Diabetic nephropathy : DN最新文献_第2页

The role of vitamin D receptor agonist on podocyte injury induced by high glucose 维生素D受体激动剂在高糖足细胞损伤中的作用

Diabetic nephropathy : DN Pub Date : 2022-01-01 DOI: 10.2478/dine-2022-0010

Zhiyuan Wang, Jianfeng Guo

{"title":"The role of vitamin D receptor agonist on podocyte injury induced by high glucose","authors":"Zhiyuan Wang, Jianfeng Guo","doi":"10.2478/dine-2022-0010","DOIUrl":"https://doi.org/10.2478/dine-2022-0010","url":null,"abstract":"Abstract Background The effects of vitamin D receptor (VDR) agonist paricalcitol on the podocyte injury induced by high glucose (HG) were investigated in conditioned immortalized mouse podocytes (MPC-5). Methods (1) Grouped according to different glucose concentrations: normal group (NG): 5.6 mmol/L glucose; HG stimulation group: 25 mmol/L glucose (25HG); high osmotic control group (NG+M): 5.6 mmol/L glucose + 19.4 mmol/L D-mannitol. The expression levels of VDR, podocyte marker proteins podocin, nephrin and mesenchymal marker proteins α-smooth muscle actin (α-SMA), matrix metalloproteinases (MMP9) in MPC-5 were measured, respectively. (2) Effect of VDR agonist-paricalcitol on podocyte epithelial-mesenchymal transition (EMT) induced by HG: cultured podocytes are divided into NG group, NG with dimethylsulfoxide (DMSO) group (NG+D), NG with paricalcitol (0.1 μmol/L) group (NG+P), HG group, HG with DMSO group (HG+D), and HG with paricalcitol (0.1 μmol/L) group (HG+P). The expression levels of VDR, podocyte marker proteins, marker proteins of mesenchymal cells, and the albumin flow in each group were then detected. Results (1) Under HG conditions, the expressions of VDR, podocin, and nephrin were decreased, while the expressions of α-SMA and MMP9 were increased (all P < 0.05). After administration of paricalcitol, the levels of VDR, podocin, and nephrin were increased, while the expressions of α-SMA and MMP9 were decreased compared with the control groups (all P < 0.05). (2) The results of albumin flow showed that the albumin flow of podocytes increased under the condition of HG, while it decreased after the treatment of paricalcitol. Conclusion The podocyte injury induced by HG could be partly rescued by Paricalcitol.","PeriodicalId":89356,"journal":{"name":"Diabetic nephropathy : DN","volume":"26 1","pages":"18 - 24"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86888000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The application effect of the trans-theoretical model of behavior change in diabetic kidney disease patients treated with maintenance hemodialysis 行为改变跨理论模型在糖尿病肾病维持性血液透析患者中的应用效果

Diabetic nephropathy : DN Pub Date : 2022-01-01 DOI: 10.2478/dine-2022-0013

Yuanyuan Wang, Xiaodan Liu, Pengzhi Wan, Miao-Lin Wu, Ye Geng, L. Yao

{"title":"The application effect of the trans-theoretical model of behavior change in diabetic kidney disease patients treated with maintenance hemodialysis","authors":"Yuanyuan Wang, Xiaodan Liu, Pengzhi Wan, Miao-Lin Wu, Ye Geng, L. Yao","doi":"10.2478/dine-2022-0013","DOIUrl":"https://doi.org/10.2478/dine-2022-0013","url":null,"abstract":"Abstract Objectives The application effect of the trans-theoretical model (TTM) of behavior change in diabetic kidney disease (DKD) patients treated with maintenance hemodialysis (MHD) was discussed. Methods According to the random number table method, 80 patients with DKD on MHD were divided into control group and observation group, 40 patients in each group. Results After the intervention for 6 months, the self-rating depression scale (SDS) and self-rating anxiety scale (SAS) scores of patients in the observation group were all lower than those before the intervention and those in the control group (P < 0.05); the compliance of patients to fluid intake in the observation group was higher than that in the control group while the total incidence rate of associated acute complications of the former was lower than that of the latter (P < 0.05). The fasting blood glucose (FBG) and 2-h postprandial blood glucose (2hPBG) indexes of the observation group were lower than those of the control group (P < 0.05), and the laboratory test indexes of serum creatinine (SCr), blood urea nitrogen (BUN), glomerular filtration rate (GFR) were lower than those before the intervention and lower than those of the control group (P < 0.05). Conclusions The nursing combined with TTM of behavior change can effectively strengthen the compliance of DKD patients subjected to MHD to fluid intake, reduce patients’ negative moods (such as depression and anxiety), and lower the incidence rate of complications, and improve the life quality.","PeriodicalId":89356,"journal":{"name":"Diabetic nephropathy : DN","volume":"26 1","pages":"24 - 30"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91188366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation analysis between Tervaert glomerular classification and clinical indicators in patients with type 2 diabetic nephropathy 2型糖尿病肾病Tervaert肾小球分型与临床指标的相关性分析

