Chimerism最新文献_第8页

Tolerance induction after organ transplantation, "delayed tolerance," via the mixed chimerism approach: planting flowers in a battle field. 器官移植后的耐受性诱导，“延迟耐受性”，通过混合嵌合的方式:在战场上种花。

Chimerism Pub Date : 2012-01-01 DOI: 10.4161/chim.20096

Yohei Yamada, Gilles Benichou, A Benedict Cosimi, Tatsuo Kawai

{"title":"Tolerance induction after organ transplantation, \"delayed tolerance,\" via the mixed chimerism approach: planting flowers in a battle field.","authors":"Yohei Yamada, Gilles Benichou, A Benedict Cosimi, Tatsuo Kawai","doi":"10.4161/chim.20096","DOIUrl":"https://doi.org/10.4161/chim.20096","url":null,"abstract":"We have previously reported that peri-transplant conditioning leads to successful induction of renal allograft tolerance via the mixed chimerism approach in nonhuman primates (NHP) and humans. However, this strategy requires treatments beginning six days prior to transplantation, which limits its relevance only to living donor transplant recipients. To extend the clinical applicability of this approach, we developed a novel regimen “delayed tolerance,” with which the recipient initially undergoes organ transplantation with conventional immunosuppression, followed by conditioning and donor bone marrow transplantation (DBMT) at a later date. This approach might be likened to “planting flowers in a battle field.” That is, the recipient’s immunologic environment after organ transplantation is like a battlefield filled with hostile innate and adaptive immune-responses directed against donor antigeneic specificities. Implanting fragile donor hematopoietic progenitors into this environment and encouraging them to bloom in this vicious field requires special treatments. In our NHP studies recently published in The American Journal of Transplantation, we showed that such “delayed tolerance,” in fact, can be induced in NHP through the mixed chimerism approach, if specific modifications to overcome/avoid donor-specific memory T cell responses are provided. These modifications include adequate depletion of CD8 memory T cells and timing of donor bone marrow administration to minimize levels of pro-inflammatory cytokines. This article addendum will provide a short summary of the original paper with our additional insights and interpretations.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"3 1","pages":"24-8"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.20096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30684179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Fetal-maternal interface: a chronicle of allogeneic coexistence. 胎母界面:异基因共存的编年史。

Chimerism Pub Date : 2012-01-01 DOI: 10.4161/chim.19439

Josep-Maria Pujal, Santiago Roura, Ana M Muñoz-Marmol, Jose-Luis Mate, Antoni Bayes-Genis

引用次数: 5

Immigration control in the vertebrate body with special reference to chimerism. 脊椎动物体内的移入控制，特别涉及嵌合。

Chimerism Pub Date : 2012-01-01 DOI: 10.4161/chim.20113

Anthony J S Davies

引用次数: 10

Utility of saliva and hair follicles in donor selection for hematopoietic stem cell transplantation and chimerism monitoring. 唾液和毛囊在造血干细胞移植供体选择和嵌合监测中的应用。

Chimerism Pub Date : 2012-01-01 DOI: 10.4161/chim.19395

Gurvinder Kaur, Neeraj Kumar, Ramya Nandakumar, Chowphi C Rapthap, Gaurav Sharma, Shekhar Neolia, Heena Kumra, Prateek Mahalwar, Abhinav Garg, Sunil Kumar, Jasmeet Kaur, Mrinali Hakim, Lalit Kumar, Narinder K Mehra

{"title":"Utility of saliva and hair follicles in donor selection for hematopoietic stem cell transplantation and chimerism monitoring.","authors":"Gurvinder Kaur, Neeraj Kumar, Ramya Nandakumar, Chowphi C Rapthap, Gaurav Sharma, Shekhar Neolia, Heena Kumra, Prateek Mahalwar, Abhinav Garg, Sunil Kumar, Jasmeet Kaur, Mrinali Hakim, Lalit Kumar, Narinder K Mehra","doi":"10.4161/chim.19395","DOIUrl":"https://doi.org/10.4161/chim.19395","url":null,"abstract":"Selection of an HLA identical donor is a critical pre-requisite for successful hematopoietic stem cell transplantation (HSCT). Most transplant centers utilize blood as the most common source of DNA for HLA testing. However, obtaining blood through phlebotomy is often challenging in patients with conditions like severe leucopenia or hemophilia, pediatric and elderly patients. We have used a simple in-house protocol and shown that HLA genotypes obtained on DNA extracted from saliva or hair are concordant with blood and hence can be used for selection of donors for HSCT or organ transplantation. Similarly, for post-HSCT chimerism monitoring, non-availability of pre-transplant DNA samples poses a major limitation of reference STR fingerprints. This study shows that DNA obtained post-HSCT from hair follicles can be used to generate pre-transplant patient specific fingerprints while the STR profiles obtained in saliva samples cannot as these display a mixed state of chimerism.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"3 1","pages":"9-17"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.19395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30684176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Donor-derived peripheral mononuclear cell DNA is associated with stable kidney allograft function: a randomized controlled trial. 供体来源的外周单核细胞DNA与稳定的同种异体肾移植功能相关:一项随机对照试验。

