Biological chemistry Hoppe-Seyler最新文献_第3页

Pathogenic mechanisms induced by microbial proteases in microbial infections. 微生物蛋白酶在微生物感染中的致病机制。

Biological chemistry Hoppe-Seyler Pub Date : 1996-04-01 DOI: 10.1515/bchm3.1996.377.4.217

H Maeda, T Yamamoto

{"title":"Pathogenic mechanisms induced by microbial proteases in microbial infections.","authors":"H Maeda, T Yamamoto","doi":"10.1515/bchm3.1996.377.4.217","DOIUrl":"https://doi.org/10.1515/bchm3.1996.377.4.217","url":null,"abstract":"Most bacterial and fungal proteases excreted into infected hosts exhibit a wide range of pathogenic potentials ranging from pain, edema or even shock to translocation of bacteria from the site of infection into systemic circulation, thus resulting in septicemia. The basic mechanism or principle common to all these phenomena is explained by kinin generation, either directly from high- and/or low-molecular weight kininogens or indirectly via activation of the bradykinin generating cascade: i.e. Hageman factor-->activated Hageman factor-->prekallikrein-->kallikrein-->high-molecular weight kininogen-->bradykinin. Some bacterial proteases are also involved in activation of other host protease zymogens such as plasminogen, procollagenase (matrix metallo proteases) and proenzymes of the clotting system. Furthermore, most bacterial proteases are not only resistant to plasma protease inhibitors of the hosts, most of which belong to a group of serine protease inhibitors called serpins (serine protease inhibitors), but they also quickly inactivate serpins. Some bacterial proteases may also activate bacterial toxins thus rendering toxigenic pathogenesis. They are also capable of degrading immunoglobulins and components of the complement system and facilitate propagation of micro organisms. All in all, microbial proteases are very critical in enhancing pathogenesis of severe diseases. It is also noteworthy that bacterial cell wall components themselves, i.e. endotoxin (or lipopolysaccharide) of gram negative bacteria and teichoic/lipoteichoic acid of gram positive bacteria, are also able to activate the bradykinin generating cascade-involving activation of Hageman factor as mentioned above.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"377 4","pages":"217-26"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1996.377.4.217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19710699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 116

Synthetic peptides corresponding to alpha-lactalbumin and beta-lactoglobulin sequences with angiotensin-I-converting enzyme inhibitory activity. 合成具有抑制血管紧张素- i转换酶活性的α -乳白蛋白和β -乳球蛋白序列对应的肽。

Biological chemistry Hoppe-Seyler Pub Date : 1996-04-01 DOI: 10.1515/bchm3.1996.377.4.259

M M Mullally, H Meisel, R J FitzGerald

引用次数: 168

Putrescine active uptake system in the Trypanosomatid Crithidia fasciculata. 束状锥虫腐胺活性吸收系统的研究。

Biological chemistry Hoppe-Seyler Pub Date : 1996-04-01 DOI: 10.1515/bchm3.1996.377.4.233

M Calonge, J C Cubría, R Balaña-Fouce, D Ordóñez

{"title":"Putrescine active uptake system in the Trypanosomatid Crithidia fasciculata.","authors":"M Calonge, J C Cubría, R Balaña-Fouce, D Ordóñez","doi":"10.1515/bchm3.1996.377.4.233","DOIUrl":"https://doi.org/10.1515/bchm3.1996.377.4.233","url":null,"abstract":"Using the insect Trypanosomatid Crithidia fasciculata as a model parasite of mammalian pathogenic flagellates, i.e. Leishmania and Trypanosoma spp., we have studied the kinetic and regulatory characteristics of the polyamine uptake system. Putrescine transport was age-dependent with maximum expression values at the proliferative logarithmic phase. Putrescine transport in Crithidia fasciculata was energy-dependent and against a putrescine concentration gradient. The integrity of the membrane sulfhydryl groups was absolutely required for optimum transport rates. The specificity of this mechanism was studied in the presence of a series of different chain length aliphatic diamines, showing the high specificity for putrescine and the poor effect of this series at the highest concentration analyzed as well as the higher polyamines spermidine and spermine. Finally, the well-known inhibitor of polyamine biosynthesis, DFMO, led to an upward regulation of putrescine uptake correlating with the depletion of intracellular polyamine pool. In addition, the presence of high concentrations of putrescine in the culture medium produced a downward regulation of this system.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"377 4","pages":"233-8"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1996.377.4.233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19710700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Properties of particulate transglutaminase from Yoshida tumor cells. 吉田肿瘤细胞颗粒转谷氨酰胺酶的性质。

