Metallomics最新文献_第9页

Long-term suboptimal dietary trace element supply does not affect trace element homeostasis in murine cerebellum. 长期膳食微量元素供应不足不会影响小鼠小脑的微量元素稳态。

IF 3.4 3区生物学

Metallomics Pub Date : 2024-02-07 DOI: 10.1093/mtomcs/mfae003

Sharleen Friese, Giovanna Ranzini, Max Tuchtenhagen, Kristina Lossow, Barbara Hertel, Gabriele Pohl, Franziska Ebert, Julia Bornhorst, Anna Patricia Kipp, Tanja Schwerdtle

{"title":"Long-term suboptimal dietary trace element supply does not affect trace element homeostasis in murine cerebellum.","authors":"Sharleen Friese, Giovanna Ranzini, Max Tuchtenhagen, Kristina Lossow, Barbara Hertel, Gabriele Pohl, Franziska Ebert, Julia Bornhorst, Anna Patricia Kipp, Tanja Schwerdtle","doi":"10.1093/mtomcs/mfae003","DOIUrl":"10.1093/mtomcs/mfae003","url":null,"abstract":"The ageing process is associated with alterations of systemic trace element (TE) homeostasis increasing the risk, e.g. neurodegenerative diseases. Here, the impact of long-term modulation of dietary intake of copper, iron, selenium, and zinc was investigated in murine cerebellum. Four- and 40-wk-old mice of both sexes were supplied with different amounts of those TEs for 26 wk. In an adequate supply group, TE concentrations were in accordance with recommendations for laboratory mice while suboptimally supplied animals received only limited amounts of copper, iron, selenium, and zinc. An additional age-adjusted group was fed selenium and zinc in amounts exceeding recommendations. Cerebellar TE concentrations were measured by inductively coupled plasma-tandem mass spectrometry. Furthermore, the expression of genes involved in TE transport, DNA damage response, and DNA repair as well as selected markers of genomic stability [8-oxoguanine, incision efficiency toward 8-oxoguanine, 5-hydroxyuracil, and apurinic/apyrimidinic sites and global DNA (hydroxy)methylation] were analysed. Ageing resulted in a mild increase of iron and copper concentrations in the cerebellum, which was most pronounced in the suboptimally supplied groups. Thus, TE changes in the cerebellum were predominantly driven by age and less by nutritional intervention. Interestingly, deviation from adequate TE supply resulted in higher manganese concentrations of female mice even though the manganese supply itself was not modulated. Parameters of genomic stability were neither affected by age, sex, nor diet. Overall, this study revealed that suboptimal dietary TE supply does not substantially affect TE homeostasis in the murine cerebellum.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10873500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139649837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiproliferative activity of platinum(II) and copper(II) complexes containing novel biquinoxaline ligands. 含有新型双喹喔啉配体的铂（II）和铜（II）配合物的抗增殖活性。

IF 3.4 3区生物学

Metallomics Pub Date : 2024-02-07 DOI: 10.1093/mtomcs/mfae001

Hager Sadek El-Beshti, Zuhal Gercek, Hakan Kayi, Yasemin Yildizhan, Yuksel Cetin, Zelal Adigüzel, Gamze Güngör, Şeniz Özalp-Yaman