Chimerism Pub Date : 2011-10-01 DOI: 10.4161/chim.2.4.19095

Ghasem Solgi, Joannis Mytilineos, Vijayakrishna Gadi, Biswajit Paul, Gholamreza Pourmand, Abdolrasoul Mehrsai, Behrouz Nikbin, Ali Akbar Amirzargar

{"title":"Donor-derived peripheral mononuclear cell DNA is associated with stable kidney allograft function: a randomized controlled trial.","authors":"Ghasem Solgi, Joannis Mytilineos, Vijayakrishna Gadi, Biswajit Paul, Gholamreza Pourmand, Abdolrasoul Mehrsai, Behrouz Nikbin, Ali Akbar Amirzargar","doi":"10.4161/chim.2.4.19095","DOIUrl":"https://doi.org/10.4161/chim.2.4.19095","url":null,"abstract":"A large body of literature has documented an inconsistent relationship of peripheral donor cell chimerism with alloimmune tolerance following kidney transplantation. We revisit this association with assays capable of quantifying cellular microchimerism with 150–1500-fold greater sensitivity than previously utilized allo-antibody based flow cytometric approaches. Forty renal transplant patients, 20 with concurrent donor bone marrow infusion (DBMI) and 20 control participants without infusion were prospectively monitored for peripheral blood microchimerism using donor polymorphism-specific quantitative real-time PCR. Thirty-eight patients were evaluated for microchimerism, 19 in each group. The frequency of testing positive for (95% vs. 58%, p = 0.02) and mean concentrations of microchimerism (115 ± 66 vs. 13 ± 3 donor genomes/million recipient genomes, p = 0.007), respectively, were higher in infused patients compared with controls. Thirty-one patients maintained stable graft function; 17 in the DBMI group vs. 14 in controls. Patients with stable graft function in the DBMI group compared with control patients harbored microchimerism more frequently (94 vs. 50%, p = 0.01) and at higher concentrations (123 ± 67 vs. 11 ± 4, p = 0.007), respectively. Significant correlation between dose of infused cells and microchimerism levels was found post-transplant (p = 0.01). Using very sensitive assays, our findings demonstrate associations between the presence and quantity of microchimerism with stable graft function in infused patients.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"2 4","pages":"102-10"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.2.4.19095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30579843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Th2 alloimmunity counteracts Th17-type response in the neonatal establishment of lymphoid chimerism. Th2同种免疫在新生儿淋巴嵌合的建立中抵消th17型反应。

Chimerism Pub Date : 2011-10-01 DOI: 10.4161/chim.2.4.19162

Isabelle Debock, Véronique Flamand

引用次数: 2

Host stem cells repopulate liver allografts: reverse chimerism. 宿主干细胞重新填充同种异体肝脏移植物:反向嵌合。

Chimerism Pub Date : 2011-10-01 DOI: 10.4161/chim.2.4.19177

Zhaoli Sun, George Melville Williams

引用次数: 5

HLA-targeted cell sorting of microchimeric cells opens the way to phenotypical and functional characterization. hla靶向细胞分选为嵌合细胞的表型和功能表征开辟了道路。

Chimerism Pub Date : 2011-10-01 DOI: 10.4161/chim.2.4.19133

Michael Eikmans, Frans H J Claas

引用次数: 3

Mixed chimerism and split tolerance: mechanisms and clinical correlations. 混合嵌合和分裂耐受:机制和临床相关性。

Chimerism Pub Date : 2011-10-01 DOI: 10.4161/chim.2.4.19017

David P Al-Adra, Colin C Anderson

{"title":"Mixed chimerism and split tolerance: mechanisms and clinical correlations.","authors":"David P Al-Adra, Colin C Anderson","doi":"10.4161/chim.2.4.19017","DOIUrl":"https://doi.org/10.4161/chim.2.4.19017","url":null,"abstract":"Establishing hematopoietic mixed chimerism can lead to donor-specific tolerance to transplanted organs and may eliminate the need for long-term immunosuppressive therapy, while also preventing chronic rejection. In this review, we discuss central and peripheral mechanisms of chimerism induced tolerance. However, even in the long-lasting presence of a donor organ or donor hematopoietic cells, some allogeneic tissues from the same donor can be rejected; a phenomenon known as split tolerance. With the current goal of creating mixed chimeras using clinically feasible amounts of donor bone marrow and with minimal conditioning, split tolerance may become more prevalent and its mechanisms need to be explored. Some predisposing factors that may increase the likelihood of split tolerance are immunogenicity of the graft, certain donor-recipient combinations, prior sensitization, location and type of graft and minimal conditioning chimerism induction protocols. Additionally, split tolerance may occur due to a differential susceptibility of various types of tissues to rejection. The mechanisms involved in a tissue's differential susceptibility to rejection include the presence of polymorphic tissue-specific antigens and variable sensitivity to indirect pathway effector mechanisms. Finally, we review the clinical attempts at allograft tolerance through the induction of chimerism; studies that are revealing the complex relationship between chimerism and tolerance. This relationship often displays split tolerance, and further research into its mechanisms is warranted.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"2 4","pages":"89-101"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.2.4.19017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30578310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Layers of regulation in induction of mixed chimerism by anti-CD40L. 抗 CD40L 诱导混合嵌合体过程中的多层调节。

Chimerism Pub Date : 2011-10-01 DOI: 10.4161/chim.2.4.19053

Carrie L Lucas, Megan Sykes

引用次数: 0