Biomedical Research and Therapy最新文献

{"title":"Teucrium montanum extract drives effector and memory differentiation of CD8+ T cells","authors":"Jing Li, Shuanggang Ma, Yaling Wang, Manqiu Wang, Mingyan Li, Changxing Gao, Lingzhi Zhang, Yong Li, Yunbao Liu, Zora Dajić Stevanović, Nikola Tanić, Nebojsa Arsenijevic, Shishan Yu, Qing Zhu","doi":"10.15419/bmrat.v10i11.844","DOIUrl":"https://doi.org/10.15419/bmrat.v10i11.844","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139202427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe Hemolysis Triggered by Favism in a Female Patient with G6PD Deficiency: A Case Report","authors":"Nabilah Rameli, Justin Hor Hung Juan, Ling Pei Chi, Nurul Asyikin Nizam Akbar, Adibah Daud, Syamihah Mardhiah A. Razak, Sumaiyah Adzahar","doi":"10.15419/bmrat.v10i10.836","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.836","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139306397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing RNA extraction and library preparation from oral squamous cell carcinoma FFPE samples for next-generation RNA sequencing","authors":"Huong Thu Duong, Phuong Minh Pham, Nam Huynh Bao Tran, Phuong Thanh Huynh, Hung Trong Hoang, Thinh Huy Quoc Dang, Tu Anh Thai, Chi Thi Kim Nguyen, Nam Cong Nhat Huynh","doi":"10.15419/bmrat.v10i10.840","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.840","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139307598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ERP evaluation of EEG signals for monitoring anesthesia depth during surgery","authors":"Lamia Bouafif","doi":"10.15419/bmrat.v10i10.841","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.841","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and Management of Thrombotic Thrombocytopenic Purpura During Pregnancy: A Case Report","authors":"Sumaiyah Adzahar, Adibah Daud, Nabilah Rameli, Nik Fatma Fairuz Nik Mohd Hassan, Nur Aini Syakirah Ahmad Shuyuti","doi":"10.15419/bmrat.v10i10.837","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.837","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible Association of Three Polymorphisms in Cytokine TNF-a (238G/A, 308G/A, 1031T/C) with Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis","authors":"Pemula Gowtham, Karthick Harini, Anbazhagan Thirumalai, P. Pallavi, K. Girigoswami, A. Girigoswami","doi":"10.15419/bmrat.v10i10.839","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.839","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139309467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinatorial effects of metformin and glucose on the immune evasion of breast cancer 4T1 cells","authors":"Duy Khuong Pham, Hoai Nam Le, Anh Nhu Nguyen, Thao N. Huynh, Chau Nhat Truong, Van Phuc Pham","doi":"10.15419/bmrat.v10i10.835","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.835","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139309854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of circulating miRNA-218, miRNA-222, and miRNA-146 as biomarkers of polycystic ovary syndrome in epileptic patients receiving valproic acid","authors":"Mahya Rajabi, Seyed Mehdi Kalantar, Elham Mojodi, Mansour Salehi, Razieh Dehghani Firouzabadi, Seyed Masoud Etemadifar, Fateme Montazeri","doi":"10.15419/bmrat.v10i9.833","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.833","url":null,"abstract":"Introduction: This study was designed to evaluate the relationship between taking sodium valproate (VPA) and the onset of polycystic ovarian syndrome (PCOS) in women with epilepsy (WWE) and analyze the biochemical factors and expression levels of three miRNAs as diagnostic or predictive biomarkers. These miRNAs target numerous genes and molecular pathways involved in hyperandrogenism, insulin resistance, and obesity in PCOS patients. Methods: The study was conducted on 120 WWE aged 18–35 years before and after monotherapy with VPA and 120 women with PCOS (WWP). After collecting the plasma samples of patients, the total RNA was extracted. Then, the miRNA-218, miRNA-222, and miRNA-146 expression levels were determined via qRT– PCR. Results: In this study, the relative expression levels of miRNA-146 and miRNA-218 showed a significant increase in the WWP and treated epileptic patients (P < 0.01). However, the expression level of miRNA-222 significantly (P0.05) reduced. Our finding showed a significant increase in the concentrations of anti-Müllerian hormone (AMH), free testosterone, and estradiol and an increased LH/FSH ratio after treatment compared with pre-treatment with VPA (P < 0.05). Conclusion: Significant changes were observed in the expression of the examined miRNAs after receiving VPA, especially miRNA-218. In addition, a significant correlation was found between PCOS and AMH, free testosterone, estradiol, and the LH/FSH ratio. Therefore, the miRNA-218 expression and these biochemical factors are valuable biomarkers for predicting PCOS symptoms. They are cost-effective for controlling side effect and timely medication adjustments in patients receiving VPA.