Biomedical Research and Therapy最新文献

{"title":"The cytotoxic effect of Vernonia amygdalina Del. extract on myeloid leukemia cells","authors":"Nguyen Trung Quan, Bui Thi Kim Ly, Hoang Thanh Chi","doi":"10.15419/bmrat.v10i8.827","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.827","url":null,"abstract":"Introduction: This study aimed to demonstrate the cytotoxic effect of a bitter leaf (Vernonia amygdalina Del.) ethanol extract on myeloid leukemia cells. Methods: The plant extract was prepared using the maceration method. The toxicity assays used the trypan blue exclusion method. Flow cytometry and reverse transcription PCR methods were used to deduce the mechanism of action. Results: The V. amygdalina Del. extract strongly affected K562 cells, with a half-maximal inhibitory concentration of 8.78 ± 2.224 µg/mL. The extract could induce apoptosis and arrest the cell cycle in K562 cells. The extract increased the mRNA levels of caspase 3 (CASP3), baculoviral IAP repeat containing 5 (BIRC5/survivin), and phosphatidylinositol 3-kinase (PI3K) and decreased the mRNA levels of retinoblastoma transcriptional corepressor 1 (RB1/pRB), B cell lymphoma/leukemic 2 (BCL2), BCL2-like 1 (BCL2L1/BCL-XL), caspase 9 (CASP9), and the breakpoint cluster region (BCR)-Abelson (ABL) fusion gene. Conclusion: The V. amygdalina Del. extract strongly inhibited the acute myeloid leukemia cell line K562. It was found to arrest the cell cycle and induce apoptosis by regulating the expression of related genes that predicted targeting BCR-ABL downregulation.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute Myeloid Leukemia with 8:21 Translocation and Aberrant B-Marker Expression","authors":"Nur Ilyia Syazwani Saidin, Razan Hayati Zulkeflee, Abdul Hanan Abdullah, Mohd Nazri Hassan, Marne Abdullah, Nurul Asyikin Nizam Akbar, Noor Haslina Mohd Noor","doi":"10.15419/bmrat.v10i8.822","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.822","url":null,"abstract":"Background: Acute myeloid leukemia (AML) with t(8;21)(q22;q22) is a frequently encountered subtype of AML with recurrent genetic abnormalities, found in approximately 1–5% of AML cases. Here, we present cases of AML with t(8;21) in elderly patients with aberrant B-marker expression identified at our institution, including their clinical outcomes when treated with hypomethylating agents and BCL-2 inhibitors. Case presentation: A 60-year-old patient diagnosed with AML carried the t(8;21) chromosomal translocation. Immunophenotyping and immunohistochemistry revealed aberrant expression of B-markers, including CD19, CD79a, and PAX5. Cytogenetic analysis also identified a loss of the X chromosome, a common cytogenetic aberration in AML associated with t(8;21). Due to the patient's age and inability to tolerate intensive chemotherapy, treatment was initiated using a hypomethylating agent and a BCL-2 inhibitor. Although the initial bone marrow evaluation showed an excess of blast cells, subsequent assessments demonstrated a favorable response to the treatment, with the absence of blast cells and improvements in peripheral blood parameters. Conclusion: The presence of B-marker expression in AML with t(8;21) is a relatively common occurrence. The integration of cytogenetic and molecular investigations plays a vital role in accurately diagnosing and classifying AML. A remarkable feature of AML with t(8;21) is its high remission rate, and this holds true even in cases where standard intensive chemotherapy is not utilized. Moreover, the detection of aberrant B-marker expression, particularly CD19, signifies a favorable prognosis.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case series report: simultaneous internal fixation of multiple fractures","authors":"Toan Thanh Vo, Thai Hoa Thi Nguyen, Kha Dong To, Luc Bao Nguyen, Duc Thien Nguyen, Dat Thanh Ha, Vien Hoang Ngo, Ngan Doan, Quang Van Le","doi":"10.15419/bmrat.v10i8.828","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.828","url":null,"abstract":"Background: Polytrauma is often associated with a high mortality rate and requires intensive management. Although several cases of polytrauma have been reported as being related to thoracic or brain injury, there are few reports concerning multiple fractures. We aimed to present a case series report about polytrauma with multiple