Biopropal Industri最新文献

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PROSES PRODUKSI EMULSI VIRGIN COCONUT OIL (VCO) DENGAN FIKOSIANIN SEBAGAI ANTIOKSIDAN 处女椰子油的生产过程,作为抗氧化剂
Biopropal Industri Pub Date : 2020-12-14 DOI: 10.36974/JBI.V11I2.6245
Novi Nur Aidha, Siti Agustina, Eva Oktarina, Ira Setiawati
{"title":"PROSES PRODUKSI EMULSI VIRGIN COCONUT OIL (VCO) DENGAN FIKOSIANIN SEBAGAI ANTIOKSIDAN","authors":"Novi Nur Aidha, Siti Agustina, Eva Oktarina, Ira Setiawati","doi":"10.36974/JBI.V11I2.6245","DOIUrl":"https://doi.org/10.36974/JBI.V11I2.6245","url":null,"abstract":"Indonesia merupakan wilayah yang berpotensi untuk perkembangan budidaya mikroalga, salah satu diantaranya adalah Spirulina yang mengandung antioksidan fikosianin. Antioksidan fikosianin digunakan pada industri kosmetik, farmasi dan makanan. Pada diverifikasi produk, antioksidan fikosianin dapat dimanfaatkan dalam bentuk emulsi. Tujuan penelitian ini adalah melakukan dan mengamati proses produksi emulsi dengan variabel VCO dan  dengan fikosianin sebagai antiokisidan, fikosianin menggunakan metode homogenisasi dengan tekanan tinggi (High Pressure Homogenation). Pada penelitian ini, jenis emulsi yang dibuat adalah O/W (minyak dalam air)  dimana fase minyak berupa VCO dan larutan fikosianin berupa sebagai fase air. Emulsi yang dibuat terdiri dari 9 formula menggunakan Carboxyl Methyl Cellulose (CMC) sebagai emulsifier. Produk emulsi dilakukan karakterisasi yang terdiri dari analisa viskositas, pH. , kestabilan dan kadar antioksidannya. Rancangan penelitian menggunakan software Design Expert® 7.0 dengan variabel viskositas, pH. , kestabilan dan kadar antioksidan serta metode Central Composite Design (CCD) yang merupakan salah satu metode permukaan tanggap untuk mendapatkan emulsi yang berfungsi sebagai antioksidan. Hasil didapatkan bahwa formula terbaik adalah perbandingan VCO: fikosianin (31,25 : 131,25) dengan kadar antioksidan sebesar 0,07 mg vit C/g, pH 4,64 dengan viskositas sebesar 113 cps. Berdasarkan hasil tersebut, emulsi antioksidan dapat diaplikasikan untuk industri kosmetik, farmasi dan makanan.","PeriodicalId":8988,"journal":{"name":"Biopropal Industri","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41776997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PENGARUH KONSENTRASI KULIT SINGKONG DAN SUMBER NITROGEN TERHADAP PRODUKSI GLUKOAMILASE OLEH Aspergillus awamori KT-11PADA SUBMERGED FERMENTASI (Effect of Cassava Peelfor Glucoamylase Production by Aspergillus awamori KT-11 in Submerged Fermentation) 藤森曲霉菌KT-11PADA提交的含氮浓缩葡糖淀粉酶概述(藤森曲菌KT-11在深层发酵中生产木薯皮葡糖淀粉酶的影响)
Biopropal Industri Pub Date : 2020-12-11 DOI: 10.36974/JBI.V11I2.6079
U. Perwitasari, Siti Amanah, M. D. Wahidiyah, Nuryati, Ruth Melliawati, L. Kholida, A. Andriani, Ahmad Thontowi, Awan Purnawan, A. Fathoni, S. Hartati, Y. Lusini, Yopi
{"title":"PENGARUH KONSENTRASI KULIT SINGKONG DAN SUMBER NITROGEN TERHADAP PRODUKSI GLUKOAMILASE OLEH Aspergillus awamori KT-11PADA SUBMERGED FERMENTASI (Effect of Cassava Peelfor Glucoamylase Production by Aspergillus awamori KT-11 in Submerged Fermentation)","authors":"U. Perwitasari, Siti Amanah, M. D. Wahidiyah, Nuryati, Ruth Melliawati, L. Kholida, A. Andriani, Ahmad Thontowi, Awan Purnawan, A. Fathoni, S. Hartati, Y. Lusini, Yopi","doi":"10.36974/JBI.V11I2.6079","DOIUrl":"https://doi.org/10.36974/JBI.V11I2.6079","url":null,"abstract":"The expensive