American journal of immunology最新文献_第8页

Immunodiagnosis in Jembrana Disease: A Review 詹布拉纳病的免疫诊断研究进展

American journal of immunology Pub Date : 2015-10-21 DOI: 10.3844/AJISP.2015.102.107

A. Kusumawati, Tenri Ashari Wanahari, W. Asmara, S. A. Prihatno, Basofi Ashari Mappakaya, B. Hariono

引用次数: 0

Factors Affecting the Response to Interferon Alpha Therapy in Egyptian HCV Patients 影响埃及HCV患者对干扰素治疗反应的因素

American journal of immunology Pub Date : 2015-09-30 DOI: 10.3844/AJISP.2015.92.101

N. Alhusseini, F. Serry, A. A. Kadry, H. Khairy

{"title":"Factors Affecting the Response to Interferon Alpha Therapy in Egyptian HCV Patients","authors":"N. Alhusseini, F. Serry, A. A. Kadry, H. Khairy","doi":"10.3844/AJISP.2015.92.101","DOIUrl":"https://doi.org/10.3844/AJISP.2015.92.101","url":null,"abstract":"Hepatitis C Virus (HCV) infection is one of the main causes of chronic liver disease worldwide. Egypt has the highest prevalence of Hepatitis C Virus (HCV) in the world, estimated nationally at 14.7%. knowledge of the predictors of Sustained Viral Response (SVR) to Pegylated Interferon (PEG-INF) and Ribavirin (RBV) therapy in patients with chronic hepatitis C is crucial for selecting patients who would benefit most from therapy especially in developing country as Egypt where the highest percentage of treatment cost funded by government. This study was performed on Egyptian patients with chronic active hepatitis C who were prepared for receiving a course of treatment with: Interferon (PEG IFN) and ribavirin for 48 weeks. We aimed to find more predictive markers those can help clinicians to choose the most effective treatment program for each patient. Assessment of HLA-DRB1, HLA-DQB1 genes by Sequence Specific Primer (SSP) PCR, interleukin-10 (IL-10) by ELISA technique and Serum HCV RNA load by TaqMan Real Time RT-PCR technology were done. We found that sustained responders were significantly among DRB1*13 allele and DQB1*02 typing (p<0.001). DRB1*07 allele only appeared with responded patients. Relations were found between response to treatment and lower IL-10 level and lower Body Mass Index (BMI) but not sufficient to reach to significant value. In conclusion, we emphasize the importance of the use of pharmacogenomic data in the treatment of patients. HLA-DRB1*07, HLA-DRB1*13 and HLA-DQ02 alleles can predict the response to HCV combined therapy. The data generated from our study may be helpful in future for clinicians to predict the treatment outcome for HCV-seropositive individuals in Egypt.","PeriodicalId":88361,"journal":{"name":"American journal of immunology","volume":"11 1","pages":"92-101"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AJISP.2015.92.101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70190360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation the Synergistic Effect of High Dose Radiation of Radioiodine on the Immune System Suppressed By Cyclosporine 高剂量放射性碘对环孢素抑制免疫系统协同作用的评价

American journal of immunology Pub Date : 2015-09-28 DOI: 10.3844/AJISP.2015.85.91