fractures, highlighting several clinical importance and management strategies. Case presentation: The first case is a 31-year-old male patient who was admitted to the emergency department with multiple injuries from violent abuse, including intraperitoneal bladder rupture, right pulmonary contusion, bilateral closed femoral shaft fractures, bilateral closed humeral shaft fractures, and bilateral closed radius and ulna shaft fractures. Case 2 is a 41-year-old female who was involved in a motorcycle accident in a head collision, with multiple wounds and multiple fractures, including the right distal third radius, ulna, 2nd metacarpal, right distal third tibia, and fibula. The last case is a 25-year-old comatose male patient who was hospitalized with several wounds and bruises on the right lower extremity and left forearm after a traffic accident. He was diagnosed with a concussion, crush wound of the right foot with metatarsal fractures, and a closed fracture of the right middle third femur, right middle third tibia, and left distal third radius. Three cases of multiple trauma were reported, in which we successfully internally fixed four to eight fractures at once without any complications after the procedure. The patient's wound status at admission, such as whether they have an open fracture or a complex wound that could become infected and result in sepsis or hemorrhagic shock, should be a crucial factor considered when deciding whether to perform emergency orthopedic surgery. Additionally, patients who have been properly stabilized and do not have any concomitant conditions or concurrent soft tissue injuries can receive early total care. Patient awareness is a critical sign for serial CT brain scans and additional surveillance before definitive fracture fixation. Conclusion: This report can serve as a reference for management decisions in future instances within the context of national healthcare capacity.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between Angiotensin Converting Enzyme Insertion−Deletion Polymorphisms and Cardiovascular Risk Factor Determinants in Urban and Rural Populations","authors":"Ismail Ismail, Muhammad Basri, Mardiana Mustafa, Ade Nukhotimah","doi":"10.15419/bmrat.v10i8.825","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.825","url":null,"abstract":"Background: The angiotensin converting enzyme insertion-deletion (ACE I/D) polymorphism located on chromosome 17q23 (287 bp in intron 16) is associated with cardiovascular risk factors (CRFs), but results vary among populations, which is thought to be the cause of ethnic differences. This study explored the role of the ACE I/D polymorphism and its correlation with CRF determinants among urban and rural groups. Methods: A total of 182 male and female participants were recruited in the age range of 20 – 55 years for CRF determinant examination and ACE gene polymorphism (n = 140). Results: Most samples examined for polymorphic ACE genes showed increased CRF determinants in the two groups. For genotype II and urban group ID, the risk was increased 5 – 8 times for the CRF of obesity. The frequency of genotype II significantly increased the incidence of CRFs of smoking and sedentary by 1 – 3 times in both groups. Conclusions: The ACE I/D polymorphism has a differential effect on both urban and rural groups. Smoking, sedentary behavior, and obesity were risk factors for CRF in both groups. Therefore, an overall design strategy for health policies is needed to mitigate the burden of cardiovascular disease, which ends in death in both groups.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Engineering Biology Problems Book: Bridging the gap between biomedicine and engineering","authors":"Ilya. D. Klabukov, Denis S. Baranovskii","doi":"10.15419/bmrat.v10i8.821","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.821","url":null,"abstract":"Progress in the biological sciences requires advanced approaches to biological education. The current well-established paradigm rarely uses engineering design to solve biological problems. Engineering biology is a novel science field and academic discipline that focuses on the engineering of living objects using biological techniques. We believe that the integration of engineering components into biological education together with a wide application of engineering methods can provide considerable benefits to the education system. We developed the ``Engineering Biology Problems Book'' to bridge the gap between biology, medicine, and engineering.