A. K. Tiwari, M. B. Mandal, Sadhana Kanoo, A. Alex, A. Jariwala, S. Deshpande
{"title":"Isolation of 5-Hydroxytryptamine from the Venom of the Indian Red Scorpion (Mesobuthus tamulus)","authors":"A. K. Tiwari, M. B. Mandal, Sadhana Kanoo, A. Alex, A. Jariwala, S. Deshpande","doi":"10.2174/1875414700801010001","DOIUrl":"https://doi.org/10.2174/1875414700801010001","url":null,"abstract":"The present study was undertaken to isolate 5-hydroxytryptamine (5-HT) from crude Indian red scorpion (Mesobuthus tamulus; MBT) venom as it has not been reported yet. Therefore, with the help of bioassay (using gastric fundus contractions) and fluorimetric assay methods, the 5-HT content of crude MBT venom (CV) was determined. The concentration of 5-HT in CV by either of the methods was different (117 times). Further, a 5-HT antagonist methysergide partially blocked the CV-induced gastric fundus contractions. Hence, for isolation and determination of 5-HT in crude MBT venom, CV was subjected to Sephadex gel (G 75-125) filtration chromatography. The eluates were screened for op- tical density (OD) at 280 nm, lethality and gastric fundus contractions. Two peaks of enhanced gastric fundus contractions were observed between 26-70 ml and 90 -110 ml of eluate volumes. As the first peak (between 26-70 ml) eluates exhib- ited high OD and lethality, further analysis was not undertaken. The second peak (between 90-110 ml) eluates exhibited very low OD and were non-lethal. On bioassay, the 5-HT content of pooled eluates of second peak was 0.0398 ± 0.008 � g/ml and by fluorimetric assay it was 0.0396 ± 0.007 � g/ml. The 5-HT concentration of crude MBT venom per unit mass was 0.0175 ± 0.0035 �g/mg by bioassay and 0.0174 ± 0.0031 �g/mg by fluorimetric assay. Further, the gastric fundus contractions elicited by second peak eluates were blocked by methysergide (1.0 �M). Thus, the second peak eluates of crude MBT venom contain 5-HT and its concentration was about 0.0174 � g/mg.","PeriodicalId":90367,"journal":{"name":"The open toxinology journal","volume":"1 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2008-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68118225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}