M. Alavi, Mohammad Ali Okhovat, Kourosh Bamdad, M. Motazedian, F. Ebadi, A. Movahedpour, Faezeh Hekmatara, M. Atefi

{"title":"Evaluation the Synergistic Effect of High Dose Radiation of Radioiodine on the Immune System Suppressed By Cyclosporine","authors":"M. Alavi, Mohammad Ali Okhovat, Kourosh Bamdad, M. Motazedian, F. Ebadi, A. Movahedpour, Faezeh Hekmatara, M. Atefi","doi":"10.3844/AJISP.2015.85.91","DOIUrl":"https://doi.org/10.3844/AJISP.2015.85.91","url":null,"abstract":"Cyclosporine is an immunosuppressant drug and iodine-131 is the diagnostic and therapeutic radioisotope in nuclear medicine. In this study, we aimed to evaluate the synergistic effect of high-dose radiation of radioiodine on the suppression of the immune system of BALB/cmice after receiving cyclosporine. A total of 50 mice BALB/c were randomly divided into two groups (control and test). Then, 50 mg kg-1 body weight cyclosporine was given to both groups. After 24 h, 0.5 mL iodine-131 was given to the test group and in the control group the same volume of normal saline was given. Data analysis was done using SPSS V.20 and p value <0.05 was considered significant. After 30 days of monitoring, only 2 deaths (8%) occurred in the control group, however 16 deaths (64%) were observed in the test group. Statistical analysis of the data indicated that there is a significant relationship (p value <0.0005) between injecting radioiodine and the mortality rate in mice receiving cyclosporine and proves the synergistic effect of these two factors. Based on the results, iodine-131 can be introduced as a stimulating and synergistic factor on the suppression of the immune system of BALB/cmice and subsequent death.","PeriodicalId":88361,"journal":{"name":"American journal of immunology","volume":"11 1","pages":"85-91"},"PeriodicalIF":0.0,"publicationDate":"2015-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AJISP.2015.85.91","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70190349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Genomic Signature of Respiratory Microbes on Human Scalp Epithelia 人头皮上皮呼吸微生物的基因组特征

American journal of immunology Pub Date : 2015-09-11 DOI: 10.3844/AJISP.2015.74.84

B. Paul, Vani Mishra, J. Chhabra, Divyani Paul

{"title":"Genomic Signature of Respiratory Microbes on Human Scalp Epithelia","authors":"B. Paul, Vani Mishra, J. Chhabra, Divyani Paul","doi":"10.3844/AJISP.2015.74.84","DOIUrl":"https://doi.org/10.3844/AJISP.2015.74.84","url":null,"abstract":"Despite overlap between habitats occupied by diverse Airborne Respiratory Microbes (ARM), the airway epithelium is known for respiratory microbe infections, human scalp epithelium is undocumented. Analogous to airways epithelium, the scalp epithelium is vulnerable to infection by dormant variants of sensitive microbes that underlie many chronic and relapsing diseases, difficult to eradicate by conventional antibiotics and challenged our ability to treat chronic infection. Hence comprehensive understanding of scalp epithelium ARM load, its disposal and impact on the host, as well as the environment, remains to be defined; we need to know the portals of entry and the virulence potential of ARM in determining the impact on human health and well beings. Here we investigate the genomic signature of diverse ARM in inflammatory scalp programmed for desquamation. Spatial analysis of Mycobacterium tuberculosis (Mtb), Chlamydophila pnumoniae (Cp), Escherichia coli (E. coli), Respiratory Syncytial virus strain A/B (RSVA/RSVB) and Influenza virus (H1N1) in the Flakes of White Scales (FWS) from the hair bearing areas of the scalp by quantitative (q) PCR reveals flux of microbes ranging from ~106 to 108 copies/ng FWS-DNA. Mtb and 16SrRNA identity reaffirmed by amplicon sequencing. Absence of microbe gene expression signatures in FWS negates the presence of viable ARM, absence of E. coli adhesin mRNA indicates habitat rejection in coculture of epithelial cells and E. coli JM107 and absence of H1N1 hemaglutinin1 (H1) mRNA in FWS rules out canonical binding and downstream infection of neighboring cell by H1N1. We conclude that desquamated epithelium, in addition to Malassezia sp, bear diverse genomic signatures of non-virulent respiratory microbes, suggest an undefined portal of entry, helps in clearance of microbes and possess minimum risk of re-infection to neighboring cells and subjects.","PeriodicalId":88361,"journal":{"name":"American journal of immunology","volume":"11 1","pages":"74-84"},"PeriodicalIF":0.0,"publicationDate":"2015-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AJISP.2015.74.84","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70190307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccine against Jembrana Disease Virus Infection: A Summary Findings 詹布拉那病病毒感染疫苗研究综述

American journal of immunology Pub Date : 2015-09-02 DOI: 10.3844/AJISP.2015.68.73