{"title":"Antiproliferative activity of platinum(II) and copper(II) complexes containing novel biquinoxaline ligands.","authors":"Hager Sadek El-Beshti, Zuhal Gercek, Hakan Kayi, Yasemin Yildizhan, Yuksel Cetin, Zelal Adigüzel, Gamze Güngör, Şeniz Özalp-Yaman","doi":"10.1093/mtomcs/mfae001","DOIUrl":"10.1093/mtomcs/mfae001","url":null,"abstract":"Nowadays, cancer represents one of the major causes of death in humans worldwide, which renders the quest for new and improved antineoplastic agents to become an urgent issue in the field of biomedicine and human health. The present research focuses on the synthesis of 2,3,2',3'-tetra(pyridin-2-yl)-6,6'-biquinoxaline) and (2,3,2',3'-tetra(thiophen-2-yl)-6,6'-biquinoxaline) containing copper(II) and platinum(II) compounds as prodrug candidates. The binding interaction of these compounds with calf thymus DNA (CT-DNA) and human serum albumin were assessed with UV titration, thermal decomposition, viscometric, and fluorometric methods. The thermodynamical parameters and the temperature-dependent binding constant (K'b) values point out to spontaneous interactions between the complexes and CT-DNA via the van der Waals interactions and/or hydrogen bonding, except Cu(ttbq)Cl2 for which electrostatic interaction was proposed. The antitumor activity of the complexes against several human glioblastomata, lung, breast, cervix, and prostate cell lines were investigated by examining cell viability, oxidative stress, apoptosis-terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, in vitro migration and invasion, in vitro-comet DNA damage, and plasmid DNA interaction assays. The U87 and HeLa cells were investigated as the cancer cells most sensitive to our complexes. The exerted cytotoxic effect of complexes was attributed to the formation of the reactive oxygen species in vitro. It is clearly demonstrated that Cu(ttbq)Cl2, Pt(ttbq)Cl2, and Pt(tpbq)Cl2 have the highest DNA degradation potential and anticancer effect among the tested complexes by leading apoptosis. The wound healing and invasion analysis results also supported the higher anticancer activity of these two compounds.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10849753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for decreased copper associated with demyelination in the corpus callosum of cuprizone-treated mice. 铜绿素处理过的小鼠胼胝体中与脱髓鞘有关的铜减少的证据。

IF 3.4 3区生物学

Metallomics Pub Date : 2024-01-05 DOI: 10.1093/mtomcs/mfad072

James B W Hilton, Kai Kysenius, Jeffrey R Liddell, Stephen W Mercer, Dominic J Hare, Gojko Buncic, Bence Paul, YouJia Wang, Simon S Murray, Trevor J Kilpatrick, Anthony R White, Paul S Donnelly, Peter J Crouch

{"title":"Evidence for decreased copper associated with demyelination in the corpus callosum of cuprizone-treated mice.","authors":"James B W Hilton, Kai Kysenius, Jeffrey R Liddell, Stephen W Mercer, Dominic J Hare, Gojko Buncic, Bence Paul, YouJia Wang, Simon S Murray, Trevor J Kilpatrick, Anthony R White, Paul S Donnelly, Peter J Crouch","doi":"10.1093/mtomcs/mfad072","DOIUrl":"10.1093/mtomcs/mfad072","url":null,"abstract":"Demyelination within the central nervous system (CNS) is a significant feature of debilitating neurological diseases such as multiple sclerosis and administering the copper-selective chelatorcuprizone to mice is widely used to model demyelination in vivo. Conspicuous demyelination within the corpus callosum is generally attributed to cuprizone's ability to restrict copper availability in this vulnerable brain region. However, the small number of studies that have assessed copper in brain tissue from cuprizone-treated mice have produced seemingly conflicting outcomes, leaving the role of CNS copper availability in demyelination unresolved. Herein we describe our assessment of copper concentrations in brain samples from mice treated with cuprizone for 40 d. Importantly, we applied an inductively coupled plasma mass spectrometry methodology that enabled assessment of copper partitioned into soluble and insoluble fractions within distinct brain regions, including the corpus callosum. Our results show that cuprizone-induced demyelination in the corpus callosum was associated with decreased soluble copper in this brain region. Insoluble copper in the corpus callosum was unaffected, as were pools of soluble and insoluble copper in other brain regions. Treatment with the blood-brain barrier permeant copper compound CuII(atsm) increased brain copper levels and this was most pronounced in the soluble fraction of the corpus callosum. This effect was associated with significant mitigation of cuprizone-induced demyelination. These results provide support for the involvement of decreased CNS copper availability in demyelination in the cuprizone model. Relevance to human demyelinating disease is discussed.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139096747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclometalated ruthenium complexes overcome cisplatin resistance through PI3K/mTOR/Nrf2 signaling pathway. 环金属化钌复合物通过 PI3K/mTOR/Nrf2 信号通路克服顺铂耐药性