price of glucoamylase is due to the high cost of enzyme production. Utilization biomass is expected to be an alternative raw material to reduce the cost of producing enzymes without reducing the quality of the product. Aspergillus awamori KT-11 is known to be able to produce glucoamylase by utilizing biomass waste. This study aimed to optimize glucoamylase production from A. awamori KT-11 by utilizing cassava peel as an alternative substrate with variation nitrogen source through Submerged Fermentation (SmF). Variables carry out in this study were the concentration of cassava peel (5-30%), nitrogen sources (casein hydrolyzate, yeast extract and sodium nitrate). The results showed in the concentration of cassava peels and nitrogen sources affect glucoamylase production. The optimum glucoamylase activity was 3984,935 U/L in 10% of cassava peel in medium. The other results showed different sources of nitrogen significantly influence glucoamylase production. The addition of yeast extract increased glucoamylase activity to 4617,894 U / L. Keywords: Aspergillus awamori KT-11, glucoamylase, cassava peel, submerged fermentation (SmF) ABSTRAK Bahan baku yang mahal dalam produksi glukoamilase mengakibatkan harga jual glukoamilase tinggi. Pemanfaatan biomassa tinggi karbohidrat diharapkan dapat menjadi alternatif bahan baku untuk menurunkan biaya produksi enzim tanpa menurunkan kualitas dari produk yang dihasilkan. Aspergillus awamori KT-11 diketahui mampu memproduksi glukoamilase dengan memanfaatkan limbah biomassa. Tujuan penelitian ini adalah optimasi produksi glukoamilase dari A. awamori KT-11 dengan memanfaatkan limbah kulit singkong sebagai substrat alternatif dengan berbagai sumber nitrogen melalui Submerged Fermentation (SmF). Variabel yang dilakukan pada penelitian ini yaitu konsentrasi kulit singkong (5-30%), sumber nitrogen (kasein hidrolisat, yeast extract dan natrium nitrat). Hasil riset menunjukkan konsentrasi kulit singkong dan jenis sumber nitrogen mempengaruhi produksi glukoamilase. Aktivitas glukoamilase optimum diidentifikasi ketika konsentrasi substrat kulit singkong sebesar 10% yaitu 3984,935 U/L. Penambahan sumber nitrogen yang berbeda mempengaruhi produksi glukoamilase secara signifikan. Penambahan yeast extract meningkatkan aktivitas glukoamilase menjadi 4617,894 U/L. Kata kunci: Aspergillus awamori KT-11, glukoamilase, kulit singkong, submerged fermentasi (SmF)","PeriodicalId":8988,"journal":{"name":"Biopropal Industri","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48998457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
STUDI POLA KONSUMSI TEH DI INDONESIA UNTUK MENDUKUNG DIVERSIFIKASI PRODUK YANG BERKELANJUTAN (A Study of Tea Consumption Pattern in Indonesia Toward Sustainable Product Diversification) 面向可持续产品多样化的印尼茶叶消费模式研究
Biopropal Industri Pub Date : 2020-12-11 DOI: 10.36974/JBI.V11I2.6249
H. Prasetia, A. Setiawan, T. B. Bardant, Muryanto, A. Randy, M. S. Haq, Adhi Irianto Mastur, S. Harianto, N. Annisa, A. Sulaswatty
