Biologija最新文献_第8页

Evaluation of different methods for preventing the African swine fever virus 预防非洲猪瘟病毒不同方法的评价

Biologija Pub Date : 2020-03-29 DOI: 10.6001/biologija.v66i1.4191

S. Pilevičienė, V. Jurgelevičius, Žygimantas Janeliūnas, G. Pridotkas, P. Mačiulskis, R. Krikštolaitis, A. Paulauskas

{"title":"Evaluation of different methods for preventing the African swine fever virus","authors":"S. Pilevičienė, V. Jurgelevičius, Žygimantas Janeliūnas, G. Pridotkas, P. Mačiulskis, R. Krikštolaitis, A. Paulauskas","doi":"10.6001/biologija.v66i1.4191","DOIUrl":"https://doi.org/10.6001/biologija.v66i1.4191","url":null,"abstract":"2 Vytautas Magnus University, K. Donelaičio g. 58, Kaunas 44248, Lithuania African swine fever (ASF) is a contagious disease of key importance that occurs in wild boar population and domestic pigs. To date, there is no vaccine or treatment available, hence disease surveillance and stamping out in case of an outbreak is the only way to control it. A duplex ASF-IC real-time PCR assay was optimised using field samples obtained in Lithuania and results of singleplex and duplex real-time PCR assays allowing the identification of the ASF virus were compared. The specificity and sensitivity of ASF virus detection was validated using a reference sample of positive and negative virus nucleic acid and ASF virus positive and negative samples obtained from animals in Lithuania. Results prove that duplex ASF-IC real-time PCR assay provides a rapid, sensitive, and reliable molecular tool for ASF virus detection in wild boar population and domestic pigs. In general, it was found that duplex ASF-IC real-time PCR assay proves to be faster and more sensitive than singleplex real-time PCR and therefore has a high potential to be applied in routine analysis.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75203232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The first record of the Siberian northern shrike (Lanius borealis sibiricus) in Lithuania with molecular confirmation 立陶宛西伯利亚北伯劳鸟(Lanius borealis sibiricus)的首次记录与分子鉴定

Biologija Pub Date : 2020-03-29 DOI: 10.6001/biologija.v66i1.4187

Vytautas Eigirdas, Vesta Jonikė

引用次数: 0

Influence of gibberellin inhibitors on the accumulation and redistribution of various forms of carbohydrates and nitrogen-containing compounds in plants of Solanum melongena L. 赤霉素抑制剂对茄内各种碳水化合物和含氮化合物积累和再分配的影响。

Biologija Pub Date : 2020-03-29 DOI: 10.6001/biologija.v66i1.4189

V. Rogach, K. Reshetnyk, V. Kuryata, T. Rogach

{"title":"Influence of gibberellin inhibitors on the accumulation and redistribution of various forms of carbohydrates and nitrogen-containing compounds in plants of Solanum melongena L.","authors":"V. Rogach, K. Reshetnyk, V. Kuryata, T. Rogach","doi":"10.6001/biologija.v66i1.4189","DOIUrl":"https://doi.org/10.6001/biologija.v66i1.4189","url":null,"abstract":"2 Department of Botany and Ecology, Faculty of Chemistry, Biology and Biotechnologies, Vasyl’ Stus Donetsk National University, 600th-anniversary Street, 21, Vinnytsia, 21021, Ukraine The 0.025% tebuconazole (EW-250), 0.25% chlormequat chloride (CCC-750), and 0.15% ethephon (2-CEPA) influences growth processes, the leaf apparatus, chlorophyll concentration and various carbohydrates forms, and total nitrogen content in Solanum melongena L. The decrease in the linear dimensions of the aubergine was established for all preparations. The most significant decrease in height was observed after the application of 2-HEPCs. The retardants increased the number of leaves, the dry matter mass of leaves, the leaf area, chlorophyll content, and the surface density of the leaf. EW-250 and ССС-750 retardants strengthened carbohydrate accumulation in aubergine fruits, mainly due to reducing forms. During the growing season, the total and protein nitrogen content decreased in roots and stems both in control and in experiment variants. EW-250 and CCC-750 accelerated total and protein nitrogen outflow from roots and stems and increased its content in leaves. Increasing the carbohydrates content in fruits the retardants simultaneously reduced the content of various nitrogen forms. Such changes in the dynamics of total nitrogen under the influence of tebuconazole led to enhanced fruit formation on each plant. Thus, retardants EW-250 and ССС-750 changed the nature of the donor-acceptor relations in them by increasing the outflow of plastic substances from vegetative organs to fruits and increasing the culture productivity.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73821589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Amorphous state stability of plant vitrification solutions 植物玻璃化溶液的非晶态稳定性

