Biomedicines最新文献

{"title":"Evaluating Cardio-Protective Molecules by Efficacy Based on Weight Reduction and HbA1c Targets.","authors":"Teodor Salmen, Valeria-Anca Pietrosel, Flaviana-Veronica Urzica, Diana-Elena Visan, Bianca-Margareta Salmen, Radu-Cristian Cimpeanu, Delia Reurean-Pintilei, Mihaela-Simona Popoviciu, Anca Pantea Stoian","doi":"10.3390/biomedicines13092306","DOIUrl":"10.3390/biomedicines13092306","url":null,"abstract":"<p><p><b>Background</b>: The effectiveness of GLP-1 RAs and SGLT-2i classes, evaluated by a HbA1c target of <7% and body weight reduction (BWR) of 5% in patients with type 2 diabetes mellitus (T2DM), represents the aim of this article. <b>Methods</b>: A retrospective analysis was conducted on consecutively admitted out-patients of a tertiary care center for diabetes mellitus (DM) treatment from Romania, where 405 enrolled patients were evaluated at baseline, 6-, and 12-month visits. <b>Results</b>: SGLT-2i were superior to GLP-1 RAs and metformin, providing higher rates of combined target achievements-22.7% at 12 months, <i>p</i> < 0.001. Both HbA1c reduction and BWR were more consistent at the 12-month visit: 6.58% for metformin, 22.68% for SGLT-2i, and 5.88% for GLP-1 Ras, as compared to baseline while the 12-month visit results were as follows: 4.79% for metformin, 5.04% for SGLT-2i and 5.88% for GLP-1 RAs as compared to the 6-month visit. Despite the fact that the HbA1c < 7% target at baseline was 38.92% for metformin, 17.64% for SGLT-2i, and 41.17% for GLP-1 RAs, BWR was achieved less, probably influenced by insulin treatment. <b>Conclusions</b>: This study shows real-world Romanian efficacy and over the time response of administering the new classes in T2DM patients when aiming for HbA1c < 7% levels and 5% BWR, with SGLT-2i outperforming metformin and GLP-1 RAs, emphasizing their growing role in the management of T2DM.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Implications of Bacteremia Caused by Non-<i>baumannii Acinetobacter</i> Compared with Those of <i>Acinetobacter baumannii</i> Bacteremia.","authors":"Jin Woong Suh, Ji Young Hong, Keun Ju Kim, Duck Jin Hong, Sun Bean Kim","doi":"10.3390/biomedicines13092304","DOIUrl":"10.3390/biomedicines13092304","url":null,"abstract":"<p><p><b>Objectives:</b> This study aimed to compare clinical characteristics, antimicrobial susceptibility, and 28-day mortality between patients with <i>Acinetobacter baumannii</i> bacteremia (ABB) and non-<i>baumannii Acinetobacter</i> bacteremia (NBAB) after rapid matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) species identification. <b>Methods:</b> We retrospectively reviewed the clinical data of adult ABB and NBAB patients over >7 years. Multivariate logistic regression was used to identify the risk factors for 28-day mortality. <b>Results:</b> Of 273 episodes of <i>Acinetobacter</i> species bacteremia, 224 (82.1%) were ABB and 49 (17.9%) were NBAB. NBA isolates were predominantly <i>A. nosocomialis</i> (49%), with smaller proportions of <i>A. bereziniae</i>, <i>A. junii</i>, <i>A. ursingii</i>, and others. The primary sites of infection in NBAB cases were the intra-abdomen, urinary tract, intravascular catheters, and lungs. While only 4.0% of <i>A. baumannii</i> isolates were susceptible to carbapenem, 87.8% of non-<i>baumannii Acinetobacter</i> isolates were susceptible. Multivariate analysis revealed that low carbapenem resistance was independently associated with NBAB. Additionally, a higher Pitt bacteremia score, septic shock, continuous renal replacement therapy, inappropriate empirical antibiotic therapy, and thrombocytopenia were independent risk factors for the 28-day mortality in patients with ABB. <b>Conclusions:</b> Although less common than ABB, NBAB cases are increasing and exhibit lower carbapenem resistance. Rapid MALDI-TOF MS identification enables timely and appropriate antibiotic treatment. The key factors driving the 28-day mortality include illness severity, septic shock, renal replacement therapy, inappropriate antibiotics, and thrombocytopenia, highlighting the need for early risk assessments and tailored management. Ongoing surveillance and species-specific strategies are essential for combating resistant <i>Acinetobacter</i> infections.