{"title":"STUDI POLA KONSUMSI TEH DI INDONESIA UNTUK MENDUKUNG DIVERSIFIKASI PRODUK YANG BERKELANJUTAN (A Study of Tea Consumption Pattern in Indonesia Toward Sustainable Product Diversification)","authors":"H. Prasetia, A. Setiawan, T. B. Bardant, Muryanto, A. Randy, M. S. Haq, Adhi Irianto Mastur, S. Harianto, N. Annisa, A. Sulaswatty","doi":"10.36974/JBI.V11I2.6249","DOIUrl":"https://doi.org/10.36974/JBI.V11I2.6249","url":null,"abstract":"Research to explore consumer perspective toward tea product requirements has been being emphasized around the globe, as well as the advancing variety of consumer demand and market competitiveness. Particularly in Indonesia, broader market needs and government relevant policies toward environmental consideration made these issues also need to be significantly addressed. This study aimed to analyze tea in consumption patterns Indonesia to support diversified sustainable products. T he research design was conducted using survey method. A questionnaire was developed using structured interviews , with the number of respondents as many as 188 people . This survey was accommodating general type of tea such as green tea, white tea, black tea, etc. The result showed that respondents’ preferences toward tea belong to tea bags the most, with percentage 57.45% , powdered tea 28.19% and ready to drink tea 14.36% . Respondents age of 31-40 years old most widely consumed tea everyday (1.90 cup.day -1 ). As many as 78.19% of respondents were chose to consumed plain tea, followed by milk added tea 12.23% and creamer added tea 9.57%.  F urther analysis using the Likelihood Ratio Test showed th at age, gender and occupation factors were not a significant factor in the variety of tea types. To support the sustainable tea industry consumer behavior is also very important to be studied because consumers usually ask for high production standards but with a cheap cost. Keywords: consumption pattern, preference, product diversification, sustainable industry, tea ABSTRAK Penelitian mengenai eksplorasi perspektif konsumen terhadap persyaratan produk teh telah menjadi perhatian dunia, seiring dengan meningkatnya permintaan konsumen dan daya saing pasar . Khususnya di Indonesia, kebutuhan pasar yang lebih luas dan kebijakan pemerintah yang relevan terhadap pertimbangan lingkungan membuat masalah ini juga perlu ditangani secara signifikan. Penelitian ini memiliki tujuan untuk menganalisis pola konsumsi teh di Indonesia untuk mendukung diversifikasi produk yang berkelanjutan. Desain penelitian dilakukan dengan menggunakan metode survei. Kuesioner dikembangkan menggunakan wawancara terstruktur , dengan jumlah responden sebanyak 188 orang . Survei ini mengakomodasi teh secara umum, seperti teh hijau, teh putih, teh hitam, dan lain-lain . Hasil penelitian menunjukkan preferensi responden terhadap teh yang paling dominan adalah jenis teh celup (57 , 45%) , teh serbuk (28,19%) dan teh siap minum (14,36%) . Responden dengan usia 31-40 tahun paling banyak menkonsumsi teh setiap hari (1,90 gelas.hari -1 ). Sebanyak 78,19% responden memilih konsumsi teh tawar setiap hari, diikuti oleh teh dengan susu 12,23% dan teh dengan krimer 9,57%. Hasil analisis lebih lanjut menggunakan Likelihood Ratio Test , didapatkan bahwa antara variabel usia, jenis kelamin dan pekerjaan tidak terdapat pengaruh yang signifikan dalam pemilihan jenis teh. Kata kunci: diversifikasi produk, industri berkelanjutan","PeriodicalId":8988,"journal":{"name":"Biopropal Industri","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42134596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
PENGAYAAN ANTIOKSIDAN UNTUK BERAS MERAH ARTIFICIAL DENGAN EKSTRAK PEWARNA MERAH ALAMI Rhoeo Discolor L. Her (Antioxidant Enrichment for Artificial Red Rice by Natural Red Pigment Extract From Rhoeo Discolor L. Her) PENGAYAAN ANTIOKSIDAN UNTUK BERAS MERAH ARTIFICIAL DENGAN EKSTRAK PEWARNA MERAH ALAMI Rhoeo Discolor L.Her