A. Kusumawati, Tenri Ashari Wanahari, P. Astuti, Kurniasih, Basofi Ashari Mappakaya, H. Wuryastuty

引用次数: 1

The Potential of VipAlbuminÂ® to Chronic Inflammation in Type 2 Diabetes Mellitus Balb/C Mice Model VipAlbuminÂ®对2型糖尿病Balb/C小鼠慢性炎症的影响

American journal of immunology Pub Date : 2015-07-13 DOI: 10.3844/AJISP.2015.56.67

D. Dwijayanti, M. S. Djati, Mansur Ibrahim, Muhaimin Rifai

{"title":"The Potential of VipAlbuminÂ® to Chronic Inflammation in Type 2 Diabetes Mellitus Balb/C Mice Model","authors":"D. Dwijayanti, M. S. Djati, Mansur Ibrahim, Muhaimin Rifai","doi":"10.3844/AJISP.2015.56.67","DOIUrl":"https://doi.org/10.3844/AJISP.2015.56.67","url":null,"abstract":"Diabetes Mellitus (DM) is one of diseases which have increasing number of sufferers every year. Almost all DM patients are type 2 DM. One of the causes of type 2 DM is a chronic inflammation due to an increase of circulating proinflammatory cytokines such as TNF-I±, IFN-I³ and IL-6. VipAlbuminÂ® from snakehead fish extract is expected to be useful as an alternative treatment of type 2 DM because of its proinflammatory activity. The aim of this study was to determine the effect of VipAlbuminÂ® on regulatory T cell activation, macrophage cells, proinflammatory cytokines and NF-IoB. The experiments were done by inducing mice model of type 2 DM with STZ 100 mg/kg BW and then gave them oral administration of VipAlbuminÂ® 0 mg/g BW, 0.01664 mg/g BW, 0.0416 mg/g BW and 10.4 mg/g BW for 14 days. The data were statistically analyzed with one way ANOVA with significance value 0.05% and Tukey test using SPSS version 16 for Windows. The results showed the decreasing number of regulatory T cells in type 2 DM mice, increasing number of macrophage cells and proinflammatory cytokines TNF-I±, IFN-I³ and IL-6 as well as the increasing number of NF-IoB as a transcription factor of inflammatory mediators in CD4+ T cells, CD8+ T cells and macrophages (CD68+) compared to healthy mice (pâ¤0.05). Oral administration of VipAlbuminÂ®for 14 days was proven to cure type 2 DM mice (K+) by increasing the number of regulatory T cells, decreasing the number of macrophage cells and proinflammatory cytokines TNF-I±, IFN-I³ and IL-6 and inhibiting NF-IoBin T lymphocytes CD4+, CD8+ and macrophages at the level equivalent to healthy mice and significantly different (p","PeriodicalId":88361,"journal":{"name":"American journal of immunology","volume":"11 1","pages":"56-67"},"PeriodicalIF":0.0,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AJISP.2015.56.67","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70190258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of Unilateral Cervical Vagotomy on Murine Dendritic Cells 单侧颈部迷走神经切断术对小鼠树突状细胞的影响

American journal of immunology Pub Date : 2015-07-12 DOI: 10.3844/AJISP.2015.48.55