Biological chemistry Hoppe-Seyler Pub Date : 1996-03-01 DOI: 10.1515/bchm3.1996.377.3.167

M Signorini, L Caselli, V Lanzara, C Ferrari, P Melandri, C M Bergamini

引用次数: 7

Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy. 利用多维核磁共振光谱分析重组人甲状旁腺激素的结构。

Biological chemistry Hoppe-Seyler Pub Date : 1996-03-01 DOI: 10.1515/bchm3.1996.377.3.175

W Gronwald, D Schomburg, M P Harder, H Mayer, J Paulsen, E Wingender, V Wray

{"title":"Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy.","authors":"W Gronwald, D Schomburg, M P Harder, H Mayer, J Paulsen, E Wingender, V Wray","doi":"10.1515/bchm3.1996.377.3.175","DOIUrl":"https://doi.org/10.1515/bchm3.1996.377.3.175","url":null,"abstract":"The solution structure of human parathyroid hormone, in the form of recombinant prolyl-hPTH(1-84), has been investigated by multidimensional NMR spectroscopy under conditions (aqueous trifluoroethanol) which favour the structured-state of the protein. Spin systems were identified from 3D 1H DQF (double-quantum filtered)-COSY and TOCSY spectra and sequence-specific assignments were from 2D 1H phase-sensitive NOESY spectra. Signal overlap was resolved in a 3D-NOESY-TOCSY spectrum and assignments were confirmed with 2D NOESY-15N-HMQC (heteronuclear multiple-quantum coherence) spectra taken of a sample universally labeled with 15N. A satisfactory set of final structures was calculated from the quantitative NOE data using restrained molecular dynamics and energy minimization calculations. The N-terminus is dominated by three, well defined helices between Ser-3 to Asn-10, Ser-17 to Lys-27 and Asp-30 to Leu-37, while the most significant structural features in the C-terminus are a short, less-well defined helix between Asn-57 to Ser-62 and a series of loose turns. These two terminal units are joined by an unstructured mid-region. The molecule shows a tendency towards tertiary structure, defined by a number of long-range NOEs. A detailed RMS deviation analysis allowed the final refined structures to be classified into a limited ensemble of stable conformations that reflect the inherent flexibility of the hormone in solution.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"377 3","pages":"175-86"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1996.377.3.175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19695149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Inactivation of the very strong HCMV immediate early promoter by DNA CpG methylation in vitro. DNA CpG甲基化对HCMV强早期启动子的失活研究。

Biological chemistry Hoppe-Seyler Pub Date : 1996-03-01 DOI: 10.1515/bchm3.1996.377.3.195

S Prösch, J Stein, K Staak, C Liebenthal, H D Volk, D H Krüger

{"title":"Inactivation of the very strong HCMV immediate early promoter by DNA CpG methylation in vitro.","authors":"S Prösch, J Stein, K Staak, C Liebenthal, H D Volk, D H Krüger","doi":"10.1515/bchm3.1996.377.3.195","DOIUrl":"https://doi.org/10.1515/bchm3.1996.377.3.195","url":null,"abstract":"The influence of DNA methylation in vitro on the activity of the very strong human cytomegalovirus (HCMV) major immediate early (IE) modulator/enhancer/promoter region was investigated by transient transfection experiments of premonocytic HL-60 cells. While sequence-specific methylation of the major IE enhancer and/or modulator with the cytosine methyl-transferases FnuDII, HhaI and HaeIII had no significant effect, the promoter activity was completely repressed by methylation of the cytosine in 5'-CpG sites with the Spiroplasma methyltransferase SssI. Addition of TNF-alpha or PMA which are strong stimulators of HCMV major IE enhancer/promoter activity in premonocytic HL-60 cells had no effect on repression. Inactivation of the IE enhancer/promoter via methylation by M.SssI could be partially alleviated by co-transfection with an excess of untranscribable highly methylated DNA. These results indicate that a methyl-CpG binding factor is involved as mediator in the inhibitory effect of HCMV enhancer/promoter methylation. Taken together, the HCMV major IE enhancer/ promoter has been shown to be susceptible to transcriptional inactivation by methylation of the cytosines in CpG dinucleotides, a process that is proposed to play a modulatory role in viral latency.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"377 3","pages":"195-201"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1996.377.3.195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19696366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 116

