Chimerism最新文献_第2页

No irradiation required: The future of humanized immune system modeling in murine hosts. 无需辐照:小鼠宿主人源化免疫系统建模的未来。

Chimerism Pub Date : 2015-04-03 Epub Date: 2016-05-12 DOI: 10.1080/19381956.2016.1162360

Brian E McIntosh, Matthew E Brown

引用次数: 11

Positive effect of fetal cell microchimerism on tumor presentation and outcome in papillary thyroid cancer 胎儿细胞微嵌合对甲状腺乳头状癌的肿瘤表现和预后的积极影响

Chimerism Pub Date : 2014-10-02 DOI: 10.1080/19381956.2015.1107254

V. Cirello, L. Fugazzola

引用次数: 3

Microchimerism and regulation in living related kidney transplant families 活体肾移植家族的微嵌合及其调控

Chimerism Pub Date : 2014-10-02 DOI: 10.1080/19381956.2015.1111974

W. Haynes, E. Jankowska‐Gan, L. Haynes, W. Burlingham

{"title":"Microchimerism and regulation in living related kidney transplant families","authors":"W. Haynes, E. Jankowska‐Gan, L. Haynes, W. Burlingham","doi":"10.1080/19381956.2015.1111974","DOIUrl":"https://doi.org/10.1080/19381956.2015.1111974","url":null,"abstract":"Long-term harmful effects of immunosuppressive drugs and chronic rejection are a persistent impetus to establish methods to induce immunological tolerance to allografts. PCR-based studies have found evidence that humans and other placental mammals can have prolonged extremely low levels of maternal cells as well as other non-self cells, referred to as microchimerism. The persistence of these cells suggests a mechanism for the maintenance of the regulatory T-cell (Treg) responses frequently detected in offspring to non-inherited maternal antigens. We test the hypothesis that the detection of very low copy levels of insertion/deletion (Indel) alleles consistent with non-inherited maternal genes, will correlate with immune regulation to non-inherited maternal antigens as detected by a trans-vivo Delayed-Type Hypersensitivity (tvDTH) assay in kidney transplant recipients, normal donors and their immediate biological family members. Preliminary data reported here compares qPCR amplification of rare DNA templates in the peripheral blood polymorphonuclear (PMN) fraction of cells, with the results of tvDTH assays for linked suppression of recall antigen responses in the presence of non-inherited maternal antigens [NIMA]. The two assays do not show a definitive correlation.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"5 1","pages":"80 - 85"},"PeriodicalIF":0.0,"publicationDate":"2014-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19381956.2015.1111974","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59984044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Exosomes: The missing link between microchimerism and acquired tolerance? 外泌体:微嵌合与获得性耐受性之间缺失的一环?

Chimerism Pub Date : 2014-10-02 DOI: 10.1080/19381956.2015.1082026

W. Burlingham

{"title":"Exosomes: The missing link between microchimerism and acquired tolerance?","authors":"W. Burlingham","doi":"10.1080/19381956.2015.1082026","DOIUrl":"https://doi.org/10.1080/19381956.2015.1082026","url":null,"abstract":"It has become increasingly clear that the immune system of viviparous mammals is much more in the business of acquiring tolerance to non-self antigens, than it is in rejecting cells that express them (for a recent review, highlighting the role of Treg cells, see ref.1). It is also clear that both self-tolerance, and acquired tolerance to non-self is a dynamic process, with a natural ebb and flow. As has been often said of an effective team defense in sports, tolerance will “bend but does not break.” How microchimerism, defined as the presence of extremely rare [1/104–1/106] cells of a genetically different individual, can induce either new immunogenetic pressures that push self-tolerance to the breaking point, or alternatively, provide relief from pre-existing immunogenetic risk, preventing development of autoimmune disease, remains a mystery. Indeed, the inability to directly correlate DNA-level microchimerism detected in blood samples by qPCR, with naturally occurring regulation to minor H and MHC alloantigens expressed by the rare cells themselves, has been frustrating to researchers in this field.2 [Haynes, W.J. et al, this issue] However, recent developments in the areas of transplantation and reproductive immunology offer clues to how the effects of microchimerism can be amplified, and how a disproportionate immune impact might occur from a very limited cell source.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"5 1","pages":"63 - 67"},"PeriodicalIF":0.0,"publicationDate":"2014-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19381956.2015.1082026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59984332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Complete donor chimerism is a prerequisite for the effect of Predicted Indirectly ReCognizable HLA Epitopes (PIRCHE) on acute graft-versus-host disease 完全的供体嵌合是预测间接可识别HLA表位(PIRCHE)在急性移植物抗宿主病中发挥作用的先决条件

