Biologija最新文献_第7页

Molecular characterization of Anaplasma phagocytophilum infection in the cervids and feeding ticks from Lithuania 立陶宛血吸虫和吸血蜱嗜吞噬细胞无原体感染的分子特征

Biologija Pub Date : 2020-09-30 DOI: 10.6001/BIOLOGIJA.V66I3.4308

Jana Radzijevskaja, Justina Snegiriovaitė, Artūras Kibiša., I. Ražanskė, A. Paulauskas

{"title":"Molecular characterization of Anaplasma phagocytophilum infection in the cervids and feeding ticks from Lithuania","authors":"Jana Radzijevskaja, Justina Snegiriovaitė, Artūras Kibiša., I. Ražanskė, A. Paulauskas","doi":"10.6001/BIOLOGIJA.V66I3.4308","DOIUrl":"https://doi.org/10.6001/BIOLOGIJA.V66I3.4308","url":null,"abstract":"1 Vytautas Magnus University, K. Donelaičio g. 58, 44248 Kaunas, Lithuania Anaplasma phagocytophilum is a bacterial pathogen, which is a major cause of zoonotic disease, anaplasmosis. The main vectors of A. phagocytophilum are ticks of the Ixodes ricinus complex. A. phagocytophilum has a broad geographic distribution and a high degree of biological and clinical diversity. Epidemiological studies in multiple countries have shown that the prevalence of A. phagocytophilum highly depends on the density of ticks and their potential hosts such as the cervids, which are one of the main sources of nutrition for Ixodes ticks. In Lithuania, the cervids are important game animals but their contribution as reservoirs for A. phagocytophilum remains unknown. The objectives of the study were to investigate the prevalence of A. phagocytophilum infections in the cervids and feeding ticks and to characterize the A. phagocytophilum strains obtained from the cervids and ticks based on sequence analysis of msp4 gene. A total of 187 ticks were collected from 44 cervids (roe deer, red deer, and moose) harvested by professional hunters during the hunting seasons of 2010–2013 and 2016–2017 in Lithuania. Blood and spleen samples were collected from 29 animals (27 roe deer and two red deer). A. phagocytophilum DNA was identified in ten (37.04%) of the 27 roe deer. The overall prevalence of A. phagocytophilum in I. ricinus and D. reticulatus ticks was 39.3% (70/178) and 22.2% (2/9) respectively. The sequence analysis of the msp4 gene of A. phagocytophilum revealed nine different sequence types: five msp4 sequence types were detected in ticks and seven in roe deer.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79974114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of antimicrobial activity of synthesized 9H-alkylcarbazole and 10H-alkylphenothiazine derivatives on the cells of Salmonella enterica ser. Typhimurium, Saccharomyces cerevisiae, and Candida albicans 合成的9h -烷基咔唑和10h -烷基吩噻嗪衍生物对肠道沙门氏菌细胞的抑菌活性评价。鼠伤寒菌，酿酒酵母菌和白色念珠菌

Biologija Pub Date : 2020-06-13 DOI: 10.6001/biologija.v66i2.4255

S. Sutkuvienė, S. Sakalauskaite, Neringa Kuliešienė, L. Ragelienė, R. Daugelavičius

{"title":"Evaluation of antimicrobial activity of synthesized 9H-alkylcarbazole and 10H-alkylphenothiazine derivatives on the cells of Salmonella enterica ser. Typhimurium, Saccharomyces cerevisiae, and Candida albicans","authors":"S. Sutkuvienė, S. Sakalauskaite, Neringa Kuliešienė, L. Ragelienė, R. Daugelavičius","doi":"10.6001/biologija.v66i2.4255","DOIUrl":"https://doi.org/10.6001/biologija.v66i2.4255","url":null,"abstract":"2 Department of Biochemistry, Faculty of Medicine, Lithuanian University of Health Sciences, Tilžės str. 18, 47181 Kaunas, Lithuania 10H-substituted phenothiazine and 9H-substituted carbazole derivatives are important because of a very wide range of applications and especially in medical chemistry due to their pharmacological activities. In this study, we synthesized 9H-alkylcarbazole and 10H-alkylphenothiazine derivatives with various lengths of alkyl chains and evaluated their antimicrobial and efflux inhibiting activities on the cells of Salmonella enterica ser. Typhimurium, Saccharomyces cerevisiae, and Candida albicans. Results of our study revealed that an increased length of alkyl chains of the carbazoles increased the accumulation of efflux indicator tetraphenylphosphonium (TPP+) ions. Cells of S. enterica efflux mutant ΔTolC had a considerable susceptibility to the synthesized compounds. The compounds exerted synergy with fluconazole against S. cerevisiae yeast. Efflux pump mutant ΔPdr5 was hypersensitive to the investigated carbazole and phenothiazine derivatives. The inhibitory effect of the compounds with a shorter alkyl chain (10-methyl-10H-phenothiazine and 9-methyl-9H-carbazole) was the highest for Candida albicans cells.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82560101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional analysis of the network of protected areas of the Vinnytsia region as the basis of a regional ecological network 作为区域生态网络基础的文尼察地区保护区网络的结构和功能分析