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Mineral Density in HIV-Infected People-the Experience of Craiova Regional Center.","authors":"Florentina Dumitrescu, Livia Dragonu, Eugenia-Andreea Marcu, Vlad Pădureanu, Andreea Cristina Stoian, Cristiana-Luiza Rădoi-Troacă, Rodica Pădureanu, Anca Duduveche, Ilona-Andreea Georgescu, Lucian Giubelan","doi":"10.3390/biomedicines13092305","DOIUrl":"10.3390/biomedicines13092305","url":null,"abstract":"<p><p><b>Background:</b> Human Immunodeficiency Virus (HIV) is a virus that progressively impairs immune function by depleting CD4 + T-lymphocytes, ultimately leading to acquired immunodeficiency syndrome (AIDS). People living with HIV face a higher risk of developing various bone disorders, such as osteopenia, osteoporosis, and osteonecrosis. The aim of this study was to evaluate the bone mineral density (BMD) status, to determine the prevalence of osteopenia/osteoporosis and to identify the risk factors for low BMD in patients living with HIV undergoing antiretroviral treatment (ART), registered in Craiova Regional Center. <b>Methods:</b> A retrospective study was conducted between June 2024 and January 2025, including HIV-infected subjects aged over 18 years. <b>Results:</b> The study group included 106 patients. Dual-energy X-ray absorptiometry (DEXA) showed that 87 patients had low BMD, 51% having osteopenia and 31.1% having osteoporosis. We found a statistically significant correlation between low BMD and older age, higher levels HIV viremia, CD4 nadir < 200 cells/mm³, prolonged ART exposure and tenofovir disoproxil fumarate containing regimens. <b>Conclusions:</b> These findings support the inclusion of routine bone health monitoring in the standard care of patients with HIV, as well as the need for reevaluation.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Clinical Review of the Connections Between Diabetes Mellitus, Periodontal Disease, and Cardiovascular Pathologies.","authors":"Otilia Țica, Ioana Romanul, Gabriela Ciavoi, Vlad Alin Pantea, Ioana Scrobota, Lucian Șipoș, Cristian Marius Daina, Ovidiu Țica","doi":"10.3390/biomedicines13092309","DOIUrl":"10.3390/biomedicines13092309","url":null,"abstract":"<p><p><b>Background</b>: Diabetes mellitus (DM), periodontal disease (PD), and cardiovascular disease (CVD) are highly prevalent global health conditions with overlapping pathophysiological mechanisms. Emerging evidence suggests a bidirectional and synergistic relationship among them, driven by chronic inflammation, immune dysregulation, oxidative stress, and microbial dysbiosis. <b>Objective</b>: This review synthesizes current literature on the interconnectedness of DM, PD, and CVD, emphasizing shared molecular pathways, clinical implications, and opportunities for integrated management. <b>Methods</b>: A systematic review and narrative synthesis of recent clinical trials, observational studies, and multi-omics investigations was conducted to explore the mechanisms linking these three conditions. A structured literature search was performed across PubMed, Scopus, and Web of Science from database inception until 30 June 2025. Key findings were contextualized within systems biology, precision medicine, and real-world clinical strategies. <b>Results</b>: DM exacerbates periodontal inflammation and accelerates tissue destruction via hyperglycemia-induced inflammatory mediators, while periodontitis worsens glycemic control and insulin resistance. Both conditions independently elevate cardiovascular risk, and their co-occurrence significantly amplifies the incidence of adverse cardiovascular events. Shared biomarkers such as Interleukin (IL)-6, Tumor Necrosis Factor (TNF)-α, and CRP, as well as overlapping genetic and epigenetic signatures, underscore a common inflammatory axis. Periodontal therapy has demonstrated modest but meaningful benefits on glycemic control and endothelial function, while