Diabetic nephropathy : DN Pub Date : 2022-01-01 DOI: 10.2478/dine-2022-0014

Jing Zhao, Yonggui Wu

{"title":"Correlation analysis between Tervaert glomerular classification and clinical indicators in patients with type 2 diabetic nephropathy","authors":"Jing Zhao, Yonggui Wu","doi":"10.2478/dine-2022-0014","DOIUrl":"https://doi.org/10.2478/dine-2022-0014","url":null,"abstract":"Abstract Purpose To investigate the correlation analysis of Tervaert glomerular classification and clinical indicators in patients with type 2 diabetic nephropathy (DN). Methods We collected the renal sections of patients with DN and used immunoglobulin G (IgG), albumin (Alb), PAS, and MASSON staining to observe the extent of glomerular lesions. We simultaneously collected their clinical data for statistics and conducted correlation analysis with Tervaert glomerular classification. Moreover, we collected patients’ urine protein components data and conducted a correlation analysis with Tervaert classification. Results Tervaert classification reflects glomerular changes and was positively related to proteinuria, creatinine (Cr), and blood urea nitrogen but was negatively related to estimated glomerular filtration rate (eGFR). Tervaert classification was also positively related to total protein (TP), transferrin (TRF), retinol binding protein (RBP), N-acetyl-β-D aminoglucosidase (NAG), Alb, cystatin C (Cyc), IgG, β2-microglobulin (β2-MG), α1-microglobulin (α1-MG), Alb/Cr, and TP/Cr in urine. Conclusion Our study reveals that clinical indicators could well reflect glomerular lesions and has great value for the diagnosis and treatment of early DN.","PeriodicalId":89356,"journal":{"name":"Diabetic nephropathy : DN","volume":"112 1","pages":"32 - 39"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82497200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGF-β signaling in diabetic nephropathy: An update TGF-β信号在糖尿病肾病中的作用:最新进展

Diabetic nephropathy : DN Pub Date : 2022-01-01 DOI: 10.2478/dine-2022-0011

Li Wang, Honglian Wang, H. Lan

{"title":"TGF-β signaling in diabetic nephropathy: An update","authors":"Li Wang, Honglian Wang, H. Lan","doi":"10.2478/dine-2022-0011","DOIUrl":"https://doi.org/10.2478/dine-2022-0011","url":null,"abstract":"Abstract Diabetic nephropathy (DN) is a common complication in patients with diabetes and the leading cause of end-stage renal disease. Accumulating evidence shows that transforming growth factor beta-1 (TGF-β1) is a key mediator in the pathogenesis of DN. TGF-β1 binds to its receptors to activate canonical and noncanonical downstream signaling pathways to exert its biological activities. Among them, canonical Smad signaling is the major pathway responsible for the development of DN. In addition to TGF-β1, many stress molecules, such as advanced glycation end products (AGEs), angiotensin II (Ang II), and C-reactive protein (CRP), can also activate Mothers against decapentaplegic homologs (Smads) via the extracellular signal-regulated kinase (ERK)/p38 mitogen-activated protein kinase (MAPK) cross talk mechanism. Furthermore, TGF-β/Smad signaling can also cross talk with nuclear factor kappa B (NF-κB) signaling to regulate renal inflammation via the induction of IκBα by Smad7. In the context of renal fibrosis, Smad3 is pathogenic, while Smad2 and Smad7 are protective. TGF-β signaling also upregulates the pathogenic microRNAs (miRNAs) (namely, miR-21, miR-192, and miR-377) and long noncoding RNAs (lncRNAs) (namely, Erbb4-IR (intron region, IR), LncRNA9884, and Arid2-IR) but downregulates the protective miRNAs (namely, miR-29a/b and miR-200a) to mediate DN. Thus, targeting TGF-β signaling, either by blocking its ligand, its receptor (i.e., TGF-β receptor-2 [TGFBR2]), Smad3, and downstream miRNAs/lncRNAs or by overexpressing Smad7, has been shown to improve DN. In addition, pharmaceutically targeting TGF-β signaling using chemical inhibitors and traditional Chinese medicine (TCM), including Tangshen formula, Chaihuang-Yishen granule, and herbal extracts (berberine, asiatic acid, and naringenin), also shows renoprotective effect in diabetes. In summary, TGF-β signaling is a critical pathway leading to DN and may be a therapeutic target for combating DN.","PeriodicalId":89356,"journal":{"name":"Diabetic nephropathy : DN","volume":"6 1","pages":"7 - 16"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73264318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging role of macrophages in diabetic nephropathy 巨噬细胞在糖尿病肾病中的新作用

