Biomedical Research and Therapy最新文献

{"title":"Possible Association of Three Polymorphisms in Cytokine TNF-a (238G/A, 308G/A, 1031T/C) with Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis","authors":"Pemula Gowtham, Karthick Harini, Anbazhagan Thirumalai, P. Pallavi, K. Girigoswami, A. Girigoswami","doi":"10.15419/bmrat.v10i10.839","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.839","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"14 6 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139309467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinatorial effects of metformin and glucose on the immune evasion of breast cancer 4T1 cells","authors":"Duy Khuong Pham, Hoai Nam Le, Anh Nhu Nguyen, Thao N. Huynh, Chau Nhat Truong, Van Phuc Pham","doi":"10.15419/bmrat.v10i10.835","DOIUrl":"https://doi.org/10.15419/bmrat.v10i10.835","url":null,"abstract":"","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"21 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139309854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of circulating miRNA-218, miRNA-222, and miRNA-146 as biomarkers of polycystic ovary syndrome in epileptic patients receiving valproic acid","authors":"Mahya Rajabi, Seyed Mehdi Kalantar, Elham Mojodi, Mansour Salehi, Razieh Dehghani Firouzabadi, Seyed Masoud Etemadifar, Fateme Montazeri","doi":"10.15419/bmrat.v10i9.833","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.833","url":null,"abstract":"Introduction: This study was designed to evaluate the relationship between taking sodium valproate (VPA) and the onset of polycystic ovarian syndrome (PCOS) in women with epilepsy (WWE) and analyze the biochemical factors and expression levels of three miRNAs as diagnostic or predictive biomarkers. These miRNAs target numerous genes and molecular pathways involved in hyperandrogenism, insulin resistance, and obesity in PCOS patients. Methods: The study was conducted on 120 WWE aged 18–35 years before and after monotherapy with VPA and 120 women with PCOS (WWP). After collecting the plasma samples of patients, the total RNA was extracted. Then, the miRNA-218, miRNA-222, and miRNA-146 expression levels were determined via qRT– PCR. Results: In this study, the relative expression levels of miRNA-146 and miRNA-218 showed a significant increase in the WWP and treated epileptic patients (P < 0.01). However, the expression level of miRNA-222 significantly (P0.05) reduced. Our finding showed a significant increase in the concentrations of anti-Müllerian hormone (AMH), free testosterone, and estradiol and an increased LH/FSH ratio after treatment compared with pre-treatment with VPA (P < 0.05). Conclusion: Significant changes were observed in the expression of the examined miRNAs after receiving VPA, especially miRNA-218. In addition, a significant correlation was found between PCOS and AMH, free testosterone, estradiol, and the LH/FSH ratio. Therefore, the miRNA-218 expression and these biochemical factors are valuable biomarkers for predicting PCOS symptoms. They are cost-effective for controlling side effect and timely medication adjustments in patients receiving VPA.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epstein–Barr virus-encoded RNA expression and its relationship with the clinicopathological parameters of Vietnamese patients with nasopharyngeal carcinoma","authors":"Tran Ngoc Dung, Pham Van Thinh, Nguyen Mai Hanh, Nguyen Thuy Linh, Pham Van Thi, Dang Thai Tra, Truong Dinh Tien, Le Tai The, Nguyen Manh Hung, Dang Thanh Chung","doi":"10.15419/bmrat.v10i9.830","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.830","url":null,"abstract":"Introduction: Nasopharyngeal carcinoma (NPC) is a common malignancy in Viet Nam, and its pathogenesis is closely associated with Epstein–Barr virus (EBV) infection. However, the relationship between EBV infection and clinicopathological characteristics related to NPC prognosis in Vietnamese patients remains poorly understood. This study aimed to investigate the association between EBV infection and various clinical parameters in Vietnamese patients with NPC. Methods: We collected clinical data from 31 patients with histologically confirmed NPC and evaluated their samples for EBV-encoded RNA (EBER) expression using the chromosomal in situ hybridization (CISH) technique. We examined the relationship between EBER expression and several clinical parameters, including age, sex, lymph node metastasis, tissue invasion and metastasis, clinical stage, and histological NPC type. Results: The patients' ages ranged from 19 to 74 years, with 80.64% aged >40 years and 87.1% male. In addition, 80.65% had lymph node metastasis, and 38.71% had tissue invasion and distant metastasis. Most patients (67.74%) were diagnosed at a late stage (III or IV), with the most common histological type being type III (48.39%), followed by type I (29.03%) and II (22.58%). EBER expression was observed in 48.39% of the patients and was significantly associated with younger age (<40, p = 0.0362) and undifferentiated carcinoma (type III, p = 0.0007). However, EBER expression was not significantly associated with sex, lymph node metastasis, tissue invasion and metastasis, or clinical stage. Conclusions: Our study suggests that EBV infection may contribute to NPC pathogenesis. It also shows significant associations between EBV infection and younger age and undifferentiated carcinoma type. The CISH technique could help screen asymptomatic high-risk individuals, managing and predicting the NPC prognosis. The small sample size and single-center design limit the generalizability of our findings.