cardiometabolic therapies (e.g., statins, Glucagon-Like Peptide (GLP-1) receptor agonists, SGLT2 inhibitors) show potential to improve periodontal outcomes. Probiotics, microbiome-targeted therapies, and AI-based risk models are emerging as future tools. <b>Conclusions</b>: DM, PD, and CVD form a mutually reinforcing triad mediated by systemic inflammation and metabolic dysregulation. Integrated, multidisciplinary care models and precision health strategies are essential to address this inflammatory burden and improve long-term outcomes. Further large-scale interventional trials and mechanistic human studies are needed to establish causal links and optimize combined therapeutic approaches.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking Metabolic Disorders and Immune System Phenomena in Schizophrenia: The Role of Adipose Tissue and Inflammation.","authors":"Aleksandra Julia Oracz, Mateusz Zwierz, Maciej Naumowicz, Stefan Modzelewski, Maria Suprunowicz, Napoleon Waszkiewicz","doi":"10.3390/biomedicines13092308","DOIUrl":"10.3390/biomedicines13092308","url":null,"abstract":"<p><p>Emerging evidence highlights the role of chronic low-grade inflammation and dysregulated cytokines in both obesity and schizophrenia, suggesting overlapping immune system pathways that contribute to cognition and nervous system inflammation. Excess adipose tissue functions as an active endocrine organ, releasing pro-inflammatory mediators that may serve as potential biomarkers, while the use of antipsychotic agents in schizophrenia further modifies cytokine profiles and immune responses. A key knowledge gap lies in understanding how adipose-related inflammation modifies the severity of psychotic symptoms, cognitive deficits, and the efficacy of antipsychotic medications. This review aims to present excess adipose tissue as a potential contributor to the development of SCZ or a modifier of treatment efficacy, emphasizing the role of immune imbalance, inflammatory pathways, and metabolic dysfunction. By synthesizing current findings, we aim to present obesity not only as a frequent comorbidity in schizophrenia but also as a potential driver of neuroinflammation and disease progression. Here, we demonstrate that excess adiposity may perpetuate a vicious cycle linking metabolic dysfunction, immune activation, and psychiatric symptomatology. Situating these findings within a broader context, the review underscores the clinical need for inflammation-informed, individualized management strategies that integrate psychiatric care with metabolic monitoring. Ultimately, clarifying the shared inflammatory pathways of obesity and schizophrenia may open new avenues for biomarker development and targeted interventions.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adrenal Incidentaloma: From Silent Diagnosis to Clinical Challenge.","authors":"Alexandra Mirica, Dana-Mihaela Tilici, Diana Loreta Paun, Ana Maria Arnautu, Victor Nimigean, Sorin Paun","doi":"10.3390/biomedicines13092298","DOIUrl":"10.3390/biomedicines13092298","url":null,"abstract":"<p><p>The widespread use of advanced imaging techniques has led to a rising incidence of adrenal incidentalomas (AIs), asymptomatic adrenal masses discovered during imaging for non-adrenal-related conditions. AIs represent a diagnostic and therapeutic challenge due to their varied etiology, secretory potential, and potential for malignancy. This review aims to provide a comprehensive overview of the current knowledge on adrenal incidentalomas, focusing on their pathogenesis, diagnostic work-up, imaging features, hormonal evaluation, and evidence-based management, with a special emphasis on autonomous cortisol secretion (ACS). A thorough narrative review of the literature from the past two decades was conducted, synthesizing data from key international guidelines (ESE/ENSAT), observational studies, meta-analyses, and case series regarding the evaluation and treatment of AI. AI represents an increasingly relevant clinical condition requiring a multidisciplinary, personalized approach. Prompt endocrine and radiological evaluation is essential to identify hormonally active or potentially malignant tumors. The complexity of the natural history of AI and the evolving understanding of ACS underline the need for tailored follow-up and management strategies.