Bioremediation Journal最新文献

{"title":"Selection and optimization of a mixed culture for bioaugmentation based on BOD5 and COD reduction in tannery effluents","authors":"R. Perotti, P. González, C. Paisio, E. Agostini","doi":"10.1080/10889868.2021.1968335","DOIUrl":"https://doi.org/10.1080/10889868.2021.1968335","url":null,"abstract":"Abstract Most current methodologies for the treatment of tannery effluents do not succeed in bringing contaminant levels down to values that fit environmental standards in force, which means there is a need for appropriate optimization. The present work assessed effluent quality to gain further insight into their variability across time, considering both conventional parameters and those specifically related to tannery effluents, such as chromium levels. Most of the effluents analyzed exceeded the contaminant limits established by current national legislation and therefore would require treatment. To improve treatment efficiency, a mixed bacterial culture was selected on the basis of its ability to remove organic matter (OM), measured in terms of chemical oxygen demand (COD) and biochemical oxygen demand (BOD5). The four strains analyzed, belonging to the genera Bacillus, Rhodococcus and Acinetobacter, were isolated from sediments contaminated with effluents with a high organic load. The native consortium SFC 500-1, formed by Acinetobacter guillouiae SFC 500-1 A and Bacillus toyonensis SFC 500-1E, proved to be the most effective at reducing BOD5 and COD. It was selected for bioaugmentation treatment. Dissolved oxygen was found to be key for reduction, and the inoculum size required to treat effluents with different COD values was also determined. All in all, the results confirmed previous characterization of these effluents, which should always be the basis for treatment and management strategies, and also contributed to optimizing some operational parameters so that effective and safe treatment technologies may be developed.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"26 1","pages":"271 - 280"},"PeriodicalIF":2.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42798783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation of paint and textile industrial effluents by indigenous bacterial isolates","authors":"S. Adebajo, A. Ojo, P. Bankole, A. Oladotun, P. Akintokun, E. O. Ogunbiyi, Anuoluwapo Bada","doi":"10.1080/10889868.2022.2057414","DOIUrl":"https://doi.org/10.1080/10889868.2022.2057414","url":null,"abstract":"Abstract Untreated paint and textile effluents discharged from industries cause serious environmental threats to fauna and flora. This study thus, investigated the potential of different indigenous bacterial cells for the degradation of paint and textile effluents. Paint and textile effluents samples were aseptically collected from paint and textile industries. Physical, chemical, heavy metals properties and microbial load of the effluent were investigated using standard methods. Enrichment, Isolation and identification of bacterial isolates were determined using the standard microbiological methods. Screening of paint and textile effluents degraders was carried out using solid phase screening method. Degradation of paint and textile effluents were determined by Fourier-transform infrared (FTIR) analysis and spectrophotometrically. Results revealed that among the heavy metals analyzed, lead from paint effluent had highest value of 5.49 ± 1.2 mg/L. All the samples collected were turbid with highest and lowest values of 865 ± 1.3 and 514 ± 2.5 (NTU) from paint effluent samples. Paint effluent also recorded highest bacterial count of 6.85 ± 1.13 CFU/ml while paint sludge had the lowest count of 1.16 ± 0. 15 CFU/g. Forty-seven bacterial isolates were obtained from the paint and textile effluents samples. Screening of the 47 isolates showed that 21 isolates possessed the ability to grow on agar plates amended with paints and textile dyes. Out of the 21 positive isolates, 12 isolates were obtained from paint samples while nine isolates were recovered from the textile samples. Highest degradation activity was recorded by Bacillus subtilis (94.37% ±5.35) obtained from textile effluent sample while Micrococcus sp (46.55% ±8.30) from paint polluted soil sample had the lowest degradation activity. FTIR analysis showed the transformation and disappearance of peaks in the treated paint and textile effluents by Bacillus subtilis. This study revealed the ability and effectiveness of Bacillus subtilis in the removal of toxic materials in paint and textile effluents, thus could be applied for industrial use.