Translation (Austin, Tex.)最新文献_第7页

Corrigendum. 勘误表。

Translation (Austin, Tex.) Pub Date : 2016-04-27 eCollection Date: 2016-01-01 DOI: 10.1080/21690731.2016.1179079

引用次数: 0

tRNA wobble modifications and protein homeostasis. tRNA摆动修饰与蛋白质稳态。

Translation (Austin, Tex.) Pub Date : 2016-01-28 eCollection Date: 2016-01-01 DOI: 10.1080/21690731.2016.1143076

Namit Ranjan, Marina V Rodnina

引用次数: 63

Characterizing inactive ribosomes in translational profiling. 翻译分析中非活性核糖体的特征。

Translation (Austin, Tex.) Pub Date : 2016-01-08 eCollection Date: 2016-01-01 DOI: 10.1080/21690731.2015.1138018

Botao Liu, Shu-Bing Qian

{"title":"Characterizing inactive ribosomes in translational profiling.","authors":"Botao Liu, Shu-Bing Qian","doi":"10.1080/21690731.2015.1138018","DOIUrl":"https://doi.org/10.1080/21690731.2015.1138018","url":null,"abstract":"<p><p>The broad impact of translational regulation has emerged explosively in the last few years in part due to the technological advance in genome-wide interrogation of gene expression. During mRNA translation, the majority of actively translating ribosomes exist as polysomes in cells with multiple ribosomes loaded on a single transcript. The importance of the monosome, however, has been less appreciated in translational profiling analysis. Here we report that the monosome fraction isolated by sucrose sedimentation contains a large quantity of inactive ribosomes that do not engage on mRNAs to direct translation. We found that the elongation factor eEF2, but not eEF1A, stably resides in these non-translating ribosomes. This unique feature permits direct evaluation of ribosome status under various stress conditions and in the presence of translation inhibitors. Ribosome profiling reveals that the monosome has a similar but not identical pattern of ribosome footprints compared to the polysome. We show that the association of free ribosomal subunits minimally contributes to ribosome occupancy outside of the coding region. Our results not only offer a quantitative method to monitor ribosome availability, but also uncover additional layers of ribosome status needed to be considered in translational profiling analysis. </p>","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"4 1","pages":"e1138018"},"PeriodicalIF":0.0,"publicationDate":"2016-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21690731.2015.1138018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34604835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Hippuristanol - A potent steroid inhibitor of eukaryotic initiation factor 4A. hipuristol -真核起始因子4A的有效类固醇抑制剂。

Translation (Austin, Tex.) Pub Date : 2016-01-04 eCollection Date: 2016-01-01 DOI: 10.1080/21690731.2015.1137381

Regina Cencic, Jerry Pelletier

引用次数: 45

Ribosomal protein uS19 mutants reveal its role in coordinating ribosome structure and function 核糖体蛋白uS19突变揭示其在协调核糖体结构和功能中的作用

Translation (Austin, Tex.) Pub Date : 2015-07-03 DOI: 10.1080/21690731.2015.1117703

A. M. Bowen, S. Musalgaonkar, Christine A. Moomau, Suna P. Gulay, Mary Mirvis, J. Dinman

{"title":"Ribosomal protein uS19 mutants reveal its role in coordinating ribosome structure and function","authors":"A. M. Bowen, S. Musalgaonkar, Christine A. Moomau, Suna P. Gulay, Mary Mirvis, J. Dinman","doi":"10.1080/21690731.2015.1117703","DOIUrl":"https://doi.org/10.1080/21690731.2015.1117703","url":null,"abstract":"Prior studies identified allosteric information pathways connecting functional centers in the large ribosomal subunit to the decoding center in the small subunit through the B1a and B1b/c intersubunit bridges in yeast. In prokaryotes a single SSU protein, uS13, partners with H38 (the A-site finger) and uL5 to form the B1a and B1b/c bridges respectively. In eukaryotes, the SSU component was split into 2 separate proteins during the course of evolution. One, also known as uS13, participates in B1b/c bridge with uL5 in eukaryotes. The other, called uS19 is the SSU partner in the B1a bridge with H38. Here, polyalanine mutants of uS19 involved in the uS19/uS13 and the uS19/H38 interfaces were used to elucidate the important amino acid residues involved in these intersubunit communication pathways. Two key clusters of amino acids were identified: one located at the junction between uS19 and uS13, and a second that appears to interact with the distal tip of H38. Biochemical analyses reveal that these mutations shift the ribosomal rotational equilibrium toward the unrotated state, increasing ribosomal affinity for tRNAs in the P-site and for ternary complex in the A-site, and inhibit binding of the translocase, eEF2. These defects in turn affect specific aspects of translational fidelity. These findings suggest that uS19 plays a critical role as a conduit of information exchange between the large and small ribosomal subunits directly through the B1a, and indirectly through the B1b/c bridges.","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81519077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Introducing a class of standardized and interchangeable parts utilizing programmed ribosomal frameshifts for synthetic biology applications 引入一类标准化和可互换的部分利用程序核糖体帧移位合成生物学的应用

