Optical nanoscopy最新文献

筛选

英文中文

A high-density 3D localization algorithm for stochastic optical reconstruction microscopy. 随机光学重建显微镜高密度三维定位算法。

Optical nanoscopy Pub Date : 2012-01-01 DOI: 10.1186/2192-2853-1-6

Hazen Babcock, Yaron M Sigal, Xiaowei Zhuang

{"title":"A high-density 3D localization algorithm for stochastic optical reconstruction microscopy.","authors":"Hazen Babcock, Yaron M Sigal, Xiaowei Zhuang","doi":"10.1186/2192-2853-1-6","DOIUrl":"https://doi.org/10.1186/2192-2853-1-6","url":null,"abstract":"Background: Stochastic optical reconstruction microscopy (STORM) and related methods achieves sub-diffraction-limit image resolution through sequential activation and localization of individual fluorophores. The analysis of image data from these methods has typically been confined to the sparse activation regime where the density of activated fluorophores is sufficiently low such that there is minimal overlap between the images of adjacent emitters. Recently several methods have been reported for analyzing higher density data, allowing partial overlap between adjacent emitters. However, these methods have so far been limited to two-dimensional imaging, in which the point spread function (PSF) of each emitter is assumed to be identical.Methods: In this work, we present a method to analyze high-density super-resolution data in three dimensions, where the images of individual fluorophores not only overlap, but also have varying PSFs that depend on the z positions of the fluorophores.Results and conclusion: This approach can accurately analyze data sets with an emitter density five times higher than previously possible with sparse emitter analysis algorithms. We applied this algorithm to the analysis of data sets taken from membrane-labeled retina and brain tissues which contain a high-density of labels, and obtained substantially improved super-resolution image quality.","PeriodicalId":90036,"journal":{"name":"Optical nanoscopy","volume":"1 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2192-2853-1-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32843233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 145

首页上一页