结合蛋白在cap结合复合体组装中的作用

Translation (Austin, Tex.) Pub Date : 2014-12-22 DOI: 10.4161/2169074X.2014.959378

D. Gallie

{"title":"The role of the poly(A) binding protein in the assembly of the Cap-binding complex during translation initiation in plants","authors":"D. Gallie","doi":"10.4161/2169074X.2014.959378","DOIUrl":"https://doi.org/10.4161/2169074X.2014.959378","url":null,"abstract":"Translation initiation in eukaryotes requires the involvement of multiple initiation factors (eIFs) that facilitate the binding of the 40 S ribosomal subunit to an mRNA and assemble the 80 S ribosome at the correct initiation codon. eIF4F, composed of eIF4E, eIF4A, and eIF4G, binds to the 5′-cap structure of an mRNA and prepares an mRNA for recruitment of a 40 S subunit. eIF4B promotes the ATP-dependent RNA helicase activity of eIF4A and eIF4F needed to unwind secondary structure present in a 5′-leader that would otherwise impede scanning of the 40 S subunit during initiation. The poly(A) binding protein (PABP), which binds the poly(A) tail, interacts with eIF4G and eIF4B to promote circularization of an mRNA and stimulates translation by promoting 40 S subunit recruitment. Thus, these factors serve essential functions in the early steps of protein synthesis. Their assembly and function requires multiple interactions that are competitive in nature and determine the nature of interactions between the termini of an mRNA. In this review, the domain organization and partner protein interactions are presented for the factors in plants which share similarities with those in animals and yeast but differ in several important respects. The functional consequences of their interactions on factor activity are also discussed.","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"131 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88355278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

TRANS PROF DB: A new resource for sharing translational profiles. transprofdb：一个共享翻译概要文件的新资源。

Translation (Austin, Tex.) Pub Date : 2014-10-30 eCollection Date: 2014-09-01 DOI: 10.4161/2169074X.2014.965615

Ruth V Spriggs, Anne E Willis

{"title":"TRANS PROF DB: A new resource for sharing translational profiles.","authors":"Ruth V Spriggs, Anne E Willis","doi":"10.4161/2169074X.2014.965615","DOIUrl":"10.4161/2169074X.2014.965615","url":null,"abstract":"The translational efficiency of individual mRNAs can be measured on a genome-wide scale using translational profiling techniques. Data from such experiments are an enormously important resource in the quest to understand the impact of cellular state on gene expression. To improve our understanding of these data, we have created TRANS PROF DB, a manually curated resource containing the translational status of human mRNAs under defined conditions. Results are provided at the level of an annotated conclusion for each gene, e.g. \"Translation up-regulated\", and also, where available, in a rawer form such as the normalized analyzed microarray output. TRANS PROF DB aims to provide a central resource for the sharing of translational profiles to facilitate reuse of published data and to enable meta-analyses across data sets. As the database expands, it will provide an easily searchable archive of publicly available translational profiling data sets. We encourage all researchers to deposit their translational profiling data into TRANS PROF DB to enable us to create a truly comprehensive resource. TRANS PROF DB is available without restriction at mrctools.mrctox.le.ac.uk/TRANS_PROF_DB. ","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"29 1","pages":"e965615"},"PeriodicalIF":0.0,"publicationDate":"2014-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82210813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weighing up the possibilities: Controlling translation by ubiquitylation and sumoylation. 权衡各种可能性：通过泛素化和聚合化控制翻译。

Translation (Austin, Tex.) Pub Date : 2014-10-30 eCollection Date: 2014-09-01 DOI: 10.4161/2169074X.2014.959366

Felicity Z Watts, Robert Baldock, Jirapas Jongjitwimol, Simon J Morley

{"title":"Weighing up the possibilities: Controlling translation by ubiquitylation and sumoylation.","authors":"Felicity Z Watts, Robert Baldock, Jirapas Jongjitwimol, Simon J Morley","doi":"10.4161/2169074X.2014.959366","DOIUrl":"10.4161/2169074X.2014.959366","url":null,"abstract":"Regulation of protein synthesis is of fundamental importance to cells. It has a critical role in the control of gene expression, and consequently cell growth and proliferation. The importance of this control is supported by the fact that protein synthesis is frequently upregulated in tumor cells. The major point at which regulation occurs is the initiation stage. Initiation of translation involves the interaction of several proteins to form the eIF4F complex, the recognition of the mRNA by this complex, and the subsequent recruitment of the 40S ribosomal subunit to the mRNA. This results in the formation of the 48S complex that then scans the mRNA for the start codon, engages the methionyl-tRNA and eventually forms the mature 80S ribosome which is elongation-competent. Formation of the 48S complex is regulated by the availability of individual initiation factors and through specific protein-protein interactions. Both of these events can be regulated by post-translational modification by ubiquitin or Ubls (ubiquitin-like modifiers) such as SUMO or ISG15. We provide here a summary of translation initiation factors that are modified by ubiquitin or Ubls and, where they have been studied in detail, describe the role of these modifications and their effects on regulating protein synthesis. ","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"36 1","pages":"e959366"},"PeriodicalIF":0.0,"publicationDate":"2014-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81414390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AURA 2 将2

