Biodiversitas最新文献

{"title":"Isolation of Pediococcus pentosaceus to compete Vibrio harveyi in the shrimp Litopenaeus vannamei hatchery","authors":"RUBIYANTO WIDODO HALIMAN, METHODIUS DIGNA KURNIA, MOHAMMAD NURUL IMAN, SATRIA AJI KUSUMA, BENI HALALLUDIN, RIFKY RIZKIANTINO, PUTRI PURNAMA SARI, MUFTI RAHAYU, RACHMAWATI NUR FITRIANA, BESTRAN VIRLANDO PANJAITAN, ADINDA KINASIH JACINDA, HENDRI LAIMAN","doi":"10.13057/biodiv/d240853","DOIUrl":"https://doi.org/10.13057/biodiv/d240853","url":null,"abstract":"Abstract. Haliman RW, Kurnia MD, Iman MN, Kusuma SA, Halalludin B, Rizkiantino R, Sari PP, Rahayu M, Fitriana RN, Panjaitan BV, Jacinda AK, Laiman H. 2023. Isolation of Pediococcus pentosaceus to compete Vibrio harveyi in the shrimp Litopenaeus vannamei hatchery. Biodiversitas 24: 4514-4520. Shrimp is an aquaculture commodity with high economic value, and is widely cultured in Asia and South America. Among diseases in the shrimp hatchery, vibriosis is considered as the main problem, with emphasize on Vibrio harveyi infection. Probiotics are life microbes with defense mechanism against pathogens, and have been used in aquaculture as an alternative strategy in disease management through application into the water or through the feed. In order to renew the current probiotics available, exploration of probiotic candidate isolates with ability to compete with the luminous bacteria has been done in mangrove area of Merak Belantung, Kalianda, Lampung Province, Indonesia, and also from shrimp intestine, raised in the tank of research facility. Nine bacteria isolates were obtained from this exploration, and based on the references, 4 isolates had the potential to compete Vibrio harveyi. Based on the 16S rRNA sequencing result, those 4 isolates were Bacillus cereus, B. aryabhattai, Weisella paramesenteroides, and Pediococcus pentosaceus. Further analysis of in vitro test using agar-well diffusion inhibition method and in vivo test showed that among those 4 isolates, only P. pentosaceus had strong antibacterial activity against V. harveyi and could be used as probiotic in shrimp rearing with optimal dose at 5-10 µL L-1.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of bovine Eimeria species pathogenic using PCR ITS-1 in Indonesia","authors":"RADEN WISNU NURCAHYO, FITRINE EKAWASTI, LINTANG WINANTYA FIRDAUSY, VIKA ICHSANIA NINDITYA, MUKH FAJAR NASRULLOH, DYAH AYU KURNIAWATI, FATHUR ROHMAN HARYADI, JOKO PRASTOWO, DWI PRIYOWIDODO","doi":"10.13057/biodiv/d240850","DOIUrl":"https://doi.org/10.13057/biodiv/d240850","url":null,"abstract":"Abstract. Nurcahyo RW, Ekaswasti F, Firdausy LW, Ninditya VI, Nasrulloh MF, Kurniawati DA, Haryadi FR, Prastowo J, Priyowidodo D. 2023. Identification of bovine Eimeria species pathogenic using PCR ITS-1 in Indonesia. Biodiversitas 24: 4684-4689. Bovine eimeriosis is one of Indonesia's cattle's most significant health issues caused by the intestinal protozoan parasite Eimeria spp. Eimeria spp., E. zuernii, E. alabamensis, E. aburnensis, and E. bovis are recognized as most pathogenic since they cause clinical symptoms. This study aimed to identify pathogenic Eimeria spp. and explore their evolutionary relationships using the molecular marker, the internal transcribed spacer-1 (ITS-1). A total of 171 fecal samples were collected from the provinces of Banten, Lampung, and West Java, and 43 (25.1%) were infected with oocysts of Eimeria spp. by microscopic examination. The positive samples were further confirmed for different Eimeria species by amplifying and sequencing the ITS-1 gene. After editing and alignment, the sequences were considered for analyses, and the phylogenetic analysis confirmed four species of Eimeria such as E. bovis 39 (22.8%), E. zuernii 23 (13.5%), E. alabamensis 7 (4.09%), and E. aburnensis 25 (14.6%). In this study, several Eimeria species were related to overseas species; therefore, additional risk analysis is needed to control the spread of bovine eimeriosis. It is possible to learn important details regarding the ecology and population genetics of Eimeria spp. by employing sequencing and phylogenetic analysis to categorize all positive samples of the eimeriosis as pathogenic Eimeria spp.