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron Deficiency in Heart Failure: From ESC Guidelines to Clinical Practice at a Romanian Hospital.","authors":"Oana Sirbu, Andreea Tirnoveanu, Raluca Ecaterina Haliga, Victorita Sorodoc, Miruna Sava, Cristina Bologa, Ovidiu Rusalim Petris, Bianca Codrina Morarasu, Alexandra Diana Diaconu, Alexandr Ceasovschih, Catalina Lionte, Paula Cristina Morariu, Branco Adrian Morariu, Cristian Statescu, Radu Andy Sascau, Mariana Floria, Laurentiu Sorodoc","doi":"10.3390/biomedicines13092296","DOIUrl":"10.3390/biomedicines13092296","url":null,"abstract":"<p><p><b>Background:</b> Iron deficiency (ID) is a frequent comorbidity in heart failure (HF), associated with reduced functional capacity and poor prognosis. Although the European Society of Cardiology (ESC) guidelines recommend systematic screening and intravenous iron supplementation (IS), adherence in clinical practice remains limited. This observational study aimed to evaluate how these recommendations are implemented into practice. <b>Methods:</b> We performed a retrospective study including 4348 patients hospitalized with HF (NYHA II-IV) in a tertiary internal medicine clinic in Eastern Europe between January 2018 and September 2022. Demographic data, comorbidities, laboratory parameters, echocardiographic findings were collected from electronic medical records. IS was defined as serum ferritin < 100 ng/mL. <b>Results:</b> Among HF patients, 2547 (58.7%) were screened for ID, and 1091 (42.8%) had absolute deficiency. Only 278 patients (25.5%) received intravenous ferric carbodymaltose. Treated patients were predominantly elderly (70.1% ≥ 70 years), female (60.4%), and often had ischemic or valvular disease. Patients receiving intravenous IS showed higher NT-proBNP and troponin levels. A progressive increase in IS use was observed during the study period, with a temporary decline during the COVID-19 pandemic. <b>Conclusions:</b> Despite relatively high screening rates, only one-quarter of HF patients with confirmed ID received intravenous IS. These findings highlight persistent gaps between guidelines and clinical practice, emphasizing the need for improved awareness and implementation of ESC recommendations to optimize outcomes in HF patients with ID.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thoracic MRI in Pediatric Oncology: Feasibility and Image Quality of Post-Contrast Free-Breathing Radial 3D T1 Weighted Imaging.","authors":"Patricia Tischendorf, Marc-David Künnemann, Tobias Krähling, Jan Hendrik Lange, Walter Heindel, Laura Beck","doi":"10.3390/biomedicines13092302","DOIUrl":"10.3390/biomedicines13092302","url":null,"abstract":"<p><p><b>Objectives</b>: To compare the feasibility and image quality of a post-contrast free-breathing radial stack-of-stars 3D T1w turbo-field echo Dixon sequence (3D T1w VANE mDIXON) with a conventional cartesian breath-hold 3D T1w fast-field echo mDIXON sequence in pediatric oncology patients undergoing chest MRI. <b>Methods</b>: A total of 48 children (34 females; mean age 5.3 ± 3.7 years) underwent contrast-enhanced chest MRI, with 24 examined using the 3D T1w VANE mDIXON sequence and 24 with a conventional breath-hold 3D T1w mDIXON sequence. Image quality was independently assessed by three radiologists using a 5-point scale. Signal-to-noise ratio (SNR) was measured at two anatomical sites, a homogeneous paraspinal muscle region (SNR_muscle) and the liver apex (SNR_liver), while avoiding vessels and signal inhomogeneities. The presence of respiratory artifacts, total imaging time, and the need for general anesthesia or sedation were recorded. Interobserver agreement was determined using Fleiss's kappa (ϰ), and mean SNR values were compared between groups using an independent samples <i>t</i>-test. <b>Results</b>: The 3D T1w VANE mDIXON