IF 3.4 3区生物学

Metallomics Pub Date : 2024-01-05 DOI: 10.1093/mtomcs/mfae002

Lanmei Chen, Wenzhu Yu, Hong Tang, Shenting Zhang, Jie Wang, Qianqian Ouyang, Miao Guo, Xufeng Zhu, Zunnan Huang, Jincan Chen

{"title":"Cyclometalated ruthenium complexes overcome cisplatin resistance through PI3K/mTOR/Nrf2 signaling pathway.","authors":"Lanmei Chen, Wenzhu Yu, Hong Tang, Shenting Zhang, Jie Wang, Qianqian Ouyang, Miao Guo, Xufeng Zhu, Zunnan Huang, Jincan Chen","doi":"10.1093/mtomcs/mfae002","DOIUrl":"10.1093/mtomcs/mfae002","url":null,"abstract":"Currently, cisplatin resistance remains a primary clinical obstacle in the successful treatment of non-small cell lung cancer. Here, we designed, synthesized, and characterized two novel cyclometalated Ru(II) complexes, [Ru(bpy)2(1-Ph-7-OCH3-IQ)] (PF6) (bpy = 2,2'-bipyridine, IQ = isoquinoline, RuIQ7)and [Ru(bpy)2(1-Ph-6,7-(OCH3)2-IQ)] (PF6) (RuIQ8). As experimental controls, we prepared complex [Ru(bpy)2(1-Ph-IQ)](PF6) (RuIQ6) lacking a methoxy group in the main ligand. Significantly, complexes RuIQ6-8 displayed higher in vitro cytotoxicity when compared to ligands, precursor cis-[Ru(bpy)2Cl2], and clinical cisplatin. Mechanistic investigations revealed that RuIQ6-8 could inhibit cell proliferation by downregulating the phosphorylation levels of Akt and mTOR proteins, consequently affecting the rapid growth of human lung adenocarcinoma cisplatin-resistant cells A549/DDP. Moreover, the results from qRT-PCR demonstrated that these complexes could directly suppress the transcription of the NF-E2-related factor 2 gene, leading to the inhibition of downstream multidrug resistance-associated protein 1 expression and effectively overcoming cisplatin resistance. Furthermore, the relationship between the chemical structures of these three complexes and their anticancer activity, ability to induce cell apoptosis, and their efficacy in overcoming cisplatin resistance has been thoroughly examined and discussed. Notably, the toxicity test conducted on zebrafish embryos indicated that the three Ru-IQ complexes displayed favorable safety profiles. Consequently, the potential of these developed compounds as innovative therapeutic agents for the efficient and low-toxic treatment of NSCLC appears highly promising.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TauSTED super-resolution imaging of labile iron in primary hippocampal neurons. 原发性海马神经元中易变铁的 TauSTED 超分辨率成像。