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population and spatial ecology of horseshoe crabs (Limulidae) in a nursery ground at Pulau Bedukang, Brunei Bay, Borneo","authors":"AHMAD FAHMI ROZAINI, KEVIN H. LAURIE, DAVID J. MARSHALL","doi":"10.13057/biodiv/d240829","DOIUrl":"https://doi.org/10.13057/biodiv/d240829","url":null,"abstract":"Abstract. Rozaini AF, Laurie KH, Marshall DJ. 2023. Population and spatial ecology of horseshoe crabs (Limulidae) in a nursery ground at Pulau Bedukang, Brunei Bay, Borneo. Biodiversitas 24: 4485-4492. Suitable nursery habitat to support populations of juvenile horseshoe crabs is becoming threatened globally. There are currently no formal studies on juvenile populations of the horseshoe crabs (Limulidae) for Brunei Darussalam (Brunei, Borneo). We investigated body size structure, population cohort and ecological distribution of juvenile horseshoe crabs associated with a mudflat on Pulau Bedukang, in Brunei Bay. Seventy juveniles were retrieved during four sampling days covering two seasons and four study sites. Carcinoscorpius rotundicauda and Tachypleus gigas were common, whereas only two individuals of Tachypleus tridentatus were found. Carapace width (size) was similar in the common species and there was no difference in juvenile size between the seasons and study sites. Body weight to carapace width relationships showed that the species grew at similar rates, although C. rotundicauda developed faster, producing 14 compared to the eight pre-migration mudflat instars produced by T. gigas. Species densities were similar, and there was no spatial separation of species or juvenile size across the mudflat or in relation to the mangrove forest edge. Nearest-neighbor (NN) analysis showed that individual crabs were generally dispersed. Their mudflat cohabitation suggests physiological and behavioral plesiomorphies for common optimal developmental salinity and temperature conditions. This study provides baseline information for comparison with other regional studies and for local conservation planning.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mangrove diversity and its relationships with environmental conditions in Kuala Bubon Village, West Aceh, Indonesia","authors":"WINTAH WINTAH, KISWANTO KISWANTO, ENDANG HILMI, MOH. HUSEIN SASTRANEGARA","doi":"10.13057/biodiv/d240864","DOIUrl":"https://doi.org/10.13057/biodiv/d240864","url":null,"abstract":"Abstract. Wintah, Kiswanto, Hilmi E, Sastranegara MH. 2023. Mangrove diversity and its relationships with environmental conditions in Kuala Bubon Village, West Aceh, Indonesia. Biodiversitas 24: 4599-4605. Mangrove ecosystem plays various ecological and socio-economic roles. Indonesia has the largest extent of mangrove forest one of which is located in West Aceh Regency, Aceh Province. The aim of this study was to assess the diversity and structure of mangrove forest in Kuala Bubon Village, West Aceh, and to analyze the relationships between the existence of mangrove species and the environmental conditions. Field survey was conducted from May to August 2022 using plot sampling method on three stations representing various habitat characteristics. Vegetation sampling using a 10 x 10 m2 plot as well as measurement on physical parameters (pH, salinity, and temperature) and chemical parameters (sediment texture and organic carbon content) were conducted. The relationship between the presence of mangrove species and the environmental parameters was analyzed using Principal Component Analysis (PCA). The result of vegetation sampling recorded 6 mangrove species, i.e., Rhizophora stylosa Griff, Sonneratia alba Sm, Avicennia marina (Blume), c, and Sonneratia caseolaris (L) Engl. This study revealed that the presence of Rhizophora stylosa Griff was related to the conditions of the clay substrate, pH and temperature values. Meanwhile, the existence of Rhizophora apiculata Blume, Rhizophora mucronata Lamk, Sonneratia alba Sm, Sonneratia caseolaris (L) Engl, and Avicennia marina (Blume) was related to the conditions of the silt substrate, diameter and salinity. Based on the results of relationship analysis, silt substrate and salinity have a correlation to maintain the mangrove ecosystem.