Journal of pediatric biochemistry最新文献_第7页

Erythrocyte Oxidative Stress Markers in Children: A Clinical Laboratory Experience 红细胞氧化应激标志物在儿童:临床实验室经验

Journal of pediatric biochemistry Pub Date : 2015-03-01 DOI: 10.1055/s-0035-1554780

P. Hermann, R. Henneberg, A. Nascimento, M. Leonart

{"title":"Erythrocyte Oxidative Stress Markers in Children: A Clinical Laboratory Experience","authors":"P. Hermann, R. Henneberg, A. Nascimento, M. Leonart","doi":"10.1055/s-0035-1554780","DOIUrl":"https://doi.org/10.1055/s-0035-1554780","url":null,"abstract":"Abstract Because oxidative damage to erythrocytes plays an important pathophysiologic role in many diseases and there are some difficulties in obtaining reference intervals for children in many laboratory tests, this work intends to describe the determination of oxidative stress parameters and the results obtained for children. A total of 280 blood samples were obtained from children between 8 and 11 years old, without any hematological disease. Samples were analyzed for seven oxidative stress parameters, methemoglobin, reduced glutathione, TBARS, percentage of hemolysis and activity of the enzymes G6PD, superoxide dismutase, and catalase. To draw a comparison, the same parameters were analyzed in children with sickle cell disease. Because all results presented normal distribution in the Kolmogorov-Smirnov, data were expressed as 2.5 and 97.5th accumulated percentiles and the statistical analysis between male and female children was performed using Student t-test. A p value <0.05 was considered significant. Except for hemolysis, no significant difference was observed on oxidative stress parameters, in normal children, separated by sex. Except for reduced glutathione and superoxide dismutase, significant differences were observed in all parameters between normal children and those with sickle cell disease. Oxidative stress parameters can be determined in children using simple laboratory methods with small volumes of blood and the establishment of reference intervals is necessary to improve clinical decisions.","PeriodicalId":89425,"journal":{"name":"Journal of pediatric biochemistry","volume":"05 1","pages":"028 - 033"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0035-1554780","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"58070450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebrospinal Fluid Selenium Concentrations in Pediatric Patients with Neurologic Disorders 小儿神经系统疾病患者脑脊液硒浓度

Journal of pediatric biochemistry Pub Date : 2015-03-01 DOI: 10.1055/s-0035-1554782

M. Tondo, M. Casado, M. O'Callahan, I. Jordan, L. Altimira, B. Pérez-Dueñas, A. García-Alix, À. García-Cazorla, A. Ormazabal, R. Artuch

{"title":"Cerebrospinal Fluid Selenium Concentrations in Pediatric Patients with Neurologic Disorders","authors":"M. Tondo, M. Casado, M. O'Callahan, I. Jordan, L. Altimira, B. Pérez-Dueñas, A. García-Alix, À. García-Cazorla, A. Ormazabal, R. Artuch","doi":"10.1055/s-0035-1554782","DOIUrl":"https://doi.org/10.1055/s-0035-1554782","url":null,"abstract":"Abstract We aimed to study the cerebrospinal fluid (CSF) selenium (Se) status in pediatric patients with neurologic disorders and to assess the relationship between CSF Se concentrations, proteins, glutathione peroxidase (GPx) activity, and patient clinical data. We analyzed CSF Se by inductively coupled plasma-mass spectrometry (ICP-MS) in 212 patients with several neurologic conditions. Student's t-test was applied to compare CSF Se values from patients with those of controls. Single and multiple correlation studies were applied to assess the association between the different variables. Nineteen patients presented with abnormal CSF Se concentrations when compared with our reference values established for the different groups of age (group 1: 1–30 days; 1.8–4.7 µg/L; group 2: 1–36 months; 0.68–3.0 µg/L; group 3: 4–18 years; 0.73–2.13 µg/L) associated with different clinical conditions. Ten had decreased CSF Se levels and nine had increased levels. A positive correlation between CSF Se and GPx activity (r = 0.586; p <0.001), as well as with total proteins (r = 0.387; p <0.001) and with albumin (r = 0.898; p <0.001), was observed. Impaired Se concentrations affected 9% of patients with neurologic dysfunction. The positive correlation observed between CSF Se and CSF albumin values suggests that this protein markedly contributes to total Se concentrations. Also, the positive correlation observed between CSF Se values and GPx activity suggests that it may be a biomarker for antioxidant status in the CSF.","PeriodicalId":89425,"journal":{"name":"Journal of pediatric biochemistry","volume":"05 1","pages":"015 - 020"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0035-1554782","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"58070524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Serum Asymmetric Dimethylarginine Levels in Patients with Acute Rheumatic Fever 急性风湿热患者血清不对称二甲基精氨酸水平

Journal of pediatric biochemistry Pub Date : 2015-03-01 DOI: 10.1055/s-0035-1554781

A. Sert, D. Çimen, D. Arslan, E. Aypar, H. Vatansev, F. Kaya, C. Kılıçaslan, E. Aslan, O. Guvenc, F. Gun, B. Oran, D. Odabaş

