Biotechnology and Health Sciences最新文献_第10页

The Assessment of Dentists’ Knowledge Regarding Indications of Cone Beam Computed Tomography in Qazvin, Iran 伊朗Qazvin牙医对锥形束计算机断层扫描适应症的知识评估

Biotechnology and Health Sciences Pub Date : 2015-02-21 DOI: 10.17795/BHS-25815

M. Tofangchiha, F. Arianfar, M. Bakhshi, M. Khorasani

{"title":"The Assessment of Dentists’ Knowledge Regarding Indications of Cone Beam Computed Tomography in Qazvin, Iran","authors":"M. Tofangchiha, F. Arianfar, M. Bakhshi, M. Khorasani","doi":"10.17795/BHS-25815","DOIUrl":"https://doi.org/10.17795/BHS-25815","url":null,"abstract":"Background: Cone beam computed tomography (CT) has recently become effective for oral and maxillofacial imaging. \u0000Objectives: The aim of this study was to assess the knowledge of dentists regarding cone beam computed tomography. \u0000Materials and Methods: In this descriptive cross sectional study, a questionnaire regarding cone-beam computed tomography (CBCT) was distributed amongst 100 dentists (general and specialist) in Qazvin, Iran. Their level of knowledge was compared in each section on the basis of age, gender, years of employment and last educational status and analyzed by the SPSS software and Mann-Whitney test. \u0000Results: Data analysis showed that 4% of dentists had very low, 16% had low, 50% had medium, 19% had good and 11% had in very good level of knowledge. The average of dentists’ knowledge was 57 ± 18. According to the statistical results, there was a significant difference between level of knowledge and age, years of employment and educational degree (P 0.05). \u0000Conclusions: Overall, dentists had an average level of knowledge for CBCT. It is recommended for qualification programs to be held for dentists to strengthen their awareness toward cone beam computed tomography. \u0000Keywords:Knowledge; Dentist; Cone - Beam Computed Tomography","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89925960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Frequency of Chlamydia trachomatis in Endocervical Samples of Women Referred to a Gynecology Hospital in Qazvin, Iran 伊朗加兹温一家妇科医院妇女宫颈内样本沙眼衣原体的频率

Biotechnology and Health Sciences Pub Date : 2015-02-21 DOI: 10.17795/BHS-27009

M. Aslanimehr, M. S. Ghazvini, Saman Saadat, A. Barikani, T. Farivar

引用次数: 2

The Effects of Mothers' Self-Efficacy on Children's Physical Activity 母亲自我效能感对儿童体育活动的影响

Biotechnology and Health Sciences Pub Date : 2015-01-31 DOI: 10.17795/BHS-25731

K. Hosseinzadeh, S. Niknami, A. Hidarnia

引用次数: 12

Expression of Cucumber Mosaic Virus Coat Protein and Its Assembly into Virus Like Particles 黄瓜花叶病毒外壳蛋白的表达及其组装成病毒样颗粒

Biotechnology and Health Sciences Pub Date : 2014-11-30 DOI: 10.17795/BHS-24729

A. Rostami, N. S. Bashir, Parviz Pirniakan, N. Masoudi

引用次数: 7

Evaluation of Antioxidant, Antibacterial, and Antifungal Properties of Satureja hortensis Essential Oil 荆芥精油抗氧化、抗菌和抗真菌性能的评价