Diabetic nephropathy : DN Pub Date : 2021-12-01 DOI: 10.2478/dine-2022-0008

Huiyao Lan

{"title":"Emerging role of macrophages in diabetic nephropathy","authors":"Huiyao Lan","doi":"10.2478/dine-2022-0008","DOIUrl":"https://doi.org/10.2478/dine-2022-0008","url":null,"abstract":"Abstract Increasing evidence shows that diabetic nephropathy is associated with immune disorder. Macrophages are a key immune cell infiltrating the kidney in both patients and experimental animal models of diabetes, and correlate with progressive renal injury under diabetic conditions. Blockade of renal macrophage infiltration by either genetic deletion or pharmacological inhibition has been shown to improve diabetic renal injury, revealing a pathogenic role of macrophages in diabetic nephropathy. Further, studies identify that M1 macrophages are a key player responsible for diabetic renal injury by triggering renal inflammation, while M2 macrophages are highly heterogenous, and may play diverse roles in either initiating the renal repairing process if renal inflammation is resolved, or promoting progressive renal fibrosis via a macrophage-to-myofibroblast transition (MMT) process if renal inflammation is ongoing. Macrophages may also interact with intrinsic kidney cells to mediate renal inflammation or fibrosis directly or indirectly by producing a variety of proinflammatory cytokines/chemokines and growth factors, or by macrophage-derived exosomes. In summary, macrophages are immunologically important in the pathogenesis of diabetic kidney disease and may play a driving role in the progression of diabetic nephropathy. Targeting macrophages may thus be considered as a novel therapy for combatting diabetic nephropathy.","PeriodicalId":89356,"journal":{"name":"Diabetic nephropathy : DN","volume":"18 1","pages":"93 - 96"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84634976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diabetic nephropathy with crescent: A case report 糖尿病肾病伴新月1例

Diabetic nephropathy : DN Pub Date : 2021-12-01 DOI: 10.2478/dine-2022-0007

F. Tian, Zhengyong Li, D. Gao, Dongwei Liu

引用次数: 0

Pyridoxamine alleviates high glucose induced fibrosis in renal tubular epithelial cell by inhibiting the activity of TGF-β1/Smad3 signaling pathway 吡哆沙明通过抑制TGF-β1/Smad3信号通路活性减轻高糖诱导的肾小管上皮细胞纤维化

