Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity最新文献_第6页

Rapid and efficient oligonucleotide synthesis with low reagent consumption via a new synthesis column design: preparation of fluorescent dye labelled primers for application in PCR. 通过一种新的合成柱设计快速高效的低试剂消耗的寡核苷酸合成:制备荧光染料标记引物用于PCR。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

C McCollum, V Chakerian, J Kaufman, M Wenz, A Andrus

引用次数: 0

Detection of PCR products from Mycobacterium avium subspecies Paratuberculosis using oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups. 含有多个2,4-二硝基苯报告基团的寡核苷酸检测鸟分枝杆菌副结核亚种PCR产物

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

K Stevenson, C A Walker, J Grzybowski, T Brown, J M Sharp

引用次数: 0

NLPR, an agonist of AVP4-8, increases NGF gene expression in memory-impaired rat brain. NLPR是AVP4-8的激动剂，可增加记忆受损大鼠脑内NGF基因的表达。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

A W Zhou, J Guo, Y C Du

引用次数: 0

Synthesis and studies on the biophysical activity of human lung surfactant peptide SP-C and its N-terminal fragments. 人肺表面活性肽SP-C及其n端片段的合成及生物物理活性研究。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

C Schröder, A Günther, W Seeger, W Voelter

引用次数: 0

Design, synthesis and characterization of bradykinin antagonists via cyclization of the modified backbone. 通过修饰骨架环化的缓激肽拮抗剂的设计、合成和表征。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

S Reissmann, G Greiner, J Jezek, C Amberg, B Müller, L Seyfarth, L F Pineda De Castro, I Paegelow

{"title":"Design, synthesis and characterization of bradykinin antagonists via cyclization of the modified backbone.","authors":"S Reissmann, G Greiner, J Jezek, C Amberg, B Müller, L Seyfarth, L F Pineda De Castro, I Paegelow","doi":"","DOIUrl":"","url":null,"abstract":"With the aim of synthesizing cyclic antagonists of the nonapeptide hormone bradykinin with minimal side chain modification, we performed backbone to backbone and backbone to side chain cyclization. To probe and compare different strategies for this new kind of cyclization, the branched peptide bonds were formed by both reductive alkylation on the solid phase and by using preformed building units. Lactam bridges between the modified amide groups were formed by the use of the phenylalanine derivatives N(CH2COOH)Phe and N(CH2CH2NH2)Phe. The best results in the formation of the N-alkylamide bond were obtained with the coupling reagent PyBrop. The coupling rate was monitored by estimation of the N-terminal Fmoc-group. The cyclization was performed on the solid support. Unexpected difficulties resulted from the instability of the N-alkylamide bond under strong acidic conditions, as used for deprotection and for removal from the resin. We synthesized peptides with backbone to backbone cyclization between positions 2 and 5, as well as backbone to side chain cyclizations between positions 0 and 5, and between 2 and 6. The relatively high biological activities of some of the cyclic analogues support the supposed receptor-bound conformation of bradykinin antagonists with a beta-turn in the N-terminal sequence.","PeriodicalId":8980,"journal":{"name":"Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity","volume":"1 1","pages":"51-6"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20280237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of pituitary adenylate cyclase activating polypeptides (PACAPs) specific radioimmunoassay systems and distribution of PACAP-like immunoreactivity in guinea pig tissues. 垂体腺苷酸环化酶激活多肽(PACAPs)特异性放射免疫测定系统的建立及pacap样免疫反应性在豚鼠组织中的分布

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

E Ando, K Nokihara, S Naruse

引用次数: 0

Equilibrium analysis of the interaction between a synthetic peptide of influenza virus hemagglutinin and monoclonal antibodies. 流感病毒血凝素合成肽与单克隆抗体相互作用的平衡分析。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

T L McInerney, E Nice, D C Jackson

引用次数: 0

Chemical synthesis and characterisation of rat chaperonin 10: effect of chain length, ions, heat and N-terminal acetylation on unchaperoned folding into its heptameric form. 大鼠伴侣蛋白10的化学合成和表征:链长、离子、热量和n端乙酰化对无伴侣折叠成七聚体形式的影响。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