Chimerism Pub Date : 2014-10-02 DOI: 10.1080/19381956.2015.1097025

K. Thus, R. D. de Weger, Talitha A de Hoop, Valeria E Boers Trilles, J. Kuball, E. Spierings

{"title":"Complete donor chimerism is a prerequisite for the effect of Predicted Indirectly ReCognizable HLA Epitopes (PIRCHE) on acute graft-versus-host disease","authors":"K. Thus, R. D. de Weger, Talitha A de Hoop, Valeria E Boers Trilles, J. Kuball, E. Spierings","doi":"10.1080/19381956.2015.1097025","DOIUrl":"https://doi.org/10.1080/19381956.2015.1097025","url":null,"abstract":"Predicted indirectly recognizable HLA epitopes (PIRCHE) computationally predict donor T-cell recognition of mismatched-HLA derived peptides following allogeneic haematopoietic stem-cell transplantation (allo-HSCT), as is evidenced by the correlation between presence of HLA-DPB1-derived PIRCHE and the occurrence of graft-vs.-host disease (GVHD). Complete donor T-cell chimerism associates with an increased GVHD risk compared to mixed patient and donor chimerism. If the correlation between the presence of PIRCHE and GVHD occurrence is indeed mediated by donor T cells, the presence of donor T cells should be required to observe such a correlation. This study was initiated to investigate whether the effect of PIRCHE is different in patients with complete chimerism compared to those with mixed chimerism. Indeed, the correlation between PIRCHE and GVHD is present in patients with complete chimerism, whereas it is absent in those with mixed chimerism. The data presented here suggest that chimerism status is important for the detection of potential GVHD epitopes.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"5 1","pages":"94 - 98"},"PeriodicalIF":0.0,"publicationDate":"2014-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19381956.2015.1097025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59984080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Mixed chimerism evolution is associated with T regulatory type 1 (Tr1) cells in a β-thalassemic patient after haploidentical haematopoietic stem cell transplantation 混合嵌合进化与单倍体造血干细胞移植后β-地中海贫血患者的T调节性1型(Tr1)细胞有关

Chimerism Pub Date : 2014-10-02 DOI: 10.1080/19381956.2015.1103423

M. Andreani, M. Gianolini, M. Testi, M. Battarra, Galluccio Tiziana, A. Morrone, P. Sodani, G. Lucarelli, M. Roncarolo, S. Gregori

{"title":"Mixed chimerism evolution is associated with T regulatory type 1 (Tr1) cells in a β-thalassemic patient after haploidentical haematopoietic stem cell transplantation","authors":"M. Andreani, M. Gianolini, M. Testi, M. Battarra, Galluccio Tiziana, A. Morrone, P. Sodani, G. Lucarelli, M. Roncarolo, S. Gregori","doi":"10.1080/19381956.2015.1103423","DOIUrl":"https://doi.org/10.1080/19381956.2015.1103423","url":null,"abstract":"In a cohort of β-Thalassemia (β-Thal) transplanted with haploidentical-HSCT we identified one transplanted patient characterized by persistent mixed chimerism (PMC) for several months after HSCT. In this unique β-Thal patient we assessed the donor engraftment overtime after transplantation, the potential loss of the non-shared HLA haplotype, and the presence of CD49b+LAG-3+ T regulatory type 1 (Tr1) cells, previously demonstrated to be associated with PMC after HLA-related HSCT for β-Thal. The majority of the patient's erythrocytes were of donor origin, whereas T cells were initially mostly derived from the recipient, no HLA loss, but an increased frequency of circulating Tr1 cells were observed. For the first time, we showed that when the proportion of residual donor cells decreases, the frequency of CD49b+LAG-3+ Tr1 cells declines, reaching the levels present in healthy subjects. These findings confirm previous results obtained in transplant related settings for β-Thal, and supported the central role of Tr1 cells in promoting and maintaining PMC after allo-HSCT.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"8 1","pages":"75 - 79"},"PeriodicalIF":0.0,"publicationDate":"2014-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19381956.2015.1103423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59984167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Pregnancy-induced maternal regulatory T cells, bona fide memory or maintenance by antigenic reminder from fetal cell microchimerism? 妊娠诱导的母体调节性T细胞，真实记忆还是通过胎儿细胞微嵌合的抗原提醒来维持?