Biopropal Industri Pub Date : 2020-06-01 DOI: 10.36974/jbi.v11i1.5418
Husniati Husniati, J. Permana, T. Suhartati
{"title":"PENGAYAAN ANTIOKSIDAN UNTUK BERAS MERAH ARTIFICIAL DENGAN EKSTRAK PEWARNA MERAH ALAMI Rhoeo Discolor L. Her (Antioxidant Enrichment for Artificial Red Rice by Natural Red Pigment Extract From Rhoeo Discolor L. Her)","authors":"Husniati Husniati, J. Permana, T. Suhartati","doi":"10.36974/jbi.v11i1.5418","DOIUrl":"https://doi.org/10.36974/jbi.v11i1.5418","url":null,"abstract":"A rtificial rice is one of food diversification alternative from non-rice local carbohydrate s . Generally, artificial rice is a flour based using cassava, tapioca, corn and sago from Indonesia as raw material, with additional of micronutrients such as mineral and vitamin. This research aimed to apply the red pigment anthocyanin which contai n antioxidant s for artificial red rice production. Red pigment was extracted from A dam hawa (Rhoeo discolor L. Her) leaves in acid water solvent and predicted as cyanidin-3-galactose or peonidin-3-glucose compounds. Artificial red rice was tested its color degree based on the CIE (Commission Internationale de I'Eclairage) Lab method and the result indicated the red color brightness and its color stability lighter than natural red rice . Keywords: Anthocyanins, antioxidants, artificial rice, Rhoeo discolor L. Her ABSTRAK Beras artificial menjadi salah satu alternatif diversifikasi pangan berbahan baku karbohidrat lokal bukan beras. Umumnya, beras artificial menggunakan bahan baku berbasis tepung seperti singkong, tapioka, jagung dan sagu asal Indonesia, serta tambahan mikronutrien seperti mineral dan vitamin. Penelitian ini bertujuan untuk mengaplikasikan pewarna merah antosianin yang mengandung antioksidan untuk pembuatan beras merah artificial. Pewarna merah diekstrak dari bagian daun Adam hawa ( Rhoeo discolor L. Her) dalam pelarut air (akuades) yang mengandung asam dan diperkirakan sebagai senyawa sianidin-3-galaktosa atau peonidin-3-glukosa. Produk beras merah artificial diuji derajat warnanya mengacu nilai Lab CIE ( Commission Internationale de I’Eclairage ) dan jika dibandingkan dengan beras merah alami maka beras merah artificial menunjukkan skala pembacaan warna merah, kecerahan, serta kestabilan warna merahnya ke arah lebih terang. Kata kunci: Antioksidan, antosianin, beras artificial, Rhoeo discolor L. Her","PeriodicalId":8988,"journal":{"name":"Biopropal Industri","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44994484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
OPTIMASI WAKTU INKUBASI PRODUKSI BAHAN MINUMAN PROBIOTIK DARI UMBI GARUT (Maranta arundinacea L.) OLEH Lactobacillus fermentum SEBAGAI ANTIHIPERKOLESTEROLEMIA 优化时间,生产鳞茎旁益生菌饮料。fermentum Lactobacillus以抗胆固醇药
Biopropal Industri Pub Date : 2020-06-01 DOI: 10.36974/jbi.v11i1.5846
Harry Noviardi, S. Yuningtyas, Vira Yuniar