Biologija Pub Date : 2020-06-13 DOI: 10.6001/biologija.v66i2.4253

T. Kucher, Yu. I. Ovchinnikova

{"title":"Structural and functional analysis of the network of protected areas of the Vinnytsia region as the basis of a regional ecological network","authors":"T. Kucher, Yu. I. Ovchinnikova","doi":"10.6001/biologija.v66i2.4253","DOIUrl":"https://doi.org/10.6001/biologija.v66i2.4253","url":null,"abstract":"2 Vasyl’ Stus Donetsk National University, 600-richya St. 21, Vinnytsia 21021, Ukraine Planning an ecological network requires special attention to the functionality of the created elements. Thus, in the case of the Vinnytsia Regional Ecological Network (REN), protection and restoration of typical landscape complexes are needed. The structure of the ecological network is branched, but heterogeneous; it is potentially capable of expansion and optimization. As the development of the REN of the Vinnytsia region is based on objects and territories of the Nature Reserve Fund (NRF), the structural and functional optimization of the eco-network requires optimization of the structure of the NRF. The purpose of the research is to study the spatial structure of the ecological network for the functional completeness and the effectiveness of ensuring the conservation of biodiversity. This paper analyses the structure and functions of the Vinnytsia REN, demonstrates dynamics of the formation of the NRF network and the distribution of protected objects among administrative regions, and proposes ways for its optimization. The results showed the necessity of a revision of the current structure and creation of new zoological and landscape reserves in order to improve the functional efficiency of the network. The results suggest paying more attention to providing multifunctionality to eco-network elements, as well as avoiding the creation of island objects.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77720173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of laser irradiation on the growth and biosynthetic indicators of basidiomycete strains of carotenoid synthesis 激光照射对类胡萝卜素担子菌生长及生物合成指标的影响

Biologija Pub Date : 2020-06-13 DOI: 10.6001/biologija.v66i2.4258

A. Shelest, A. Velygodska, D. Kramarenko

引用次数: 0

Impact of forest management on wild boar (Sus scrofa) population in south Lithuania 立陶宛南部森林管理对野猪(Sus scrofa)种群的影响

Biologija Pub Date : 2020-06-13 DOI: 10.6001/biologija.v66i2.4254

Tomas Barkauskas, L. Griciuvienė, O. Belova, Nomeda Bratčikovienė

引用次数: 0

Prevalence and co-infection of mosquito- and tick-borne pathogens in domestic dogs suspected for canine babesiosis in Lithuania 立陶宛疑似犬巴贝斯虫病的家养狗中蚊媒和蜱媒病原体的流行和共感染情况

Biologija Pub Date : 2020-06-13 DOI: 10.6001/biologija.v66i2.4256

Jana Radzijevskaja, Dovilė Tamoliūnaitė, Vytautas Sabūnas, A. Aleksandravičienė, A. Paulauskas