Translation (Austin, Tex.) Pub Date : 2015-07-03 DOI: 10.1080/21690731.2015.1112458

Harland E Brandon, Jenna Friedt, Graeme D Glaister, Suneet K Kharey, Dustin D. Smith, Zak K Stinson, Hans-Joachim Wieden

{"title":"Introducing a class of standardized and interchangeable parts utilizing programmed ribosomal frameshifts for synthetic biology applications","authors":"Harland E Brandon, Jenna Friedt, Graeme D Glaister, Suneet K Kharey, Dustin D. Smith, Zak K Stinson, Hans-Joachim Wieden","doi":"10.1080/21690731.2015.1112458","DOIUrl":"https://doi.org/10.1080/21690731.2015.1112458","url":null,"abstract":"Synthetic biology and the rational design of biological devices depend on the availability of standardized and interchangeable biological parts with diverse range of functions. Reliable access to different reading frames during translation has largely been overlooked as functionality for bioengineering applications. Here we report the construction and initial characterization of the first member of such a class of biological parts that conforms to the BioBrick Standard (RFC25), allowing its interchangeable use in biological devices. Using our standardized frameshifting signal consisting of a UUUAAAG slippery sequence, a 6 nt spacer and an engineered pseudoknot based on the infectious bronchitis virus pseudoknot PK401 embedded in a dual reporter construct, we confirm that the frameshifting activity is comparable to the previously published frequency despite the introduced sequence changes. The frameshifting activity is demonstrated using SDS-PAGE and fluorescence spectroscopy. Standardized programmable ribosomal frameshift parts with specific frameshifting frequencies will be of utility for applications such as double coding DNA sequences by expanding the codable space into the -1 frame. Programmed shifting into the -1 frame to bypass a stop codon allows labeling of a protein pool with a fixed stoichiometry of fusion protein, as well as the construction of multi-enzyme expression constructs with specific expression ratios. A detailed understanding of the structural basis of programmed frameshifting will provide the opportunities to rationally design frameshifting elements with a wide range of applications in synthetic biology, including signals that are regulated by small ligands.","PeriodicalId":90376,"journal":{"name":"Translation (Austin, Tex.)","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87296783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Modifying the maker: Oxygenases target ribosome biology. 修饰制造者：加氧酶以核糖体生物学为目标。

Translation (Austin, Tex.) Pub Date : 2015-02-03 eCollection Date: 2015-01-01 DOI: 10.1080/21690731.2015.1009331

Qinqin Zhuang, Tianshu Feng, Mathew L Coleman

引用次数: 0

Regulation of global and specific mRNA translation by the mTOR signaling pathway 通过mTOR信号通路调控全局和特异性mRNA翻译

Translation (Austin, Tex.) Pub Date : 2015-01-02 DOI: 10.4161/21690731.2014.983402

N. Nandagopal, Philippe P Roux

引用次数: 115

Paradigms of ribosome synthesis: Lessons learned from ribosomal proteins 核糖体合成的范例:从核糖体蛋白中吸取的教训

Translation (Austin, Tex.) Pub Date : 2015-01-02 DOI: 10.4161/21690731.2014.975018

M. Gamalinda, J. Woolford

引用次数: 23

Summary of the 2014 meeting on translational control 2014年平移控制会议纪要

Translation (Austin, Tex.) Pub Date : 2015-01-02 DOI: 10.1080/21690731.2015.1037581

J. Hershey, V. Polunovsky

引用次数: 0