IF 3.4 3区生物学

Metallomics Pub Date : 2024-01-05 DOI: 10.1093/mtomcs/mfad074

Aiyarin Kittilukkana, Asuncion Carmona, Chalermchai Pilapong, Richard Ortega

{"title":"TauSTED super-resolution imaging of labile iron in primary hippocampal neurons.","authors":"Aiyarin Kittilukkana, Asuncion Carmona, Chalermchai Pilapong, Richard Ortega","doi":"10.1093/mtomcs/mfad074","DOIUrl":"10.1093/mtomcs/mfad074","url":null,"abstract":"Iron dyshomeostasis is involved in many neurological disorders, particularly neurodegenerative diseases where iron accumulates in various brain regions. Identifying mechanisms of iron transport in the brain is crucial for understanding the role of iron in healthy and pathological states. In neurons, it has been suggested that iron can be transported by the axon to different brain regions in the form of labile iron; a pool of reactive and exchangeable intracellular iron. Here we report a novel approach to imaging labile ferrous iron, Fe(II), in live primary hippocampal neurons using confocal and TauSTED (stimulated emission depletion) microscopy. TauSTED is based on super-resolution STED nanoscopy, which combines high spatial resolution imaging (<40 nm) with fluorescence lifetime information, thus reducing background noise and improving image quality. We applied TauSTED imaging utilizing biotracker FerroFarRed Fe(II) and found that labile iron was present as submicrometric puncta in dendrites and axons. Some of these iron-rich structures are mobile and move along neuritic pathways, arguing for a labile iron transport mechanism in neurons. This super-resolution imaging approach offers a new perspective for studying the dynamic mechanisms of axonal and dendritic transport of iron at high spatial resolution in living neurons. In addition, this methodology could be transposed to the imaging of other fluorescent metal sensors.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global deletome profile of Saccharomyces cerevisiae exposed to lithium 暴露于锂的酿酒酵母的全球脱粒体概况

IF 3.4 3区生物学

Metallomics Pub Date : 2023-12-23 DOI: 10.1093/mtomcs/mfad073

Nicolas Fierling, Patrick Billard, Pascale Bauda, Damien Blaudez

{"title":"Global deletome profile of Saccharomyces cerevisiae exposed to lithium","authors":"Nicolas Fierling, Patrick Billard, Pascale Bauda, Damien Blaudez","doi":"10.1093/mtomcs/mfad073","DOIUrl":"https://doi.org/10.1093/mtomcs/mfad073","url":null,"abstract":"\u0000 The increasing use of lithium (Li) in new technologies raises the question of its impact on living microorganisms. In the present study, we aimed to identify putative Li targets and resistance mechanisms in the yeast model Saccharomyces cerevisiae using a deletomic approach based on the screening of a collection of 4,733 knockout mutants under Li exposure. This screening highlighted 60 mutants resistant to Li and 124 mutants sensitive to Li. Through functional enrichment analyses, transport systems and catabolite repression were identified as playing a central role in cell resistance to toxic concentrations of Li. In contrast, the AKT/protein kinase B family, signal transduction or cell communication were identified as potential toxic targets of Li. The majority of the mutants with a Li-sensitive phenotype were also sensitive to other alkali and alkaline-earth metals, whereas the Li-resistance phenotype was mostly resistant to Na but poorly resistant to other metals. A comparison with the results of deletomics studies carried out in the presence of other metals highlighted Li-specific phenotypes. Three genes (NAM7, NMD2, UPF3) of the nonsense-mediated decay pathway were specifically involved in resistance to Li. In contrast, mutants with the NCA2, SPT20, GCN5, YOR376W, YPK3, and DCW1 genes deleted were specifically sensitive to Li. These genes encode various functions from putative mannosidase to constitution of the Spt-Ada-Gcn5 acetyltransferase complex. This work provides a better understanding of potential specific resistance mechanisms and cellular targets of Li in yeast.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":"13 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138943761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of genes involved in micronutrients and toxic metals detoxification in Brassica napus by genome-wide cDNA library screening. 利用全基因组cDNA文库筛选甘蓝型油菜微量营养素和有毒金属解毒相关基因。

IF 3.4 3区生物学

Metallomics Pub Date : 2023-12-09 DOI: 10.1093/mtomcs/mfad068

Jia-Shi Peng, Xue-Jie Zhang, Jia-Ni Xiong, Ying Zhou, Wei-Li Wang, Si-Ying Chen, Da-Wei Zhang, Tian-Yu Gu

引用次数: 0

Expression of Concern: Characterising the spatial and temporal brain metal profile in a mouse model of tauopathy. 表达关注：牛磺酸脑病小鼠模型的空间和时间脑金属分布特征。

IF 2.9 3区生物学

Metallomics Pub Date : 2023-12-09 DOI: 10.1093/mtomcs/mfad071

引用次数: 0

The transporter PHO84/NtPT1 is a target of aluminum to affect phosphorus absorption in Saccharomyces cerevisiae and Nicotiana tabacum L. 转运体PHO84/NtPT1是铝影响酿酒酵母和烟草对磷吸收的靶标。