{"title":"Serum Asymmetric Dimethylarginine Levels in Patients with Acute Rheumatic Fever","authors":"A. Sert, D. Çimen, D. Arslan, E. Aypar, H. Vatansev, F. Kaya, C. Kılıçaslan, E. Aslan, O. Guvenc, F. Gun, B. Oran, D. Odabaş","doi":"10.1055/s-0035-1554781","DOIUrl":"https://doi.org/10.1055/s-0035-1554781","url":null,"abstract":"Abstract Asymmetric dimethylarginine (ADMA) is an analogue of l-arginine, a naturally occurring product of metabolism found in human circulation. It is an endogenous inhibitor of nitric oxide production. Acute rheumatic fever (ARF) is a delayed immunologically mediated autoimmune sequel of throat infection by group A β-hemolytic streptococci. As serum ADMA levels have not previously been assessed in patients with ARF, we aimed to investigate ADMA levels in patients with ARF during the acute stage and after anti-inflammatory treatment and compared results with healthy control subjects. The study population consisted of 34 children with ARF (30 patients with carditis and 4 patients without carditis) and 31 healthy control subjects. Erythrocyte sedimentation rate and C-reactive protein values were significantly higher and serum ADMA values were lower, but not statistically significant in patients with ARF during the acute stage when compared with controls. Serum C-reactive protein and erythrocyte sedimentation rate values were significantly decreased in patients with ARF after the treatment when compared with baseline and ADMA levels were increased after the treatment compared with baseline, but this change was not statistically significant. Our study has demonstrated that resolution of acute inflammation in patients with ARF may lead to a mild increase in serum concentration of ADMA. Comprehensive prospective and observational studies are required to confirm our findings and to assess potential interactions between ARF and ADMA levels.","PeriodicalId":89425,"journal":{"name":"Journal of pediatric biochemistry","volume":"05 1","pages":"021 - 027"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0035-1554781","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"58070457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Measurement of Serum Osteopontin and Interleukin-17 in Asthmatic Children in Saudi Arabia 沙特阿拉伯哮喘儿童血清骨桥蛋白和白细胞介素-17的测定

Journal of pediatric biochemistry Pub Date : 2015-03-01 DOI: 10.1055/s-0035-1554779

A. Alzahrani, N. Mohammed, Humaid Al-Swat, R. Karam

引用次数: 1

Persistent Deficiency for 40% of Toddlers Who Were Vitamin D Deficient as Neonates, Which Cannot Be Assessed by Examining Symptoms of Rickets 新生儿时期维生素D缺乏的幼儿中有40%持续缺乏维生素D，这不能通过检查佝偻病的症状来评估

Journal of pediatric biochemistry Pub Date : 2015-03-01 DOI: 10.1055/s-0035-1554731

P. Hogeman, M. Hoevenaar-Blom, J. Wielders

引用次数: 1

Serum Asymmetric Dimethylarginine Levels in Patients with Acute Rheumatic Fever: Inflammation and Endothelial Dysfunction 急性风湿热患者血清不对称二甲基精氨酸水平:炎症和内皮功能障碍

Journal of pediatric biochemistry Pub Date : 2015-03-01 DOI: 10.1055/s-0035-1554730

Mahboob Alam

{"title":"Serum Asymmetric Dimethylarginine Levels in Patients with Acute Rheumatic Fever: Inflammation and Endothelial Dysfunction","authors":"Mahboob Alam","doi":"10.1055/s-0035-1554730","DOIUrl":"https://doi.org/10.1055/s-0035-1554730","url":null,"abstract":"Endothelial dysfunction is considered to be an important and early step in the initiation and progression of atherosclerosis. 1 Nitric oxide is produced by the metabolism of L -arginine via endothelial NO synthase (eNOS) to maintain appropriate endothelial function (i.e., intracellular messaging, anti-in-ﬂ ammatory, antithrombotic, and antiapoptotic effects). 1 Serum asymmetric dimethylarginine (ADMA) is an endogenous analog of L -arginine. 2 Studies have found an association between elevated ADMA levels and endothelial dysfunction in humans, which may partly be related to eNOS uncoupling that results in increased eNOS-derived superoxide production in human vessels. 2 Serum ADMA levels have been associated with coronary atherosclerosis in patients with clinical risk factors as well as in healthy individuals. 3 – 5 Plasma levels of ADMA have been associated with mortality and a worsened clinical outcome in diabetic patients with estab-lished coronary atherosclerotic disease. 6 The relationship between serum ADMA levels and acute rheumatic fever (ARF) is not well de ﬁ ned. ARF is a clinical state of heightened in ﬂ ammation driven by an autoimmune reac-tion. Sert and collogues in the accompanying article “ Serum Asymmetric Dimethylarginine Levels in Patients with Acute Rheumatic Fever ” have reported the results of their case control study exploring the relationship between markers of in ﬂ ammation (erythrocyte sedimentation rate [ESR], serum C-reactive protein [CRP] level, serum ADMA) and disease activity. 7 This study in an elegant way has demonstrated (although in a small sample) a statistical association","PeriodicalId":89425,"journal":{"name":"Journal of pediatric biochemistry","volume":"05 1","pages":"001 - 001"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0035-1554730","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"58070353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative Stress and Overview of Pediatric Disease Biomarkers 氧化应激与儿科疾病生物标志物综述

Journal of pediatric biochemistry Pub Date : 2015-03-01 DOI: 10.1055/s-0035-1554784

Ahmet Ayaz

引用次数: 3

New ideas in inborn errors of metabolism in children and infants 儿童先天性代谢异常的新观点

Journal of pediatric biochemistry Pub Date : 2014-12-01 DOI: 10.1055/s-0036-1586479

A. Gropman

引用次数: 0

Lysosomal storage disorders 溶酶体贮积症

Journal of pediatric biochemistry Pub Date : 2014-12-01 DOI: 10.1055/s-0036-1586483

P. Tanpaiboon

引用次数: 0

Metabolic muscle disorders in infants and children 婴儿和儿童的代谢肌肉紊乱

Journal of pediatric biochemistry Pub Date : 2014-12-01 DOI: 10.1055/s-0036-1586484

C. Rocha

引用次数: 0