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48303778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidation of molecular diversity and functional characterization of phenanthrene degrading consortium NS-PAH-2015-PNP-5","authors":"S. Panchal, Arpita Ghosh, Prerana Koti, Namita Singh","doi":"10.1080/10889868.2022.2059439","DOIUrl":"https://doi.org/10.1080/10889868.2022.2059439","url":null,"abstract":"Abstract Phenanthrene (PHE) is a hazardous low molecular weight PAH and widespread ecological contaminant. In the present study, the Illumina Miseq platform explored the bacterial diversity of the efficient PHE degrading consortium NS-PAH-2015-PNP-5, developed from the petrochemical contaminated soil near the Indian Oil Corporation Limited refinery, Panipat (India). Total 273 OTUs represented the species diversity identified in NS-PAH-2015-PNP-5. Nineteen major bacterial species covered approximately 99.22% of the total α-diversity of NS-PAH-2015-PNP-5. In NS-PAH-2015-PNP-5, the Shannon alpha diversity (H') and Pielou's Evenness Index (J') were calculated at 2.43 and 0.43, respectively. Moreover, an overwhelming population of phylum Proteobacteria (99.28%) and Pseudomonas spp. (32.25%) were observed in NS-PAH-2015-PNP-5. Two culturable isolates, PAH 51 and PAH 52, were isolated from the NS-PAH-2015-PNP-5, and the result of 16S rRNA analysis showed homology with Bacillus wiedmannii (96.7%) and Achromobacter pulmonis (99.24%), respectively. The results of the carbohydrate utilization test showed that NS-PAH-2015-PNP-5 was able to metabolize dextrose, L-arabinose, ONPG, esculin, citrate, and malonate. Detection of phthalic acid metabolite by the GCMS analysis inferred that NS-PAH-2015-PNP-5 bio-degraded the PHE through the protocatechuic acid pathway. During the seven-day biodegradation experiment at the optimized physicochemical conditions, NS-PAH-2015-PNP-5 degraded 62%, 70%, 90% PHE after the third, fifth, and seventh day, respectively. Graphical Abstract A foremost population of phylum Proteobacteria was identified in NS-PAH-2015-PNP-5. The culturable isolates of NS-PAH-2015-PNP-5 (PAH 51 and PAH 52) didn't utilize PHE as the carbon source. Biodegradation of PHE by the NS-PAH-2015-PNP-5 was accomplished through the protocatechuic acid pathway. Pseudomonas spp. were the most abundant species of the total α-diversity of NS-PAH-2015-PNP-5.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42731262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of ceftriaxone in soil using dioxygenase-producing genetically engineered Pseudomonas putida","authors":"G. Mardani, M. Ahankoub, Mahdiyeh Alikhani Faradonbeh, H. Raeisi Shahraki, A. Fadaei","doi":"10.1080/10889868.2022.2057412","DOIUrl":"https://doi.org/10.1080/10889868.2022.2057412","url":null,"abstract":"Abstract In this study, the degradability of the antibiotic Ceftriaxone was investigated with the help of genetically engineered Pseudomonas putida, in which the gene producing the enzyme catechol 2 and 3 dioxygenase was designed and then inserted into the pUC18 plasmid and replicated by E. coli. It was purified and extracted and transformed into Pseudomonas putida. Finally, the degradation rate of Ceftriaxone by this bacterium in spiked soil was evaluated using the HPLC measurement technique. Finally, the kinetics of Ceftriaxone degradation by genetically engineered Pseudomonas putida was investigated using zero, first, and second –order kinetic models for all factors. The results of HPLC measurement showed that the biodegradation of ceftriaxone in spiked soil was significant by genetically engineered P. putida compared to autoclaved soil inoculated by wild P. putida and normal soil with normal microbial flora (p < 0.001) and this bacterium was able to degrade ceftriaxone by 69.53% and kinetic modeling showed that the rate of removal by genetically engineered Pseudomonas putida follows the zero-degree reaction model. These findings indicate that Pseudomonas putida, which produces Catechol 2,3-dioxygenase, can be useful and practical in the biological treatment of environment from cephalosporins.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47443005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of plant growth promotion properties and impact of Microbacterium foliorum for arsenic removal in Melastoma malabathricum","authors":"Sadiya Alka, S. Shahir, Norahim Ibrahim, D. Vo, Fazilah Abd Manan","doi":"10.1080/10889868.2022.2048629","DOIUrl":"https://doi.org/10.1080/10889868.2022.2048629","url":null,"abstract":"Abstract The Microbacterium foliorum species is known for its ability to produce siderophores which, in synergy with the plant root, can effectively affect the rates of arsenate-to-arsenite mobilization, arsenic uptake, and translocation. This study investigates M. foliorum, a plant growth-promoting bacterium (PGPB), in the absorption of arsenic (As) by Melastoma malabathricum plant from contaminated soil. The current study demonstrates M. foliorum phytoremediation’s efficacy in terms of As tolerance, removal, and toxicity in the M. malabathricum plant. For an observation period of 90 days, growing plants were treated with M. foliorum in arsenic-contaminated soil. The morphological trait, pH of soil, and potential advantage induced by M. foliorum on M. malabathricum were evaluated. Plants did not display any noticeable signs of toxicity, however, the root and stem length were significantly increased in the presence of M. foliorum. The Bioconcentration Factor (BCF) was increased in plants inoculated with M. foliorum by 0.3 times, the Transfer Factor (TF) of As in the M. malabathricum plants decreased, whether in the presence or absence of PGPB. The As phytoremediation treatment with M. foliorum also enhanced uptake of As in root (by 26%) and shoot (by 22%) than in the other two (A2 and control) phytoremediation treatments. This shows that M. foliorum inoculation reduced As toxicity through substantial reduction of the adverse stress effects, increased stem and root fresh and dry weight.