Biologija Pub Date : 2020-03-29 DOI: 10.6001/biologija.v66i1.4190

K. Vozovyk, O. Bobrova, A. Prystalov, N. Shevchenko, L. Kuleshova

{"title":"Amorphous state stability of plant vitrification solutions","authors":"K. Vozovyk, O. Bobrova, A. Prystalov, N. Shevchenko, L. Kuleshova","doi":"10.6001/biologija.v66i1.4190","DOIUrl":"https://doi.org/10.6001/biologija.v66i1.4190","url":null,"abstract":"3 Department of Low Temperature Preservation, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Pereyaslavskaya St. 23, Kharkiv 61015, Ukraine Two critical factors for obtaining high viable biological samples after cryopreservation by vitrification method are cryoprotectant glass-forming ability while cooling and the stability of its amorphous state during warming. The present work is devoted to the study of the amorphous state stability of plant vitrification solutions (PVS) by differential scanning calorimetry method. The objects of the study were PVS1 modified, PVS2, PVS3 modified, PVS4 and PVSN. The thermograms of PVSs, frozen in liquid nitrogen, were recorded at a warming rate of 0.5 deg/min. The glass transition temperatures of PVS1, PVS2 and PVS3 were –109.0°C, –115.3°C, and –93.9°C, respectively. Neither exonor endothermic peaks have been recorded, indicating no crystallization, at the cooling and the warming stages. PVS4 and PVSN thermograms, besides glass transition (–111.5°C and –110.0°C, correspondingly), showed crystallization from the amorphous state (devitrification) and ice melting peaks. Crystallization enthalpy of PVSN was 2.8 times higher compared to PVS4. This fact testifies that the crystalline phase was larger in PVSN compared to PVS4. It should be noted that crystallization in PVS4 and PVSN may be related to the low warming rate used during the experiment. At higher warming rates, this crystallization is likely to be avoided. Thus, PVS1, PVS2 and PVS3 have a high glass-forming ability and a stabile amorphous state. The amorphous state stability of PVS4 and PVSN is significantly lower compared to PVS1, PVS2, and PVS3.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85902226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Pathogen screening in the red fox (Vulpes Vulpes) from Lithuania 立陶宛赤狐病原菌的筛选

Biologija Pub Date : 2020-01-04 DOI: 10.6001/biologija.v65i4.4122

Povilas Sakalauskas, I. Lipatova, Jana Radzijevskaja, A. Paulauskas

引用次数: 4

Diseases of different aetiologies in salmonids in Ukraine 乌克兰鲑鱼中不同病因的疾病

Biologija Pub Date : 2020-01-04 DOI: 10.6001/biologija.v65i4.4121

N. Matvienko, M. Maistrenko, L. Buchatsky, A. Didenko

{"title":"Diseases of different aetiologies in salmonids in Ukraine","authors":"N. Matvienko, M. Maistrenko, L. Buchatsky, A. Didenko","doi":"10.6001/biologija.v65i4.4121","DOIUrl":"https://doi.org/10.6001/biologija.v65i4.4121","url":null,"abstract":"Institute of Fisheries, National Academy of Agricultural Sciences, 135 Obukhivska St., Kyiv 03164, Ukraine The paper contains the results of the studies into the ichthyopathological situation of the invasion and infectious diseases of salmonids carried out by the Ichthyopathology Laboratory of the Institute of Fisheries of Ukraine from 2013 to 2016. The investigated salmonid species included: the rainbow trout Oncorhynchus mykiss, the brown trout Salmo trutta morpha fario, the brook trout Salvelinus fontinalis, and the European grayling Thymallus thymallus. The fish were sampled from aquatculture farms as well as natural water bodies and their diseases included invasive (parasitic) and infectious (viral and bacterial). The most frequently encountered invasions in the brown and brook trout were ciliates: Chilodonella pisci cola and Apiosoma conicum, Trichodina (in particular T. truttae and T. nigra), Ichtyophthirius multifiliis; monogenea Gy rodactylus birmani, and diplostoma Diplostomum spathaceum. Aeromonas and Flavobacterium bacteria were isolated from fry and young-of-the-year rainbow trout and brook trout. Y. ruckeri positive samples were isolated from the fish with the signs of yersiniosis. As for viral diseases, IPNV isolates (the first time in Ukraine) of the rainbow trout were first isolated in fish farms in the western regions of Ukraine. A phylogenetic analysis of these IPNV isolates was performed, which showed that they belonged to Sp strain and the European genotype.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75746634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of gibberellin and retardants on the germination of seeds with different types of reserve substances under the conditions of skoto- and photomorphogenesis 赤霉素和缓凝剂对不同类型储备物质种子在光形态和光形态发生条件下萌发的影响