Chimerism Pub Date : 2014-01-01 Epub Date: 2014-02-19 DOI: 10.4161/chim.28241

Jeremy M Kinder, Tony T Jiang, Dayna R Clark, Vandana Chaturvedi, Lijun Xin, James M Ertelt, Sing Sing Way

{"title":"Pregnancy-induced maternal regulatory T cells, bona fide memory or maintenance by antigenic reminder from fetal cell microchimerism?","authors":"Jeremy M Kinder, Tony T Jiang, Dayna R Clark, Vandana Chaturvedi, Lijun Xin, James M Ertelt, Sing Sing Way","doi":"10.4161/chim.28241","DOIUrl":"https://doi.org/10.4161/chim.28241","url":null,"abstract":"Long-term maintenance of immune components with defined specificity, without antigen is the hallmark feature of immunological memory. However, there are fundamental differences in how memory CD8(+) compared with CD4(+) T cells are maintained. After complete antigen elimination, CD8(+) T cells can persist as a self-renewing numerically stable cell population, and therefore satisfy the most stringent definition of \"memory.\" Comparatively, CD4(+) T cell maintenance is considerably less stable, often requiring low-level antigen persistence or antigenic reminders. Recent studies show these basic memory features, classically ascribed to effector CD8(+) and CD4(+) T cells, extend to immune suppressive Foxp3(+) regulatory CD4(+) T cells (Tregs). In particular, gestational expansion and postpartum retention of maternal Tregs with fetal specificity may explain the protective benefits of primary pregnancy on complications in subsequent pregnancy. Herein, the possibility of ongoing antigenic reminders from fetal cell microchimerism in postpartum maintenance of maternal Tregs with fetal specificity is considered.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"5 1","pages":"16-9"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.28241","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32141998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry. 应用流式细胞术检测小鼠胎儿免疫器官中母体造血微嵌合细胞。

Chimerism Pub Date : 2014-01-01 Epub Date: 2014-10-30 DOI: 10.4161/19381956.2014.959827

Maria Emilia Solano, Kristin Thiele, Ina Annelies Stelzer, Hans-Willi Mittrücker, Petra Clara Arck

{"title":"Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry.","authors":"Maria Emilia Solano, Kristin Thiele, Ina Annelies Stelzer, Hans-Willi Mittrücker, Petra Clara Arck","doi":"10.4161/19381956.2014.959827","DOIUrl":"https://doi.org/10.4161/19381956.2014.959827","url":null,"abstract":"Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45.1(+), respectively. Here, we now introduce the H-2 complex, which defines the major histocompatibility complex in mice and is homologous to HLA in human, as an additional target to detect maternal microchimerism among fetal haploidentical cells. We envision that this advanced approach to detect maternal microchimeric cells by flow cytometry facilitates the pursuit of phenotypic, gene expression and functional analysis of microchimeric cells in future studies. ","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"5 3-4","pages":"99-102"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/19381956.2014.959827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32887277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Maternal microchimerism in biliary atresia: are maternal cells effector cells, targets, or just bystanders? 胆道闭锁的母体微嵌合:母体细胞是效应细胞、靶细胞还是旁观者?

Chimerism Pub Date : 2014-01-01 Epub Date: 2014-03-26 DOI: 10.4161/chim.28576

Toshihiro Muraji

引用次数: 23

Does microchimerism mediate kin conflicts? 微嵌合是否调解亲属冲突?

Chimerism Pub Date : 2014-01-01 DOI: 10.4161/chim.29122

David Haig

引用次数: 10