Biotechnology and Health Sciences Pub Date : 2014-11-30 DOI: 10.17795/BHS-24733

S. Valizadeh, Tayebeh Fakheri, R. Mahmoudi, F. Katiraee, P. Gajarbeygi

{"title":"Evaluation of Antioxidant, Antibacterial, and Antifungal Properties of Satureja hortensis Essential Oil","authors":"S. Valizadeh, Tayebeh Fakheri, R. Mahmoudi, F. Katiraee, P. Gajarbeygi","doi":"10.17795/BHS-24733","DOIUrl":"https://doi.org/10.17795/BHS-24733","url":null,"abstract":"Background: Satureja is a genus belonging to the aromatic plants of Lamiaceae family. The genus Satureja L. (Lamiaceae) comprises more than 30 species of aromatic herbs and shrubs, widely distributed over the Mediterranean region. The genus is represented by 14 species in Iran of which, eight are endemic. Many species of the genus Satureja are reported to have aromatic and medicinal properties. The leaves, flowers, and stems of this plant are used as herbal tea and in treatment of various ailments in traditional medicine. Objectives: The aim of this study was to investigate antimicrobial and antioxidant activity of essential oil (EO) of Satureja hortensis (Lamiaceae) that grows in Sabalan Mountain (Ardebil province, Iran). Materials and Methods: This EO was tested in vitro against two bacterial species by disk and agar well diffusion methods and against four bacterial species and three Candida strains by broth microdilution method. Total phenol, flavonoid, and free radical scavenging activity of EO were evaluated. Results: Total phenolic content was determined by using Folin-Ciocalteu reagent (32.65 mg/g), which was presented as gallic acid equivalent in 1 g of sample. IC50 of EO and ascorbic acid in DPPH method were respectively 277.9 and 19.34 µg. Minimum inhibitory concentration of the EO of S. hortensis against Salmonella typhimurium, Listeria monocytogenes, Escherichia coli, and Bacillus cereus was respectively 2.5%, 2.5%, 5%, and 2.5%. The inhibition zone of EO in disk and agar well diffusion method showed that inhibitory zone on B. cereus was higher than that on S. typhimurium in both methods and B. cereus was more sensitive to EO. MIC to minimal fungicidal concentration (MFC) ratio of S. hortensis EO against Candida albicans, Candida parapsilosis, and Candida krusei in broth microdilution method were respectively 0.048%:0.048%, 0.024%:0.024%, and 0.012%:0.012%. Conclusions: The data of the study clearly indicated that the EO of S. hortensis has a strong antioxidant, antibacterial, and antifungal activity.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85442885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Epidemiological Study of Non-Melanoma Skin Cancers Qazvin Province, Iran 伊朗Qazvin省非黑色素瘤皮肤癌流行病学研究

Biotechnology and Health Sciences Pub Date : 2014-11-30 DOI: 10.17795/BHS-25362

Akram Beheshtiroy, F. Hajmanoochehri

{"title":"Epidemiological Study of Non-Melanoma Skin Cancers Qazvin Province, Iran","authors":"Akram Beheshtiroy, F. Hajmanoochehri","doi":"10.17795/BHS-25362","DOIUrl":"https://doi.org/10.17795/BHS-25362","url":null,"abstract":"and statistical tests including t-test and chi-square tests. Results: Of the 484 patients, 294 (60.7%) were male and 190 (39.3%) were female. Most cases of carcinoma were found in basal cells (77.7%) and the rest of squamous cells. The most common sites of involvement were the head and scalp. The most frequent predisposing factors were working in the open air (70.7%), history of previous radiation for treatment of tenia capitis (26.1%), and chronic skin diseases such as burn eschar, chronic lesion, fistula and actinic keratosis (35.7%). The most prevalent Fitzpatrick skin phenotypes were II or III (75.4%). There were significant correlations between the incidence of NMSC and hookah smoking and oral contraceptive pill (OCP) consumption. Conclusions: Skin phenotypes II and III were the most common types found in the present study and this could be due to the higher frequency of these phenotypes among the study population. The lower incidence of NMSC in areas other than the neck and scalp could be associated with the importance of covering style used by the population under study. Known risk factors for the incidence of NMSC were also observed in the present study. A history of radiotherapy for treatment of tenia capitis was observed in a considerable percentage of patients, which could lead to the incidence of cancer several decades later. Further studies are needed to determine the role of hookah smoking and the use of OCP in the occurrence of NMSC.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81686598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Role of Calcium in Calprotectin Dimerization as a Cancer Biomarker 钙在钙保护蛋白二聚化中作为癌症生物标志物的作用

Biotechnology and Health Sciences Pub Date : 2014-11-28 DOI: 10.17795/BHS-26379

F. N. Nikoo, K. G. Chegini, R. N. Pour, N. Gheibi

{"title":"The Role of Calcium in Calprotectin Dimerization as a Cancer Biomarker","authors":"F. N. Nikoo, K. G. Chegini, R. N. Pour, N. Gheibi","doi":"10.17795/BHS-26379","DOIUrl":"https://doi.org/10.17795/BHS-26379","url":null,"abstract":"Background: S100A8 and S100A9 as two subunits of heterodimeric calprotectin are identified mainly in leukocytes and are involved in inflammatory processes and several cancerous pathogens. This study was performed in order to evaluate the interaction of recombinant calprotectin subunits and to estimate calprotectin’s tertiary and secondary structures. Objectives: The aim of this study was to investigate the effects of calcium in calprotectin dimerization as a cancer biomarker. Materials and Methods: Heterodimeric calprotectin was formed with incubation of recombinant S100A8 and S100A9 subunits in the presence of Ca (1 mM), at 25 C for 15 minutes. Tertiary and secondary structures of S100A8, S100A9 and their complex were investigated, using fluorescence and circular dichroism (CD) spectroscopy, respectively. Results: Interaction of S100A8 and S100A9 in the presence of Ca2+ were revealed by decreasing the emission intensity of intrinsic fluorescence and increasing of the external fluorescence and also changes in the CD spectra of subunits after Ca2+ interactions. Conclusions: The expression of recombinant calprotectin, as an effective protein, can help in diagnosis or treatment of inflammatory and cancer processes in the future. Furthermore, Ca2+ induced a partial change in secondary and tertiary structure of calprotectin subunits and this change is probably necessary for protein dimerization.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82647252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prevalence of Helicobacter pylori in Formaldehyde Fixed Paraffin Embedded Gastric Tissues of Gastric Cancer Patients by Scorpion Real-Time PCR Assay Real-Time PCR法检测胃癌患者胃组织中幽门螺杆菌的含量