Biologija Pub Date : 2020-01-04 DOI: 10.6001/biologija.v65i4.4123

I. Poprotska, V. Kuryata, О. Khodanitska, S. Polyvanyi, L. Golunova, Yuriy Prysedsky

{"title":"Effect of gibberellin and retardants on the germination of seeds with different types of reserve substances under the conditions of skoto- and photomorphogenesis","authors":"I. Poprotska, V. Kuryata, О. Khodanitska, S. Polyvanyi, L. Golunova, Yuriy Prysedsky","doi":"10.6001/biologija.v65i4.4123","DOIUrl":"https://doi.org/10.6001/biologija.v65i4.4123","url":null,"abstract":"2 Vasyl Stus Donetsk National University, 600-richya St., 21, Vinnytsia 21021, Ukraine The influence of preparations of phytohormone gibberellin and antigibberellin–retardants tebuconazole and chlormequat chloride – on the germination of plant seeds with different types of reserve compounds in the light (photomorphogenesis) and in the dark (skotomorphogenesis) was studied. It was established that under the action of gibberellin, stimulation of the aboveground part and root system growth of maize, beans and pumpkin seedlings was more intensive in comparison with the control. This process was faster in the dark. The use of tebuconazole and chlormequat chloride significantly inhibited the process of germination both in the light and in the dark. At the same time, the coefficient of the use of reserve substances was maximal under the action of gibberellin, and minimum under the action of retardants, both under the conditions of skotoand photomorphogenesis. The change in the growth characteristics and the coefficient of the use of reserve substances of bean seeds was accompanied by a decrease in the content of total nitrogen, indicating the use of reserve nitrogen-containing compounds in the processes of morphogenesis. The content of protein nitrogen in the control was lower under the conditions of skotomorphogenesis than in the photomorphic seedlings, and the opposite effect was noted for the actions of gibberellin and the retardant. In the later stages of germination, the largest reserve oil of pumpkin seed remained in cotyledonary leaves of photomorphic plants under the effects of chlormequat chloride, which clearly correlated with the least intense growth rates of seedlings in this variant, both in the light and darkness. Under conditions of skotomorphogenesis the growth stimulating effect of gibberellin significantly increased, and the light blocked the action of this phytohormone.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"33 1-2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78191941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

DNA barcoding in some Belarusian insects 一些白俄罗斯昆虫的DNA条形码

Biologija Pub Date : 2020-01-04 DOI: 10.6001/biologija.v65i4.4120

S. Dromashko, Alexey A. Semianiak, N. Balashenko

{"title":"DNA barcoding in some Belarusian insects","authors":"S. Dromashko, Alexey A. Semianiak, N. Balashenko","doi":"10.6001/biologija.v65i4.4120","DOIUrl":"https://doi.org/10.6001/biologija.v65i4.4120","url":null,"abstract":"3 State Scientific and Production Amalgamation “Scientific and Practical Center for Bioresources of the National Academy of Sciences of Belarus”, 27 Akademicheskaya St., 22072, Minsk, Republic of Belarus Due to a number of factors in the anthropogenic load on ecosystems, environmental changes, and competition between native and invasive species, the loss of species diversity has currently risen to an alarming scale. One of the prospective approaches to remedy the current state and halt the loss is the international project “Barcode of Life”. The report describes the results of molecular genetic research on the insect representatives of such orders as Coleoptera (beetles), Lepidoptera (butterflies), and Trichoptera (caddisflies) using the cytochrome c oxidase subunit 1 of the mitochondrial region (COI). We proposed a technique for the sampling of biological material to isolate DNA from the hind legs of individuals. The technique prevents removal of the individuals of the most valuable species collected on the territory of the Republic of Belarus from their habitats. The DNA sample collection of order representatives was investigated. For the subfamily Cetoniinae (flower chafer), significant differences in haplotypes among the representatives of the Belarusian and European parts of their areal were found. For Trichoptera, it was shown that the COI gene has high variability to differentiate species. It was also revealed that some species of caddisflies, which Belarusian researchers believe to be synonymous ones, have sequences with big differences according to the BoldSystem database. This fact should be explored in the future. So, our analysis allows considering the COI gene as a satisfactory marker for the species identification in the Belarusian insects’ taxa studied.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84400048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-accidental structural chromosome aberrations in established monkey B-lymphocyte cell lines 已建立的猴b淋巴细胞细胞系的非偶然结构染色体畸变