D. Cruz, A. P. Lima, P. B. G. Silva, J. Palermo-neto, C. Massoco

{"title":"Effects of Unilateral Cervical Vagotomy on Murine Dendritic Cells","authors":"D. Cruz, A. P. Lima, P. B. G. Silva, J. Palermo-neto, C. Massoco","doi":"10.3844/AJISP.2015.48.55","DOIUrl":"https://doi.org/10.3844/AJISP.2015.48.55","url":null,"abstract":"The cholinergic anti-inflammatory pathway has been demonstrated to be an important mechanism that modulates several inflammatory diseases. This modulation occurs upon activation of the vagus nerve, which modulates splenic macrophages and lymphocytes through the predominant neurotransmitter, Acetylcholine (ACh). We hypothesized that this modulation could also take place in dendritic cells, as these cells represent a link between the innate and adaptive immune responses. Bone Marrow-Derived Dendritic Cells (BMDCs) were generated from mice subjected to a unilateral cervical vagotomy and the phenotypes and functions of the BMDCs were evaluated. No significant effects were found in BMDCs from vagotomized mice compared to cells from the control group. The specific immune response was evaluated with the Delayed-Type Hypersensitivity (DTH) skin test using Keyhole Limpet Hemocyanin (KLH) as an antigen and the peak of the response in the vagotomized animals was delayed compared to the control group. It is possible that the Dendritic Cells (DCs) can be modulated by the vagus nerve in other specific sites or under challenging conditions, but our work suggests that immune cells could be modulated by the components of another cholinergic anti-inflammatory pathway or by another neuro-immune pathway interaction.","PeriodicalId":88361,"journal":{"name":"American journal of immunology","volume":"11 1","pages":"48-55"},"PeriodicalIF":0.0,"publicationDate":"2015-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AJISP.2015.48.55","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70190247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Host Receptor Immunomodulation in Response to Shigella Surface Antigens: An Insight for Vaccine Development 宿主受体对志贺氏菌表面抗原的免疫调节:对疫苗开发的一种见解

American journal of immunology Pub Date : 2015-06-21 DOI: 10.3844/AJISP.2015.33.47

A. Bagchi, R. Bagchi, D. Hens, F. Jahan, Pragna Parikh, D. Saha

{"title":"Host Receptor Immunomodulation in Response to Shigella Surface Antigens: An Insight for Vaccine Development","authors":"A. Bagchi, R. Bagchi, D. Hens, F. Jahan, Pragna Parikh, D. Saha","doi":"10.3844/AJISP.2015.33.47","DOIUrl":"https://doi.org/10.3844/AJISP.2015.33.47","url":null,"abstract":"Shigellosis, caused by Shigella is the most common cause of bacillary dysentery. The disease is associated with high morbidity and mortality rate owing to its multiple drug resistance. Hence recovery from the disease would primarily depend on the development of an effective immune-modulator for strong mucosal immune response. The role of cellular immunity may be a critical factor in protection against shigellosis as Shigella remains an intracellular pathogen during most of its life-cycle. Development of a potent immunomodulator may provide strong and long-lasting immunity to shigellosis. In this review, we have attempted to highlight the disease dimension and its deviation due to the effect of various Shigella surface antigens that would help in the development of an effective immune response. Cellular innate immune modulation will be a new generation target for the development of mucosal candidate vaccines where proper receptor activation such as Toll-Like Receptors (TLRs), Cytokine Receptors (CyRs) and/or T-Cell Receptors (TCRs) on the host cell could be aimed at producing mucosal immunity. An effort has been made to better understand the effect of these immunomodulators against shigellosis by way of modulating the host immune mechanism to Shigella outer membrane component.","PeriodicalId":88361,"journal":{"name":"American journal of immunology","volume":"11 1","pages":"33-47"},"PeriodicalIF":0.0,"publicationDate":"2015-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AJISP.2015.33.47","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70190213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Dietary Nucleotides on Immune Mechanisms and Physical State in Children with Chronic Respiratory Problems 膳食核苷酸对慢性呼吸系统疾病儿童免疫机制和身体状态的影响

American journal of immunology Pub Date : 2015-06-12 DOI: 10.3844/AJISP.2015.26.32

R. Josef, Svozil Vladimir, Král Vlastimil, Rajnohova Dobiasova Lucie, S. Ivana, Pohořská Jitka, V. Vaclav

引用次数: 5

Polyherbal EMSA ERITIN Blocks Nuclear Factor-Kappa B (NF-ÎºB) and Proinflammatory Cytokines in Irradiated Mice EMSA erittin阻断辐照小鼠核因子κ B (NF-ÎºB)和促炎细胞因子

American journal of immunology Pub Date : 2015-05-29 DOI: 10.3844/AJISP.2015.17.25

Y. Christina, Mansur Ibrahim, M. Rifa’i

引用次数: 4