Biotechnology and Health Sciences Pub Date : 2014-11-28 DOI: 10.17795/BHS-25836

A. Farzam, R. Najafipour, P. Johari, T. Farivar

{"title":"Prevalence of Helicobacter pylori in Formaldehyde Fixed Paraffin Embedded Gastric Tissues of Gastric Cancer Patients by Scorpion Real-Time PCR Assay","authors":"A. Farzam, R. Najafipour, P. Johari, T. Farivar","doi":"10.17795/BHS-25836","DOIUrl":"https://doi.org/10.17795/BHS-25836","url":null,"abstract":"Background: Gastric cancer is the second leading cause of cancer-related deaths worldwide and it seems that environmental and lifestyle factors and infection with Helicobacter pylori (H. pylori) have a major role in the etiology of gastric cancer. Objectives: The aim of this study was to investigate the presence of H. pylori DNA in archival gastric tissues of patients with gastric cancer disease by rapid, sensitive and specific technique of Scorpion real-time PCR. Patients and Methods: This retrospective cross-sectional study was performed during the year 2009, on 285 paraffin embedded gastric specimens of patients, who were pathologically proved to have gastric cancer and were admitted to Bou-Ali, Shahid Rajaie and Dehkhoda Hospitals and Bahar and Farzam Private Laboratory of Qazvin city, Iran. Results: The results of the Scorpion real-time PCR showed that H. pylori DNA was present in 8.42% of the total specimens. Modified McMullen’s staining of paraffin embedded sections were positive in ten patients. There was no significant relationship between the presence of H. pylori, sex, age and place of residence. Conclusions: Although the existence of H. pylori in gastric tissue samples of patients with gastric cancer is controversial however, our results showed that in our studied specimens a significant number of patients with gastric cancer had H. pylori colonization.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77376525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibitory Effects of a Palladium Complex on the Activity, Stability, and Structure of Tyrosinase Enzyme 钯配合物对酪氨酸酶活性、稳定性和结构的抑制作用

Biotechnology and Health Sciences Pub Date : 2014-11-28 DOI: 10.17795/BHS-26373

N. Gheibi, Nasibe Yaghouby Nejad, M. Sahmani

引用次数: 0

Novel Real Time Polymerase Chain Reaction Approach for Rapid Detection of the Residual Escherichia coli Genomic DNA in Biopharmaceutical Products Establishment of Real Time Polymerase Chain Reaction to Detect Residual gDNA 新型实时聚合酶链式反应快速检测生物制药产品中残留大肠杆菌基因组DNA的方法

Biotechnology and Health Sciences Pub Date : 2014-11-28 DOI: 10.17795/BHS-26375

T. Farivar, Babak Mamnoon, M. K. Arzenani, D. Ilghari

{"title":"Novel Real Time Polymerase Chain Reaction Approach for Rapid Detection of the Residual Escherichia coli Genomic DNA in Biopharmaceutical Products Establishment of Real Time Polymerase Chain Reaction to Detect Residual gDNA","authors":"T. Farivar, Babak Mamnoon, M. K. Arzenani, D. Ilghari","doi":"10.17795/BHS-26375","DOIUrl":"https://doi.org/10.17795/BHS-26375","url":null,"abstract":"Background: Contamination of therapeutic recombinant proteins with residual host cell DNA must be controlled under the regulatory standards. Objectives: The current study established a new rapid, sensitive real time polymerase chain reaction (PCR) approach to measure the reliably of the residual Escherichia coli (E. coli) host cell genomic DNA in the recombinant streptokinase and alfa interferon preparations. Materials and Methods: In this assay, a specific primer pair was utilized to amplify a 115 base pair sequence inside the E. coli 16S rRNA using SYBR Green Chemistry. This method enabled the authors to detect a very small quantity of the residual genomic DNA, as low as 0.8 pg, in the protein-based drugs. This method can, therefore, offer a dependable way to quantitatively analyze the major contaminant of biopharmaceutical products, the host cell DNA, during the manufacturing process. Results: SYBR Green PCR master mix may contain a source of DNA contamination during its manufacturing process. Conclusions: The current study data showed that E. coli host cell DNA contamination in streptokinase and alfa interferon manufactured in the Pasteur institute of Iran is much lower than the safety limits suggested by the FDA.","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80263557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1