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epstein–Barr virus-encoded RNA expression and its relationship with the clinicopathological parameters of Vietnamese patients with nasopharyngeal carcinoma","authors":"Tran Ngoc Dung, Pham Van Thinh, Nguyen Mai Hanh, Nguyen Thuy Linh, Pham Van Thi, Dang Thai Tra, Truong Dinh Tien, Le Tai The, Nguyen Manh Hung, Dang Thanh Chung","doi":"10.15419/bmrat.v10i9.830","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.830","url":null,"abstract":"Introduction: Nasopharyngeal carcinoma (NPC) is a common malignancy in Viet Nam, and its pathogenesis is closely associated with Epstein–Barr virus (EBV) infection. However, the relationship between EBV infection and clinicopathological characteristics related to NPC prognosis in Vietnamese patients remains poorly understood. This study aimed to investigate the association between EBV infection and various clinical parameters in Vietnamese patients with NPC. Methods: We collected clinical data from 31 patients with histologically confirmed NPC and evaluated their samples for EBV-encoded RNA (EBER) expression using the chromosomal in situ hybridization (CISH) technique. We examined the relationship between EBER expression and several clinical parameters, including age, sex, lymph node metastasis, tissue invasion and metastasis, clinical stage, and histological NPC type. Results: The patients' ages ranged from 19 to 74 years, with 80.64% aged >40 years and 87.1% male. In addition, 80.65% had lymph node metastasis, and 38.71% had tissue invasion and distant metastasis. Most patients (67.74%) were diagnosed at a late stage (III or IV), with the most common histological type being type III (48.39%), followed by type I (29.03%) and II (22.58%). EBER expression was observed in 48.39% of the patients and was significantly associated with younger age (<40, p = 0.0362) and undifferentiated carcinoma (type III, p = 0.0007). However, EBER expression was not significantly associated with sex, lymph node metastasis, tissue invasion and metastasis, or clinical stage. Conclusions: Our study suggests that EBV infection may contribute to NPC pathogenesis. It also shows significant associations between EBV infection and younger age and undifferentiated carcinoma type. The CISH technique could help screen asymptomatic high-risk individuals, managing and predicting the NPC prognosis. The small sample size and single-center design limit the generalizability of our findings.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Alzheimer’s disease diagnosis using an XG-Boost model applied to MRI images","authors":"Khoi Nguyen, My Nguyen, Khiet Dang, Bao Pham, Vy Huynh, Toi Vo, Lua Ngo, Huong Ha","doi":"10.15419/bmrat.v10i9.832","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.832","url":null,"abstract":"Introduction: Early Alzheimer's disease (AD) diagnosis is critical to improving the success of new treatments in clinical trials, especially at the early mild cognitive impairment (EMCI) stage. This study aimed to tackle this problem by developing an accurate classification model for early AD detection at the EMCI stage based on magnetic resonance imaging (MRI). Methods: This study developed the proposed classification model through a machine-learning pipeline with three main steps. First, features were extracted from MRI images using FreeSurfer. Second, the extracted features were filtered using principal component analysis (PCA), backward elimination (BE), and extreme gradient (XG)-Boost importance (XGBI), the efficiency of which was evaluated. Finally, the selected features were combined with cognitive scores (Mini Mental State Examination [MMSE] and Clinical Dementia Rating [CDR]) to create an XG-Boost three-class classifier: AD vs. EMCI vs. cognitively normal (CN). Results: The MMSE and CDR had the highest importance weights, followed by the thickness of the left superior temporal sulcus and banks of the superior temporal lobe. Without feature selection, the model had the lowest accuracy of 69.0%. After feature selection and the addition of cognitive scores, the accuracy of the PCA, BE, and XGBI approaches improved to 74.0%, 90.9%, and 91.5%, respectively. The BE with tuning parameters model was chosen as the final model since it had the highest accuracy of 92.0%. The area under the receiver operating characteristic curve for the CN, AD, and EMCI classes were 0.98, 0.94, and 0.88, respectively. Conclusion: Our proposed model shows promise in early AD diagnosis and can be fine-tuned in the future through testing on a multi-dataset.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}