{"title":"Prevalence and co-infection of mosquito- and tick-borne pathogens in domestic dogs suspected for canine babesiosis in Lithuania","authors":"Jana Radzijevskaja, Dovilė Tamoliūnaitė, Vytautas Sabūnas, A. Aleksandravičienė, A. Paulauskas","doi":"10.6001/biologija.v66i2.4256","DOIUrl":"https://doi.org/10.6001/biologija.v66i2.4256","url":null,"abstract":"2 Linas Veterinary Clinic, Debreceno St. 5, Klaipėda 94175, Lithuania During the past decade, vector-borne diseases (VBDs) have been continuously spreading in Europe, including Lithuania. VBDs are caused by bacteria, parasites, or viruses transmitted by the bite of hematophagous arthropods (mainly ticks and mosquitoes). Canine vector-borne diseases (CVBDs) are a growing global threat. Since the majority of these diseases have a zoonotic potential, their management requires a multidisciplinary approach. Global warming and simplified rules for travelling with animal companions provide ideal conditions for the circulation and spreading of vector-borne pathogens in non-endemic geographical regions. Information on CVBD agents at the local and regional levels allows veterinarians to better recognize the pathogens that can affect dogs, thus facilitating diagnosis and treatment. The aim of the present study was to investigate the prevalence and co-infection of mosquitoand tick-borne pathogens in domestic dogs using molecular DNA analysis methods. Blood samples were collected from dogs presented at different veterinary clinics in six regions of Lithuania. A total of 100 blood samples from dogs suspected for canine babesiosis were screened for the presence of tick-borne pathogens Anaplasma phagocytophilum, Borrelia spp., Babesia canis and mosquito-borne pathogens Dirofilaria spp. Results of real-time PCR analysis demonstrated the presence of D. repens in 23.0%, A. phagocytophilum in 35.0%, Babesia spp. in 81.0%, and Borrelia spp. in 19.0% of examined dogs. Double, triple, or even quadruple co-infections were detected. The present study is the first investigation of multiple vector-borne pathogens in dogs from Lithuania using molecular detection methods. Our findings demonstrate a high infection rate of vector-borne pathogens in dogs and suggest that co-infections with anaplasmosis, borreliosis, babesiosis, and dirofilariosis in dogs are expected in Lithuania.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77089792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effects of UVA and its simultaneous action with blue light on the growth and phototropism of cress leaves under various gravity conditions 不同重力条件下UVA及其与蓝光同时作用对芥蓝叶片生长和向光性的影响

Biologija Pub Date : 2020-06-13 DOI: 10.6001/biologija.v66i2.4257

Regina Losinska-Sičiūnienė, R. Stanevičienė, D. Švėgždienė

{"title":"Effects of UVA and its simultaneous action with blue light on the growth and phototropism of cress leaves under various gravity conditions","authors":"Regina Losinska-Sičiūnienė, R. Stanevičienė, D. Švėgždienė","doi":"10.6001/biologija.v66i2.4257","DOIUrl":"https://doi.org/10.6001/biologija.v66i2.4257","url":null,"abstract":"Nature Research Centre, Akademijos 2, Vilnius 08412, Lithuania The gravity of the Earth (1g) and phototropic active components of the light spectrum are the stimuli regulating the directional growth of plants. In this study, the role of combined light and gravity effects on plant leaves (Lepidium sativum L.) was tested. Treatment with UVA (370 nm) or UVA combined with blue light together with the gravity in the slow clinostat (clinorotated 3 rpm), or Earth’s gravity (1g), was applied. A custom-built clinostat with LEDs allowed unidirectional illumination of cress leaves. UVA or UVA with blue light was directed laterally at cress leaves for a 3 h exposure. Responses of cotyledons and true leaves, both under 1g and clinorotation conditions, were compared. The obtained data show that UVA (370 nm) suppresses the elongation of cotyledons and true leaves under changed gravity of the Earth. Clinorotation suppresses the growth of cotyledons but not of the true leaves under UVA with blue light. Comparison of leaf phototropism induced by different illuminations under1g and changed gravity conditions revealed that phototropism was induced by clinorotation but suppressed by Earth’s gravity. Phototropic responses of true leaves under clinorotation were stronger than those of cotyledons.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74208206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure of the breeding bird community along the urban gradient in a town on the Zambezi River, north-eastern Namibia 纳米比亚东北部赞比西河上一个城镇沿城市梯度的繁殖鸟类群落结构