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgal diversity assessment of Laguna Lake (Philippines) tributaries using Next-Generation Sequencing for water quality","authors":"JOY ANN SANTOS, JULIUS AARON MEJIA, M.A. JOWINA GALARION, PIERANGELI VITAL","doi":"10.13057/biodiv/d240845","DOIUrl":"https://doi.org/10.13057/biodiv/d240845","url":null,"abstract":"Abstract. Santos JA, Mejia JA, Galarion MAJ, Vital P. 2023. Microalgal diversity assessment of Laguna Lake (Philippines) tributaries using Next-Generation Sequencing for water quality. Biodiversitas 24: 4636-4644. The species composition and density of microalgae have been successfully used as bioindicators of water quality as they respond quickly to any change in the water chemistry brought about by pollution. Laguna Lake in the Philippines, one of the country's most important hotspots for aquaculture, continues to be threatened by eutrophication, yet its microalgal profile has been understudied. The presence of microalgae, especially those identified to become causative agents of harmful algal blooms in lakes, can cause corresponding health effects in humans and the environment. This study determined microalgal diversity in samples from tributaries of Laguna Lake, Philippines, through 18S rRNA metagenomic sequencing or Next-Generation Sequencing (NGS). Out of the five sampling sites, only two sites passed the NGS quality control pipeline. Chlorella M.Beijerinck, dominated the eukaryotic microbial population in the Alabang-Cupang River sample, which is 95% of the total population. While in the Biñan River sample, relevant species found were Cyclotella scaldensis Muylaert & Sabbe, Teleaulax acuta (Butcher) Hill, Cryptomonas curvata Ehrenb., and Peridiniopsis penardii (Lemmerm.) Bourr. This is the first study to use NGS to detect microalgae in Laguna Lake and warrants further elucidation of the microalgal population through increased sampling efforts in other recreational and livelihood hotspots. Furthermore, the study results open the importance of implementing new schemes for water quality assessments focused on molecular-based approaches.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134989978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The current state of the cenopopulations of Adonis tianschanica (Adolf) Lipsch. (Ranunculaceae) in Southeast Kazakhstan","authors":"KANAT KULYMBET, NASHTAY MUKHITDINOV, SERIK KUBENTAYEV, KURALAY TYNYBAYEVA, ASEM TASTANBEKOVA, MERUYERT KURMANBAYEVA, YUSUFJON GAFFOROV, RAUSHAN KAPARBAY, MOLDIR ZHUMAGUL","doi":"10.13057/biodiv/d240817","DOIUrl":"https://doi.org/10.13057/biodiv/d240817","url":null,"abstract":"Abstract. Kulymbet K, Mukhitdinov N, Kubentayev S, Tynybayeva K, Tastanbekova A, Kurmanbayeva M, Gafforov Y, Kaparbay R, Zhumagul M. 2023. The current state of the cenopopulations of Adonis tianschanica (Adolf) Lipsch. (Ranunculaceae) in Southeast Kazakhstan. Biodiversitas 24: 4359-4372. Adonis tianschanica (Adolf) Lipsch. is a rare, sub-endemic species growing in the Tien Shan mountains with declining numbers, making it listed in the Red Book of Kazakhstan. The aim of the study is to investigate the distribution and abundance of A. tianschanica cenopopopulations (CP) in its natural habitats in southeastern Kazakhstan as well as assess soil characteristics, biological and arealogical features, including the ontogenetic structure of cenopopopulations. Five cenopopulations (CP) of A. tianschanica were identified, namely two CPs in Ketmen Ridge (Kegen Pass), two CPs in Dzungarian Alatau (Tekeli Gorge) and one CP in Terskey Alatau (Saryzhaz). Vegetation data were collected using the traditional methods of field geobotanical studies based on the ecological-physiognomic approach.Plant communities having dominant species from a single ecobiomorph and ecologically related species groups.We complemented the population analysis with a soil properties map compiled from existing cartographic and available literature. The results of this study indicated the low number of A. tianschanica individuals in all CPs, which is likely due to the biological characteristics of the species (i.e. low seed production and seedling recruitment and the lack of vegetative regeneration) as well as environmental factors, and anthropogenic impact, mainly grazing with. We found that the total number of individuals of A. tianschanica in the five cenopopulations was 106 individuals. The average density of the species varied from 1.3 to 3.1 plants/m2 with CP2 being the highest and CP5 the lowest. The five cenopopulations were classified based on the absolute maxima of ontogenetic groups. There were two maxima in the age composition of individuals in CPs 3 and 4: the first (18.8-42.1%) was a