Optical nanoscopy最新文献

Active Microscope Stabilization in Three Dimensions Using Image Correlation. 利用图像相关的三维主动显微镜稳定。

Optical nanoscopy Pub Date : 2013-04-18 DOI: 10.1186/2192-2853-2-3

Ryan McGorty, Daichi Kamiyama, Bo Huang

{"title":"Active Microscope Stabilization in Three Dimensions Using Image Correlation.","authors":"Ryan McGorty, Daichi Kamiyama, Bo Huang","doi":"10.1186/2192-2853-2-3","DOIUrl":"https://doi.org/10.1186/2192-2853-2-3","url":null,"abstract":"Background: Super-resolution microscopy techniques are often extremely susceptible to sample drift due to their high spatial resolution and the long time needed for data acquisition. While several techniques for stabilizing against drift exist, many require complicated additional hardware or intrusive sample preparations. We introduce a method that requires no additional sample preparation, is simple to implement and simultaneously corrects for x, y and z drift.Results: We use bright-field images of the specimen itself to calculate drift in all three dimensions: x, y and z. Bright-field images are acquired on an inexpensive CCD. By correlating each acquired bright-field image with an in-focus and two out-of-focus reference images we determine and actively correct for drift at rates of a few Hertz. This method can maintain stability to within 10 nm for x and y and 20 nm for z over several minutes.Conclusion: Our active drift stabilization system is capable of simultaneously compensating x, y and z drift through an image-based correlation method that requires no special sample treatment or extensive microscope modifications. While other techniques may provide better stability, especially for higher frequency drift, our method is easy to implement and widely applicable in terms of both sample type and microscopy technique.","PeriodicalId":90036,"journal":{"name":"Optical nanoscopy","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2192-2853-2-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31992115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 74

Resolving the spatial relationship between intracellular components by dual color super resolution optical fluctuations imaging (SOFI). 通过双色超分辨率光学波动成像（SOFI）解析细胞内成分之间的空间关系。

Optical nanoscopy Pub Date : 2013-02-25 DOI: 10.1186/2192-2853-2-2

Maria Elena Gallina, Jianmin Xu, Thomas Dertinger, Adva Aizer, Yaron Shav-Tal, Shimon Weiss

{"title":"Resolving the spatial relationship between intracellular components by dual color super resolution optical fluctuations imaging (SOFI).","authors":"Maria Elena Gallina, Jianmin Xu, Thomas Dertinger, Adva Aizer, Yaron Shav-Tal, Shimon Weiss","doi":"10.1186/2192-2853-2-2","DOIUrl":"10.1186/2192-2853-2-2","url":null,"abstract":"Background: Multi-color super-resolution (SR) imaging microscopy techniques can resolve ultrastructura relationships between- and provide co-localization information of- different proteins inside the cell or even within organelles at a higher resolution than afforded by conventional diffraction-limited imaging. While still very challenging, important SR colocalization results have been reported in recent years using STED, PALM and STORM techniques.Results: In this work, we demonstrate dual-color Super Resolution Optical Fluctuations Imaging (SOFI) using a standard far-field fluorescence microscope and different color blinking quantum dots. We define the spatial relationship between hDcp1a, a processing body (P-body, PB) protein, and the tubulin cytoskeletal network. Our finding could open up new perspectives on the role of the cytoskeleton in PB formation and assembly. Further insights into PB internal organization are also reported and discussed.Conclusions: Our results demonstrate the suitability and facile use of multi-color SOFI for the investigation of intracellular ultrastructures.","PeriodicalId":90036,"journal":{"name":"Optical nanoscopy","volume":"2 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855418/pdf/nihms455154.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31943355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blind assessment of localisation microscope image resolution 定位显微镜图像分辨率的盲评估

Optical nanoscopy Pub Date : 2012-12-10 DOI: 10.1186/2192-2853-1-12

E. Rees, M. Erdélyi, D. Pinotsi, A. Knight, D. Metcalf, C. Kaminski

引用次数: 39

GraspJ: an open source, real-time analysis package for super-resolution imaging GraspJ:用于超分辨率成像的开源实时分析包

Optical nanoscopy Pub Date : 2012-12-05 DOI: 10.1186/2192-2853-1-11

Norman Brede, M. Lakadamyali

引用次数: 28

SOFI-based 3D superresolution sectioning with a widefield microscope. 基于sofi的宽视场显微镜三维超分辨切片。

Optical nanoscopy Pub Date : 2012-12-01 DOI: 10.1186/2192-2853-1-2

Thomas Dertinger, Jianmin Xu, Omeed Foroutan Naini, Robert Vogel, Shimon Weiss