sequence yielded significantly higher SNR_muscle and SNR_liver (530 ± 120; 570 ± 110 vs. 370 ± 110; 400 ± 90; <i>p</i> < 0.001), improved diagnostic image quality by approximately 25%, and reduced respiratory artifacts by about 23%. Interobserver agreement was almost perfect. Importantly, the need for general anesthesia was significantly reduced using the 3D T1w VANE mDIXON (<i>p</i> < 0.001). <b>Conclusions</b>: Free-breathing 3D T1w VANE mDIXON chest MRI is a feasible and effective imaging approach for pediatric oncology patients, offering superior image quality and reducing the need for general anesthesia compared to conventional methods.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Potential Role of Adropin in Inflammatory Rheumatic Diseases-What Do We Know So Far?","authors":"Petra Simac, Marin Petric, Marijana Jankovic Danolic, Dijana Perković","doi":"10.3390/biomedicines13092300","DOIUrl":"10.3390/biomedicines13092300","url":null,"abstract":"<p><p>Adropin is a regulatory peptide hormone involved in metabolic homeostasis, cardiovascular protection, and immune modulation. Recent evidence suggests that adropin plays a role in the pathophysiology of autoimmune rheumatic diseases (ARDs) by influencing key processes such as endothelial function, oxidative stress, tissue fibrosis, and immune cell regulation. This review summarizes current knowledge on adropin's biological functions and its relevance in conditions including rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis, primary Sjögren's syndrome, osteoarthritis, psoriasis, Behçet's disease, and Kawasaki disease. We discuss how adropin interacts with various signaling pathways and highlight its potential role in macrophage polarization, regulatory T cell activity, and fibrotic remodeling. Although data remain limited and sometimes conflicting, altered adropin levels have been observed across several ARDs, suggesting potential utility as a biomarker or therapeutic target. Further research is needed to clarify its clinical significance and translational potential in immune-mediated diseases.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Th2 Suppression Through Antigen Liver Expression Using mRNA-LNP Technology.","authors":"Kazunori Arai, Hanae Toyonaga, Lei Cheng, Hirotsugu Tanaka","doi":"10.3390/biomedicines13092297","DOIUrl":"10.3390/biomedicines13092297","url":null,"abstract":"<p><p><b>Background:</b> Messenger RNA-lipid nanoparticle (mRNA-LNP) is a cutting-edge nucleic acid intracellular delivery technology. Although the clinical use of the mRNA vaccine is being actively developed, the use of mRNA-LNP technology in common diseases such as allergies is still being investigated. The purpose of this study is to test if immune response can be suppressed when an antigen is expressed in mice liver tissue with mRNA-LNP technology. <b>Methods:</b> We first designed mRNA which the ovalbumin (OVA) antigen expresses on the surface of the cells, and synthesized mRNA were encapsulated into LNP. This OVA-mRNA-LNP was evaluated with an OVA-sensitized mouse model. Splenocytes from OVA-sensitized mice were cultured with ex vivo OVA stimulation for Th2 cytokine production and Treg population analysis. Furthermore, OVA-mRNA-LNP was evaluated by both prophylactic and therapeutic administration in an OVA-induced mice airway inflammation model. <b>Results:</b> Th2 cytokines such as IL-4 and IL-5 were suppressed and the Treg population was increased in ex vivo OVA-stimulated splenocytes isolated from the OVA-mRNA-LNP administered group. Moreover, suppression of Th2 cytokines in Bronchoalveolar Lavage Fluid (BALF) from both the prophylactic and therapeutic OVA-mRNA-LNP administered cohort was observed (40-80% reduction in Th2 cytokines). <b>Conclusions:</b> The data suggests that mRNA-LNP technology, which is a safe, non-viral gene delivery system, can be an effective approach to suppress allergen-induced inflammation by expressing antigen in the liver tissue.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"13 9","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}