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"27 1","pages":"251 - 262"},"PeriodicalIF":2.0,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48617794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lab-scale bioremediation technology: Ex-situ bio-removal and biodegradation of waste cooking oil by Aspergillus flavus USM-AR1","authors":"Nurshafiqah Jasme, N. A. Md Noh, A. Yahya","doi":"10.1080/10889868.2022.2054929","DOIUrl":"https://doi.org/10.1080/10889868.2022.2054929","url":null,"abstract":"Abstract Large amounts of waste cooking oil from residential areas can cause adverse effect on the environment if not disposed properly. Fungi have a higher tolerance to hydrocarbon toxicity due to their physiology and adaptation to the environment. Consequently, they can be employed in bioremediation to reduce waste oils release to the environment. This work aims to bio-remove and biodegrade the waste cooking oil using a newly isolated strain Aspergillus flavus USM-AR1, in stirred tank reactor (STR). The strain showed remarkable removal activity of waste cooking oil. The removal efficiency of waste cooking oil reached 97% on day 3 of cultivation. This was attained when the culture was agitated at 400 rpm, maintaining the Reynold’s number of that obtained in the shake flask culture. Fortuitously, the best dispersion of waste cooking oil was also obtained at 400 rpm. The highest cell surface hydrophobicity (MATH) at 76.42% was observed at day 3 of cultivation. The maximum biosurfactant and emulsification activity occurred at day 2 of cultivation, which were 43 mm and 44%, respectively. Cell surface hydrophobicity and biosurfactant produced by strain Aspergillus flavus USM-AR1 played an important role in bio-removal of waste cooking oil, making this strain a prospective candidate for treating waste cooking oil.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"27 1","pages":"208 - 228"},"PeriodicalIF":2.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44207898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico approach for identification of polyethylene terephthalate hydrolase (PETase)-like enzymes","authors":"Poorvi Saini, Ananya Grewall, S. Hooda","doi":"10.1080/10889868.2022.2054931","DOIUrl":"https://doi.org/10.1080/10889868.2022.2054931","url":null,"abstract":"Abstract Substantial use of plastics as packaging material has become a global concern due to their limited recycling or incineration options and requires periodic degradation to avoid accumulation as a pollutant in the environment. Several chemical and physical properties such as surface area, hydrophobicity, chemical structure, molecular weight, melting temperature, crystallinity, and so on influence their biodegradation. Previously, hydrolases from Thermobifida fusca were reported to have higher degradability until Polyethylene terephthalate hydrolase (PETase) from Ideonella sakaiensis was discovered that showed better specificity for PET. The catalytic triad contains Ser160, Asp206 and His237 along with conserved serine hydrolase motif “Gly-X1-Ser-X2-Gly” having tryptophan at X1 for extending the hydrophobic surface of the active site and cysteine in the vicinity providing thermal stability via additional disulfide bonds. Here, we employed in silico screening techniques for the identification of potential plastic degrading PETases, initiating with sequence similarity search using IsPETase, scrutinizing for the presence of the conserved serine hydrolase motif containing tryptophan at X1 position and cysteine in the vicinity. Five PETases belonging to the diene-lactone hydrolase protein family from different bacterial species were identified in the initial screening, which was further confirmed by molecular docking studies indicating their capacity to bind MHET as substrate for degradation. Our study provides a reliable framework for identification and characterization of PETases involved in biodegradation of plastics which can be further explored for improving their efficiency and suitability under different conditions. This is extremely beneficial approach considering that the increasing demand for microbial enzymes due to the continued accumulation of plastics in the environment.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"27 1","pages":"311 - 323"},"PeriodicalIF":2.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48467478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test of elimination of cadmium and lead ions from water using polyurethane loaded with Cymbopogon citratus activated carbon","authors":"Wassef Al-Khatib, M. Srour, H. Bazzi, Chaden Haidar, A. Hijazi, A. E. Al-Rekaby, Wassim El Malti","doi":"10.1080/10889868.2022.2054928","DOIUrl":"https://doi.org/10.1080/10889868.2022.2054928","url":null,"abstract":"Abstract The elimination of heavy metals from industrial and agricultural wastewater became a challenging environmental topic due to their health hazards. Adsorption is one of the physicochemical techniques that proved its effectiveness in removing inorganic pollutants at low concentrations. Polymer-based composites using inorganic fillers have received increased attention due to their adsorptive properties. In addition, loaded/modified