Biologija Pub Date : 2020-03-29 DOI: 10.6001/biologija.v66i1.4185

G. Kopij

{"title":"Structure of the breeding bird community along the urban gradient in a town on the Zambezi River, north-eastern Namibia","authors":"G. Kopij","doi":"10.6001/biologija.v66i1.4185","DOIUrl":"https://doi.org/10.6001/biologija.v66i1.4185","url":null,"abstract":"2 Department of Integrated Environmental Science, Ogongo Campus, University of Namibia, Private Bag 5520 Oshakati, Namibia A simplified mapping method was employed to quantify avian assemblages in three study plots in a modified riparian forest on the Zambezi River in the town of Katima Mulilo, NE Namibia. The plots were arranged along the urbanization gradient: plot A (34 ha; 0–1 km from centre of the town), plot B (27 ha; 1–2 km from the centre), and plot C (24 ha; 2–3 km from the centre; periphery). In total, 51 breeding bird species were recorded in all three plots. Five of them, Columba livia domestica, Passer diffusus, Pycnonotus tricolor, Streptopelia senegalensis, and Uraeginthus angolensis were classified as dominant species. The cumulative dominance was 69.9%. The overall population density of all breeding species increased with the urban gradient. In general, granivorous birds were by far the most numerous feeding guild comprising 68.7% of all breeding birds. Two other guilds, frugivorous and insectivorous birds, together comprised 28.9%. While the proportion of granivores decreased along the urban gradient from the town centre to its periphery, the proportion of insectivores increased. The proportion of birds nesting in/on buildings decreased, and the share of tree/shruband hole-nesting birds increased along the urban gradient. The diversity indices were relatively high in all plots. They decreased only slightly along the urban gradient (from the centre to the periphery). However, the Pielou’s Evenness Index was comparatively low, but on a slight increase along the urbanization gradient. The Sorensen Similarity Index was low, but the values of the three studied plots were very similar.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89446868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Extract of oats as a modulator of fatty acid composition of geese tissues in the conditions of physiological stress 生理应激条件下燕麦提取物对鹅组织脂肪酸组成的调节作用

Biologija Pub Date : 2020-03-29 DOI: 10.6001/biologija.v66i1.4188

O. Danchenko, Олена Олександрівна Данченко, Елена Александровна Данченко, Liubov Zdorovtseva, Любов Миколаївна Здоровцева, Любовь Николаевна Здоровцева, O. Vishchur, О. Koshelev, Tetiana Halko, M. Danchenko, Микола Миколайович Данченко, Николай Николаевич Данченко, Y. Nikolayeva, Daniil Maiboroda, Данііл Олександрович Майборода, Даниил Александрович Майборода

{"title":"Extract of oats as a modulator of fatty acid composition of geese tissues in the conditions of physiological stress","authors":"O. Danchenko, Олена Олександрівна Данченко, Елена Александровна Данченко, Liubov Zdorovtseva, Любов Миколаївна Здоровцева, Любовь Николаевна Здоровцева, O. Vishchur, О. Koshelev, Tetiana Halko, M. Danchenko, Микола Миколайович Данченко, Николай Николаевич Данченко, Y. Nikolayeva, Daniil Maiboroda, Данііл Олександрович Майборода, Даниил Александрович Майборода","doi":"10.6001/biologija.v66i1.4188","DOIUrl":"https://doi.org/10.6001/biologija.v66i1.4188","url":null,"abstract":"3 Institute of Animal Biology of NAAS, 38 V. Stusa St., Lviv 79034, Ukraine Feeding natural antioxidant supplements to animals and birds has multiple advantages over traditional synthetic vitamins of the antioxidant group. This study investigated the effect of the Avena satíva extract on the antioxidant status and fatty acid composition of liver tissues, the brain, and skeletal muscles of geese, live weight dynamics, and pterylographic parameters during physiological stress of formation of contour and juvenile feathers. It is confirmed that adding oat extract to the geese diet during physiological stress increases tissue antioxidant activity. It was established that during the formation of contour feathers (day 28), the voltage of the antioxidant system is significantly weakened by the action of the extract due to selective inhibition of the synthesis of unsaturated fatty acids, in particular oleic. The synthesis of palmitic and stearic acids is activated. The oat extract caused the most remarkable changes in liver tissues. The subsequent period of formation of juvenile feathers (49 days) is characterized by equalization of the composition of fatty acids in the control and experimental groups. At the end of the experiment, the mass of the geese of the experimental group increased by 17.9% and their pterylographic parameters improved.","PeriodicalId":8858,"journal":{"name":"Biologija","volume":"20 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89061167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Diagnostics based on the juxta morphology reveals a new species of Tischeria Zeller (Lepidoptera, Tischeriidae) from the Caucasus 基于近群形态学的诊断揭示了高加索地区一新种(鳞翅目，鳞翅目蝇科)

Biologija Pub Date : 2020-03-29 DOI: 10.6001/biologija.v66i1.4186

Adam Klasiński, J. R. Stonis, A. Remeikis

引用次数: 3