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Alzheimer’s disease diagnosis using an XG-Boost model applied to MRI images","authors":"Khoi Nguyen, My Nguyen, Khiet Dang, Bao Pham, Vy Huynh, Toi Vo, Lua Ngo, Huong Ha","doi":"10.15419/bmrat.v10i9.832","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.832","url":null,"abstract":"Introduction: Early Alzheimer's disease (AD) diagnosis is critical to improving the success of new treatments in clinical trials, especially at the early mild cognitive impairment (EMCI) stage. This study aimed to tackle this problem by developing an accurate classification model for early AD detection at the EMCI stage based on magnetic resonance imaging (MRI). Methods: This study developed the proposed classification model through a machine-learning pipeline with three main steps. First, features were extracted from MRI images using FreeSurfer. Second, the extracted features were filtered using principal component analysis (PCA), backward elimination (BE), and extreme gradient (XG)-Boost importance (XGBI), the efficiency of which was evaluated. Finally, the selected features were combined with cognitive scores (Mini Mental State Examination [MMSE] and Clinical Dementia Rating [CDR]) to create an XG-Boost three-class classifier: AD vs. EMCI vs. cognitively normal (CN). Results: The MMSE and CDR had the highest importance weights, followed by the thickness of the left superior temporal sulcus and banks of the superior temporal lobe. Without feature selection, the model had the lowest accuracy of 69.0%. After feature selection and the addition of cognitive scores, the accuracy of the PCA, BE, and XGBI approaches improved to 74.0%, 90.9%, and 91.5%, respectively. The BE with tuning parameters model was chosen as the final model since it had the highest accuracy of 92.0%. The area under the receiver operating characteristic curve for the CN, AD, and EMCI classes were 0.98, 0.94, and 0.88, respectively. Conclusion: Our proposed model shows promise in early AD diagnosis and can be fine-tuned in the future through testing on a multi-dataset.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of trocar site, intraperitoneal, and laparoscopically guided posterior transversus abdominis muscle bupivacaine injection for reducing pain after laparoscopic hysterectomy surgery: A double-blind randomized clinical trial","authors":"Masoomeh Nataj Majd, Zahra Asgari, Narjes Marjani, Parand Gheshlaghi, Mahroo Rezaeinejad, Mina Fatehnejad","doi":"10.15419/bmrat.v10i9.831","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.831","url":null,"abstract":"Background: Few studies have investigated the pain-reducing effects of bupivacaine after laparoscopic hysterectomy. Therefore, this study compared the efficacy of three methods of bupivacaine injection—subcutaneous injection into the trocar site, intraperitoneal injection, and posterior transversus abdominis muscle block under laparoscopic guidance—for reducing pain after laparoscopic hysterectomy; the efficacy of each method was also compared with that of placebo. Methods: This double-blind randomized clinical trial study included 95 patients with good general health who underwent elective laparoscopic hysterectomy for benign disease in 2021. The patients were allocated into three intervention groups (subcutaneous injection of 10 cc bupivacaine 0.25%, heavy under trocar sites; 10 cc bupivacaine 0.25%, heavy injection into the transversus abdominis plane block; and 10 cc bupivacaine 0.25%, heavy intraperitoneal injection) and a control group. Abdominal and shoulder pain 2–4, 8, 12, and 24 h after the surgery were compared between groups. Results: The four groups were homogenous in age, weight, height, body mass index, surgery duration, surgery type, and family history of cancer (P > 0.05). The mean abdominal and shoulder pain score significantly decreased from the first time point (hours 2–4) to 8, 12, and 24 hours after surgery in the trocar site, intraperitoneal, and control groups (P < 0.001). However, we did not observe a significant decrease in abdominal and shoulder pain in the transversus abdominis plane block group (P > 0.05). Conclusion: The present study indicates that bupivacaine administration methods of transversus abdominis plane block and trocar site injection are effective and safe for reducing pain following laparoscopic hysterectomy.