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136034895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Interleukin 6 Immunohistochemical and Plasma Levels in Invasive Ductal Carcinoma Breast: A Cross-Sectional Study","authors":"Ankita Girdhar, Kalyani Raju, Krishna Prasad","doi":"10.15419/bmrat.v10i8.826","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.826","url":null,"abstract":"Background: Breast carcinoma (BC) is one of the most common malignancies in women, affecting 1 in 8.Interleukin 6 (IL6) is a proinflammatory cytokine. The role of IL6 pathways in breast cancer motivated the development of anti-IL6 agents or monoclonal antibodies, which inhibit the IL6/signal transducer and activator of transcription 3 (STAT3) pathway. This study aimed to determine the proportion and intensity of immunohistochemical (IHC) IL6 expression in invasive ductal carcinoma (IDC) breast tissue sections and estimate plasma IL6 levels using an enzyme-linked immunosorbent assay (ELISA) in the same patients to evaluate the association between IHC and plasma IL6 levels. Methods: This laboratory observational cross-sectional study examined new primary BC cases between January 2021 and June 2022. IL6 IHC was performed on tissue sections and analyzed using the histochemical (H)-score system. Plasma samples were taken from the same cases to estimate IL6 levels using an ELISA. The data were analyzed for an association between IHC and plasma IL6 levels in paired samples using SPSS software (version 22). Results: Among 50 IDC cases, the mean IHC-based IL6 H-score was 201.6 ± 88.4, and the mean ELISA-based plasma IL6 level was 68.13 ± 89.98 pg/mL. A slight positive correlation existed between IHC-based IL6 H-scores and ELISA-based plasma IL6 levels (p = 0.217). IHC-based IL6 H-scores were not associated with clinicopathological parameters. ELISA-based plasma IL6 levels were significantly associated with premenopausal status (p = 0.010), positive erb-b2 receptor tyrosine kinase 2 (ERBB2/HER2/NEU) expression (p = 0.040), low marker of proliferation Ki-67 (MKI67) index (p = 0.040), and the ERBB2/HER2/NEU enriched molecular category (p = 0.040). Conclusion: IHC-based IL6 H-scores increased with ELISA-based plasma IL6 levels among the cases. ELISA-based plasma IL6 levels were significantly associated with premenopausal status, positive ERBB2/HER2/NEU expression, low Ki-67 proliferative index, and the ERBB2/HER2/NEU enriched molecular category. Therefore, plasma IL6 could be a potential marker to assess prognosis in patients with IDC.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatocellular adenoma in a child: a case report","authors":"Truong Dinh Khai, T. Phuong, Phung Nguyen Viet Hung, Le Minh Huy, Nguyen Ngoc Minh Khanh","doi":"10.15419/bmrat.v10i7.816","DOIUrl":"https://doi.org/10.15419/bmrat.v10i7.816","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43479010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cavitary lesion from pulmonary nocardiosis in non-immunocompromised patients with chronic lung disease: A report of three cases","authors":"Khoa Nguyen-Dang, Vu Le-Thuong, Lam Nguyen-Ho, Nguyen Tran-Ngoc","doi":"10.15419/bmrat.v10i7.817","DOIUrl":"https://doi.org/10.15419/bmrat.v10i7.817","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47692732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genes involved in premature aging and their association with age-related diseases: A mini review","authors":"Gopikrishna Agraharam, A. Girigoswami, Pemula Gowtham, K. Girigoswami","doi":"10.15419/bmrat.v10i7.819","DOIUrl":"https://doi.org/10.15419/bmrat.v10i7.819","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49441209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural genomics and immunoinformatics analyses of non-structural protein 6 (NSP6) and its probable role in autophagy","authors":"N. Yashvardhini, D. Jha, P. Khan, Amit Kumar, K. Pranay","doi":"10.15419/bmrat.v10i7.818","DOIUrl":"https://doi.org/10.15419/bmrat.v10i7.818","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46420684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}