Biopolymers & Cell最新文献_第7页

In vitro and in vivo investigations on anti-influenza effect of adamantyl (alkyl, cycloalkyl) derivatives of aminopropanol-2 氨基丙醇-2金刚烷基(烷基、环烷基)衍生物抗流感作用的体内外研究

Biopolymers & Cell Pub Date : 2018-01-07 DOI: 10.7124/bc.000968

O. Voloshchuk, Y. V. Korotkiy, S. Rybalko, D. Starosila, V. Shirobokov

{"title":"In vitro and in vivo investigations on anti-influenza effect of adamantyl (alkyl, cycloalkyl) derivatives of aminopropanol-2","authors":"O. Voloshchuk, Y. V. Korotkiy, S. Rybalko, D. Starosila, V. Shirobokov","doi":"10.7124/bc.000968","DOIUrl":"https://doi.org/10.7124/bc.000968","url":null,"abstract":"Aim. To establish anti-influenza activities for 8 compounds of adamantyl (alkyl, cycloalkyl) derivatives of aminopropanol-2 in vitro and in vivo investigations. Methods. The antiviral action of compounds was determined in vitro by reduction of infectious titer of the influenza virus in the Madin-Darby Canine Kidney (MDCK) cell culture and in vivo on the model of influenza pneumonia in mice. Results. Three of eight studied compounds inhibit the reproduction of the influenza virus strain A/FM/1/47(H1N1) by more than 2 lg ID 50 in the concentration range from < 0.39 µg/ml (the compounds 30 and 33) to 1.56 µg/ml (the compound 5), their chemotherapeutic indices are 256 and 16, respectively. The experiments in vivo showed that in prophylactic scheme the efficiency index (EI) of the compounds 5 and 30 is 60 %; in therapeutic scheme EI of the compounds 5 and 33 is 100 % that is two times higher, than for rimantadine. Conclusions. High chemotherapeutic indices of the compounds 30, 33 and their ability to sup-press the influenza virus reproduction in the MDCK cell culture by more than 2 lg ID 50 indicate the anti-influenza activity of these compounds. It has been also confirmed by in vivo experiment.","PeriodicalId":9017,"journal":{"name":"Biopolymers & Cell","volume":"10 1","pages":"453-462"},"PeriodicalIF":0.0,"publicationDate":"2018-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82442003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Двухцелевая стратегия выявления Chlamydia trachomatis с помощью ПЦР в реальном времени Chlamydia trachomatis实时识别双目标策略

Biopolymers & Cell Pub Date : 2018-01-01 DOI: 10.7124/BC.000976

Я. А. Витренко, О. Н. Дерябин

引用次数: 0

Objective clustering inductive technology of gene expression profiles based on sota clustering algorithm 目的基于sota聚类算法的基因表达谱聚类诱导技术

Biopolymers & Cell Pub Date : 2017-10-31 DOI: 10.7124/BC.000961

S. Babichev, A. Gozhyj, A. Kornelyuk, V. Lytvynenko

引用次数: 23

Expression of epithelial-mesenchymal transition-related genes in prostate tumours 前列腺肿瘤上皮-间质转化相关基因的表达

Biopolymers & Cell Pub Date : 2017-10-31 DOI: 10.7124/BC.00095E

G. Gerashchenko, O. Mankovska, A. Dmitriev, L. Mevs, E. E. Rosenberg, M. Pikul, M. V. Marynychenko, O. Gryzodub, E. Stakhovsky, V. Kashuba

{"title":"Expression of epithelial-mesenchymal transition-related genes in prostate tumours","authors":"G. Gerashchenko, O. Mankovska, A. Dmitriev, L. Mevs, E. E. Rosenberg, M. Pikul, M. V. Marynychenko, O. Gryzodub, E. Stakhovsky, V. Kashuba","doi":"10.7124/BC.00095E","DOIUrl":"https://doi.org/10.7124/BC.00095E","url":null,"abstract":"Aim. To detect expression of EMT-related genes in prostate tumor samples and analyze a possible correlation between the gene expression level and clinical characteristics of prostate cancer in different groups. Methods. Expression of 19 genes was analyzed in 37 frozen samples of prostate cancer tissues at different tumor stages and Gleason scores, 37 paired conventionally normal prostate tissues and 20 samples of prostate adenomas, using quantitative PCR. Results. We have found that nine genes were expressed differently in benign and malignant prostate tumors, namely AR (isoform 1), AR (isoform 2), PTEN , VIM, MMP9, KRT18, PCA3, HOTAIR and SCHLAP1 . When different tumor stages were compared, we could identify six differentially expressed genes: KRT18, MMP9, VIM, PCA3, HOTAIR and SCHLAP1 ; when samples of tumors with different Gleason score were compared, we found that eight genes were expressed differently: AR (isoform 1), CDH1, KRT18, MMP9, OCLN, PCA3, HOTAIR and SCHLAP1 . The datahad a high level of heterogeneity potentially due to various molecular subtypes of prostate cancer, i.e. a luminal subtype with a high expression of CDH1, OCLN, AR(1 isof), KRT18, NKX3-1 and PSA ; the stem-like subtype with the high expression of mesenchymal markers CDH2, FN1 and VIM and low expression of the epithelial markers. It is noteworthy that lncRNAs were specifically expressed in these two molecular subtypes. Conclusions. EMT-related genes were differentially expressed in benign and malignant prostate tumors. High heterogeneity of expression levels, especially in adenocarcinoma groups, might suggest the existence of at least two different molecular subtypes, luminal and stem-like. Further experiments are necessary for specification of the molecular subtypes of prostate adenocarcinoma.","PeriodicalId":9017,"journal":{"name":"Biopolymers & Cell","volume":"40 1","pages":"335-355"},"PeriodicalIF":0.0,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85094311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Association of genetic polymorphism with the mutation status of the BRCA1/2 genes in spontanous breast cancer 基因多态性与自发性乳腺癌BRCA1/2基因突变状态的关系

Biopolymers & Cell Pub Date : 2017-10-31 DOI: 10.7124/BC.000962

S. I. Polinyk, L. Rybchenko, S. Klymenko, L. M. Zaharceva, B. Klimuk

引用次数: 0

Nitric oxide as the main multifunctional regulator of immunocompetent and endothelial cells 一氧化氮是免疫活性细胞和内皮细胞的主要多功能调节剂

Biopolymers & Cell Pub Date : 2017-10-31 DOI: 10.7124/BC.000957

O. V. Sadlyak, M. Kolishetska, M. Baida, Khristina Musij-Sementsiv, N. G. Sementsiv, R. Lesyk

引用次数: 0

Synthesis and biological evaluation of novel amino-substituted derivatives of pyrido[2,3-d]pyrimidine as inhibitors of protein kinase CK2 新型吡啶[2,3-d]嘧啶氨基取代衍生物作为蛋白激酶CK2抑制剂的合成及生物学评价

Biopolymers & Cell Pub Date : 2017-10-31 DOI: 10.7124/BC.000960

A. N. Zinchenko, L. Muzychka, O. B. Smolii, V. Bdzhola, M. Protopopov, S. Yarmoluk