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tridax procumbens leaf antioxidants and hormonal activity ameliorate variable stress-induced erectile and reproductive impairments in Wistar rats","authors":"Salami Shakiru Ademola, Amasiatu Chioma Irenemarie, Allen Michael Olabode, Salahdeen Hussein Mofomosara, Murtala Babatunde Adekunle","doi":"10.15419/bmrat.v10i9.834","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.834","url":null,"abstract":"Introduction: The role of Tridax procumbens leaf extract in erectile dysfunction (ED) of chronic variable stress (CVS) etiology is unknown. This study investigates the potential of the ethanol fraction of Tridax procumbens leaf (EETP) in modulation of CVS-induced ED. Methods: Twenty-five male Wistar rats were divided into five groups of five rats each. Groups 1 &amp; 2 (without stress) were treated with normal saline (vehicle) and 100 mg/kg of EETP, respectively. Groups 3–5 were treated as stress groups, with Group 3 co-treated with 100 mg/kg of EETP, and group 4 co-treated with vitamin C (7 mg/kg). Treatments were administered by oral gavage once daily for seven weeks. Oxidative biomarkers, cortisol, testosterone, and sperm parameters were determined, as well as the contractile mechanism of the corpus cavernosa to cumulative doses of calcium chloride, potassium chloride, acetylcholine, and sodium nitroprusside. Furthermore, the contractile mechanism was also determined after incubation in acetovanillone, nicorandil, methyl blue, and glibenclamide. Results: Serum cortisol was significantly reduced, while testosterone was significantly increased in the EETP supplemented groups when compared to the CVS-only exposed group. Furthermore, malonaldehyde activity was decreased while superoxide dismutase concentration was increased in the EETP- and vitamin C-supplemented groups when compared to the CVS-only exposed group. Contraction (%) responses to calcium chloride and potassium chloride were also significantly reduced in the CVS-only exposed group when compared to the EETP-supplemented groups. The relaxation responses (%) to acetylcholine and SNP were significantly increased in the CVS group supplemented with EETP and vitamin C when compared to the CVS-only exposed group. The incubation of the cavernosa tissues in acetovanillone and nicorandil resulted in increased relaxation (%) in the CVS-only group, while incubation in glibenclamide caused increased relaxation in the EETP-supplemented groups compared to CVSonly exposed group. Sperm motility (%) was significantly reduced while abnormal spermatozoa was increased in the CVS-only exposed group when compared to the groups supplemented with EETP and Vitamin C. Conclusion: Variable stress-induced dysfunctions in erectile mechanism were attenuated through supplementation with EETP.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocrine-disrupting pesticides and SARS-CoV-2 infection: Role of ACE2, TMPRSS2 and CD147","authors":"Swati Dixit, Haseeb Ahsan, Fahim Halim Khan","doi":"10.15419/bmrat.v10i9.829","DOIUrl":"https://doi.org/10.15419/bmrat.v10i9.829","url":null,"abstract":"COVID-19 is a global pandemic caused by severe acute respiratory syndrome (SARS) coronavirus- 2 (SARS-CoV-2). The three main receptors used by SARS-CoV-2 to bind and gain entry into human cells are ACE, TMPRSS2, and CD147. These molecular factors have crucial roles in human metabolism and homeostasis, but the upregulation of these factors causes severe diseases such as myocarditis, prostate cancer, and other endocrine-related cancers. Studies have found that once humans come into contact with SARS-CoV-2, the chances of being affected by such disorders increase; indeed, infection with the virus is associated with increased morbidity and mortality from heart attacks and pulmonary inflammation. Notably, exposure to some pesticides, such as chlorpyrifos, cypermethrin, and imidacloprid, which are identified as potential endocrine disruptors, causes such disorders by interfering with hormonal signaling pathways, such as the insulinglucagon pathway and the thyroid pathway. This review focuses on the potential role of pesticides in exacerbating the comorbidities linked with SARS-CoV-2 and their effect on the molecular factors associated with SARS-CoV-2. Understanding the potential therapeutic implications of this link between SARS-CoV-2 severity and pesticides requires further clinical trials and investigations.