group of vegetative plants (v), and the second (21.1-50.0%) was a group of young generative plants (g1). The natural state of the habitat of this cenopopulation can be explained as more favorable than others. In CP2, the absolute maximum corresponded to vegetative plants group (v) (37.0%). In CP5, young generative individuals (g1) and mature generative (g2) predominated (from 30.8% to 38.4%). In terms of age composition, the state of A. tianschanica cenopopulations is considered relatively satisfactory but demonstrated a low level of abundance. It has been established that the state of the species populations in the studied regions is affected by soil conditions, changes in climatic conditions and anthropogenic impact.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of carbon sequestration enhancement through intensive silvicultural techniques using Shorea leprosula plantation in Central Kalimantan, Indonesia","authors":"M. ISKANDAR, ISKANDAR ZULKARNAEN SIREGAR, HARUNI KRISNAWATI","doi":"10.13057/biodiv/d240852","DOIUrl":"https://doi.org/10.13057/biodiv/d240852","url":null,"abstract":"Abstract. Iskandar M, Siregar IZ, Krisnawati H. 2023. Potential of carbon sequestration enhancement through intensive silvicultural techniques using Shorea leprosula plantation in Central Kalimantan, Indonesia. Biodiversitas 24: 4699-4713. Various sectors are involved in achieving Indonesia’s Enhanced Nationally Determined Contribution (NDC), including Forestry and Other Land Uses (FOLU) sector. Sustainable Forest Management (SFM) implementation by forest concessionaires (PBPHs) enormously contributes to land-based mitigation activities, primarily at the local level. One of the SFM activities is forest enrichment with Intensive Silvicultural (SILIN) technique using Shorea leprosula Miq. to enhance carbon stock and sequestration. This study was conducted in PT Sarmiento Parakantja Timber (PT Sarpatim) in Central Kalimantan (Indonesia) and used the measurement data from 32 purposive sampling plots. Each plot was 40m × 40m in size, constructed under three different SILIN patterns (conventional strip, effective strip, and gap) as well as natural forest stands. This study found that SILIN gap pattern had higher carbon stock and sequestration potential, compared to the natural forest (reference) stands, and reached a net sink condition after 30 years. For the stands of SILIN conventional and effective strip patterns, the carbon stocks were lower than the reference stand, even when the vegetation aged 30 years. PBPHs applying SILIN technique as part of the SFM and the mitigation actions demonstrate an enhanced carbon stock and sequestration. This effort supports Indonesia’s Enhanced NDC target and FOLU Net Sink 2030.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial interaction between rice stunt virus and its insect vector Nilaparvata lugens Stal based on life table","authors":"LISTIHANI LISTIHANI, I GUSTI AYU DIAH YUNITI, PUTU EKA PASMIDI ARIATI, NI PUTU PANDAWANI, DEWA GEDE WIRYANGGA SELANGGA, I GEDE RAI MAYA TEMAJA, GUSTI NGURAH ALIT SUSANTA WIRYA, I PUTU SUDIARTA","doi":"10.13057/biodiv/d240851","DOIUrl":"https://doi.org/10.13057/biodiv/d240851","url":null,"abstract":"Abstract. Listihani L, Yuniti IGAD, Ariati PEP, Pandawani NP, Selangga DGW, Temaja IGRM, Wirya GNAS, Sudiarta IP. 2023. Beneficial interaction between rice stunt virus and its insect vector Nilaparvata lugens Stal based on life table. Biodiversitas 24: 4690-4698. The brown planthopper (Nilaparvata lugens) causes direct and indirect damage as a stunting virus vector in rice. Insect vectors and viruses are closely related with respect to disease transmission to host plants. Thus, this research aimed to determine the specific relationship between a virus and its insect vector on the biology and demography statistics of viruliferous brown planthopper (BPH). Research method included the propagation of N. lugens and stunt virus inoculum, the observation of cohort, stadia duration and egg survival rates, imago life span, and N. lugens life table. The BPH life table was arranged with the jackknife method for two treatments: plants infected by stunt virus and healthy plants. The research result showed that plants infected by stunt virus can shorten the BPH nymphal development stage from instar 2 to instar 5, life cycle, preoviposition period, and double time. Plants infected by stunt virus did not influence the BPH hatching pattern but influenced the total number of hatching eggs. The next BPH generation of stunt virus inflected plants increased 29.51 times more from the previous generation, while on healthy plants, the next BPH generation only increased 27.51 times. Virus-infected plants generally appeared to be superior quality hosts for vectors compared to uninfected plants, thus enhancing vector life table and virus spread.