H L Ball, P Giuliani, P Lucietto, G Fossati, P Mascagni

{"title":"Chemical synthesis and characterisation of rat chaperonin 10: effect of chain length, ions, heat and N-terminal acetylation on unchaperoned folding into its heptameric form.","authors":"H L Ball, P Giuliani, P Lucietto, G Fossati, P Mascagni","doi":"","DOIUrl":"","url":null,"abstract":"Recently, the sequence of mitochondrial chaperonin 10 from Rattus norvegicus (rat cpn10), with N-terminal acetylation, has been published. Two syntheses of rat cpn10 were performed, the first using a classical carbodiimide-mediated double coupling protocol (Method A) and the second a more efficient HBTU/HOBT/single coupling procedure (Method B). The latter also involved the application of a capping procedure, using N-(2-chlorobenzyloxycarbonyloxy)succinimide [Z(2-Cl)-OSu]. The crude protein from Method A was purified using a two-step isoelectric focusing/RP-HPLC scheme and found to contain a high proportion of a deletion peptide (less Gln60). Conversely, rat cpn10 from Method B was purified to homogeneity by one-step RP-HPLC, using a reversible lipophilic chromatographic probe. The proportion of biologically active heptameric structure was directly related to the purity of the protein and attained 84% with material from Method B. The addition of Ca/Mg ions, pH 7.2, or a heating/cooling cycle increased the proportion of heptamer for less pure protein. Shorter sequences were found not to fold into heptamers, suggesting that aggregation/folding motifs are located in 1-25 and 77-101 regions of rat cpn10. The heptameric cpn10 (Method B) bound correctly to GroEL from E. coli, demonstrating that N-terminal acetylation is not necessary for its folding and binding to bacterial cpn60.","PeriodicalId":8980,"journal":{"name":"Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity","volume":"1 1","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20278936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conformational studies on beta-amyloid protein carboxy-terminal region (residues 34-42): strategic use of amide backbone protection as a structural probe. β -淀粉样蛋白羧基末端区(残基34-42)的构象研究:酰胺骨架保护作为结构探针的战略性应用。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

M Quibell, T Johnson, W G Turnell

{"title":"Conformational studies on beta-amyloid protein carboxy-terminal region (residues 34-42): strategic use of amide backbone protection as a structural probe.","authors":"M Quibell, T Johnson, W G Turnell","doi":"","DOIUrl":"","url":null,"abstract":"Analogues of beta-amyloid (32-42) peptide, containing N-(2-hydroxy-4-methoxybenzyl) (Hmb) amide backbone substitutions at various positions have been prepared using fluoren-9-ylmethoxycarbonyl (Fmoc)-polyamide based solid phase peptide synthesis. On-line N alpha-Fmoc deprotection monitoring during assembly exhibited hindered release in the native and beta A(34-42, (Hmb)Gly38) analogue syntheses. No such hindrance was observed during the synthesis of beta A(34-42, (Hmb)Gly37) nor beta A(34-42, (Hmb)Val36). However, the latter contained an exceptionally slow coupling reaction. Cleaved peptides were analysed for solubility in a variety of solvents and insoluble pellets tested for congophilic staining. X-ray analysis of Fmoc (and H-) beta A(34-42) and the corresponding (Hmb)Gly38 analogues as dimethylformamide swollen gels gave very similar structures. Secondary structure prediction and model-building of ordered arrays, compatible with our results, suggest that beta A(34-42) forms a beta-hairpin structure, with the reverse turn at Val36-Gly37-Gly38-Val39 both in solution and on the resin during synthesis.","PeriodicalId":8980,"journal":{"name":"Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity","volume":"1 1","pages":"3-12"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20278931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectroscopy and modelling of the cytoplasmic domain of the gamma-subunit of the high affinity immunoglobulin E receptor. 高亲和力免疫球蛋白E受体γ亚基细胞质结构域的光谱学和建模。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1994-01-01

G J Anderson, R R Biekofsky, M Zloh, G K Toth, I Toth, E Benedetti, W A Gibbons

{"title":"Spectroscopy and modelling of the cytoplasmic domain of the gamma-subunit of the high affinity immunoglobulin E receptor.","authors":"G J Anderson, R R Biekofsky, M Zloh, G K Toth, I Toth, E Benedetti, W A Gibbons","doi":"","DOIUrl":"","url":null,"abstract":"The high affinity receptor for IgE, Fc epsilon RI, is responsible for immediate hypersensitivity reactions. In rodents Fc epsilon RI is a tetrameric complex, alpha beta gamma 2 of non-covalently attached subunits: one IgE-binding alpha subunit with the binding site in the extracellular part of the chain, one beta-subunit and a dimer of disulphide linked gamma-subunits. Although there is an increasing evidence that the gamma-subunit chains are important signalling proteins that appear to function through a common Tyr-Leu-Tyr-Leu amino acid motif present in their cytoplasmic tails, which link the ligand binding specificity of their associated chains to signal transduction pathways, many questions related to conformation and function of this subunit remain to be answered. In the present work, the 36-residue cytoplasmic domain of the gamma-subunit has been synthesized and conformational studies by the combined use of Fourier transform infrared (FTIR), circular dichroism (CD) and nuclear magnetic resonance (NMR) have been performed. Based on the constraints found by these methods, conformational models of the cytoplasmic tail of the gamma-subunit are proposed and discussed.","PeriodicalId":8980,"journal":{"name":"Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity","volume":"1 1","pages":"31-8"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20278938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0