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Retroviral-Based BMP-4 In Vivo Gene Transfer Strategy with Intramedullary Viral Delivery Optimizes Transgene Expression in Rat Femur Fractures 基于逆转录病毒的BMP-4体内基因转移策略与髓内病毒传递优化大鼠股骨骨折中的转基因表达

The open tissue engineering and regenerative medicine journal Pub Date : 2008-11-05 DOI: 10.2174/1875043500801010014

C. Rundle, Shin-Tai Chen, Ryan Porte, J. Wergedal, K. Lau

{"title":"Retroviral-Based BMP-4 In Vivo Gene Transfer Strategy with Intramedullary Viral Delivery Optimizes Transgene Expression in Rat Femur Fractures","authors":"C. Rundle, Shin-Tai Chen, Ryan Porte, J. Wergedal, K. Lau","doi":"10.2174/1875043500801010014","DOIUrl":"https://doi.org/10.2174/1875043500801010014","url":null,"abstract":"We have developed an intramedullary delivery strategy to administer retroviral vectors expressing a therapeutic gene to promote healing of a closed rat femur fracture. This strategy involves implantation of an indwelling catheter with the stabilizing Kirschner (K)-wire during the surgery prior to fracture of the femur by the three-point bending technique. It uses the openings in the bone that were already created for the stabilizing K-wire and the catheter insertion. In this study, transgene expression and callus bone formation induced by intramedullary delivery of MLV-based vectors expressing the bone morphogenetic protein-2/4 (BMP-2/4) hybrid gene or ￻-galactosidase (￻-gal) gene were compared with those pro- duced by percutaneous injections of the same vectors at the periosteum of the fracture site. The percutaneous injections of MLV-BMP-2/4 vector led to massive but asymmetric transgene expression in surrounding tissues within the fracture cal- lus and large amounts of supraperiosteal as well as asymmetric callus bone formation. In contrast, the intramedullary ad- ministration produced a robust and symmetric pattern of transgene expression at the fracture site with very minimal trans- duction at cells of surrounding tissues, resulting in normal subperiosteal bone development around the entire fracture cal- lus without supraperiosteal bone formation. In summary, we have developed an intramedullary retroviral vector delivery strategy with a rat femur fracture model that led to uniform transgene expression around the entire fracture site, which op- timizes the gene therapy-enhanced fracture repair. This strategy should readily be adapted to administer large dosages of any therapeutic vehicle (therapeutic molecules, peptides, or proteins, as well as viral or non-viral vectors) throughout much of early fracture repair, and thus it would be an ideal rat model for in vivo testing of various therapeutic agents to promote fracture repair.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"1 1","pages":"14-22"},"PeriodicalIF":0.0,"publicationDate":"2008-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sequential Use of Fibroblastic Growth Factor 2 and Transforming Growth Factor β1 in Cartilage Tissue Engineering Using Canine Chondrocytes 成纤维生长因子2和转化生长因子β1在犬软骨细胞软骨组织工程中的序贯应用

The open tissue engineering and regenerative medicine journal Pub Date : 2008-06-13 DOI: 10.2174/1875043500801010008

W. Gordon-Evans, M. Yaeger, M. Greenlee, M. Conzemius

引用次数: 0

Directed Cell Growth on Laser-Transferred 2D Biomaterial Matrices 激光转移二维生物材料基质上的定向细胞生长

The open tissue engineering and regenerative medicine journal Pub Date : 2008-03-28 DOI: 10.2174/1875043500801010001

A. Nógrádi, B. Hopp, T. Smausz, G. Kecskeméti, Z. Bor, L. Kolozsvári, A. Szabó, A. Klini, C. Fotakis

{"title":"Directed Cell Growth on Laser-Transferred 2D Biomaterial Matrices","authors":"A. Nógrádi, B. Hopp, T. Smausz, G. Kecskeméti, Z. Bor, L. Kolozsvári, A. Szabó, A. Klini, C. Fotakis","doi":"10.2174/1875043500801010001","DOIUrl":"https://doi.org/10.2174/1875043500801010001","url":null,"abstract":"Patterned arrangement of living cells to form tissues on a given surface is the prerequisite of successful bioen- gineering, tissue building and biosensor technology. The aim of the present study was to deposit various biomaterials onto a given substrate to establish a patterned cellular matrix using laser-based technologies. We used the Pulsed Laser Deposi- tion and the Absorbing Film-Assisted Laser-Induced Forward Transfer methods to deposit various biomaterials, such as fibronectin, collagen and endothelial cell growth supplement. Subpicosecond KrF excimer laser was used for irradiation of the target materials. When cultured neuroectodermal stem cells, astrocytes, endothelial and neuroblastoma cells were layered on the deposited biomaterial patterns a guided growth of these cells was induced along the patterned thin film. Some cell types showed various interactions when approached each other. The above methods are suitable to build an ar- chitecture of substrates which supports and guides the growth of cells and may enable the cells to induce directed and rapid repair of injured tissues.","PeriodicalId":88761,"journal":{"name":"The open tissue engineering and regenerative medicine journal","volume":"1 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2008-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68107438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

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