polyurethane foams have been effectively tested in removing heavy metals from polluted water. In this work, activated carbon derived from valorized Cymbopogon citratus (lemongrass) was synthesized by simple pyrolysis and activation with hydrogen peroxide and used as a filler in the polyurethane foam. The characteristics and properties of the prepared activated carbon were assessed using several methods and techniques. The cost-effective activated carbon-loaded polyurethane was synthesized in a straightforward step in the shape of pellets and used to eliminate lead (II) and cadmium (II) ions from water. It demonstrated a significant adsorption capacity even after one filtration. Furthermore, the effects of the adsorbate concentration and the number of filtrations were investigated. Highlights Cymbopogon citratus plant was valorized. Cost-effective C. citratus activated carbon-Loaded polyurethane foams were prepared to remove Cd2+ and Pb2+ from water. High removal efficiency was obtained using 150 mg/L metal ion solutions after one filtration.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"27 1","pages":"301 - 310"},"PeriodicalIF":2.0,"publicationDate":"2022-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41525056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial rhizospheric associated traits of chromate resistant bacteria for remediation of Cr (VI) contaminated soil","authors":"A. Kalsoom, R. Batool, N. Jamil","doi":"10.1080/10889868.2022.2054930","DOIUrl":"https://doi.org/10.1080/10889868.2022.2054930","url":null,"abstract":"Abstract Agricultural fields are polluted with Cr (VI) due to the excessive use of industrial effluent for irrigation purposes. The current study was focused on evaluating and comparing the impact of chromate stress on wheat growth with distilled water and industrial effluent, with and without inoculation of bacteria. All chromate-resistant bacteria (UT8, UT25, AKR2, LM3, LM8, and NY2) isolated from polluted environments proficiently resisted K2CrO4 (1500 µg/ml). Staphylococcus simulans UT8 significantly produced plant-growth-promoting compounds such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase (365.05 nmol α-ketobutyrate mg/protein/h), indole acetic acid (IAA) production (77.14 µg/ml), and hydrogen cyanide (HCN). Strain UT8 showed an increment in the germination of seeds (38.47%), and root and shoot length (95.19 and 119.29%, respectively) under chromate stress (350 µg/ml) as compared to uninoculated controls. Similarly, UT8 inoculated seedlings also exhibited remarkable augmentation in the plant height (49.18%), tiller number/plant (87.96%), and grain yield/pot (108.96%) under chromate as compared to inoculum free treatment (control). Scanning electron micrographs of S. simulans UT8 treated seedlings root exhibited enhanced colonization in the presence of Cr (VI). Chromate removal potential of strain UT8 and the competence to secrete active phytohormones make it among the agronomically potent microorganisms to enhance wheat growth in metal-polluted soils.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"27 1","pages":"189 - 207"},"PeriodicalIF":2.0,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42253812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-management potential of naturally thriving plants on the metal contaminated overburden dump of coal mines: a study from Jharkhand, India","authors":"Amudala Prathap, W. Shaikh, Kuldeep Baudhh, S. Chakraborty","doi":"10.1080/10889868.2022.2049682","DOIUrl":"https://doi.org/10.1080/10889868.2022.2049682","url":null,"abstract":"Abstract Quality of land once disturbed due to mining either by opencast or underground mines though it cannot be fully restored back, it can be reclaimed by phyto-management. However, its success depends on the selection of the plant species based on their remediation ability. Here, an attempt was made to select native plant species which are dominant in a mining area of Kuju and Charhi, Jharkhand, India and have good metal accumulating capacity from coal mine overburden (OB) dumps. First, vegetation community study was performed to identify the dominant herb and shrub species through quadrat method. Seven dominant plant species were selected from the OB dumps and analyzed for metal uptake. Bioconcentration factor (BCF) and translocation factor (TF) of Cd, Ni, As, Fe, Pb, Mn, Zn and Se were calculated to understand the pattern of bioaccumulation and translocation into the various plant parts, respectively. Four plant species viz. Cynodon dactylon, Eulaliopsis binata, Croton oblongifolius, Lantana indica were found to be abundant in the area and efficient accumulators of metals from the soil. The order of uptake for the various metals in mg/kg of dry weight by the abundant plant species were Fe: 1191 > Mn: 441 > Zn: 232 > As: 12.5 > Ni: 10.91 > Pb: 8.6 > Se: 0.7 > Cd: 0.15. These species can thus be further utilized for phytoremediation of the degraded OB dumps and similar studies can help in other OB dump reclamation in other geographical and climatic regimes.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"27 1","pages":"290 - 300"},"PeriodicalIF":2.0,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42396124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}