{"title":"OPTIMASI WAKTU INKUBASI PRODUKSI BAHAN MINUMAN PROBIOTIK DARI UMBI GARUT (Maranta arundinacea L.) OLEH Lactobacillus fermentum SEBAGAI ANTIHIPERKOLESTEROLEMIA","authors":"Harry Noviardi, S. Yuningtyas, Vira Yuniar","doi":"10.36974/jbi.v11i1.5846","DOIUrl":"https://doi.org/10.36974/jbi.v11i1.5846","url":null,"abstract":"Arrowroot tuber is one of the potential plants that has a high nutritional content so it can be the source of nutrition for the growth of Lactobacillus fermentum bacteria for probiotic drink production. A rrowroot fermentation  with L. fermentum is also known potentially reduce cholesterol level. The probiotics have the ability to absorb a number of cholesterol into cells. Th is research aim ed to determine the optimum incubation time of the production of arrowroot tubers with antihypercholesterolemia activity for pribiotic drink. Arrowroot tuber fermentation was carried out using L. fermentum culture with varying incubation times of 4, 8 and 12 hours. The fermentation results were evaluated organoleptically (color, aroma, texture and taste), pH, total lactic acid, total lactic acid bacteria and alcohol content. In addition, anylisis of total cholesterol levels by in vivo were also tested. The decreasing of total cholesterol level by the fermented tuber with incubation time variations of 4, 8 and 12 hours, respectively, by 15.14%, 16.96% and 28.56%. The results showed that the highest reduction percentage of total cholesterol level is 12 hours incubation time with pH value of 5.25, total lactic acid bacteria 6.4 x 10 8 CFU/mL . The total value of acquired lactic acid bacteria meets the standard for probiotic product. This indicates that the optimum incubation time for the production of probiotic arrowroot tubers as antihypercholesterolemia is 12 hours. Keywords: antihypercholesterolemic, arrowroot tuber, Lactobacillus fermentum, probiotic ABSTRAK Umbi garut merupakan salah satu tanaman potensial yang memiliki kandungan gizi tinggi sehingga dapat dijadikan sumber nutrisi bagi pertumbuhan bakteri Lactobacillus fermentum untuk pembuatan minuman probiotik. Fermentasi umbi garut dengan L. fermentum tersebut juga diketahui memiliki potensi untuk menurunkan kadar kolesterol. Hal ini disebabkan oleh kemampuan probiotik menyerap sejumlah kolesterol ke dalam sel. Penelitian ini bertujuan untuk menentukan waktu inkubasi optimum produksi bahan minuman probiotik dari umbi garut yang mempunyai aktivitas antihiperkolesterolemia. Fermentasi umbi garut dilakukan menggunakan kultur L. fermentum dengan variasi waktu inkubasi, yaitu 4, 8 dan 12 jam. Hasil fermentasi dievaluasi organoleptik (warna, aroma, tekstur dan rasa), pH, total asam laktat, total bakteri asam laktat dan kadar alkohol. Selain itu, dilakukan juga analisis penurunan kadar kolesterol total secara in vivo. Nilai persentase penurunan kadar kolesterol total oleh hasil fementasi umbi garut dengan variasi waktu inkubasi 4, 8 dan 12 jam secara berturut-turut sebesar 15,14%, 16,96% dan 28,56%. Hasil fermentasi umbi garut dengan nilai persentase penurunan kadar kolesterol total tertinggi adalah waktu inkubasi 12 jam dengan nilai pH 5,25; total asam laktat 1,56%, total bakteri asam laktat 6,4 x 10 8 CFU/mL. Nilai total bakteri asam laktat yang diperoleh memenuhi standar untuk produk  probiotik. Oleh sebab i","PeriodicalId":8988,"journal":{"name":"Biopropal Industri","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43400321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PENGARUH FERMENTASI TERHADAP TOTAL FENOLIK, AKTIVITAS PENGHAMBATAN RADIKAL DAN ANTIBAKTERI EKSTRAK TEPUNG BIJI TERATAI (Effect of Fermentation on Total Phenolic, Radical Scavenging and Antibacterial Activity of Waterlily (Nymphaea pubescens Willd.)