IF 3.4 3区生物学

Metallomics Pub Date : 2023-12-09 DOI: 10.1093/mtomcs/mfad069

Zhiwei Huang, Shixuan Zhang, Ranran Chen, Qian Zhu, Ping Shi, Yuhu Shen

{"title":"The transporter PHO84/NtPT1 is a target of aluminum to affect phosphorus absorption in Saccharomyces cerevisiae and Nicotiana tabacum L.","authors":"Zhiwei Huang, Shixuan Zhang, Ranran Chen, Qian Zhu, Ping Shi, Yuhu Shen","doi":"10.1093/mtomcs/mfad069","DOIUrl":"10.1093/mtomcs/mfad069","url":null,"abstract":"The molecular mechanism of aluminum toxicity in biological systems is not completely understood. Saccharomyces cerevisiae is one of the most used model organisms in the study of environmental metal toxicity. Using an unbiased metallomic approach in yeast, we found that aluminum treatment caused phosphorus deprivation, and the lack of phosphorus increased as the pH of the environment decreased compared to the control strain. By screening the phosphate signaling and response pathway (PHO pathway) in yeast with the synthetic lethality of a new phosphorus-restricted aluminum-sensitive gene, we observed that pho84Δ mutation conferred severe growth defect to aluminum under low-phosphorus conditions, and the addition of phosphate alleviated this sensitivity. Subsequently, the data showed that PHO84 determined the intracellular aluminum-induced phosphorus deficiency, and the expression of PHO84 was positively correlated with aluminum stress, which was mediated by phosphorus through the coordinated regulation of PHO4/PHO2. Moreover, aluminum reduced phosphorus absorption and inhibited tobacco plant growth in acidic media. In addition, the high-affinity phosphate transporter NtPT1 in tobacco exhibited similar effects to PHO84, and overexpression of NtPT1 conferred aluminum resistance in yeast cells. Taken together, positive feedback regulation of the PHO pathway centered on the high-affinity phosphate transporters is a highly conservative mechanism in response to aluminum toxicity. The results may provide a basis for aluminum-resistant microorganisms or plant engineering and acidic soil treatment.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138294178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans 组蛋白-5 与细胞铜相互作用，促进对白色念珠菌的抗真菌活性

IF 3.4 3区生物学

Metallomics Pub Date : 2023-12-07 DOI: 10.1093/mtomcs/mfad070

Joanna X Campbell, Natalie B Schulte, Barry Lai, Hugh H Harris, K. Franz

{"title":"Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans","authors":"Joanna X Campbell, Natalie B Schulte, Barry Lai, Hugh H Harris, K. Franz","doi":"10.1093/mtomcs/mfad070","DOIUrl":"https://doi.org/10.1093/mtomcs/mfad070","url":null,"abstract":"\u0000 Histatin-5 (Hist-5) is an antimicrobial peptide found in human saliva that functions to defend the oral cavity from microbial infections, such as those caused by the fungal pathogen Candida albicans (C. albicans). Hist-5 can bind Cu in multiple oxidation states, Cu2+ and Cu+ in vitro, and supplemental Cu2+ has been shown to improve the fungicidal activity of the peptide against C. albicans in culture. However, the exact role of Cu on the antifungal activity of Hist-5 and whether direct peptide–Cu interactions occur intracellularly has yet to be fully determined. Here, we used a combination of fluorescence spectroscopy and confocal microscopy experiments to show reversible Cu-dependent quenching of a fluorescent Hist-5 analogue, Hist-5*, indicating a direct interaction between Hist-5 and intracellular Cu. X-ray fluorescence microscopy images revealed peptide-induced changes to cellular Cu distribution and cell-associated Cu content. These data support a model in which Hist-5 can facilitate the hyperaccumulation of Cu in C. albicans and directly interact with Cu intracellularly to increase the fungicidal activity of Hist-5.","PeriodicalId":89,"journal":{"name":"Metallomics","volume":"32 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138590209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0