Translation (Austin, Tex.) Pub Date : 2014-01-01 DOI: 10.4161/trla.27738

E. Dassi, Angela Re, S. Leo, T. Tebaldi, L. Pasini, D. Peroni, A. Quattrone

{"title":"AURA 2","authors":"E. Dassi, Angela Re, S. Leo, T. Tebaldi, L. Pasini, D. Peroni, A. Quattrone","doi":"10.4161/trla.27738","DOIUrl":"https://doi.org/10.4161/trla.27738","url":null,"abstract":"Post-transcriptional regulation (PTR) of gene expression is now recognized as a major determinant of cell phenotypes. The recent availability of methods to map protein-RNA interactions in entire transcriptomes such as RIP, CLIP and their variants, together with global polysomal and ribosome profiling techniques, are driving the exponential accumulation of vast amounts of data on mRNA contacts in cells, and of corresponding predictions of PTR events. However, this exceptional quantity of information cannot be exploited at its best to reconstruct potential PTR networks, as it still lies scattered throughout several databases and in isolated reports of single interactions. To address this issue, we developed the second and vastly enhanced version of the Atlas of UTR Regulatory Activity (AURA 2), a meta-database centered on mapping interaction of trans-factors with human and mouse UTRs. AURA 2 includes experimentally demonstrated binding sites for RBPs, ncRNAs, thousands of cis-elements, variations, RNA epigenetics data and more. Its user-friendly interface offers various data-mining features including co-regulation search, network generation and regulatory enrichment testing. Gene expression profiles for many tissues and cell lines can be also combined with these analyses to display only the interactions possible in the system under study. AURA 2 aims at becoming a valuable toolbox for PTR studies and at tracing the road for how PTR network-building tools should be designed. AURA 2 is available at http://aura.science.unitn.it.","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74597250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Regulation of translation dynamic and neoplastic conversion by tRNA and their pieces tRNA及其片段对翻译动态和肿瘤转化的调控

Translation (Austin, Tex.) Pub Date : 2014-01-01 DOI: 10.4161/trla.28586

R. Geslain, G. Eriani

引用次数: 6

Induction of cap-independent BiP (hsp-3) and Bcl-2 (ced-9) translation in response to eIF4G (IFG-1) depletion in C. elegans 线虫对eIF4G (IFG-1)耗用诱导帽非依赖性BiP (hsp-3)和Bcl-2 (ced-9)翻译

Translation (Austin, Tex.) Pub Date : 2014-01-01 DOI: 10.4161/trla.28935

J. K. Morrison, Andrew J. Friday, M. Henderson, E. Hao, B. Keiper

{"title":"Induction of cap-independent BiP (hsp-3) and Bcl-2 (ced-9) translation in response to eIF4G (IFG-1) depletion in C. elegans","authors":"J. K. Morrison, Andrew J. Friday, M. Henderson, E. Hao, B. Keiper","doi":"10.4161/trla.28935","DOIUrl":"https://doi.org/10.4161/trla.28935","url":null,"abstract":"During apoptosis, activated caspases cleave the translation initiation factor eIF4G. This cleavage disrupts cap-dependent mRNA translation initiation within the cell. However, a specific subset of mRNAs can still be recruited for protein synthesis in a cap-independent manner by the residual initiation machinery. Many of these mRNAs, including cell death related mRNAs, contain internal ribosome entry sites (IRESes) that promote their enhanced translation during apoptosis. Still other mRNAs have little dependence on the cap recognition mechanism. The expression of the encoded proteins, both anti- and pro-apoptotic, allows for an initial period of attempted cell survival, then commitment to cell death when damage is extensive. In this study we address the translational regulation of the stress and apoptosis-related mRNAs in C. elegans: BiP (hsp-3) (hsp-4), Hif-1 (hif-1), p53 (cep-1), Bcl-2 (ced-9) and Apaf-1 (ced-4). Altered translational efficiency of these messages was observed upon depletion of cap-dependent translation and induction of apoptosis within the C. elegans gonad. Our findings suggest a physiological link between the cap-independent mechanism and the enhanced translation of hsp-3 and ced-9. This increase in the efficiency of translation may be integral to the stress response during the induction of physiological apoptosis.","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86576018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Signaling crosstalk between the mTOR complexes mTOR复合物之间的信号串扰

Translation (Austin, Tex.) Pub Date : 2014-01-01 DOI: 10.4161/trla.28174

Jianling Xie, C. Proud

引用次数: 52

Why is start codon selection so precise in eukaryotes? 为什么真核生物的起始密码子选择如此精确?

Translation (Austin, Tex.) Pub Date : 2014-01-01 DOI: 10.4161/trla.28387

K. Asano

引用次数: 64