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The anatomical structure of the root, stem, and branch of Gyrinops versteegii trees from different growing sites","authors":"ARIEL ADIMAHAVIRA, GANIS LUKMANDARU, RINI PUJIARTI, FANANY WURI PRASTIWI, WIDYANTO DWI NUGROHO","doi":"10.13057/biodiv/d240863","DOIUrl":"https://doi.org/10.13057/biodiv/d240863","url":null,"abstract":"Abstract. Adimahavira A, Lukmandaru G, Pujiharti R, Prastiwi FW, Nugroho WD. 2023. The anatomical structure of the root, stem, and branch of Gyrinops versteegii trees from different growing sites. Biodiversitas 24: 4590-4598. Gyrinops versteegii (Gilg) Domke is an agarwood (Gaharu)-producing species with a unique color and very expensive fragrance. Even so, studies on the effects of growing sites on the wood anatomical structure of several parts of G. versteegii have not been thoroughly investigated. Therefore, this research aims to investigate the anatomical structure of G. versteegii on the tree's root, stem, and branch at two different growing sites. Three five-year-old G. versteegii trees from Pacitan and Klaten Districts (Indonesia) were used for the experiment and were harvested from the root, stem, and branch. The wood samples were then put in a glutaraldehyde solution for 24 hours and later in an alcohol solution. The samples were sliced in microtome (15-20 µm) and observed in the transverse, tangential, and radial sections. The results showed that the anatomical characteristics, such as vessel diameter, ray height, ray frequency, interxylary phloem proportion, and frequency differed significantly between growing sites. Furthermore, fiber length, vessel diameter, ray height, ray frequency, and interxylary phloem proportion significantly differed among plant parts. In addition, the branch part has the highest interxylary phloem value, indicating the possibility of a high agarwood resin deposit when agarwood stimulation is applied.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of morphological, micromorphological, anatomical structures and matK gene-based identification of aromatic litsea (Litsea cubeba)","authors":"ISMI NUR AINI, WISANTI WISANTI","doi":"10.13057/biodiv/d240859","DOIUrl":"https://doi.org/10.13057/biodiv/d240859","url":null,"abstract":"Abstract. Aini IN, Wisanti. 2023. Characterization of morphological, micromorphological, anatomical structures and matK gene-based identification of aromatic litsea (Litsea cubeba). Biodiversitas 24: 4557-4567. Aromatic litsea or krangean (Litsea cubeba (Lour.) Persoon) has potential as a drug. L. cubeba's status based on the IUCN red list is the least concern. This research aimed to (i) characterize morphology, micromorphology, and anatomy; (ii) indetify L. cubeba species based on DNA barcodes. The research samples were collected in the Mt. Anjasmoro, East Java, Indonesia at three different altitudes. Data in the form of various characters were analyzed using the one-way ANOVA method to determine the significance of the difference. The statistical test analysis of the quantitative character of L. cubeba showed a significant difference between heights (? = 0.05). The results showed that the characterization of L. cubeba includes (i) morphology: structure of lenticels, color, and substance that coats the lower surface of leaves, type of flower, fruit taste, and aroma; (ii) micromorphology: glands lining the abaxial surface of the lamina, trichomes on both surfaces of the lamina, structure of abaxial epidermal cells, the shape of pollen, shape and distribution of exin (iii) anatomy: cuticle thickness, epidermal cell shape, and presence of secretory cells in the lamina. The molecular identification in the form of L. cubeba barcode DNA based on the matK gene marker matched the Litsea cubeba sequence in NCBI (AB259073.1).","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135887521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}