Biopropal Industri Pub Date : 2020-06-01 DOI: 10.36974/jbi.v11i1.5553
N. Rahmi, Nadra Khairiah, Rufida Rufida, S. Hidayati, A. Muis
{"title":"PENGARUH FERMENTASI TERHADAP TOTAL FENOLIK, AKTIVITAS PENGHAMBATAN RADIKAL DAN ANTIBAKTERI EKSTRAK TEPUNG BIJI TERATAI (Effect of Fermentation on Total Phenolic, Radical Scavenging and Antibacterial Activity of Waterlily (Nymphaea pubescens Willd.)","authors":"N. Rahmi, Nadra Khairiah, Rufida Rufida, S. Hidayati, A. Muis","doi":"10.36974/jbi.v11i1.5553","DOIUrl":"https://doi.org/10.36974/jbi.v11i1.5553","url":null,"abstract":"Waterlily seed flour is made from mature grinded waterlily seed. T he flour is commonly used as cake and local food ingredient of South Borneo. Waterlily seed s have antidiarrheal and other functional properties. This study aimed to determine the effect of fermentation on total phenolic , antiradical and antibacterial activity in waterlily seed flour. Seeds were grinded , sifted through 60 mesh screen and fermented using L. p lantarum JBSxH.6.4, BIMO-CF and spontaneous fermentation for 48 hours respectively. The fermented products were drained , dried and extracted using ethyl acetate and water solution with 70:30 ratio . The extract obtained was evaporated and determined its total phenolic by the Folin-ciocalteu method, antiradical activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and antibacterial by agar diffusion method . Furthermore, they were compared with the unfermented one . The results showed the increasing of total phenolic from 70.52±0.53 to 99.82±0.60 mg/g GAE, antiradical activity from 80.37±0.89 to 87.64±0.68% and antibacterial activity which indicate by the inhibition of E. coli, Salmonella and S. aureus. F ermentation in waterlily flour increased the total phenolic content and its biological activity, so it has the potential to be used as functional food. Keywords: antibacterial, DPPH, fermentation, total phenolic, waterlily seed ABSTRAK Tepung biji teratai dibuat dari biji teratai matang yang dihaluskan. Tepung ini biasa digunakan sebagai bahan kue dan makanan lokal khas Kalimantan Selatan. Biji teratai mempunyai khasiat antidiare dan memiliki beberapa sifat fungsional lainnya. Penelitian ini dilakukan untuk mengetahui pengaruh fermentasi terhadap total fenolik, aktivitas antiradikal dan antibakteri pada tepung biji teratai. Biji teratai dihaluskan, diayak dengan ayakan 60 mesh dan difermentasi menggunakan L. plantarum JBSxH.6.4, BIMO-CF dan fermentasi spontan, masing-masing selama 48 jam. Hasil fermentasi ditiriskan, dikeringkan dan diekstrak menggunakan pelarut etil asetat dan air dengan perbandingan 70:30. Ekstrak yang diperoleh diuapkan dan ditentukan total fenolik dengan metode Folin-ciocalteu, aktivitas penghambatan radikal menggunakan 2,2-diphenyl-1-picrylhydrazyl (DPPH) dan antibakteri dengan metode difusi agar. Selanjutnya, hasil ini dibandingkan dengan perlakuan tanpa fermentasi. Hasil pengujian menunjukkan terjadi peningkatan kandungan total fenolik 70,52±0,53 menjadi 99,82±0,60 mg/g GAE, aktivitas penghambatan radikal 80,37±0,89 menjadi 87,64±0,68% dan aktivitas antibakteri yang ditunjukkan oleh penghambatan terhadap bakteri E. coli, Salmonella dan S. aureus. Fermentasi mampu meningkatkan kandungan total fenolik dan aktivitas biologis pada tepung teratai, sehingga berpotensi digunakan sebagai bahan pangan fungsional. Kata kunci: antibakteri, biji teratai, DPPH, fermentasi, total fenolik","PeriodicalId":8988,"journal":{"name":"Biopropal Industri","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42410933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
PENGHAMBATAN AKTIVITAS ENZIM α-GLUKOSIDASE OLEH EKSTRAK KASAR ANTOSIANIN UBI JALAR UNGU SEGAR DAN PRODUK OLAHANNYA (Inhibition Activity of α-Glucosidase by Anthocyanin Crude Extract from Purple Sweet Potato and Its Products) 紫甘薯及其制品花青素粗提物对α-葡萄糖苷酶的抑制作用