Biologija Pub Date : 2020-01-04 DOI: 10.6001/biologija.v65i4.4119

D. E. Araviashvili, O. P. Chzhu, I. Marinich, I. Danilova

{"title":"Non-accidental structural chromosome aberrations in established monkey B-lymphocyte cell lines","authors":"D. E. Araviashvili, O. P. Chzhu, I. Marinich, I. Danilova","doi":"10.6001/biologija.v65i4.4119","DOIUrl":"https://doi.org/10.6001/biologija.v65i4.4119","url":null,"abstract":"FSBSI Research Institute of Medical Primatology, 177 Mira St., Veseloe, Adler region, Sochi 354376, Krasnodar krai, Russia Established primate lymphocyte cell lines obtained from tumour samples and from EBV-positive monkeys served us as the model system for studying the role of genetic factors and chromosomal abnormalities in malignization. The investigation of chromosome regions and genes involved in chromosomal aberrations leading to malignization in these lines was the aim of our work. Cytogenetic analysis was performed at different stages of cultivation in vitro. To determine the oncogenes and tumour suppressor genes located on aberrant chromosomes, data on mapping rhesus macaque genes, and high similarity of human and monkey karyotypes were used. We found that, in the line obtained from lymphomatous baboon tissue, the inactivation of tumour suppressor gene RB1 on chromosome 17 after chromosomal rearrangement is one of the most probable causes of in vivo malignization. Chromosomal aberrations at the region of oncogene c-Ki-ras and tumour suppressor gene TP53 change the proliferative status and differentiation in established cell lines obtained from healthy but EBV-seropositive primates. The other cause of malignization in these lines is an increase in expression of the oncogene c-myc caused by trisomy of chromosome 8 where c-myc is located. Structural aberrations in established primate cell lines affecting several chromosomal loci were identified as: (1) causing the proto-oncogene activation – the central event in the tumour clone occurrence, and (2) deactivating tumour suppressor genes. The change in the chromosome number leads to increase in oncogenic products and to damage of regulatory functions associated with cell proliferation.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88924551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of laser irradiation on the development of vegetative mycelium Pleurotus ostreatus 激光照射对平菇营养菌丝体发育的影响

Biologija Pub Date : 2020-01-04 DOI: 10.6001/biologija.v65i4.4118

K. Reshetnyk, Yuriy Prysedsky, D. Yuskov

{"title":"The influence of laser irradiation on the development of vegetative mycelium Pleurotus ostreatus","authors":"K. Reshetnyk, Yuriy Prysedsky, D. Yuskov","doi":"10.6001/biologija.v65i4.4118","DOIUrl":"https://doi.org/10.6001/biologija.v65i4.4118","url":null,"abstract":"Department of Plant Physiology and Biochemistry, Vasyl’ Stus Donetsk National University, 600-richya St. 21, Vinnytsia 21021, Ukraine The article presents the results of the study on the influence of laser irradiation on the development of vegetative mycelium and the period of the occurrence of the corcules of the fruit bodies of Pleurotus ostreatus. It has been established the growth processes of P. ostreatus, can be best stimulated by applying laser irradiation of mycelium with a green spectrum of light for 10 s. According to this mode of exposure, the best increase in the growth rate of mycelium of 38.3% and the appearance of the largest number of rudiments of the fruiting bodies were recorded. Laser irradiation of mycelium for 10 s with red and blue light spectrum increased the growth rate of mycelium from 7.41 to 20.4%, respectively, and the number of rudiments of the fruiting bodies increased by 1.5 to 2 times. Laser irradiation of the mycelium with 5 s, 15 s, and 20 s with red, blue, and green light spectra did not have a significant effect on the growth processes of P. ostreatus. These data open significant prospects for the modification of the existing cultivation technologies, which would increase the economic efficiency of the biotechnological cultivation process of P. ostreatus.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82800343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3