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135126628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmablastic transformation of multiple myeloma post-autologous hematopoietic stem cell transplant","authors":"Nurul Asyikin Nizam Akbar, Mohd Nazri Hassan, Salfarina Iberahim, Nur Ilyia Syazwani Saidin, Wardah Roslan, Nur Ain Izzati Abd Halim, Abu Dzarr Abdullah, Hany Hakimi Wan Hanafi, Nur Diyana Mohd Shukri, Sumaiyah Adzahar","doi":"10.15419/bmrat.v10i8.820","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.820","url":null,"abstract":"The present case report describes the uncommon and adverse plasmablastic transformation of multiple myeloma (MM) following autologous hematopoietic stem cell transplantation. To the best of our knowledge, this is the first case of plasmablastic myeloma (PBM) after an autologous hematopoietic transplant to be reported in Malaysia. A 41-year-old man initially diagnosed with MM IgG kappa reported lower back pain symptoms for a year, along with other associated symptoms. After receiving several lines of chemotherapy, the patient displayed a partial response (PR), and an autologous stem cell transplant (ASCT) was subsequently performed. Two months after the transplant, the patient showed signs of anemia, with a hemoglobin level of 8.0 g/dL. A peripheral blood film revealed the presence of a leucoerythroblastic blood film with normocytic normochromic red blood cells and rouleaux formation but no apparent plasma cells. The main infiltrating cells in the bone marrow aspirate (BMA) and trephine biopsy were plasmablasts with kappa light chain restriction. An increase in serum kappa free light chain (FLC), serum lambda FLC, and a low albumin/globulin (A/G) ratio were observed. In addition, serum protein electrophoresis showed an IgG kappa paraprotein band in the gamma region. Post-ASCT, the disease transformed into PBM, which conferred a poor prognosis on thepatient despite his post-transplant status. This case report highlights the diagnostic challenges of plasmablastic transformation in MM. Diagnosing PBM is thus crucial for the prompt and proper management of affected patients. Another consideration in the present case is whether the transplant procedure itself or the immunopathogenesis that took place after the ASCT resulted in the subsequent transformation into PBM.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"187 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of cancer stem cell enrichment between spheroids derived from single-cell and multicellular aggregate cultures","authors":"Omar Nafiis Hairuddin, Badrul Hisham Yahaya, Mohamad Johari Ibahim, Abhi Verakumarasivam, Chan Soon Choy, Musalmah Mazlan, Nurhidayah Ab. Rahim, Syarifah Masyitah Habib Dzulkarnain, Siti Farizan Mansor","doi":"10.15419/bmrat.v10i8.823","DOIUrl":"https://doi.org/10.15419/bmrat.v10i8.823","url":null,"abstract":"Introduction: Cancer stem cells (CSCs) represent a distinct group of cells within cancerous tissue that possess the ability to initiate tumorigenesis and exhibit potency, self-renewal, and drug resistance. The study of CSCs often encounters challenges in obtaining these cells of interest or generating a sufficient quantity for downstream analysis. Nevertheless, it is feasible to enrich CSCs in vitro by subjecting them to conditions that stimulate their CSC properties, such as prolonged exposure to drugs or radiation, or by promoting their self-renewal capability through spheroid culture. Spheroids are a specific type of cell culture that organizes cells into a three-dimensional structure, closely mimicking the in vivo environment. These spheroids consist of a heterogeneous cell population, including CSCs or tumor-propagating cells responsible for tumor growth and maintenance. In our study, we cultured spheroids derived from single cells as well as multicellular aggregates to enrich CSCs based on their self-renewal capability and the structural organization provided by the three-dimensional context. Methods: Comparing the spheroid cultures with the parental adherent monolayer cells, we observed higher expression of CSC markers, pluripotent genes, and adipogenic differentiation in both multicellular spheroids (MCS) and single cell-derived spheroids (SCDS) of the two tested cell lines. Results: The spheroids exhibited progressive growth in size throughout the culture period. When comparing the two methods, SCDS demonstrated greater expression of surface markers and all three pluripotent genes associated with CSCs. Furthermore, when assessing drug resistance potential and the expression of the ABCG2 drug efflux gene, only 5637 SCDS displayed increased resistance to cisplatin and upregulation of ABCG2. Conclusion: In conclusion, both the MCS and SCDS methods effectively enriched the population of bladder CSCs in the 5637 and HT-1376 bladder cancer cell lines. However, the SCDS method demonstrated a higher upregulation of CSC markers and pluripotent gene expression compared to MCS. It is worth noting that spheroid culture and CSC enrichment are not mutually exclusive and can coexist with increased chemotherapy resistance and upregulation of ABCG2 drug efflux gene expression. Moreover, the drug efflux capability may vary depending on the specific cell line and clonal lineage. These strategies can serve as valuable models for CSC enrichment, the study of cancer cell behavior, disease modeling, and personalized chemotherapy investigations.","PeriodicalId":8870,"journal":{"name":"Biomedical Research and Therapy","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136034898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}