Diabetic nephropathy : DN Pub Date : 2021-12-01 DOI: 10.2478/dine-2022-0005

Ziqiang Wang, Ying Li, Ying Wang, K. Zhao, Yanqing Chi, Baoxing Wang

{"title":"Pyridoxamine alleviates high glucose induced fibrosis in renal tubular epithelial cell by inhibiting the activity of TGF-β1/Smad3 signaling pathway","authors":"Ziqiang Wang, Ying Li, Ying Wang, K. Zhao, Yanqing Chi, Baoxing Wang","doi":"10.2478/dine-2022-0005","DOIUrl":"https://doi.org/10.2478/dine-2022-0005","url":null,"abstract":"Abstract Background Renal fibrosis is one of the main characteristics of diabetic nephropathy. TGF-β1/Smad3 pathway is expected to reveal the pathogenesis of renal fibrosis in diabetic nephropathy (DN). Pyridoxamine (PM), a natural form of vitamin B6, is a powerful inhibitor of advanced glycation end products (AGEs). PM plays an anti-apoptotic, anti-oxidative stress, and fibrosis role in DN. The purpose of this study was to assess whether PM has a protective effect in renal tubular epithelial and to investigate its possible mechanism. Methods The effects of PM were investigated in HK-2 cells induced by high glucose. HK-2 cells were administered with PM at a dose of 1 mmol/L. Western blot and Realtime PCR were used to detect the expression levels of renal fibrosis related proteins. The possible mechanism of PM was examined by expression of transforming growth factor-β1 (TGF-β1)/Smad3 pathway. Results PM could reduce the expression of Fibronectin (FN) and α-smooth muscle actin (α-SMA) induced by high glucose. PM could also affect the activity of TGF-β1/Smad3 pathway in HK-2 cells. FN and α-SMA were up-regulated by overexpression of Smad3 for 48 h. After adding PM, the levels of FN and α-SMA are significantly decreased. Conclusion Our findings indicate that PM showed a protective effect in HK-2 cells through the inhibition of TGF-β1/Smad3 pathway.","PeriodicalId":89356,"journal":{"name":"Diabetic nephropathy : DN","volume":"16 1","pages":"109 - 113"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82606316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium-glucose cotransporter 2 (SGLT2) inhibitors for the prevention and treatment of diabetic kidney disease: A network meta-analysis of randomized controlled trials 钠-葡萄糖共转运蛋白2 (SGLT2)抑制剂预防和治疗糖尿病肾病:随机对照试验的网络荟萃分析

Diabetic nephropathy : DN Pub Date : 2021-09-01 DOI: 10.2478/dine-2022-0012

L. Fang, J. Duan, Jinsong Geng, Zhangsuo Liu, Jiancheng Dong

{"title":"Sodium-glucose cotransporter 2 (SGLT2) inhibitors for the prevention and treatment of diabetic kidney disease: A network meta-analysis of randomized controlled trials","authors":"L. Fang, J. Duan, Jinsong Geng, Zhangsuo Liu, Jiancheng Dong","doi":"10.2478/dine-2022-0012","DOIUrl":"https://doi.org/10.2478/dine-2022-0012","url":null,"abstract":"Abstract Objectives To evaluate and compare the effectiveness and safety of sodium-glucose cotransporter 2 (SGLT2) inhibitors for the prevention and treatment of diabetic kidney disease (DKD). Methods We searched articles in PubMed, Embase, and the Cochrane Central Register of Controlled Trials, which are published from 2010 to 2021, to identify randomized controlled trials (RCTs) by comparing SGLT2 inhibitors with placebo. A network meta-analysis (NMA) was conducted within a frequency framework using a random-effects model. Results We included 16 studies involving 51,925 patients in the analysis. Only empagliflozin significantly lowered urine albumin-to-creatinine ratio (UACR) than a placebo (mean differences [MD]: −83.01, 95% confidence intervals [CI]: −117.74 to −48.27). With regard to the composite kidney outcomes, canagliflozin (relative risk [RR] = 0.74, 95% CI: 0.69–0.80), dapagliflozin (RR = 0.76, 95% CI: 0.68–0.85), empagliflozin (RR = 0.69, 95% CI: 0.63–0.76), and ertugliflozin (RR = 0.82, 95% CI: 0.68–0.99) were significantly associated with a lower risk than placebo. Conclusions The UACR-lowering effects of empagliflozin were greater than most other SGLT2 inhibitors. There were few clinically significant differences in the renal protective effects among these drugs.","PeriodicalId":89356,"journal":{"name":"Diabetic nephropathy : DN","volume":"1 1","pages":"114 - 124"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79938738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Diabetic nephropathy patient with heavy proteinuria: A case report 糖尿病肾病伴重度蛋白尿1例

Diabetic nephropathy : DN Pub Date : 2021-09-01 DOI: 10.2478/dine-2022-0002

Pei Yu, Manman Lu, Dongwei Liu, D. Gao

引用次数: 0

Diabetic kidney disease, a potentially serious issue resulting from collision of the coronavirus disease 2019 and diabetes global pandemics 糖尿病肾病，这是2019年冠状病毒病和糖尿病全球大流行碰撞造成的潜在严重问题

Diabetic nephropathy : DN Pub Date : 2021-09-01 DOI: 10.2478/dine-2022-0003

Z. Chai, P. Zimmet, M. Cooper

引用次数: 0