Comparison of the substrate specificities of cAMP-dependent protein kinase from bovine heart and Ascaris suum muscle. 牛心脏和猪蛔虫肌camp依赖性蛋白激酶底物特异性的比较。

Biological chemistry Hoppe-Seyler Pub Date : 1996-03-01 DOI: 10.1515/bchm3.1996.377.3.203

T Treptau, P Piram, P F Cook, P H Rodriguez, R Hoffmann, S Jung, H P Thalhofer, B G Harris, H W Hofer

{"title":"Comparison of the substrate specificities of cAMP-dependent protein kinase from bovine heart and Ascaris suum muscle.","authors":"T Treptau, P Piram, P F Cook, P H Rodriguez, R Hoffmann, S Jung, H P Thalhofer, B G Harris, H W Hofer","doi":"10.1515/bchm3.1996.377.3.203","DOIUrl":"https://doi.org/10.1515/bchm3.1996.377.3.203","url":null,"abstract":"The catalytic subunits of cAMP-dependent protein kinases (protein kinase A) from bovine heart and Ascaris suum muscle exhibit only 48% sequence identity and show quantitative differences in substrate specificity. These differences were not obvious at the level of short synthetic substrate peptides but were distinct for some protein substrates. Phosphofructokinase from Ascaris, a physiological substrate, was a better substrate for the protein kinase from the nematode in comparison to the mammalian protein kinase due to a 10-fold lower Michaelis constant. Selective phosphorylation by the two kinases was also observed with some in vitro substrates. In addition, quantitative differences in the interactions between R- and C-subunits from Ascaris and bovine heart were observed. However, several synthetic peptides whose sequence reflected the phosphorylation site of Ascaris suum phosphofructokinase (AKGRSDS*IV), or variations of it, were phosphorylated with the same efficiency by both protein kinases. Based on the data the following are concluded: (1) In agreement with the conservation of structure in the catalytic cleft, the recognition of substrates by protein kinases from phylogenetically distant organisms exhibits similarity. (2) Non-conserved parts of the surface of the protein kinase molecule may contribute to binding of protein substrates and thus to selective recognition.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"377 3","pages":"203-9"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1996.377.3.203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19696367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Heterologous expression and characterisation of mouse brain fatty acid binding protein. 小鼠脑脂肪酸结合蛋白的异源表达及特性研究。

Biological chemistry Hoppe-Seyler Pub Date : 1996-03-01 DOI: 10.1515/bchm3.1996.377.3.211

F Schnütgen, T Börchers, T Müller, F Spener

引用次数: 20

Role of endocrine cell microvesicles in intercellular chemical transduction. 内分泌细胞微泡在细胞间化学转导中的作用。

Biological chemistry Hoppe-Seyler Pub Date : 1996-03-01 DOI: 10.1515/bchm3.1996.377.3.155

Y Moriyama, A Yamamoto, H Yamada, Y Tashiro, M Futai

引用次数: 31

Evaluation of HBV promoters for use in hepatic gene therapy. HBV启动子在肝脏基因治疗中的应用评估。

Biological chemistry Hoppe-Seyler Pub Date : 1996-03-01 DOI: 10.1515/bchm3.1996.377.3.187

P Löser, V Sandig, I Kirillova, M Strauss

引用次数: 22