Biopropal Industri Pub Date : 2019-11-07 DOI: 10.36974/jbi.v10i2.4765
S. Nurdjanah, N. Yuliana, Danita Aprisia, A. Rangga
{"title":"PENGHAMBATAN AKTIVITAS ENZIM α-GLUKOSIDASE OLEH EKSTRAK KASAR ANTOSIANIN UBI JALAR UNGU SEGAR DAN PRODUK OLAHANNYA (Inhibition Activity of α-Glucosidase by Anthocyanin Crude Extract from Purple Sweet Potato and Its Products)","authors":"S. Nurdjanah, N. Yuliana, Danita Aprisia, A. Rangga","doi":"10.36974/jbi.v10i2.4765","DOIUrl":"https://doi.org/10.36974/jbi.v10i2.4765","url":null,"abstract":"Purple sweet potato is one of the anthocyanin sources that potential to normalize blood sugar levels in diabetics because it can inhibit α-glucosidase. Since it easily damaged, processing into flour or chips is an alternative to extend the shelf life. This study aimed to determine the content of total phenol, total anthocyanin and inhibition of the α-glucosidase enzyme by crude anthocyanin extract from fresh purple sweet potato, conventional and modified flour and chips. Testing of total phenol, anthocyanin and inhibition of α-glucosidase enzyme activity were carried out using the spectrophotometric method. The results showed that total phenol, anthocyanin and inhibition of α-glucosidase enzyme activity from crude anthocyanin extracts using acid solutions in fresh purple sweet potato were respectively 291.7 mg GAEs/100g, 122.1 mg/100 g; 41.73%; sweet potato flour at 212.1 mg GAEs/100 g, 171.3 mg/100 g; 37.61%; purple sweet potato flour rich in resistant starch  of 182.5 mg GAEs/100 g, 141.1 mg/100 g; 65.59%; purple sweet potato flour partially gelatinized of 193.5 mg GAEs/100 g, 139.4 mg/100 g; 39.91%; and purple sweet potato chips of 299.5 mg GAEs/100 g, 203.6 mg/100 g, 44.73%. The ability of anthocyanin to inhibit the activity of the α-glucosidase enzyme could be increased through the retrogradation of starch and frying into chips. Keywords: α -glucosidase, anthocyanin, diabetic, purple sweet potato ABSTRAK Ubi jalar ungu merupakan salah satu sumber antosianin yang berpotensi menormalkan kadar gula darah penderita diabetes karena dapat menghambat enzim α -glukosidase. Ubi jalar ungu segar mudah mengalami kerusakan, sehingga pengolahan dalam bentuk tepung atau keripik merupakan suatu alternatif untuk memperpanjang masa simpan. Penelitan ini bertujuan untuk mengetahui kandungan total fenol, total antosianin serta penghambatan enzim α-glukosidase oleh ekstrak kasar antosianin dari ubi jalar ungu segar, tepung ubi jalar ungu konvensional dan yang termodifikasi secara fisik serta keripik ubi jalar ungu. Pengujian total fenol, antosianin dan penghambatan aktivitas enzim α -glukosidase dilakukan dengan menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa total fenol, antosianin dan  penghambatan aktivitas enzim α-glukosidase dari ekstrak kasar antosianin menggunakan larutan asam pada ubi jalar ungu segar  berturut-turut sebesar 291,7 mg GAEs/100 g, 122,1 mg/100 g; 41,73%; tepung ubi jalar sebesar 212,1 mg GAEs/100 g, 171,3 mg/100 g; 37,61%; tepung ubi jalar ungu kaya pati resisten (TP) sebesar 182,5 mg GAEs/100 g, 141,1 mg/100 g; 65,59%; tepung ubi jalar ungu tergelatinisasi parsial (TG) sebesar 193,5 mg GAEs/100 g, 139,4 mg/100 g; 39,91%; dan keripik  ubi jalar ungu (KU) sebesar 299,5 mg GAEs/100 g, 203,6 mg/100 g; 44,73% . Kemampuan antosianin dalam menghambat aktivitas enzim α-glukosidase dapat ditingkatkan melalui proses retrogradasi pati dalam ubi dan penggorengan ubi jalar ungu menjadi keripik. Kata kunci: α-glukosidas, antosi","PeriodicalId":8988,"journal":{"name":"Biopropal Industri","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47454648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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